請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41144完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 杜宜殷 | |
| dc.contributor.author | Ei-Long Lee | en |
| dc.contributor.author | 李宜龍 | zh_TW |
| dc.date.accessioned | 2021-06-14T17:19:49Z | - |
| dc.date.available | 2011-01-01 | |
| dc.date.copyright | 2008-08-04 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-24 | |
| dc.identifier.citation | 柒、參考文獻
郭純德. 1987. 苦瓜果實採收成熟與採後生理之研究. 台灣大學園藝系碩士論文. 張綺恂. 2002. 乙烯、黑暗貯運及 1-MCP 對蝴蝶蘭盆花品種產後品質開花之影 響. 台灣大學園藝系碩士論文. 蔡幸君. 2004. 四種園藝作物EIN3同源cDNA之選殖與分析. 台灣大學園藝系 碩士論文. Adie, B., J. M. Chico, I. Rubio-Somoza, and R. Solano. 2007. Modulation of plant defenses by ethylene. J Plant Growth Regul. 26:160-177 Alonso, J. M., T. Hirayama, G. Roman, S. N ourizadeh, and J. R. Ecker. 1999. EIN2, a bifunctional transducer of ethylene and stress response in Arabidopsis. Science 284:2148-2151. Bleecker, A. B., M. A. Estelle, C. Somerville, and H. Kende. 1998. Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 241:1086-1089. Binder, B. M., J. M. Walker, J. M. Gagne, T. J. Emborg, G. Hemmann, A. B. Bleecker, and R. D. Vierstrab. 2007. The Arabidopsis EIN3 Binding F-Box Proteins EBF1 and EBF2 have distinct but overlapping roles in ethylene signaling. Plant Cell 19:509-523. Chan, H. T. 1986. Heat inactivation of the ethylene-forming enzyme system in cucum- ber. J. Food Sci. 51:1491-1493. Chang, C. and R. Standler. 2001. Ethylene hormone receptor action in Arabidopsis. Bioessays 23:619-627. Chao , Q., M. Rothenberg, R. Solamo, G. Roman, W. Terzaghi, and J. R. Ecker. 1997. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell 89:1133-1144. Chen , Y. F., N. Etheridge, and G. E. Schaller. 2005. Ethylene signal transduction. Ann. Bot. 95: 901-915. Chen, Y. F., M. D. Randlett, J. L. Findell, and G. E. Schaller. 2002. Localization of the ethylene receptor ETR1 to the endoplasmic reticulum of Arabidopsis. J. Biol. Chem. 277: 19861-19866. Diaz-De-Leon, F., K. L. Klotz, and M. Lagrimini. 1993. Nucleotide sequence of the tobacco (Nicotiana tabacum) anionic peroxidase gene. Plant Physiol. 101:1117 -1118. Du, J., X. Wang, M. Zhang, D. Tian, and Y. H. Yang. 2007. Unique nucleotide poly- morphism of ankyrin gene cluster in Arabidopsis. J. Genetics. 86:27-35. Ecker, J. R. 1995. The ethylene siganal transduction pathway in plants. Science 268: 667-675. Gao, Z., Y. F. Chen, M.D. Randlett, X. C. Zhao, J. L. Findell, J. J. Kieber , and G. E. Schaller. 2003. Localization of the Raf-like kinase CTR1 to the endoplasmic reticulum of Arabidopsis through participation in ethylene receptor signalling complexes. J. Biol. Chem. 278: 34725–34732. Grec, S., D. Vanham, J. C. de Ribaucourt, B. Purnelle, and M. Boutry. 2003. Identi- fication of regulatory sequence elements within the transcription promoter region of NpABC1, a gene encoding a plant ABC transporter induced by diterpenes. Plant J. 35:237-250 Gou, H. and J. R. Ecker. 2003. Plant responses to ethylene gas are mediated by SCF EBF1/EBF2-dependent proteolysis of EIN3 transcription factor. Cell 115:667-677. Guiltinan, M. J., W. R. Marcotte Jr., and R. S. Quatrano. 1998. A plant leucine zipper protein that recognizes an abscisic acid response element. Plant Mol. Biol. 37: 425-435. Hattori, T., T. Terada, and S. Hamasuna. 1995. Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1: analysis of cis-acting pro- moter elements required for regulation by abscisic acid and VP1. Plant J. 7: 913-925. Huang, Y., H. Li, C. E. Hutchison, J. Laskey, and J. J. Kieber. 2003. Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signalling in Arabidopsis. Plant J. 33:221-233. Hue, J., H. Sakai, S. Nourizadeh, Q. G. Chen, A. A. Bleecker, J. R. Ecker, and E. M. Meyerowitz. 1998. EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis. Plant Cell 10: 1321-1332. Hyodo, H., and R. Fukasawa.1985. Ethylene production in kiwi fruit. J. Japan Soc. Hort. Sci. 54:209-215. Kieber, J. J., M. Rothenberg, G. Roman, K. A. Feldmann, and J. R. Ecker. 1993. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encode member of the raf family of protein kinase. Cell 72:427-441. Korfhage, U., G. F. Trezzini, I. Meier, K. Hahlbrock, and I. E. Somssich. 1994. Plant homeodomain protein involved in transcriptional regulation of a pathogen defense-related gene. Plant cell. 6:695-708. Kosugi, S. and Y. Ohashi. 2000. Cloning and DNA-binding properties of a tobacco ethylene-insensitive 3 (EIN3) homolog. Nucleic Acid Res. 28:960-967. Lee, J. H., and W. T. Kim. 2003. Molecular and biochemical characterization of VR-EILs encoding mung bean ETHYLENE INSENSITIVE3-LIKE proteins. Plant Physiol. 132:1475-1488. Lois, R., A. Dietrich, K. Hahlbrock, and W. Schulz. 1989. A phenylalanine ammonia- lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J. 8:1641-1648. Müller, R., C. A. Owen, Z. T. Xue, M. Welander, and B. Stummann. 2003. The transcription factor EIN3 is constitutively expresses in miniature roses with difference in postharvest life. J. Hort. Sci. Biotech. 78:10-14. Nagao, R. T., V. H. Goekjian, J. C. Hong, and J. L. Key. 1993. Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean. Plant Mol. Biol. 21:1147-1162. Nakasawa, M., N. Yabe, T. Ishikawa, Y. Y. Yamamoto, T. Yoshizumi, K. Hasunuma, and M. Matsui. 2001. DFL1, an auxin-responsive GH3 gene homologue, negatively regulates shoot cell elongation and lateral root formation, and positively regulates the light response of hypocotyls length. Plant J. 25:213- 221. Olmedo, G., H. Guo, B. D. Gregory, S. D. Nourizadeh, L. Aguilar-Henonin, H. Li, F. An, P. Guzman, and J. R. Ecker. 2006. ETHYLENE-INSENSITIVE5 encodes a 5’→ 3’exoribonuclease required for regulation of the EIN3-targeting F-box proteins EBF1/2. Proc. Natl. Acad. Sci. USA 103:13286-13293. Ouaked, F., W. Rozhon, D. Lecourieux, and H. Hirt. 2003. A MAPK pathway mediates ethylene siganaling in plant. EMBO J. 22:1282-1288. Pastuglia, M., D. Roby, C. Dumas, and J. M. Cock. 1997. Rapid induction by wounding and bacterial infection of an S gene family receptor-like kinase in Brassica olerace. Plant Cell 9:1-13. Potuschak, T., A. Vansiri, B. M. Binder, E. Lechner, R. D. Vierstra, and P. Genschik. 2006. The exoribonuclease XRN4 is a component of the ethylene response pathway in Arabidopsis. Plant Cell 18:3047–3057. Potuschak, T., E. Lechner, Y. Parmentier, S. Yanagisawa, S. Grava, C. Koncz, and P. Genschik. 2003. EIN3-dependent regulation of plant ethylene hormone signal- ing by two Arabidopsis F box protein: EBF1 and EBF2. Cell 115: 697-689. Rieu, I., C. Mariani, and K. Wetering. 2003. Expression analysis of five tobacco EIN3 family members in relation ti tissue-specific ethylene response. J. Exp. Bot. 54: 2239-2244. Roman, G., B. Lubarsky, J. J. Kieber, M. Rothenbery, and J. R. Ecker. 1995. Genetic analysis of ethylene signal transduction in Arabidopsis thaliana: five novel mutant loci integrated into a stress response pathway. Genetics 139:1393-1409. Rouster, J., L. R. Leah, J. Mundy, and V. C. Mills. 1997. Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expre- ssed in barley grain. Plant J. 11:513-523. Satoh, S., and Y. Esashi. 1982. Effect of α-amynoisobutyric acid and D- and L-amino acids on ethylene production and content 1-aminocyclopropane-1-carboxylic acid in cotyledonary segments of cocklebur seed. Physol. Plant. 54:147-152. Shen, Q., P. Zhang, T. H. Ho. 1996. Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell. 8:1107-1119. Simpson, S. D., K. Nakashima, Y. Narusaka, M. Seki, K. Shinozaki,and K. Yama- guchi-Shinozaki. 2003. Two different novel cis-acting elements of erd1, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence. Plant J. 33:259-270. Solano, R., A. Stepanova, Q. Chao, and J. R. Ecker. 1998. Nuclear events in Ethylene signaling: a transcriptional cascade mediated by ETHYLENE- INSENSITIVE 3 and ETHYLENE-RESPONSE- ACTOR1. Gene Develop. 12:3703-3714. Stange. C., I. Ramírez, I. Gómez, X. Jordana, and L. Holuigue. 1997. Phosphorylation of nuclear proteins directs binding to salicylic acid-responsive elements. Plant J. 11:115-1324. Tieman, D. M., J. A. Ciardi, M. G. Taylor, and H. J. Kale. 2001. Members of the tomato LeEIL (EIN3-like) gene family are functionally redundant and regulate ethylene responses throughout plant development. Plant J. 26:47-58. Ulmasov, T., Z. B. Liu, G. Hagen, and T. J. Guilfoyle. 1995. Composite structure of auxin response elements. Plant Cell 7:1611-1623. Ulmasov, T., J. Murfett, G. Hagen, and T. J. Guilfoyle. 1997. Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements.Plant cell 9:1963-1971. Vogel, J. P., K. E. Woeste, A. Theologis, and J. J. Kieber. 1998. Recessive and dominant mutations in the ethylene biosynthesis gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethyleneoverproduction, respectively. Proc. Natl. Acad. Sci. USA 95:4766-4771. Waki, K., K. Shibuya, T. Yoshioka, T. Hashiba, and S. Satoh. 2001. Cloning of a cDNA encoding EIN3-like protein (Dc-EIL1) and decrease in its mRNA during senescence in carnation flower tissue. J. Exp. Bot. 52:377-379. Wang, C. Y. and D. O. Adams. 1982. Chilling-induced ethylene production in cucumbers (Cucumis sativas L.). Plant Physiol. 69:424-427. Wasternack, C. 2007. Jasmonates: An update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann. Bot. 100: 681–697. William, G. H. 1999. Introduction to Plant Physiology (2nd ed). p.354-356. John Wiley and Sons, Inc. USA. White, A. J., M. A. Dunn, K. Brown, and M. A. Hughes. 1994. Comparative analysis of genomic sequence and expression of a lipid transfer protein gene family in winter barley. J. Exp. Bot. 45:1885-1892. Yamaguchi-Shinozaki, K., and K. Shinozaki. 1993. Arabidopsis DNA encoding two desiccation-responsive rd29 genes. Plant Physiol. 101:1119-1120. Yokotani, N., S. Tamura, R. Nakano, A. Inaba, and Y. Kubo. 2003. Characterization of noval tomato EIN3-like gene (LeEIL4). J. Exp. Bot. 54:2775-2776. Yang, S. F. and N. E. Hoffman. 1984. Ethylene biosynthesis and its regulation in higher plants. Ann. Rev. Plant Physiol. 35:155-189. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41144 | - |
| dc.description.abstract | 為了暸解並調控更年性果實苦瓜之後熟及更年性花卉蝴蝶蘭之花瓣老化，本研究進行乙烯訊息傳導途徑下游之轉錄調控蛋白EIN3/EILs 同源基因之選殖。以苦瓜EIN3同源選殖系pMcEIL56 cDNA 為探針，篩選苦瓜基因組庫，共得到16個基因選殖系。依電泳分離與南方氏雜交分析結果，選取選殖系λMEIL137-2進行次選殖與定序分析。比對pMcEIL56 cDNA序列，λMEIL137-2包含完整之Mc-EIL1基因，序列長3,618 bp，具有2個顯子與1個隱子，並取得5’-flanking序列2,768 bp。其中第一顯子長117 bp，第二顯子長2,355 bp，而第一隱子長1,147 bp，轉譯起始密碼位於第二顯子193 bp處。該基因啟動子具有多種responsive elements，可能受到Auxin、ABA、Jasmonate、逆境等因子共同參與啟動子之調控。
另ㄧ方面，以蝴蝶蘭pPEIL168 cDNA作為探針，篩選蝴蝶蘭基因組庫，共得到14個選殖系。依電泳分離及南方氏雜交分析結果，選取λPEIL 51、53、67等選殖系進行次選殖與基因定序。選殖系λPEIL51與pPEIL177 cDNA 核苷酸1~914 bp序列完全相同，將其基因命名為 Pt-EIL1-1，包含Pt-EIL1-1 基因之5’-flanking 序列3,215 bp、第一顯子166 bp、第一隱子3,223 bp 與第二顯子748 bp，顯子與隱子連接部位符合GT- AG rule，而轉譯起始密碼位於第二顯子98 bp處。選殖系λPEIL 67與pPEIL168 cDNA 核苷酸1~166 bp序列完全相同，將其對應基因命名為 Pt- EIL1-2包含Pt-EIL1 -2基因之 5’-flanking 序列4,104 bp、第一顯子166 bp與第一隱子部分序列199 bp。Pt-EIL1-1與Pt-EIL1-2 基因相對應之5’-flanking region與第一顯子序列相似度為分別為87.6%與99.4%。選殖系λPEIL53與pPEIL177 cDNA核苷酸1,239~2,175 bp序列相似度為99.3 %，共有7個核苷酸相異，導致2個胺基酸不同，推測該基因為PtEIL1-1之等位基因。Pt-EIL1之啟動子亦具有多種responsive elements，可能受到auxin、ABA茉莉酸、乾旱、創傷等因子共同調控啟動子之作用。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-14T17:19:49Z (GMT). No. of bitstreams: 1 ntu-97-R95628108-1.pdf: 1665978 bytes, checksum: 4f9ee9ddc2b2f14b5b1881f0222fa19c (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 中文摘要 1
Summary 3 壹、 前言 5 貳、 前人研究 6 ㄧ、乙烯與苦瓜果實後熟及蝴蝶蘭花瓣老化之關係 6 二、植物體內乙烯生合成路徑與調控 6 三、植物乙烯訊息傳導 7 (一) 乙烯受體基因家族 7 (二) 激酶蛋白 CTR1 8 (三) 膜蛋白 EIN2 9 (四) 核蛋白 EIN3/EILs 9 (五) EIN3 與 ubiquitin / proteosome 蛋白質降解路徑之關係 10 (六) EIN3 結合蛋白 EBF1 / EBF2 11 (七) 核酸外切酶 (exoribonuclease) EIN5 / EIN7 / XRN4 12 四、各物種EIN3/EILs基因之選殖及表現情形 12 (ㄧ) 阿拉伯芥 12 (二) 菸草 14 (三) 番茄 14 (四) 康乃馨 15 (五) 玫瑰 15 (六) 綠豆 16 (七) 苦瓜、蝴蝶蘭、文心蘭與香蕉 16 參、材料方法 19 一、植物材料 19 二、試驗方法 19 (一) 苦瓜與蝴蝶蘭 EIN3 同源基因之選殖 19 (二) 苦瓜與蝴蝶蘭 EIN3 同源基因限制酶圖譜分析 23 (三) 苦瓜與蝴蝶蘭 EIN3 同源基因之次選殖 24 (四) 苦瓜 EIN3 同源基因啟動子表現質體之構築 26 .肆、結果 32 一、苦瓜 EIN3 同源基因結構分析 32 (一) 苦瓜基因組庫之篩選結果 32 (二) 苦瓜 EIN3 同源基因限制酶圖譜分析 32 (三) 苦瓜 EIN3 同源基因序列分析 33 (四) 苦瓜 EIN3 同源基因啟動子序列分析 33 二、蝴蝶蘭基因組庫之篩選 47 (一) 蝴蝶蘭基因組庫之篩選 47 (二) 蝴蝶蘭 EIN3 同源基因限制酶圖譜分析 47 (三) 蝴蝶蘭EIN3 同源基因序列分析 48 (四) 蝴蝶蘭 EIN3 同源基因啟動子序列分析 49 伍、討論 68 ㄧ、苦瓜與蝴蝶蘭EIN3同源基因之結構 68 二、苦瓜與蝴蝶蘭EIN3同源基因之啟動子序列分析 70 陸、結語 73 柒、參考文獻 74 | |
| dc.language.iso | zh-TW | |
| dc.subject | 苦瓜 | zh_TW |
| dc.subject | 蝴蝶蘭 | zh_TW |
| dc.subject | 乙烯訊息傳導 | zh_TW |
| dc.subject | Ethylene Insensitive 3 | en |
| dc.subject | Bitter Gourd | en |
| dc.subject | Phalaenopsis | en |
| dc.title | 苦瓜與蝴蝶蘭EIN3同源基因之選殖與分析 | zh_TW |
| dc.title | Cloning and Analysis of Ethylene Insensitive 3 Homologous Genes from Bitter Gourd and Phalaenopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 黃鵬林 | |
| dc.contributor.oralexamcommittee | 許圳塗,鄭隨和 | |
| dc.subject.keyword | 苦瓜,蝴蝶蘭,乙烯訊息傳導, | zh_TW |
| dc.subject.keyword | Bitter Gourd,Phalaenopsis,Ethylene Insensitive 3, | en |
| dc.relation.page | 78 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-27 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-97-1.pdf 未授權公開取用 | 1.63 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。