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標題: | 微反應器中應用奈米金粒子合成同步檢測DNA Simultaneous DNA Detection with Synthesis of Gold Nanoparticles in Microreactor |
作者: | Sheng-Hung Tsai 蔡昇宏 |
指導教授: | 楊鏡堂(Jing-Tang Yang) |
關鍵字: | 微反應器,二相流,奈米金合成,奈米金堆積,DNA檢測, microreactor,two-phase flow,gold nanoparticle,fouling,DNA detection, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 本文主旨係設計新式二相連續流微反應器,以矽油隔絕水相與流道壁面接觸,避免待測物或產物堆積於微流道壁面;本文也發展新式檢測方法,透過合成奈米金粒子同步檢測DNA,藉由單股與雙股DNA使奈米金合成時產生不同形狀,量測奈米金吸收光譜即可檢測目標DNA。
文中首先探討奈米金於微反應器內的合成;接著為避免奈米金粒子堆積於壁面,設計新式二相微反應器,以矽油隔絕檢測反應與壁面接觸,使用套裝軟體CFD-RC分析,並以實驗觀察流場;接著於二相微反應器內合成奈米金粒子,檢視是否改善堆積狀況;最後,實驗單股DNA與雙股DNA對奈米金合成之影響。 本文所使用之量測工具為分光光譜儀(UV-vis)及穿透式電子顯微鏡(TEM),實驗結果顯示,使用此兩種工具量測粒徑時需確認四氯金酸已完全反應。奈米金合成方面,由於在微反應器中反應時間不夠,使混合較好的微反應器所得粒徑分布較大;但加長微反應器長度會使奈米金全部堆積於微反應器內。為解決堆積問題,本文設計二相微反應器以矽油隔絕奈米金與上下壁面接觸而堆積。模擬結果顯示,當水相流速0.2 m/s,矽油流速0.1 m/s時,矽油可三維包覆於水的周圍,且能夠順利合併;以實驗觀察實際流場發現,確實可形成矽油三維包覆於水的流場,但需使用的水流速比模擬大了約兩倍;而應用於奈米金合成也可發現堆積流道壁面的狀況改善許多。DNA檢測方面,實驗結果顯示未加DNA、加入單股DNA以及加入雙股DNA三種狀況所合成之奈米金吸收光譜有明顯不同,加入單股DNA使吸收光譜產生紅移,加入雙股DNA使紅移現象更為明顯,實驗結果證明本文所提出之方法確實可應用於DNA檢測。 The purpose of this thesis is to design a continuous two-phase microreactor, which applies silicon oil to avoid fouling of gold nanoparticles on channel walls. A new DNA detection method was also devoloped. Gold nanoparticles grow into different shape when single-strand DNA or double-strand DNA is added to growth process. Absorption spectrum was measured to detect target DNA. There are four steps in the thesis. First, gold nanoparticles were synthesized in microreactor. Second, A coutinuous two-phase microreactor was designed to avoid gold nanoparticles fouling on channel walls. This design was analyzed by simulation and experiment. Third, gold nanoparticles were synthesized in the continuous two-phase microreactor. Last, the effects of single-strand DNA and double-strand DNA on synthesis of gold nanoparticles in centrifuge tube were determined. Results are divided into two parts. The first part is continuous two-phase microreactor. The simulation results show that annular flow is formed when the velocity of water is 0.2 m/s and the velocity of water is 0.1 m/s. Vertically straitified flow is formed when the velocity of water increases, while droplet flow is formed when the velocity of water decreases. The experiment results of flow configuration show the similar trend; however, the experimental velocity of water is about two times larger than the simulation to reach the same flow configuration. The continuous two-phase microreactor was then applied to the synthesis of gold nanoparticles. The results show that fouling on channel walls was reduced significantly. The second part is DNA detection. Absorption spectrum of gold nanoparticles synthesized with single-strand DNA has red shift compared with those synthesized without DNA. There is further red shift when gold nanoparticles are synthesized with double-strand DNA. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/39128 |
全文授權: | 有償授權 |
顯示於系所單位: | 機械工程學系 |
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