請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40368完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳媺玫 | |
| dc.contributor.author | Chin-Ju Weng | en |
| dc.contributor.author | 翁錦如 | zh_TW |
| dc.date.accessioned | 2021-06-14T16:45:52Z | - |
| dc.date.available | 2013-08-04 | |
| dc.date.copyright | 2008-08-04 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-30 | |
| dc.identifier.citation | 林鈺倩、陳媺玫。魚類來源乳酸鏈球菌胞外產物之促細胞增生物質特性分析。台灣獸醫誌 33：135-143。2007
徐達。錦鯉疱疹病毒傳播途徑之探討。國立台灣大學獸醫學研究所碩士論文。2007 徐達、涂堅、鄭穹翔、陳媺玫。錦鯉疱疹病毒傳播途徑之探討。台灣獸醫誌33 ：88-95。2007 Amend DF & McDowell T. Comparison of various procedures to detect neutralizing antibody to the channel catfish virus in California brood channel catfish. Prog fish-cult：6-12, 1984. Antychowicz J, Reichert M, Matras M, Bergmann SM & Haenen O. Epidemiology, pathogenicity and molecular biology of koi herpesvirus isolated in Poland. Bull Vet Inst Pulawy 49：367-373, 2005. Aoki T, Hirono I, Kurokawa K, Fukuda H, Nahary R, Eldar A, Davison AJ, Waltzek TB, Bercovier H & Hedrick RP. Genome sequences of three koi herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide. J Virol 81：5058-5065, 2007. Barrow AD, Burgess SC, Baigent SJ, Howes K & Nair VK. Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus. J Gen Virol 84：2635-2645, 2003. Bercovier H, Fishman Y, Nahary R, Sinai S, Zlotkin A, Eyngor M, Gilad O, Eldar A & Hedrick RP. Cloing of the koi Herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiol dnlm 5：13, 2005. Bergmann SM, Kempter J, Sadowski J & Fichtner D. First detection, confirmation and isolation of koi herpesvirus (KHV) in cultured common carp (Cyprinus carpio L.) in Poland. Bull Eur Assoc Fish Pathol 26：97-104, 2006. Bowser PR, Munson AD, Jarboe HH, Francis-Floyd R & Waterstrat PR. Isolation of channel catfish virus from channel catfish, Ictalurus punctatus (Rafinesque), broodstock. J fish dis 8：557-561, 1985. Brautigam AR, Dutko FJ, Olding LB & Oldstone MB. Pathogenesis of Murine Cytomegalovirus Infection: the M acrophage as a Permissive Cell for Cytomegalovirus Infection, Replication and Latency. J gen Virol 44：349-359, 1979. Bretringer A, Fische-Scherl T, Oumouna M, Hoffmann R & Truyen U. Mass mortalities in koi carp, Cyprinus carpio, associated with gill and skin disease. Bull Eur Assoc Fish Pathol 19：182–185, 1999. Chilmonczyk S, Voccia I & Monge D. Pathogenesis of viral haemorrhagic septicaemia virus: cellular aspects. Vet. Res 26：505-511, 1995. Chinchar VG, Rycyzyn M, Clem LW & Miller NW. Productive infection of continuous lines of channel catfish leukocytes by channel catfish virus. Virology 193： 989-992, 1993. Dannevig BH, Falk K & Namork E. Isolation of the causal virus of infectious salmon anaemia (ISA) in a long-term cell line from Atlantic salmon head kidney. J Gen Virol 76：1353-1359, 1995. Davidovich M, Dishon A, Ilouze M & Kotler M. Susceptibility of cyprinid cultured cells to cyprinid herpesvirus 3. Arch Virol 152：1541-1546, 2007. Dishon A, Davidovich M, Ilouze M & Kotler M. Persistence of cyprinid herpesvirus 3 in infected cultured carp cells. J Virol 81：4828-4836, 2007. Dishon A, Perelberg A, Bishara-Shieban J, Ilouze M, Davidovich M, Werker S & Kotler M. Detection of carp interstitial nephritis and gill necrosis virus in fish droppings. Appl Environ Microbiol 71: 7285-7291, 2005. El-Matbouli M, Rucker U & Soliman H. Detection of Cyprinid herpesvirus-3 (CyHV-3) DNA in infected fish tissues by nested polymerase chain reaction. Dis Aquat Organ 78：23-28, 2007. El-Matbouli M, Saleh M & Soliman H. Detection of cyprinid herpesvirus type 3 in goldfish cohabiting with CyHV-3-infected koi carp (Cyprinus carpio koi). Vet Rec 161：792-793, 2007. Enzmann PJ. Rapid identification of VHSV-virus from trout by inmunofluorescense. In: Karger, S. Ed.., International Symposium on Fish Biologics: Serodiagnostics and Vaccines, Vol. 49. Developments in Biological Standardization, Leetown, WV, USA, 57–62, 1981 Estepa A, Frias D & Coll JM. Susceptibility of trout kidney macrophages to viral haemorrhagic septicemia virus. Viral Immunol 4：283–292, 1992. Faisal M & Ahne W . A cell line (CLC) of adherent peripheral blood mononuclear leucocytes of normal common carp Cyprinus carpio. Dev Comp Immunol 14：255–260, 1990. Falk K, Press CM, Landsverk T & Dannevig BH. Spleen and kidney of Atlantic salmon (Salmo salar L.) show histochemical changes early in the course of experimentally induced infectious salmon anaemia (ISA). Vet Immunol Immunopathol 49：115–126, 1995. Flano E, Husain SM, Sample JT, Woodland DL & Blackman MA. Latent Murine g-Herpesvirus Infection Is Established in Activated B Cells, Dendritic Cells, and Macrophages. J Immunol 165：1074-1081, 2000. Flano E, Kim IJ, Moore J, Woodland DL & Blackman MA. Differential g-Herpesvirus Distribution in Distinct Anatomical Locations and Cell Subsets During Persistent Infection in Mice. J Immunol 170：3828-3834, 2003. Gilad O, Yun S, Adkison MA, Way K, Willits NH, Bercovier H & Hedrick RP. Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi. J Gen Virol 84：2661-2667, 2003. Gilad O, Yun S, Andree KB, Adkison MA, Zlotkin A, Bercovier H, Eldar A & Hedrick RP. Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi. Dis Aquat Organ 48：101-108, 2002. Gilad O, Yun S, Zagmutt-Vergara FJ, Leutenegger CM, Bercovier H & Hedrick RP. Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aquat Organ 60：179-187, 2004. Gray WL, Mullis L, LaPatra SE, Groff JM & Goodwin A. Detection of koi herpesvirus DNA in tissues of infected fish. J Fish Dis 25：171-178, 2002. Gray WL, Williams RJ, Jordan RL & Griffin B. Detection of channel catfish virus DNA in latently infected catfish. J Gen Virol 80：1817–1822, 1999. Grimmett SG, WargJV, Getchell RG, Johnson DJ & BowserPR. An Unusual Koi Herpesvirus Associated with a Mortality Event of Common Carp Cyprinus carpio in New York State, USA. J Wildl Dis 42：658–662, 2006. Gunimaladevi I, Kono T, Venugopal MN & Sakai M. Detection of koi herpesvirus in common carp, Cyprinus carpio L., by loop-mediated isothermal amplification. J Fish Dis. 27：583-589, 2004. Hedrick RP, Gilad O, Yun S, Spangenberg JV, Marty GD, Nordhausen RW, Kebus MJ, Bercovier H & Eldar A. A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. J Aquat Anim Health 12：44-57, 2000. Hedrick RP, Waltzek TB & McDowell TS. Susceptibility of koi carp, common carp, goldfish, and goldfish × common carp hybrids to cyprinid herpesvirus-2 and herpesvirus-3. J Aquat Anim Health 18：26-34, 2006. Hutoran M, Ronen A, Perelberg A, Ilouze M, Dishon A, Bejerano I, Chen N & Kotler M. Description of an as yet unclassified DNA virus from diseased Cyprinus carpio species. J Virol. 79：1983-1991, 2005. Iglesias GJ, Pijoan C & Molitor T. Interactions of Pseudorabies virus with swine alveolar macrophages I: virus replication. Arch Virol 104：107-115, 1989. Iglesias GJ, Trujano M, Lokensgard J & Molitor T. Study of the potential involvement of pseudorabies virus in swine respiratory disease. Can J Vet Res 56：74-77, 1992. Ilouze M, Dishon A, Kahan T & Kotler M. Cyprinid herpes virus-3 (CyHV-3) bears genes of genetically distant large DNA viruses. FEBS Lett 580：4473-4478, 2006. Johansen LH & Sommer AI. Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. J Fish Dis 18：147-156, 1995. Lovy J, Speare DJ, Stryhn H & Wright GM. Effects of dexamethasone on host innate and adaptive immune responses and parasite development in rainbow trout Oncorhynchus mykiss infected with Loma salmonae. Fish Shellfish Immunol 24：649-658, 2008. McGeoch DJ, Rixon FJ & Davison AJ. Topics in herpesvirus genomics and evolution. Virus Res 117：90-104, 2006. Michelson S. Interaction of human cytomegalovirus with monocytes / macrophages: a love-hate relationship. Pathol Biol 45：146-158, 1997. Mims CA & Gould AJ. The Role of Macrophages in Mice Infected with Murine Cytomegalovirus. J Gen Virol 41：143-153, 1978. Morahan PS, Connor JR & Leary KR. Viruses and the versatile macrophage. Br Med Bull 41：15–21,1985. Morahan PS, Mama S, Anaraki F & Leary K. Molecular localization of abortive infection of resident peritoneal macrophages by herpes simplex virus type 1. J Virol 63：2300-2307, 1989. Perelberg A, Smirnov M, Hutoran M, Diamant A, Bejerano Y & Kotler M. Epidemiological description of a new viral disease afflicting cultured Cyprinus carpio in Israel. Isr J Aquac Bamidgeh 55：5-12, 2003. Pikarsky E, Ronen A, Abramowitz J, Levavi-Sivan B, Hutoran M, Shapira Y, Steinitz M, Perelberg A, Soffer D & Kotler M. Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus. J Virol 78：9544-9551, 2004. Plaeger-Marshall S, Ank BJ, Altenburger KM, Pizer LI, Johnston RB Jr & Stiehm ER. Replication of herpes simplex virus in blood monocytes and placental macrophages from human neonates. Pediatr Res. 26：135-139, 1989. Revoltella RP, Vigneti E, Fruscalzo A, Park M, Ragona G, Rocchi G & Calef E. Epstein-Barr Virus DNA Sequences in Precursor Monocyte-Macrophage Cell Lines Established from the Bone Marrow of Children with Maturation Defects of Haematopoiesis. J Gen Virol 70：1203-1215 1989. Rolland JB, Bouchard DA & Winton JR. The ASK cell line: an improved diagnostic tool for isolation, propagation and titration of the infectious salmon anaemia virus (ISAV). Proceedings of the ISA Symposium, IVth International Symposium on Aquatic Animal Health (New Orleans, Louisiana, USA, 3–4 September 2002). Ronen A, Perelberg A, Abramowitz J, Hutoran M, Tinman S, Bejerano I, Steinitz M & Kotler M. Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio. Vaccine 21：4677-4684, 2003. Rosenkranz D, Klupp BG, Teifke JP, Granzow H, Fichtner D, Mettenleiter TC & Fuchs W. Identification of envelope protein pORF81 of koi herpesvirus. J Gen Virol 89：896-900, 2008. Sadler J, Marecaux E & Goodwin AE. Detection of koi herpes virus (CyHV-3) in goldfish, Carassius auratus (L.), exposed to infected koi. J Fish Dis 31：71-72, 2008. Sano M, Ito T, Kurita J, Yanai T, Watanabe N, Miwa S & Iida T. First detection of koi herpesvirus in cultured common carp Cyprinus carpio in Japan. Fish Pathol 39：165-167, 2004. Shanley JD & Pesanti EL. Replication of murine cytomegalovirus in lung macrophages: effect of phagocytosis of bacteri a. Infect Immun 29：1152-1159, 1980 Soliman H & El-Matbouli M. An inexpensive and rapid diagnostic method of Koi Herpesvirus (KHV) infection by loop-mediated isothermal amplification. Virol J Dnlm 2：83-90, 2005. St-Hilaire S, Beevers N, Way K, Le Deuff RM, Martin P & Joiner C. Reactivation of koi herprsvirus infections in common carp Cyprinus carpio. Dis Aquat Organ 67： 15-23, 2005. Steinhagen D, Hespe K, Ellmer B & Körting W. Goussia carpelli (Protozoa: Coccidia) infection in stressed and immunosuppressed common carp Cyprinus carpio. Dis Aquat Organ 34：199-204, 1998. Tafalla C, FiguerasA & NovoaB. In vitro interaction of viral haemorrhagic septicaemia virus and leukocytes from trout Oncorhynchus mykiss and turbot Scophthalmus maximus. Vet Mmunol Immunopathol 62：59-66, 1998. Tu C, Weng MC, Shiau JR & Lin SY. Detection of koi herpesvirus in koi Cyprinus carpio in Taiwan. Fish Pathol 39：109–110, 2004. Waltzek TB, Kelley GO, Stone DM, Way K, Hanson L, Fukuda H, Hirono I, Aoki T, Davison AJ & Hedrick RP. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae. J Gen Virol 86：1659-1667, 2005. Weck KE, Kim SS, Virgin Iv HW & Speck SH. Macrophages Are the Major Reservoir of Latent Murine Gammaherpesvirus 68 in Peritoneal Cells. J Virol 73：3273-3283, 1999. Wise JA, Bowser PR & Boyle JA. Detection of channel catfish virus in asymptomatic adult channel catfish, Ictalurus punctatis (Rafinesque). J Fish Dis 8：485-493, 1985. Wu L, Morahan PS & Leary K. Mechanisms of intrinsic macrophage–virus interactions in vitro. Microbiol Pathol 9：293-301,1990. Yamamoto T. Infectious pancreatic necrosis (IPN) virus carriers and antibody production in a population of rainbow trout (Salmo gairdneri). Can J Microbiol 21：343-347, 1975. Yu KK, Macdonald RD & Moore AR. Replication of infectious panereatie neerosis virus in trout leucocytes and detection of the carrier state. J Fish Dis 5：401-410, 1982. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/40368 | - |
| dc.description.abstract | 錦鯉疱疹病毒koi herpesvirus (KHV)，為鯉魚疱疹病毒第三型 (Cyprinid herpes virus-3，CyHV-3) 對錦鯉及鯉魚造成嚴重的感染。KHV是在1998年由Hedrick等從以色列及美國的發病鯉魚與錦鯉分離出來。以後各國陸續有疫情的爆發，2002涂等證實台灣有KHV的發生。
本實驗室以原位雜交 (in situ hybridization) 方法定位病毒的胸腺嘧啶激酶 (thymidine kinase) 基因核酸發現發病魚組織中的病毒核酸訊號不強，但多數訊號是存在吞噬細胞中。故本研究的目的是要了解KHV會在錦鯉的吞噬細胞中複製或只是被錦鯉的吞噬細胞所吞噬後，而被機械性的攜帶或被殺死分解掉。所以本實驗分別自帶病毒錦鯉魚和健康錦鯉魚分離出吞噬細胞，攻毒KHV後在23 ℃恆溫下培養，再利用real time PCR來偵測吞噬細胞內病毒量是否增加。結果顯示，KHV的DNA在帶源魚及健康魚體外攻毒KHV的吞噬細胞中確實有增加，故可知KHV可在吞噬細胞中增殖。 | zh_TW |
| dc.description.abstract | Koi herpes virus (KHV), also called Cyprinid herpes virus-3 (CyHV-3), can cause serious disease in koi (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio). It was confirmed for the first time in the carps in Israel and the United States during the outbreak in 1998. The disease has soon spread all over the world, including Taiwan in 2002.
Our previous data showed KHV DNA located by thymidine kinase gene were observed in macrophages of diseased koi using in situ hybridization morphologically. The aim of this study is to verify whether the KHV detected in macrophage is able to replicate or just carried mechanically within macrophage. Phagocytes isolated from KHV infected koi and KHV had been inoculated to phagocytes which were isolated from normal koi were cultured in 23 ℃. Then the KHV copy numbers in the phagocytes were quantified periodically by real time PCR. Since KHV genome copies of tested phagocytes was increased, it indicates that KHV propagated in phagocytes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-14T16:45:52Z (GMT). No. of bitstreams: 1 ntu-97-R95629023-1.pdf: 37877752 bytes, checksum: 470d4daa13161440dcb1b994aaa17eea (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 目錄......................................................I
圖次......................................................V 表次....................................................VII 中文摘要...............................................VIII 英文摘要.................................................IX 第一章 緒論...............................................1 第二章 文獻回顧...........................................3 第一節 錦鯉疱疹病毒之簡介.............................3 1-1 病毒型態與命名分類..............................3 1-2 病原特性........................................4 1-3 臨床症狀，剖檢病變與病理變化....................5 1-4 發病因子-水溫...................................6 1-5 魚隻感受性......................................7 1-6 傳播途徑........................................8 1-7 病毒分離........................................8 1-8 診斷方法........................................9 第二節 其他疱疹病毒之簡介與吞噬細胞之關係.............9 1-1 Alphaherpesvirinae.............................10 1-2 Betaherpesvirinae..............................11 1-3 Gammaherpesvirinae.............................11 1-4 Alloherpesviridae..............................12 第三節 其他魚類病原與吞噬細胞之關係..................13 3-1 傳染性胰臟壞死病毒.............................13 3-2 病毒性出血性敗血症病毒.........................14 3-3 傳染性鮭魚貧血症病毒...........................15 第三章 材料與方法........................................16 第一節 實驗設計......................................16 第二節 實驗材料......................................17 2-1 PCR檢測KHV.....................................17 2-1.1 病魚組織及細胞去氧核糖核酸之萃取...........17 2-1.2 聚合酶鏈鎖反應 (Polymerase chain reaction, PCR)...........17 2-1.3 瓊脂凝膠電泳 (Agarose gel electrophoresis).......17 2-2 即時定量系統 (Real time PCR) 定量標準品 (standard) 的準備.....18 2-2.1聚合酶鏈鎖反應 (Polymerase chain reaction, PCR).......18 2-2.2 接合作用...................................18 2-2.3 抽取質體...................................18 2-2.4 限制酶切割.................................18 2-3 即時定量系統 (Real time PCR) 檢測KHV病毒量.....19 2-4 原位雜交 (In situ hybridization; ISH)..........19 2-4.1 探針 (probe) 之合成........................19 2-4.2點墨雜交法 (dot-blot hybridization).........19 2-4.3原位雜交 (In situ hybridization; ISH).......20 2-5 不同感染途徑病毒出現量之比較...................21 2-6 KHV感染錦鯉吞噬細胞後病毒量的分析..............21 第三節 實驗方法......................................22 3-1 PCR檢測KHV.....................................22 3-1.1 病魚組織去氧核醣核酸 (DNA) 之萃取..........22 3-1.2 細胞去氧核醣核酸 (DNA) 之萃取..............23 3-1.3 KHV聚合酶鏈鎖反應 (Polymerase chain reaction, PCR) ..........23 3-1.4 瓊脂凝膠電泳 (Agarose gel electrophoresis).24 3-1.5 核酸定序及序列比對.........................25 3-2即時定量系統 (Real time PCR) 定量標準品 (standard) 的準備.......25 3-2.1 聚合酶鏈鎖反應 (Polymerase chain reaction, PCR)..............25 3-2.2 接合作用...................................26 3-2.3 抽取質體...................................26 3-2.4 限制酶切割.................................27 3-2.5 建立定量標準品.............................27 3-3 即時定量系統 (Real time PCR) 檢測KHV病毒量.....28 3-4 原位雜交 (In situ hybridization; ISH)..........28 3-4.1 KHV探針之合成..............................28 3-4.2點墨雜交法 (dot-blot hybridization).........29 3-4.3 原位雜交 (In situ hybridization; ISH)......29 3-5不同感染途徑病毒出現量之比較....................30 3-5.1 腹腔注射 (intraperitoneal injection，i.p)..30 3-5.2免疫抑制....................................31 3-5.3同居感染 (cohabitation).....................31 3-5.4 體表有傷口錦鯉同居感染.....................31 3-6 KHV感染錦鯉吞噬細胞後病毒量的分析..............31 3-6.1 頭腎及脾臟巨噬細胞的分離...................31 3-6.2 血液單核球的分離...........................32 3-6.3 帶原錦鯉來源吞噬細胞內病毒量分析...........33 3-6.4 KHV感染健康錦鯉之吞噬細胞後病毒量分析......33 第四章 實驗結果..........................................35 1 PCR檢測KHV.........................................35 2 KHV定量標準品 (standard) 的準備....................35 3 點墨雜交法 (dot-blot hybridization)................36 4 原位雜交 (In situ hybridization; ISH)..............36 5不同感染途徑病毒出現量之比較........................36 5.1腹腔注射 (intraperitoneal injection，i.p.)......36 5.2免疫抑制........................................37 5.3同居感染........................................37 5.4體表有傷口錦鯉同居感染..........................38 6 KHV感染錦鯉吞噬細胞後病毒量的分析..................38 6.1頭腎與脾臟巨噬細胞及血液單核球的分離............38 6.2帶病毒錦鯉來源吞噬細胞的病毒量分析..............38 6.3 KHV感染健康錦鯉之吞噬細胞後病毒量分析..........40 第五章 討論..............................................52 第六章 參考文獻..........................................59 圖次 圖一、KHV DNA的檢測......................................41 圖二、KHV 即時定量系統 (Real time PCR) stardand curve之建立..............41 圖三、KHV探針之合成......................................42 圖四、點墨雜交法.........................................42 圖五、利用原位雜交來定位鰓和腎臟組織中KHV的核酸..........43 圖六、利用原位雜交來定位腸道和脾臟組織中KHV的核酸........44 圖七、不同感染途徑病毒出現量之比較。 A健康錦鯉i.p.攻毒KHV乳劑後不同時間臟器所含病毒量...45 B加免疫抑制劑攻毒後不同時間臟器所含病毒量..........45 C健康錦鯉與KHV陽性錦鯉同居後不同時間臟器所含病毒量.45 D體表有傷口錦鯉與KHV陽性錦鯉同居後不同時間臟器所含病毒量..45 E不同感染途徑腎臟病毒含量之比較....................45 F不同感染途徑脾臟病毒含量之比較....................45 G不同感染途徑肝臟病毒含量之比較....................45 圖八、A為由頭腎及脾臟所分離出來白血球....................46 B去除懸浮細胞後主要以巨噬細胞為主..................46 圖九、A為由血液所分離出得白血球..........................46 B為去除懸浮細胞後的單核球..........................46 圖十、比較添加LPS後細胞存活率 A比較添加LPS後細胞存活率及細胞內病毒含量............47 B比較添加LPS後細胞存活率及細胞外病毒含量............47 C比較添加LPS後細胞存活率及細胞內及細胞外病毒總量....47 圖十一、添加ECP、LPS後細胞的存活率及病毒變化量 A比較添加ECP或LPS後細胞的存活率及細胞內病毒含量.....48 B比較添加ECP或LPS後細胞的存活率及細胞外病毒含量.....48 C比較添加ECP或LPS後細胞的存活率及細胞內和細胞外病毒總量......48 圖十二、健康吞噬細胞體外攻毒後病毒量分析。 A健康吞噬細胞體外攻毒後細胞的存活率及細胞內病毒含量.49 B健康吞噬細胞體外攻毒後細胞的存活率及細胞外病毒含量.49 C健康吞噬細胞體外攻毒後細胞的存活率及細胞內及細胞外病毒總量....49 表次 表一、健康錦鯉攻毒KHV乳劑後不同時間臟器所含病毒量........50 表二、添加免疫抑制劑攻毒後不同時間臟器所含病毒量.........50 表三、健康錦鯉與KHV陽性錦鯉同居後不同時間臟器所含病毒量..51 表四、體表有傷口錦鯉與KHV陽性錦鯉同居後不同時間臟器所含病毒量.......51 | |
| dc.language.iso | zh-TW | |
| dc.subject | 錦鯉 | zh_TW |
| dc.subject | 錦鯉皰疹病毒 | zh_TW |
| dc.subject | 吞噬細胞 | zh_TW |
| dc.subject | koi | en |
| dc.subject | koi herpesvirus | en |
| dc.subject | phagocytes | en |
| dc.title | 錦鯉疱疹病毒在錦鯉吞噬細胞中複製之可能性之探討 | zh_TW |
| dc.title | Study on the Possible Fate of Koi Herpesvirus in the Koi Phagocytes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 施秀惠,張本恆,涂堅 | |
| dc.subject.keyword | 錦鯉,錦鯉皰疹病毒,吞噬細胞, | zh_TW |
| dc.subject.keyword | koi,koi herpesvirus,phagocytes, | en |
| dc.relation.page | 67 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-31 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-97-1.pdf 未授權公開取用 | 36.99 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。