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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 湯森林(Sen-Lin Tang) | |
| dc.contributor.author | Cheng-Yu Yang | en |
| dc.contributor.author | 楊承諭 | zh_TW |
| dc.date.accessioned | 2021-06-14T16:51:09Z | - |
| dc.date.available | 2014-08-18 | |
| dc.date.copyright | 2011-08-18 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-12 | |
| dc.identifier.citation | 參考文獻：
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40554 | - |
| dc.description.abstract | 近年來台灣綠島地區的珊瑚大量地由平鋪狀星野黑皮海綿（以下簡稱黑皮海綿）覆蓋而死亡，稱為“珊瑚黑病”。野外觀察發現黑皮海綿的前、後端於外觀上有差異，而這些差異是前人研究中沒有被探討。因此，本研究針對綠島的黑皮海綿，採取前端、後端樣本分別進行電子顯微鏡的觀察及轉錄體的定序分析。另外建立一組與珊瑚隔離生長的解剖盒樣本，比較三個不同生長位置的黑皮海綿，討論他們在生理生化與外觀上差異之間的關係。電子顯微鏡觀察結果發現，黑皮海綿樣本體內含有大量的球狀藍綠菌及骨針，前端樣本的骨針呈隨機排列，而解剖盒海綿樣本則常呈束狀排列。此外，前端樣本的細胞狀況較後端樣本完整且良好。轉錄體的分析結果顯示，前端樣本較後端及解剖盒樣本表現較多與能量相關的基因，這些能量可能是黑皮海綿覆蓋珊瑚後掠食得到的，可以用來增殖與擴張生長空間。後端樣本則表現較多與細胞凋亡相關反應及DNA修復的基因，說明此部分的黑皮海綿正處於生理狀況較差或逆境的狀態。解剖盒樣本所表現的基因大多與細胞移動有關，與其絲狀結構及束狀排列的骨針相呼應。由於黑皮海綿細胞內含有大量的原核生物，待原核生物的轉錄體定序完成之後，將能經由互相比較黑皮海綿及內含的原核生物轉錄體的表現，期望能找出黑皮海綿與體內原核生物之間的相關的生理代謝途徑並推測黑皮海綿與內含的原核生物之間的關係，和黑皮海綿與珊瑚之間的交互作用。 | zh_TW |
| dc.description.abstract | Recently, the corals in Lyudao, Taiwan, are prevalently suffered from the attack of “Black Disease”, caused by the overlaying of an encrusting sponge T. hoshinota. To understand the insights underlying T. hoshinota’s behavior, we sampled different parts of the sponge in Lyudao, including front-end, middle-site, and an isolated culture in mesh box as a control, for comparative purpose. The morphology of T. hoshinota was studied using electron microscopes, and the transcriptome profile was analyzed for validating possible associations between gene expression and appearance. The electron microscopes observation indicated that there were many coccus-like cyanobacteria, and tylostyle spicules were arranged regularly in the mesh box sample but disorderly in the front-end sample. Also, the front-end sample was in a healthier condition as being more intact compared to other samples. According to the transcriptomic analysis, the front-end sample had higher expression of the energy-related genes, suggesting that the front T. hoshinota might acquire energy by using coral as “food resource” for proliferation and territory expansion. The middle-site sample expressed more transcripts related to apoptosis and DNA repair, saying that this part of the sponge might be under stress. The preferred expression on cell motility genes in the mesh box sample revealed possible purpose of its silk-like appearance and might lead T. hoshinota to move. This transcriptomic study is just a start uncovering physiological and biochemical features of T. hoshinota. Further understandings about the interactions between coral, sponge, and associated bacteria can be obtained when the microbial gene expression data were incorporated. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T16:51:09Z (GMT). No. of bitstreams: 1 ntu-100-R98241218-1.pdf: 7712013 bytes, checksum: 830b4da6157c79b77ef1ded507938d78 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 誌謝 i
摘要 ii Abstract iii 中英文對照表 iv 表目錄 xiii 圖目錄 xiv 一、 研究背景 1 1.1. 海洋中的海綿 1 1.1.1. 海綿的特徵 1 1.1.2. 海綿的生殖行為 2 1.1.3. 海綿的分布 2 1.1.4. 海綿內與微生物的共生現象 3 1.1.5. 富藍綠菌海綿（cyanobacteriosponge） 4 1.1.6. 常見的海綿共生藍綠菌 5 1.2. 星野黑皮海綿 （Terpios hoshinota）介紹 6 1.2.1. 歷史及生態問題 6 1.2.2. 習性及構造 7 1.2.3. 內棲微生物 8 1.3. 研究動機與目的 8 二、 材料與方法 10 2.1 採樣地點及採樣方法 10 2.2 樣本的保存 10 2.3 電子顯微鏡觀察 11 2.4 全RNA萃取 12 2.5 RNA品質檢定 12 2.6 RNA組成 12 2.7 RNA樣本中真核與原核生物的比例 13 2.8 原核生物（藍綠菌為主）mRNA純化 13 2.9 次世代解序技術定序 15 2.10 定序品質檢定 16 2.11 序列組合 16 2.12 以KAAS進行KEGG資料庫比對 17 2.13 標準化 17 2.14 相對表現量熱圖 18 2.15 相對表現量三方比較圖 18 2.16 主要基因相對表現量差異比較 19 三、 結果 21 3.1. 比較不同部位黑皮海綿的顯微結構 21 3.1.1. SEM觀察 21 3.1.2. TEM觀察 21 3.2. 轉錄體定序樣本製備 22 3.2.1. 黑皮海綿樣本內RNA的組成 22 3.2.2. 黑皮海綿定序樣本RNA品質檢定 23 3.2.3. 黑皮海綿全RNA中，真核與原核RNA的比例 23 3.2.4. 原核生物（藍綠菌為主）定序樣本mRNA純化 24 3.3. 轉錄體定序結果 24 3.3.1. 定序結果與序列品質 24 3.3.2. 序列組合 25 3.3.3. 蛋白質功能及表現量標準化 25 3.4. 基因相對表現量比較結果 26 3.4.1. 基因比較熱圖及三方比較圖 26 3.4.2. 醣解作用及糖質新生作用 27 3.4.3. 脂肪酸的代謝與生成路徑 27 3.4.4. 胺基酸代謝與生成 28 3.4.5. 呼吸作用 28 3.4.6. 細胞移動 29 3.1.1. 細胞凋亡 30 3.4.7. 聚合酶 31 3.4.8. DNA修復功能蛋白 31 四、 討論與結論 32 4.1 電子顯微鏡觀察 32 4.1.1 SEM觀察 32 4.1.2 TEM觀察 33 4.2 原核生物mRNA純化 34 4.3 轉錄體生物資訊分析結果 35 4.4 代謝相關基因之相對表現量差異 35 4.5 移動相關基因之相對表現量差異 36 4.6 細胞凋亡相關基因之相對表現量差異 38 4.7 原核mRNA汙染問題 39 4.8 未來目標 40 4.9 結論 41 參考文獻 43 | |
| dc.language.iso | zh-TW | |
| dc.title | 星野黑皮海綿轉錄體之分析 | zh_TW |
| dc.title | Transcriptome analysis of Terpios hoshinota | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 謝文陽(Wung-Yang Shieh) | |
| dc.contributor.oralexamcommittee | 戴昌鳳(Chang-Feng Dai),劉秀美(Shiu-Mei Liu) | |
| dc.subject.keyword | 星野黑皮海綿,黑皮海綿,藍綠菌,珊瑚黑病,電子顯微鏡,轉錄體定序,生理代謝途徑,細胞凋亡,細胞移動, | zh_TW |
| dc.subject.keyword | T. hoshinota,cyanobacteria,coral,transcriptome,black disease,metabolism pathway,apoptosis,cell motility, | en |
| dc.relation.page | 103 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-12 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| 顯示於系所單位： | 海洋研究所 | |
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