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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 賈景山,郭生興 | |
| dc.contributor.author | Tuan-Yu Kao | en |
| dc.contributor.author | 高端佑 | zh_TW |
| dc.date.accessioned | 2021-06-14T16:48:45Z | - |
| dc.date.available | 2017-10-01 | |
| dc.date.copyright | 2011-10-03 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-12 | |
| dc.identifier.citation | 1. Nanci A: , Ten Cate's Oral Histology Development, Structure, and Function 7th edition, Mosby Elsevier 319~357
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40476 | - |
| dc.description.abstract | 放射線照射引發口腔黏膜潰瘍是頭頸部癌症放射線治療中常見的副作用，但目前仍無有效的治療藥劑。臨床上的治療方法如給予含有麻醉劑的漱口藥水、維持好的口腔衛生、營養支持、感染控制、及給予角質細胞生長因子或輻射保護劑等支持性照護，上述方法雖然臨床上可達到部份的控制，然而卻無法經濟有效地以及於分子層級有針對性地減輕口腔黏膜潰瘍。發炎前驅物活性氧分子ROS及細胞激素IL-1β、TNF-α常見於口腔粘膜潰瘍組織中，證明口腔黏膜潰瘍是一種發炎相關疾病。牛樟芝Antrodia camphorata是台灣特有的傳統中草藥，具有抗發炎、抗氧化和免疫調節的功能。本實驗將建立小鼠吻部局部照射放射線單一劑量37.5 Gy所引發的口腔黏膜潰瘍模型，並以舌背黏膜為觀查對象，作為評估放射線引發口腔黏膜炎之臨床疾病理變化，以及觀察牛樟芝抗發炎作用緩解口腔潰瘍的有效性。實驗於放射線照射前五天起開始分別餵食水以及牛樟芝，並於照射後第0、3、6、9、12天分批犧牲小鼠，取其舌背黏膜作病理組織分析上皮厚度及肥大細胞，以及前發炎細胞激素基因的表現量偵測。實驗結果顯示，兩組小鼠體重於照射放射線後開始下降，但兩組無顯著差異；兩組舌背黏膜厚度普遍上都由舌根往舌尖方向依序遞減之現象，餵食牛樟芝組的平均舌背黏膜及舌頭各部位皆有比餵食水的組別更厚的趨勢；此外牛樟芝組亦有抑制肥大細胞數目增生或聚集的作用；發炎相關細胞激素基因表現的差異則不明顯；另外根據本實驗結果建立臨床小鼠之放射線引發口腔黏膜炎之分級方法。總結，本研究初步建立放射線治療引發口腔潰瘍之小鼠模型，此外餵食牛樟芝有緩和小鼠口腔黏膜炎的趨勢。 | zh_TW |
| dc.description.abstract | Radiation-induced mucositis (RIM) is a common side effect of radiation therapy in head and neck cancer patients. So far, management has been directed to to supportive cares such as mouth rinse with anesthetics, nutritional support, infection treatment, or systemic administration of keratinocyte growth factor or radioprotectors. Whereas these interventions have been valuable for clinical management, they have not been efficiently or collectively directed to molecularly targeted prevention and treatment. RIM is an inflammation-associated disease, because reactive oxygen species and proinflammatory cytokine such as IL-1β and TNF-α were detected in mucositis tissue. Antrodia camphorata (AC) is a typical Chinese herb found only in Taiwan, and it has anti-inflammation, antioxidant and immunomodulatory properties. This study established a RIM mice model via local irradiation of single dose—37.5 Gy toward snout and observes the dorsal surface mucosa of tongue for evaluating the clinical and histopathological changes. Water or AC was feed from day 5 before iiradiation. After irradiated on day 0, each group of mice were sacrificed on day 0, 3, 6, 9, 12, and the tongue were incised for histopathological analysis of epithelial thickness, mast cells counts and pro-inflammatory cytokine gene expression. The body weight began to decline after radiation exposure, but there were no significant differences; epithelial thickness of dorsal surface mucosa revealed gradually descending amount of thickness from tongue posterior toward tip in both groups; AC group showed generalized thicker than the water group. In addition, AC group seemed to have the effect that inhibits mast cell hyperplasia or recruitment. Gene expression profiles of pro-inflammation cytokine showed no significant difference. We also established the grading system of dorsal surface mucosa in RIM mice model. In conclusion, the radiation induced mucositis mouse model was established and we founded that AC would ameliorate RIM in mice. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T16:48:45Z (GMT). No. of bitstreams: 1 ntu-100-R98422029-1.pdf: 253240613 bytes, checksum: 4593df919df2ebe60256db4376d21a70 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌 謝 iii 中文摘要 v ABSTRACT vii 目 錄 ix 圖目錄 xiii 表目錄 xv 附錄目錄 xvi 第1章 導論及文獻回顧 1 1.1 本章概要 1 1.2 口腔黏膜簡介 1 1.2.1 口腔黏膜之定義 1 1.2.2 口腔黏膜之增生與成熟 1 1.2.3 口腔黏膜之解剖構造 2 1.2.4 固有層之結構 2 1.2.5 小鼠舌背構造之簡介 2 1.3 口腔黏膜炎簡介 3 1.3.1 口腔黏膜炎發生機制之研究回顧 3 1.3.2 口腔黏膜炎發生之五階段模型 4 1.3.3 口腔黏膜炎之危險因子 8 1.3.4 口腔環境與口腔黏膜炎之關係 10 1.3.5 口腔黏膜炎之治療指引及未來研究方向 11 1.4 放射線引發黏膜炎於動物模型之回顧 12 1.5 牛樟芝簡介 13 1.5.1 命名及分類地位 13 1.5.2 牛樟芝型態特性 14 1.5.3 牛樟芝一般成份分析 15 1.5.4 生物功能活性研究 15 1.6 肥大細胞與放射線引發口腔黏膜炎之關係 19 1.6.1 肥大細胞簡介 19 1.6.2 肥大細胞與放射線引發口腔黏膜炎關係之回顧 20 1.6.3 肥大細胞與放射線引發腸道黏膜炎及肺炎關係之回顧 20 1.7 本實驗之研究動機 21 1.8 本實驗之研究主旨及目標 21 第2章 實驗材料與方法 23 2.1 本章概要 23 2.2 實驗動物 23 2.3 牛樟芝製備 23 2.3.1 牛樟芝來源 23 2.3.2 牛樟芝懸浮液製備 23 2.4 照射放射線 24 2.4.1 照射放射線之設備 24 2.4.2 照射放射線之方法 24 2.5 實驗用麻醉藥 25 2.5.1 若朋 25 2.5.2 舒泰 50 25 2.5.3 麻醉劑調配方式 25 2.6 實驗分組及流程 26 2.6.1 分組方式 26 2.6.2 實驗流程 26 2.7 檢體採集 26 2.7.1 檢體採集方式 26 2.7.2 檢體之固定與包埋 27 2.8 分析口腔黏膜炎之方法 27 2.8.1 觀察口腔黏膜炎之方法 27 2.8.2 切片拍照及分段方式 28 2.8.3 上皮層厚度之測量 28 2.9 組織染色 29 2.9.1 蘇木紫-伊紅染色 29 2.9.2 甲苯胺藍染色 29 2.10 訊息核糖核酸之抽取、反轉錄與定量 30 2.10.1 訊息核糖核酸之抽取與反轉 30 2.10.2 定量即時聚合酶連鎖反應 31 2.11 統計方法 31 第3章 實驗結果 33 3.1 本章概要 33 3.2 小鼠體重之變化趨勢 33 3.3 小鼠舌頭背側面之黏膜變化 34 3.4 小鼠舌背H&E染色切片之觀察比較 35 3.5 舌背黏膜厚度之變化趨勢 37 3.5.1 Vehicle組與AC組之厚度變化 37 3.5.2 Tongue posterior之厚度變化與比較 37 3.5.3 Tongue middle之厚度變化與比較 37 3.5.4 Tongue tip之厚度變化與比較 38 3.5.5 平均厚度之變化與比較 38 3.6 小鼠舌背toluidine blue染色切片之觀察比較 38 3.7 肥大細胞之變化趨勢 39 3.7.1 Vehicle組與AC組之變化 39 3.7.2 Tongue posterior之比較 39 3.7.3 Tongue middle之比較 40 3.7.4 Tongue tip之比較 40 3.7.5 肥大細胞總數之變化與比較 40 3.8 前發炎性細胞激素相關基因表現量 41 第4章 討論 43 4.1 本章概要 43 4.2 放射線引發口腔黏膜炎之舌黏膜外觀變化探討以及餵食牛樟芝之比較 43 4.2.1 分析方法之探討 43 4.2.2 實驗結果之探討 44 4.3 放射線引發口腔黏膜上皮層厚度變化以及餵食牛樟芝之比較 45 4.3.1 分析方法之探討 45 4.3.2 實驗結果之探討 47 4.4 肥大細胞於放射線引發口腔黏膜炎之探討以及餵食牛樟芝之比較 50 4.4.1 數量變化之探討 50 4.4.2 功能作用之探討 52 4.5 放射線引發口腔黏膜炎之發炎相關基因表現變化及餵食牛樟芝之比較 53 4.6 探討放射線引發口腔黏膜炎於小鼠模型之分級建立 54 參考文獻 55 圖 61 表 103 附錄 107 | |
| dc.language.iso | zh-TW | |
| dc.subject | 放射線 | zh_TW |
| dc.subject | 口腔黏膜炎 | zh_TW |
| dc.subject | 小鼠模型 | zh_TW |
| dc.subject | mouse model | en |
| dc.subject | radiation | en |
| dc.subject | oral mucositis | en |
| dc.title | 建立放射線引發口腔潰瘍之小鼠模型及組織病理之分析 | zh_TW |
| dc.title | Establishment and histopathological analysis of radiation induced mucositis mouse model | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 成佳憲,江俊斌 | |
| dc.subject.keyword | 口腔黏膜炎,小鼠模型,放射線, | zh_TW |
| dc.subject.keyword | oral mucositis,mouse model,radiation, | en |
| dc.relation.page | 123 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-12 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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