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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李英周 | |
| dc.contributor.author | Yung-Cheng Chang | en |
| dc.contributor.author | 張詠政 | zh_TW |
| dc.date.accessioned | 2021-06-14T17:01:18Z | - |
| dc.date.available | 2011-08-22 | |
| dc.date.copyright | 2011-08-22 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-11 | |
| dc.identifier.citation | Andersen, R.A. 2005. Algal culturing techniques. Elsevier Academic Press, London, UK, 596pp.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40809 | - |
| dc.description.abstract | 傳統繼代保存藻類操作方法簡便與快速，但在繼代過程中可能產生汙染與遺傳漂變，使藻種汙染或缺失。而超低溫保存是將物種保存在-196℃的液態氮中，理論上可永久保存物種，也可避免上述繼代保存的缺點，已被廣泛應用於藻類種原保存。本研究探討六種常應用於台灣水產養殖魚蝦貝苗的海洋微藻：擬球藻(Nannochloropsis oculata)，扁藻(Tetraselmis sp.)，簡單雙眉藻(Amphora exigua)，半舟行藻(Seminavis gracilenta)，骨藻(Skeletonema costatum)及等鞭金藻(Isochrysis galbana)的最佳超低溫保存條件。探討5種常用的滲透性抗凍劑，二甲基亞碸（dimethyl sulfoxide；DMSO）、甲醇（methanol；MeOH）、甘油(glycerol；GLY)、乙二醇（ethylene glycol；EG）及丙二醇（propylene glycol；PG）和5種不同濃度(0%，5%，10%，15%，20%)，自室溫(26℃)，以每分鐘-1℃降至-40℃，再放入液態氮中保存一天後，經40℃水浴解凍。檢測解凍恢復後，藻類是否恢復生長，營養成份與DNA完整性及其最佳處理組合。結果顯示，除了骨藻無法超低溫保存外，其餘五藻種最高活存率的抗凍劑與濃度處理組合均不一樣。擬球藻以20%乙二醇 (89%)，扁藻以20%丙二醇 (57%)，簡單雙眉藻以20%二甲基亞碸 (90%)，半舟行藻以20%二甲基亞碸(94%)及等鞭金藻以15%甘油 (72%)，有最高的活存率。本研究證實藻類在超低溫保存前後其營養成份(粗蛋白、粗脂肪、水份、灰份、不含氮抽出物)與遺傳穩定性沒有顯著差異。 | zh_TW |
| dc.description.abstract | Cryopreservation at ultra-low (-196℃ in liquid nitrogen) has many advantages over the conventional maintenance of algae strains using serial subculture, it is becoming the method of choice for the long-term conservation of algae nowadays. This study determining the optimal protocol for cryopreservation of six marine microalgae : Nannochloropsis oculata, Tetraselmis sp., Seminavis gracilenta, Amphora exigua, Skeletonema costatum and Isochrysis galbana using the two-step method protocol with five cryoprotectant and five consentration are discussed. The results showed the best cryoprotectant for algae is species-specific. The highest post-thawed viability for N. oculata was 89% with 20% ethylene glycol(EG), for T. elliptica was 57% with 20% propylene glycol(PG), for S. gracilenta was 94% with 20% dimethyl sulfoxide(DMSO), for A. exigua was 90% with 20% dimethyl sulfoxide(DMSO) and for I. galbana was 72% with 15% glycerol(GLY). Two-step method protocol was not suitable for cryo-storage of Skeletonema costatum. The results showed that the nutritional composition of the microalgae were not significantly affected after cryopreservation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T17:01:18Z (GMT). No. of bitstreams: 1 ntu-100-R98b45005-1.pdf: 1385430 bytes, checksum: b47a9cd15efefcc56c740515dac1fbb9 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 目錄…………………………………………………………………I
表目錄……………………………………………………………III 圖目錄……………………………………………………………IV 中文摘要……………………………………………………………V 英文摘要……………………………………………………………VI 第一章 前言…………………………………………………………1 (一) 藻類應用概述……………………………………………1 (二) 藻類種原保存……………………………………………3 (三) 藻類超低溫保存研究概況………………………………5 (四) 影響藻類凍結保存活存率的因子………………………8 (五) 藻類超低溫保存後之遺傳穩定性與營養成份…………12 (六) 研究動機與目的…………………………………………13 第二章 材料與方法…………………………………………………14 (一) 實驗材料…………………………………………………14 (二) 抗凍劑毒性測試…………………………………………15 (三) 兩步法超低溫保存(-196℃)測試………………………15 (四) 藻類活存判別與活存率計算……………………………16 (五) 營養成份分析……………………………………………16 (六) 遺傳穩定性分析…………………………………………18 (七) 統計分析…………………………………………………18 第三章 結果…………………………………………………………19 (一) 抗凍劑毒性測試…………………………………………19 (二) 兩步法超低溫保存(-196℃)測試………………………19 (三) 營養成份分析……………………………………………20 (四) 遺傳穩定性分析…………………………………………21 第四章 討論…………………………………………………………22 第五章 結論…………………………………………………………27 第六章 參考文獻……………………………………………………28 | |
| dc.language.iso | zh-TW | |
| dc.title | 六種台灣水產養殖常用的海洋微細藻類之兩步法超低溫保存的研究 | zh_TW |
| dc.title | Study in Cryopreservation of Six Marine Microalgae for Aquaculture by two-step protocol | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 郭金泉 | |
| dc.contributor.oralexamcommittee | 陳衍昌,蘇惠美 | |
| dc.subject.keyword | 超低溫保存,抗凍劑,海洋微藻,營養成份, | zh_TW |
| dc.subject.keyword | cryopreservation,cryoprotectant,marine microalgae,nutritional composition, | en |
| dc.relation.page | 60 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-12 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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