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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 宋孔彬(Kung-Bin Sung) | |
| dc.contributor.author | Yi-Chang Lai | en |
| dc.contributor.author | 賴怡璋 | zh_TW |
| dc.date.accessioned | 2021-06-13T17:30:40Z | - |
| dc.date.available | 2013-08-23 | |
| dc.date.copyright | 2011-08-23 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-19 | |
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Kim, et al., 'Low-coherence enhanced backscattering: review of principles and applications for colon cancer screening,' Journal of Biomedical Optics, vol. 11, p. 041125, 2006. [9] F. E. Robles and A. Wax, 'Measuring morphological features using light-scattering spectroscopy and Fourier-domain low-coherence interferometry,' Opt. Lett., vol. 35, pp. 360-362, 2010. [10] R. N. Graf, et al., 'Parallel frequency-domain optical coherence tomography scatter-mode imaging of the hamster cheek pouch using a thermal light source,' Opt. Lett., vol. 33, pp. 1285-1287, 2008. [11] F. E. Robles and A. Wax, 'Measuring structural features using a dual window method for light scattering spectroscopy and Fourier-domain low coherence interferometry,' pp. 757310-757310-4, 2010. [12] R. N. Graf, et al., 'Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations,' Journal of Biomedical Optics, vol. 14, p. 064030, 2009. [13] F. Robles, et al., 'Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolution,' Opt. Express, vol. 17, pp. 6799-6812, 2009. [14] 巢國山, '利用一個脈衝波的有限差分時域法來模擬寬波長範圍的細胞散射光.' [15] E. Alarousu, Low coherence interferometry and optical coherence tomography in paper measurements: Oulu university press, 2006. [16] M. Brezinski, Optical Coherence Tomography Principles and Applications: Elsevier, 2006. [17] H. C. v. d. Hulst, Light Scattering by Small Particles: Dover, 1981. [18] J. R. Mourant, et al., 'Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms,' Appl. Opt., vol. 36, pp. 949-957, 1997. [19] J. R. Mourant, et al., 'Mechanisms of Light Scattering from Biological Cells Relevant to Noninvasive Optical-Tissue Diagnostics,' Appl. Opt., vol. 37, pp. 3586-3593, 1998. [20] V. Backman, et al., 'Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ,' Selected Topics in Quantum Electronics, IEEE Journal of, vol. 5, pp. 1019-1026, 1999. [21] A. F. Fercher and et al., 'Optical coherence tomography - principles and applications,' Reports on Progress in Physics, vol. 66, p. 239, 2003. [22] U. Morgner, et al., 'Spectroscopic optical coherence tomography,' Opt. Lett., vol. 25, pp. 111-113, 2000. [23] A. L. Oldenburg, et al., 'Spectroscopic Optical Coherence Tomography and Microscopy,' Selected Topics in Quantum Electronics, IEEE Journal of, vol. 13, pp. 1629-1640, 2007. [24] 維基百科-時頻分析, 'http://zh.wikipedia.org/wiki/%E6%99%82%E9%A0%BB%E5%88%86%E6%9E%90.' [25] R. N. Graf and A. Wax, 'Temporal coherence and time-frequency distributions in spectroscopic optical coherence tomography,' J. Opt. Soc. Am. A, vol. 24, pp. 2186-2195, 2007. [26] B. W. Pogue and M. S. Patterson, 'Review of tissue simulating phantoms for optical spectroscopy, imaging and dosimetry,' Journal of Biomedical Optics, vol. 11, p. 041102, 2006. [27] M. Xiaoyan and et al., 'Determination of complex refractive index of polystyrene microspheres from 370 to 1610 nm,' Physics in Medicine and Biology, vol. 48, p. 4165, 2003. [28] J. Franklin and Z. Y. Wang, 'Refractive Index Matching: A General Method for Enhancing the Optical Clarity of a Hydrogel Matrix,' American Chemical Society, vol. 14, 2002. [29] J. R. Tse and A. J. Engler, Preparation of Hydrogel Substrates with Tunable Mechanical Properties: John Wiley & Sons, Inc., 2010. [30] X. J. Liang, et al., 'Determining refractive index of single living cell using an integrated microchip,' Sensors and Actuators A: Physical 133(2), pp. 349-354, 2007. [31] G.-S. Chao and K.-B. Sung, 'Investigating the spectral characteristics of backscattering from heterogeneous spheroidal nuclei using broadband finite-difference time-domain simulations,' San Francisco, California, USA, 2010, pp. 75730E-6. [32] R. Y. Tsien, 'The Green Fluorescent Protein,' Annu. Rev. Biochem, 1998. [33] W. Drexler and J. G. Fujimoto, Optical Coherence Tomography Technology and Applications: Springer, 2008. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39520 | - |
| dc.description.abstract | 目前許多研究指出光散射光譜對於結構與折射率的變化非常敏感；藉由分析初期癌化細胞的逆散射光譜，可以得到細胞在病變過程中，細胞核大小變化的資訊以及折射率的改變。因此光散射光譜在生醫工程的運用上，可提供細胞初期癌化時，結構變化的資訊。因此是一種有潛力實現非侵入式上皮細胞初期癌化檢測之技術。
本研究之目的希望能建立一套實驗系統及方法，來準確測量並分析上皮細胞之逆散射光譜，因為許多癌症均發源於上皮細胞。本論文主要提出了一光學量測架構，來測量上皮細胞細胞核的逆散射光譜。除了設計實驗來驗證系統的可行性外，也測量了人類胚胎上顎間質細胞及癌化上皮鱗狀細胞的逆散射光譜，並與Mie theory (米氏定律)之理論值作比對，以求得細胞核的大小，進而判斷細胞是否產生癌化，並建立光譜分析的資料庫。 同時本架構也加上了頻率域光學同調斷層掃描(FD-OCT)技術，更進一步蒐集逆散射光，並利用光譜式光學同調斷層掃描術(SOCT)的時頻分析方式，來提高空間以及頻譜解析度，目前已經能藉由此架構分析蓋玻片的厚度，未來希望能藉由FD-OCT來準確定量出聚苯乙烯球的大小，接著更進一步來計算細胞核的厚度。 | zh_TW |
| dc.description.abstract | Recently, many researches indicate that light scattering spectroscopy (LSS) is greatly influenced by the change of structure and refractive index. By analyzing the backscattering light spectroscopy of early stage cancer cells, the changes of their nuclear size and refractive index during the development of precancerous lesions can be revealed. Therefore, LSS is a promising technique for noninvasively detecting precancerous lesions in vivo.
This thesis builds up an optical system and research methods for measuring and analyzing the backscattering light of individual epithelial cells. Our optical system contains two part, one part can directly measure the LSS form living cell. The other part combines the LSS technique with low coherence interferometry (LCI) which is called Fourier domain optical coherence tomography (FD-OCT) to detect the depth resolved backscattering signal form an object. The spectroscopic optical coherence tomography (SOCT) method is adapted to find out more scattering information. In this thesis, I use our optical system to examine the relationship between the backscattered light of cell nucleus and enlargement of cancer cell nucleus. First, I validate our optical system with polystyrene beads and then measure the backscattering spectroscopy of living cells including human embryonic palatal mesenchymal (HEPM) cells and oral squamous carcinoma cells (CA9-22). I find out the nuclear size by fitting the result with Mie theory. Also, I characterize the axial resolution of the FD-OCT part of the system and use the system to measure the thickness of a coverslip. In the future, I will use the FD-OCT and SOCT to find out the size of polystyrene beads and then investigate the structural information of cell nucleus. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T17:30:40Z (GMT). No. of bitstreams: 1 ntu-100-R97945051-1.pdf: 4283474 bytes, checksum: 1e5368ff3a2dc5c27e11ce8d887c8a00 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 誌謝 I
中文摘要 II ABSTRACT III 目錄 V 圖目錄 VIII 表目錄 XIII 第一章 導論 - 1 - 1-1 研究背景 - 1 - 1-2 研究動機及目標 - 2 - 1-3 論文架構 - 3 - 第二章 理論介紹 - 5 - 2-1 光散射光譜簡介 - 5 - 2-1.1 光的散射 - 5 - 2-1.2 米氏定律(Mie theory) - 7 - 2-1.3 光散射光譜在生醫上的運用 - 8 - 2-2 光學同調斷層掃瞄簡介 - 9 - 2-2.1 麥克森干涉儀 - 10 - 2-2.2 低同調干涉理論 - 13 - 2-2.3 軸向解析度(Axial resolution) - 16 - 2-2.4 時域光學同調斷層掃瞄術 (Time domain OCT) - 17 - 2-2.5 頻域光學同調斷層掃瞄術 (Spectral domain OCT) - 18 - 2-2.6 光譜式光學同調斷層掃瞄術 (Spectroscopic Optical Coherence Tomography；SOCT) - 22 - 第三章 研究架構及校正方法 - 26 - 3-1 光散射光譜架構介紹 - 26 - 3-1.1 光散射光譜架構 - 26 - 3-1.2 光路校正方法 - 31 - 3-2 頻域光學同調斷層掃描架構介紹 - 35 - 3-2.1 頻域光學同調斷層掃描架構 - 35 - 3-2.2 干涉訊號的調整及最佳化 - 36 - 第四章 實驗設計與結果討論 - 42 - 4-1 光散射光譜系統之驗證 - 42 - 4-1.1 光散射光譜標準樣本備製 - 42 - 4-1.2 散射光譜分析方法 - 44 - 4-1.3 與理論值比對 - 47 - 4-2 活上皮細胞實驗 - 51 - 4-2.1 樣本基質設計 - 51 - 4-2.2 Mie theory參數設定 - 56 - 4-2.3 HELM細胞-細胞核逆散射光譜分析 - 58 - 4-2.4 CA9-22細胞-細胞核逆散射光譜分析 - 66 - 4-3 頻域光學同調斷層掃描架構之驗證 - 74 - 4-3.1 系統軸向解析度 - 74 - 4-3.2 蓋玻片實驗 - 76 - 4-3.3 載玻片實驗 - 79 - 4-3.4 加上水媒物鏡後的解析度 - 80 - 4-3-5 Dual window方法於聚苯乙烯球干涉之分析 - 81 - 第五章 綜合討論與結論 - 85 - 5-1 聚苯乙烯球散射實驗討論 - 85 - 5-2 活細胞實驗討論 - 87 - 5-3 FD-OCT解析度探討 - 93 - 5-4 結論與展望 - 97 - 參考資料 - 98 - | |
| dc.language.iso | zh-TW | |
| dc.title | 上皮細胞之彈性逆散射光譜的量測與分析 | zh_TW |
| dc.title | Measuring and Analyzing Epithelial Cell Elastic Backscattering Spectroscopy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃升龍(Sheng-Lung Huang),曾雪峰(Snow H. Tseng),郭文娟(Wen-Chuan Kuo) | |
| dc.subject.keyword | 光散射光譜,彈性逆散射,頻率域光學同調斷層掃描術,光譜式光學同調斷層掃描術,上皮細胞, | zh_TW |
| dc.subject.keyword | Light scattering spectroscopy,Elastic backscattering light,Fourier domain optical coherence tomography,Spectroscopic optical coherence tomography,Epithelial cell., | en |
| dc.relation.page | 100 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-20 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| Appears in Collections: | 生醫電子與資訊學研究所 | |
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| ntu-100-1.pdf Restricted Access | 4.18 MB | Adobe PDF |
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