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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝旭亮 | |
| dc.contributor.author | Chih-Hao Chen | en |
| dc.contributor.author | 陳智豪 | zh_TW |
| dc.date.accessioned | 2021-06-13T17:30:50Z | - |
| dc.date.available | 2014-07-25 | |
| dc.date.copyright | 2011-07-25 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-11 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39526 | - |
| dc.description.abstract | 在模式植物阿拉伯芥的研究中，已知光敏素A (phytochrome A) 是負責接收遠紅光的光接受體，並且藉由一系列的分子將遠紅光的訊息傳遞到細胞內。FAR-RED INSENSITIVE 219 (FIN219)/JAR1 是作用在光敏素A訊息下游的一正向因子，並且其經由轉錄、轉譯後產生的蛋白質為一酵素，可以催化茉莉酸 (Jasmonic acid) 接上異亮氨酸 (Isoleucine)。而本篇研究發現在fin219-2突變株中，光敏素A訊息中的負向調控因子SUPPRESSOR OF PHYA-105 1 (SPA1) 的轉錄表現量大量上升；為了瞭解FIN219和SPA1之間的基因調控關係，我們從雙突變株miSPA1/fin219-2，以及SPA1大量表現在fin219-2突變株(SPA1ox/fin219-2) 和FIN219大量表現在spa1突變株 (FIN219ox/spa1)的阿拉伯芥轉殖株的性狀觀察得知在遠紅光訊息中，SPA1是位在FIN219的下游。藉由分析spa1突變株的性狀，顯示下胚軸對於茉莉酸的反應需要SPA1，此外從基因表現檢測的結果發現茉莉酸所誘導表現的基因VSP1和MYC2都受到SPA1的正向調控，兩者顯示SPA1確實正向參與在茉莉酸的訊息傳遞之中。因此在遠紅光下，FIN219對於SPA1的抑制作用提供茉莉酸訊息傳遞中一個負回饋機制，來減緩植物對於茉莉酸的反應強度；此外分析轉殖株對於茉莉酸的反應，發現跟SPA1相比，FIN219在茉莉酸訊息中為主要的調控者。綜合研究的結果，顯示FIN219和SPA1之間的調控關係，反應出遠紅光訊息以及茉莉酸訊息之間的交互作用，以及對於彼此訊息傳遞的影響。 | zh_TW |
| dc.description.abstract | In Arabidopsis, phytochrome A is one of the photoreceptors and responsible for far-red light signal transduction. FAR-RED INSENSITIVE 219 (FIN219)/JAR1 is a positive component in the downstream of phytochrome A signaling and encodes a jasmonic acid (JA)-amido synthatase, catalyzing the synthesis of JA-isoleucine (JA-Ile). In this study, it is found that the transcript of SUPPRESSOR OF PHYA-105 1 (SPA1), a repressor of phytochrome A-mediated responses, is greatly enhanced in the fin219-2 mutant under continuous far-red light (FRc). To determine functional regulation between FIN219 and SPA1, we generated the mimic double mutant (miSPA1/fin219-2), SPA1 overexpression in fin219-2 (SPA1ox/fin219-2), and FIN219 overexpression in spa1 (FIN219ox/spa1) transgenic plants, and the results showed that SPA1 acts downstream of FIN219 in FRc. Phenotypic analysis of the spa1 mutant suggests that SPA1 is required for hypocotyl response to MeJA; besides, Real-time PCR results indicated that the induction of two JA-responsive genes, VSP1 and MYC2, is reduced in spa1 mutant. These data indicate that SPA1 is a positive regulator in JA signaling under FRc condition. Thus, FIN219 may serve as a negative feedback through inhibiting SPA1 to control the magnitude and duration of JA responses in far-red light. By examining JA responses of these transgenic plants, the results reveal that FIN219 plays a major role in JA signaling compared with SPA1. Taken together, these data indicate that FIN219 and SPA1 show genetic interactions and regulatory relation to provide a critical mechanism in the integration of far-red light and JA signaling. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T17:30:50Z (GMT). No. of bitstreams: 1 ntu-100-R98b42013-1.pdf: 2019562 bytes, checksum: 41fc02ab6c661594a86d8336832953f6 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | Table of Contents
INTRODUCTION 1 Light and Photoreceptors 1 Two Key Signaling Components in Light: HY5 and COP1 2 Negative Regulator in Far-Red Light: SPA1 3 Positive Regulator in Far-Red Light: FIN219 4 Jasmonate Signaling 4 The Crosstalk between Light and JA Signaling 5 RESULTS 7 SPA1 is required for JA responses during germination and acts positively in MeJA response to modulate hypocotyl elongation in FRc. 7 FIN219 negatively regulates the transcriptional expression of SPA1 in various light conditions except for Rc. 8 Artificial miSPA1 represses SPA1 expression in fin219-2. Regardless of FIN219, MeJA treatment inhibits SPA1 expression in FRc. 10 FIN219 acts upstream of SPA1 in FR signaling and plays a major role in JA responses compared to SPA1. 10 The regulation of SPA1 transcriptional expression does not involve PIFs and HY5. 11 SPA1 is necessary for the induction of some JA-responsive genes in FRc. 13 SPA1 overexpression rescues the phenotypes of spa1. 14 DISSCUSION 15 MATERIAL AND METHODS 20 Plant Material 20 Plant Growth Conditions 20 Plasmid Construction 21 Total Genomic DNA Isolation 23 Total Protein Isolation and Western Blotting 23 Total RNA Isolation, Northern blotting, RT-PCR, and Quantitative Real-Time PCR 24 Measurement of Hypocotyls and Roots 24 FIGURES 25 REFERENCES 39 APPENDIX I 43 APPENDIX II 45 APPENDIX III 46 APPENDIX IV 47 APPENDIX V 48 | |
| dc.language.iso | en | |
| dc.subject | FIN219 | zh_TW |
| dc.subject | 茉莉酸 | zh_TW |
| dc.subject | SPA1 | zh_TW |
| dc.subject | 遠紅光 | zh_TW |
| dc.subject | jasmonic acid | en |
| dc.subject | SPA1 | en |
| dc.subject | FIN219 | en |
| dc.subject | far-red light | en |
| dc.title | FIN219和SPA1在遠紅光及茉莉酸訊息傳遞中的功能性研究 | zh_TW |
| dc.title | Functional Studies of FIN219 and SPA1 in the Integration of Far-Red Light and Jasmonic Acid Signaling in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王淑美,鄭石通,張孟基,吳素幸 | |
| dc.subject.keyword | 遠紅光,茉莉酸,FIN219,SPA1, | zh_TW |
| dc.subject.keyword | far-red light,jasmonic acid,FIN219,SPA1, | en |
| dc.relation.page | 48 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-07-11 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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