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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 張鑫 | |
dc.contributor.author | Yu-Ching Lin | en |
dc.contributor.author | 林育靜 | zh_TW |
dc.date.accessioned | 2021-06-14T17:12:54Z | - |
dc.date.available | 2011-09-11 | |
dc.date.copyright | 2008-09-11 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-25 | |
dc.identifier.citation | Asabe, S. I., Tanji, Y., Satoh, S., Kaneko, T., Kimura, K. & Shimotohno, K. (1997). The N-terminal region of hepatitis C virus-encoded NS5A is important for NS4A-dependent phosphorylation. J Virol 71, 790-796.
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J Viral Hepat 8, 96-102. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41032 | - |
dc.description.abstract | C型肝炎病毒 (Hepatitis C virus, HCV) 非結構性蛋白質NS3具有serine protease和RNA helicase的功能。NS4A作為NS3 protease的輔因子。在NS4A的存在之下,NS3會將病毒的多蛋白質進行切割,同時也會進行NS3的內部切割。在先前的研究中指出,NS3-4A會存在於細胞的粒線體當中,並且利用protease的酵素活性去切割粒線體上的MAVS/IPS-1而去干擾type-I IFN-b 的產生,使宿主的免疫反應受到抑制。近來也有研究顯示,NS4A會累積在細胞粒線體中,進而活化caspase-3及引發細胞凋亡。本研究首先建立能經由doxycycline誘導表現NS3-4A的HepG22-NS3-4A穩定細胞株。在此細胞株觀察到NS3-4A表現時會自行切割得到NS3和NS4A蛋白質,NS3和NS4A在細胞中的分佈大致重疊,位於粒線體和內質網上。過去已知脫氧膽酸會造成粒線體膜電位的改變。在此研究中發現不論是誘導表現NS3-4A的穩定細胞株或在短暫轉染表現NS3-4A的細胞當中,在給予脫氧膽酸的情況下,相較於控制組,都會減緩脫氧膽酸對於粒線體膜電位的影響。當分析293細胞在表現NS3-4A時,對於脫氧膽酸所造成DNA fragmentation、cytochrome c釋放和caspase-3活化的情形,發現NS3-4A的表現能減緩脫氧膽酸所造成cytochrom c的釋放和caspase-3的活化,但是對於脫氧膽酸所造成的DNA fragmentation,則沒有太大的影響。進一步將帶有NS3、NS4A的表現質體分別轉染293細胞,發現NS3和NS4A單獨存在時都具有穩定粒線體膜電位的功能,減緩脫氧膽酸的效應。其中NS4A也能減緩脫氧膽酸所造成的cytochrome c釋放。已知位於粒線體的p53會促使細胞進行粒線體相關細胞凋亡。實驗亦發現,當NS3-4A表現時,p53在粒線體上的分佈量明顯下降。NS3-4A或許藉由調控粒線體上的p53,來達到抗細胞凋亡的效果。但其真正作用機制還有待進一步的確認。 | zh_TW |
dc.description.abstract | Hepatitis C virus (HCV) NS3 is a nonstructural protein that possesses protease and RNA helicase activity. NS4A is a cofactor of NS3 protease, it promotes HCV polyprotein processing and NS3 internal cleavage. NS3-4A can localize at mitochondria to cleave the mitochondria protein MAVS/IPS-1 then to block the type-I IFN-b pathway. NS4A was also found to accumulate on mitochondria membrane and induce mitochondria-mediated apoptosis. In this study, HepG22-NS3-4A inducible cell line was established. Expression of NS3-4A protein in the stable line resulted in a cleavage of NS3-4A protein and partial colocalization of the NS3 and NS4A to the mitochondria. Expression of NS3-4A also restored the mitochondria membrane potential collapsed by DCA both in the inducible HepG22-NS3-4A cell line and in 293 transient transfection system. Both cytochrome c release and caspase-3 activity induced by DCA can be repressed by NS3-4A, but NS3-4A can not protect the DCA-induced DNA fragmentation. Further dissection indicated that NS3 or NS4A alone can restore the mitochondria membrane potential collapse by DCA. NS4A can decrease the DCA-induced cytochrome c release. The mechanisms involved in the NS3-4A mediated anti-apoptotic effects are not completely understood. Nevertheless, it was reported that mitochondria p53 can promote mitochondria-mediated apoptosis. The observation that the level of p53 at mitochondrial fraction was lower in the NS3-4A expressing cells than that of the control cells, suggesting that p53 maybe a target of the NS3-4A regulated anti-apoptotic effect. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T17:12:54Z (GMT). No. of bitstreams: 1 ntu-97-R95445114-1.pdf: 2020304 bytes, checksum: 3e605c16e097f43e17df79bd6d6fd9b8 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 口試委員會審定書 i
摘要 ii 英文摘要 iii 目錄 iv 圖目錄 vii 緒論 1 一、C型肝炎的歷史 1 二、C型肝炎病毒的基因體結構及功能 1 三、C型肝炎病毒非結構性蛋白質NS3的特性 4 四、C型肝炎病毒非結構性蛋白質NS4A的特性 6 五、抑癌蛋白p53 8 六、細胞凋亡 10 研究方向 11 材料與方法 12 一、藥品 12 二、酵素 13 三、染劑 13 四、抗體 13 五、細胞培養液及轉染試劑 13 六、套組試劑 14 七、細胞株 14 八、實驗室提供的質體 15 九、質體的構築 17 十、DNA轉染 17 十一、流式細胞儀分析 18 十二、蛋白質定量 18 十三、正十二烷硫酸納-聚炳醯胺板電泳 19 十四、西方墨點法 20 十五、分離粒線體 21 十六、免疫螢光染色 21 十七、去氧核醣核酸斷裂分析 22 十八、caspase-3 活性的測定 22 結果 23 一、建立HepG22-NS3-4A穩定表現細胞株 23 二、NS3-4A 蛋白質在細胞中的分佈情形 23 三、NS3-4A對粒線體胞膜電位的影響 24 四、NS3-4A對於cytochrome c釋放的影響 25 五、NS3-4A對於宿主細胞caspase-3活性的影響 26 六、NS3-4A對於DNA fragmentation的影響 26 七、分別檢視NS3和NS4A對於粒線體膜電位的影響 26 八、NS4A對於cytochrome c釋放的影響 27 九、NS3-4A對於粒線體上p53的影響 27 討論 29 一、NS3-4A在細胞當中的分佈情形 29 二、NS3-4A對於粒線體相關細胞凋亡的影響 29 圖表 33 參考文獻 45 | |
dc.language.iso | zh-TW | |
dc.title | C型肝炎病毒非結構性蛋白質NS3-4A對於宿主細胞之細胞凋亡的調節作用 | zh_TW |
dc.title | Hepatitis C virus NS3-4A protein regulates apoptosis of host cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 康照洲,林素芳,董馨蓮 | |
dc.subject.keyword | C型肝炎病毒,非結構性蛋白質,粒線體,細胞凋亡, | zh_TW |
dc.subject.keyword | HCV,NS3-4A,mitochondria,apoptosis, | en |
dc.relation.page | 51 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-28 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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