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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 胡凱康(Kae-Kang Hwu) | |
dc.contributor.author | Hshin-Ping Wu | en |
dc.contributor.author | 吳心平 | zh_TW |
dc.date.accessioned | 2021-06-14T17:05:51Z | - |
dc.date.available | 2008-08-06 | |
dc.date.copyright | 2008-08-06 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-27 | |
dc.identifier.citation | 行政院農業委員會 (2006) 農業統計年報。2008年7月14日取自http://www.coa.gov.tw/view.php?catid=195
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40901 | - |
dc.description.abstract | 生殖障礙在物種形成和物種分化的過程中扮演了一個非常重要的角色。研究生殖障礙的方法之一便是分析偏離期望孟德爾分離率的不平衡分離,而研究不平衡分離最好的方法就是發展高密度的分子連鎖圖譜,利用DNA分子標記對整個基因體做一次全面性的掃描,再藉由模式估算不平衡分離基因座在染色體上所發生的位置與效應,以及其偏離的方向。
建立以秈稻台中秈10號為輪迴親、稉稻台稉2號為貢獻親的秈稉稻雜交BC1F1族群,利用133個插入-缺失 (insertion-deletion, InDel) 分子標記與14個簡單序列重複 (simple sequence repeat, SSR) 分子標記對254棵植株進行基因型定型分析、繪製出總長共1494.7cM、平均距離為11.072cM的連鎖圖譜;相較於原先的連鎖圖譜,在某些區域上分子標記間的遺傳距離呈現大量的偏差。不平衡分離發生在第1、2、3、6、10、11、12條染色體上,其中第3、6、11條染色體上發生不平衡分離的分子標記有「群集」的現象,且三者都傾向於秈稻同質結合型。比較兩分子標記間遺傳距離偏差嚴重的位置與發生不平衡分離群集的位置有重疊,與其他提出當不平衡分離的分子標記偏向同方向時會低估遺傳距離的研究一致。 利用卡方檢定檢測試驗族群中不同染色體上發生不平衡分離的分子標記兩兩之間並沒有相互的交感作用,因此決定使用單一不平衡分離基因座 (segregation distortion locus, SDL) 的模式,也就是以最大概似法 (EM演算法) 估算單一不平衡分離基因座在染色體上的位置與其效應,總計在該水稻雜交組合12條染色體上共有10個SDL。 | zh_TW |
dc.description.abstract | Reproductive barriers are thought to play an important role in the processes of speciation and differentiation. One of the applications of DNA markers in studying reproductive barrier is the analysis of segregation distortions that deviates from expected Mendelian segregation ratios. Therefore, it is necessary to develop high-density molecular linkage maps and to analyze the proportions of genotypes over the whole genome, and moreover, to estimate the locations and effects of segregation distortion loci and the directions of the deviation of genotype frequencies by using model methods.
The BC1F1 population consisting of 254 individuals was created from a backcross between an indica variety Taichung Sen10 as recurrent parent and a japonica variety Taikeng2 as donor parent. Genotyping was performed using 133 insertion-deletion (InDel) markers and 14 simple sequence repeat (SSR) markers, yielding a genetic map of 1494.7 cM, with an average interval of 11.072 cM. However, in some regions the map distances between markers showed considerable deviation from the published linkage map. Segregation distortions were found on chromosome 1, 2, 3, 6, 10, 11 and 12 respectively. There were some distorted markers clustered in special regions on chromosome 3, 6 and 11, and they all deviated toward indica homozygote. The positions of regions with serious biased genetic distances and regions where distorted markers clustered were overlapping. This result conforms with other studies which showed that the genetic distances between markers distorted toward the same direction will be underestimated. Chi-square test revealed that there is no interaction between distorted markers on different chromosomes, hence single SDL model method is used. Maximum likelihood method is implemented via an Expectation-Maximum algorithm to estimate the locations and effects of segregation distortion loci. Totally, there are 10 SDLs on all chromosomes in this backcross population from a cross between TCS10 and TK2. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T17:05:51Z (GMT). No. of bitstreams: 1 ntu-97-R95621118-1.pdf: 1593850 bytes, checksum: 986927d7119d75cac06fd3d0c45e7356 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 內容目錄
口試委員會審定書 i 致謝 ii 中文摘要 iii Abstract iv 內容目錄 vi 圖片目錄 vii 表格目錄 viii 壹、 前言 1 貳、 前人研究 3 參、 材料與方法 11 一、 試驗族群 11 二、 水稻葉片DNA萃取與定量 13 三、 InDel分子標記與SSR分子標記的開發 15 四、 基因型定型分析 (Genotyping) 18 五、 連鎖圖譜的建立、不平衡分離的分析與分子標記兩兩之間的交感作用 20 六、 以最大概似法估算不平衡分離基因座的位置與其效應 22 肆、 結果 28 一、 分子標記與連鎖群圖譜 28 二、 不平衡分離的現象 42 三、 分子標記兩兩之間的交感作用 50 四、 不平衡分離基因座 (SDL) 的位置與其效應 57 伍、 討論 61 一、 分子標記的使用 61 二、 連鎖群圖譜與不平衡分離的現象 63 三、 分子標記兩兩之間的交感作用 65 四、 不平衡分離基因座的位置與其效應 67 五、 試驗的設計 69 陸、 參考文獻 72 柒、 附錄 80 一、Hedrick與Muona最大概似法模式配合Cheng等人EM演算法估算過程 80 二、三個分子標記的兩種模式(L與R)估算最大概似法(EM演算法)SAS程式 90 圖片目錄 圖一 水稻TSC10/TK2//TCS10回交族群分子連鎖圖譜 36 圖二 12條染色體上秈型對偶基因頻度趨勢圖 44 圖三 12條染色體上分子標記兩兩之間的交感作用 52 圖四 第1條染色體與第2、3、4、8條染色體之間的交感作用 53 表格目錄 表一 147組分子標記於水稻12條染色體上的物理圗譜位置與遺傳位置 30 表二 兩種雜交組合分子標記間遺傳距離的比較 33 表三 試驗族群中發生不平衡分離的分子標記與其卡方適合度檢定的結果 43 表四 以最大概似法(EM演算法)估算不平衡分離基因座位置與其效應的結果 60 | |
dc.language.iso | zh-TW | |
dc.title | 秈稉稻雜交BC1F1族群之不平衡分離 | zh_TW |
dc.title | Segregation distortion in the BC1F1 population from a cross between indica and japonica rice. | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝兆樞(Jaw-Shu Hsieh),林彥蓉(Yann-Rong Lin) | |
dc.subject.keyword | 水稻,回交族群,不平衡分離,最大概似法,EM演算法, | zh_TW |
dc.subject.keyword | Oryza sativa L.,BC1F1 population,segregation distortion,maximum likelihood method,expectation-maximum algorithm, | en |
dc.relation.page | 78 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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