請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39302
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李敏雄 | |
dc.contributor.author | Chien Lin | en |
dc.contributor.author | 林芊 | zh_TW |
dc.date.accessioned | 2021-06-13T17:25:40Z | - |
dc.date.available | 2005-01-31 | |
dc.date.copyright | 2005-01-31 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-01-23 | |
dc.identifier.citation | 行政院衛生署。2002。健康食品管理法之健康食品安全及功效評估方法。
吳佳倩與吳連生。1984。細菌學名詞辭典。名山出版社,台北。 張士軒。1994。靈菌的色素生成及其應用。國立台灣海洋大學水產食品科學研究所博士學位論文。 洪啟章。1994。Bacillus cereus NYU-FC-4 菌株之幾丁質酶及幾丁聚醣 酶之研究。國立台灣大學農業化學研究所碩士論文。 姚惠芳。2000。由 Serratia marcescens FC-R1 生產紅色色素之研究。國立台灣大學農業化學研究所碩士論文。 鄧素真。2000。Serratia marcescens 新的調控多細胞行為現象之 quorum- sensing 系統。國立台灣大學醫事技術學研究所碩士論文。 賴慧民。2003。由 Bacillus cereus NTU-FC-11 生產豬血塊分解酵素之研究。國立台灣大學農業化學研究所碩士論文。 蔡文城。1996。微生物學。藝軒圖書出版社,台北。 蘇遠志與黃世佑。1994。微生物化學工程學。華香園出版社,台北。 藝軒圖書出版社編輯部。1990。新編道氏醫學大辭典,藝軒圖書出版社,台北。 Alberti, L. and R. M. Hashey. 1990. Differentiation of Serratia marcescens 274 into swimmer and swamer cells. J. Bacteriol., 172:4322-4328. Allen, E. G. 1967. Conditions of the colour change of prodigiosin. Nature, 216:929-931. Barlett, W. T., G. A. O'Donovan and R. D. Neff. 1970. Effect of gamma radiation on Serratia marcescens. Studies on the radiosensitivity of prodigiosin production. Radiat. Res., 43:196-203. Bunting, M. I. 1939. A description of some color variants produced by Serratia marcescens, strain 274. J. Bacteriol., 39:57-68. Bunting, M. I. and L. J. Ingraham. 1942. The distribution of color variants in ageing broth cultures of Serratia marcescens #274. J. Bacteriol., 43:585-591. Bunting, M. I., C. F. Robinow and H. Bunting. 1949. Factors affecting the elaboration of pigment and polysaccharide by Serratia marcescens. J. Bacteriol., 58:114-115. Button, G. L., M. A. Miller and J. C. Tsang. 1975. Antibiogram and lipid analysis of a pigmented strain of Serratia marcescens and its nonpigmented variants. Antimicrob. Agents Chemother., 7:219-222. Campàs, C., M. Dalmau, B. Montaner, M. Barragán, B. Bellosillo, D. Colomer, G. Pons, R. Pérez-Tomás and J. Gil. 2003. Prodigiosin induces apoptosis of B and T cells from B-cell chronic lymphocytic leukemia. Leukemia, 17:746-750. Castro, A. J. 1967. Antimalarial activity of prodigiosin. Nature (London), 213:903-904. Castro, A. J., J. F. Deck, M. T. Hugo, L. R. Williams and M. R. Zingg. 1958. Prodigiosin hydrochloride. J. Org. Chem., 23:1232-1233. Cho, L. K. N., J. A. Lowe, R. B. Maguire and J. C. Tsang. 1987. Relationship of prodigiosin condensing enzyme activity to the biosynthesis of prodigiosin and its precursors in Serratia marcescens. Experientia, 43:397-399. Clements-Jewery, S. 1976. The reversal of glucose repressed prodigiosin production in Serratia marcescens by the cyclic 3'5'-adenosine monophosphate inhibitor theophylline. Experientia, 32:421-533. Crichton, P. V. and A. S. Lazarus. 1948. The relationship between prodigiosin production and catalase activity. J. Bacteriol., 56:375-377. Cruz-Camarillo, R. and A. A. Sanchez-Zuniga. 1968. Complex protein- protein in Serratia marcescens. Nature, 218:567-568. Datta, A. R. and M. H. Kothary. 1993. Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes. Appl. Environ. Microbiol., 59: 3495-3497. De la Fuente, G., F. Perestelo, A. Rodriguez-Oerez, and M. A. Falcon. 1991. Oxidation of aromatic aldehyde by Serratia marcescens. Appl. Environ. Microbiol., 57:1275-1276. Deol, B. S., J. R. Alden and J. L. Still. 1972. The isolation and characterization of monopyrroles from Serratia marcescens. Biochem. Biophys. Res. Commun., 47:1378-1385. Díaz-Ruiz, C., B. Montaner and R. Pérez-Tomás. 2001. Prodigiosin induces cell death and morphological changes indicative of apoptosis in gastric cancer cell line HGT-1. Histol. Histopathol., 16:415-421. Ding, M. J. and R. P. Williams. 1983. Biosynthesis of prodigiosin by white strains of Serratia marcescens isolated from patients. J. Clin. Microbiol., 17:476-480. Eaves, G. N. and C. D. Jefferies. 1963. Isolation and properties of an exocellular nuclease of Serratia marcescens. J. Bacteriol., 85:273-278. Ehrlich, R. 1954. Induced filament formation and color variations in a strain of Serratia marcescens. J. Bacteriol., 68:752-753. Feng, J. S., J. W. Webb and J. C. Tsang. 1982. Enhancement by sodium dodecyl sulfate of pigment formation in Serratia marcescens. Appl. Environ. Microbiol., 43:850-853. Fuller, A. T. and J. M. Horton. 1950. Marcescin, and antibiotic substance from Serratia marcescens. J. Gen Microbiol., 4:417-433. Fürstner, A. 2003. Chemistry and biology of reseophilin and the prodigiosin alkaloids: a survey of the last 2500 years. Angew. Chem. Int. Ed., 42:3582-3603. Grimont, P. A. D. and F. Grimont. 1978. The genus Serratia. Annu. Rev. Microbiol., 32:221-248. Grimont, P. A. D. and F. Grimont. 1984. Bergey's Manual of Systematic Bacteriology (N. R. Krieg. Ed.). Willams & Wilkins, Baltimore, USA, 1:477-484. Han, S. B., H. M. Kim, Y. H. Kim, C. W.Lee, E. Jang and K. H. Son. 1998. T-cell specific immunosuppression by prodigiosin isolated from Serratia marcescens. Int. J. Immunopharmacol., 20:1–13. Hearn, W. R., J. Medina-Castro and M.K. Elson. 1968. Colour change of prodigiosin. Nature, 220:170-171. Hearn, W. R., R. E. Worthington, R. C. Burgus and R. P. Williams. 1964. Norprodigiosin: occurrence in a mutant of Serratia marcescens. Biochem. Biophys. Res. Commun., 17:517–520. Heinemann, B., A. J. Howard and H. J. Palocz. 1970. Influence of dissolved oxygen levels on production of L-asparaginase and prodigiosin by Serratia marcescens. Appl. Microbiol., 19:800-804. Hermansson, M., S. Kjelleberg and B. Norkrans. 1979. Interaction of pigmented wildtype and pigmentless mutant of Serratia marcescens with lipid surface film. FEMS Microbiol. Lett., 6:129-132. Honma, M., M. Hayashi, H. Shimada, N. Tanaka, S. Wakuri, T. Awogi, K. I. Yamamoto, N. Ushio-Kodani, Y. Nishi, M. Nakadate and T. Sofuni. 1999. Evaluation of the mouse lymphoma tk assay (microwel method) as an alternative to the in vitro chromosomal aberration test. Mutagenesis, 14:5-22. Hubbard, R. and C. Rimington. 1950. The biosynthesis of prodigiosin, the tripyrrylmethene pigment from Bacillus prodigiosus (Serratia marcescens). Biochem. J., 46:220-225. Kalesperis, G. S., K. V. Prahlad and D. L. Lynch. 1975. Toxigenic studies with the antibiotic pigments from Serratia marcescens. Can. J. Microbiol., 21:213-220. Kamata, K., S. Okamoto, S. Oka, H. Kamata, H.Y. Agisawa, and H. Hirata. 2000. Cycloprodigiosin hydrochloride suppresses tumor necrosis factor (TNF) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39302 | - |
dc.description.abstract | Prodigiosin 為 Serratia marcescens、Pseudomonas spp.、Streptomyces spp. 等微生物所產生之紅色色素。近年來陸續有研究指出 prodigiosin 具有免疫抑制及抗癌活性,因此對於將來無論是用於機能性食品或是開發成新型免疫抑制劑及抗癌藥物,都具有很大的潛力。在本研究中欲使用 S. marcescens 大量生產 prodigiosin,結果顯示,S. marcescens BCRC 11576 以1.4% 大豆粉培養基於27℃、120 rpm培養 48 小時後,每瓶培養液 (100 mL) 產量可達 48 mg,為目前已知研究中最高者。另外,培養基組成會大幅影響 prodigiosin 之產量。添加葡萄糖使葡萄糖與氮源之比例達一定程度會抑制 prodigiosin 之生成,並且使培養液之 pH 快速下降至 3 以下。將培養液緩衝化使培養過程中 pH 維持在 7 ~ 9 之間,可使 prodigiosin 之生成不受到抑制,推論 pH 下降可能為導致 prodigiosin 生成受到抑制之因。
以1.4% 大豆粉培養基生產之培養液 1L (prodigiosin 含量為 0.46 g/L),取菌體以甲醇萃取得粗萃色素 1.9 g,經矽膠管柱純化之乙酸乙酯區分為 prodigiosin,可得 0.18 g,純度達 94%。純化後之 prodigiosin由體外鼷鼠淋巴瘤 tk 分析法得知在 0.8 ~ 8 mM 之濃度並無顯著之致突變性,增加了 prodigiosin 使用上之安全性。 | zh_TW |
dc.description.abstract | Prodigiosin is the red pigment produced by microorganisms such as Serratia marcescens, Pseudomonas spp., Streptomyces spp., etc. Recently, the immunosuppressive and apoptotic activities of prodigiosin have been described. Therefore, prodigiosin seems to be a promising compound for the usages in the functional food, new immunosuppressants and anti-cancer drugs. In this study, the yield of the prodigiosin produced by S. marcescens BCRC 11576 reached 48 mg/100mL broth after incubation in 1.4% soybean flour broth at 27℃ with vigorous shaking for 48 hours. This yield was the highest of all previously reported. Besides, it was found that the prodigiosin production of this strain was affected greatly depending on the composition of medium. When this strain was incubated in the presence of glucose with the glucose-to-nitrogen source ratio reached to a certain value, no prodigiosin was produced and the pH of the broth dropped quickly to a value below 3. Buffering the broth between pH 7 and pH 9 during incubation released the inhibition resulted from addition of glucose to the broth. Consequently, the inhibitory effect of glucose on prodigiosin production may be due to a lowering of the pH of the medium.
S. marcescens BCRC 11576 was incubated in 1.4% soybean flour broth for 48 hours (the yield of the prodigiosin was 0.46 g/L). After 1 L broth was centrifuged, the cell pellets extracted with methanol and obtained 1.9 g of the crude extract pigment. The crude extract was purified by silica gel chromatography and the prodigiosin was eluted with ethyl acetate. The amount and the purity are 0.18 g and 94%, respectively. The purified prodigiosin showed no significant mutagenesis analyzed by the mouse lymphoma tk assay with the prodigiosin concentration between 0.8 and 8 mM. This result increased the safety for the use of prodigiosin. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T17:25:40Z (GMT). No. of bitstreams: 1 ntu-94-R91623502-1.pdf: 1447358 bytes, checksum: ee0914ea4e1468a5ec1f42afbb3c5501 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目次………………………………………………………………………..….Ⅰ
圖次…………………………………………………………………………...Ⅲ 表次………………………………………………………………………….Ⅵ中文摘要……………………………………………………………………. Ⅶ 英文摘要……………………………………………………………………. Ⅷ 壹、前言…………………………………………………………………….......1 貳、文獻整理……………………………………………………………….......2 一、Prodigiosin 之由來、生合成與功用………………………….............2 二、Serratia marcescens 之一般特性與應用………………………….....9 三、Prodigiosin 之培養條件與生產研究………………………….........20 參、材料與方法…………………………………………………………….....29 一、實驗架構………………………………………………………….....29 二、材料………………………………………………………….............29 三、儀器………………………………………………………….............33 四、實驗方法………………………………………………………….....34 肆、結果與討論………………………………………………………….........51 一、選擇高 prodigiosin 產量之菌株……………………………….......51 二、Prodigiosin 生產條件之探討……………………………….............55 三、葡萄糖抑制 prodigiosin 生成之探討……………………………...80 四、Prodigiosin 之純化……………………………….............................89 五、HPLC 定量方法之建立………………………….............................97 六、純化後 prodigiosin 之安全性試驗評估……………......................98 七、粗酵素液之酵素活性……………...................................................100 伍、結論…………….................................…………........…………..............103 陸、參考文獻…….................................…………........…………..................104 柒、附錄…….................................…………........…………..........……........114 | |
dc.language.iso | zh-TW | |
dc.title | 由 Serratia marcescens 生產 prodigiosin 之研究 | zh_TW |
dc.title | Studies on the production of prodigiosin by Serratia marcescens | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃健雄,林子清,鍾玉明,蘇南維 | |
dc.subject.keyword | 基因毒性,葡萄糖效應,Serratia marcescens,生產,prodigiosin, | zh_TW |
dc.subject.keyword | glucose effect,gene toxicity,production,prodigiosin,Serratia marcescens, | en |
dc.relation.page | 126 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-01-24 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-94-1.pdf 目前未授權公開取用 | 1.41 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。