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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王金和(Ching-Ho Wang) | |
dc.contributor.author | Wan-Hsin Cheng | en |
dc.contributor.author | 鄭宛芯 | zh_TW |
dc.date.accessioned | 2021-06-13T16:46:09Z | - |
dc.date.available | 2005-07-07 | |
dc.date.copyright | 2005-07-07 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-06-28 | |
dc.identifier.citation | 呂榮修。網狀內皮增殖症。禽病診斷彩色圖譜。中華民國養雞協會出版,台北,120-127,1995。
宋華聰、林茂勇。網狀內皮症。禽病檢查手冊。藝軒圖書出版,台北,335-347,1997。 阮意雯。台灣地區家禽白血病病毒之分離與鑑定。國立台灣大學獸醫學研究所。碩士論文。2000。 徐玟良。家禽白血病 J 亞群病毒在台灣土雞及白肉雞的感染情形。台大獸醫學研究所碩士論文,台北,2002。 黃琦新。台灣地區家禽白血病之血清學調查與病毒核酸序列分析。台大獸醫學研究所碩士論文,台北,2001。 陳慧真。台灣土雞家禽白血病J亞群之血清學調查與酵素連結免疫吸附分析 (ELISA) 之開發,台北,2004。 陳秋麟、謝快樂。台灣鵝的網狀內皮增殖病及其病原性研究。台灣省畜牧獸醫學會會報。49: 1-19,1987。 Allen, P. T., J. A. Mullins, C. L. Harris, A. Hellman, R. F. Garry, and M. R. F. Waite. Replication of reticuloendotheliosis virus in mammalian cells (abstract). Amer Soc Microbiol, Annual Meeting. 100:256-256. 1979. Aly, M. M., E. J. Smith, and A. M. Fadly. Detection of reticuloendotheliosis virus infection using the polymerase chain reaction. Avian Pathol. 22:543-554. 1993. Aulisio, C. G., and A. Shelokov. Prevalence of reticuloendotheliosis in chickens: immunofluorescence studies. Proc Soc Exp Biol Med. 130:178-181. 1969. Bagust, T. J. Reticuloendotheliosis virus. In J. B. McFerran, and M. S. mcNulty (eds.). Virus infections of Vertebrates, 4. Virusinfections of Birds, Elsevier Science Publishers B.V.: Amsterdam, 437-454. 1993. Bagust, T. J., and D. P. Dennett. Reticuloendotheliosis virus: experimental infection of poultry and immunofluorescent identification of Australian isolates. Aust. Vet. J. 53:506–508. 1977. Bagust, T. J., and T. M. Grimes. Experimental infection of chickens with an Australian strain of reticuloendotheliosis virus. 2. Serological responses and pathogenesis. Avian Pathol. 8:375-389. 1979. Bagust, T. J., T. M. Grimes, and N. Ratnamohan. Experimental infection of chickens with an Australian strain of reticuloendotheliosis virus. 3. Persistent infection and transmission by the adult hen. Avian Pathol. 10:375-385. 1981. Ban, J., N. L. First, and H. M. Temin. Bovine leukemia virus packaging cell line for retrovirus-mediated gene transfer. J. Gen. Virol. 70:1987-1993. 1989. Barbacid, M., E. Hunter, and S. A. Aaronson. Avian reticuloendotheliosis viruses: evolutionary linkages with mammalian type C retroviruses. J. Virol. 30:508-514. 1979. Barth, M., D. L. Ewert., W. C. Olson, and E. H. Humphries. Reticuloendotheliosis virus REV-T(REV-A)-induced neoplasia: development of tumors within the T-lymphoid and myeloid lineages. J. Virol. 64: 6054-6062. 1990. Bendheim, U. A neoplastic disease in turkeys following fowl pox vaccination. Refu. Vet. 30:35-41. 1973. Borenshtein, R., and I. Davidson. Development of the hot spot–combined PCR assay for detection of retroviral insertions into Marek’s disease virus. J. Virol. Methods. 82:119–127. 1999. Bose, J. H. R. The rel family-models for transcriptional regulation and oncogenic transformation. Biochim. Biophys. Acta. 1114:1-17. 1992. Calvert, J. G., K. Nazerian, R. L. Witter, and N. Yanagida. Fowlpox virus recombinants expressing the envelope glycoprotein of an avian reticuloendotheliosis retrovirus induce neutralizing antibodies and reduce viremia in chickens. J. Virol. 67:3069-3076. 1993. Calvert, J. G., and K. Nazerian. An immunoperoxidase plaque assay for reticuloendotheliosis virus and its application to a sensitive serum neutralization assay. Avian Dis. 38:165-171. 1994. Casella, C. R., A. T. Panganiban. The matrix region is responsible for the differential ability of two retroviruses to function as helpers for vector propagation. Virology. 192:458-464. 1993. Campbell, W. F., K. L. Baxter-Gabbard, and A. S. Levine. Aivan reticuloendotheliosis virus (strain T). I. Virological characterization. Avian Dis. 15:837-849. 1971. Cohen, R. S., T. C. Wong, and M. M. C. Lai. Characterization of thransformation and replication specific sequences of reticuloendotheliosis virus. Virology. 113:672-685. 1981. Crespo, R., P. R. Woolcock., A. M. Fadly, C. Hall, and H. L. Shivaprasad.Characterization of T-cell lymphomas associated with an outbreak of reticuloendotheliosis in turkeys. Avian Pathol. 31:355-61. 2002. Cui, Z. Z., L. F. Lee, R. F. Silva, and R. L. Witter. Monoclonal antibodies against avian reticuloendotheliosis virus: identification of strain-specific and strain-common epitopes. J. Immunol. 136:4237-4242. 1986. Cui, Z. Z., L. F. Lee, E. F. Smith, R. L. Witter, and T. S. Chang. Monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses. Avian Dis. 32:32-40. 1988. Davidson, I., and M. Malkinson. Epidemiology and control of Reticuloendotheliosis virus in chickens and turkeys in Isael. In A. M. Fadly, K. A. Schat, and J. L. Spencer (eds.). Diagnosis and control of neoplastic diseases of poultry, American Association of Avian Pathologists: Kennett Square, PA, 70-75. 1997. Davidson, I., H. Tang, R. L. Witter, and M. Malkinson. The immunodominant proteins of reticuloendotheliosis virus. Vet. Microbiol. 49:273-284. 1996. Delwart E. L., and A. T. Panganiban. Role of reticuloendotheliosis virus envelope glycoprotein in superinfection interference. J. Virol. 63:273-280. 1989. Diallo, I. S., M. A. MacKenzie, P. B. Spradbrow, and W. F. Robinson. Field isolates of fowlpox virus contaminated with reticuloendotheliosis virus. Avian Pathol. 27:60–66. 1998. Dren, C. N., I. Nemeth, I. Sari, F. Ratz, R. Glavtis, and P. Somogyi. Isolation of a reticuloendotheliosis-like virus from naturally occurring lymphoreticular tumours of domestic goose. Avian Pathol. 17:259-277. 1988. Embretson, J. E., and H. M. Temin. Pseudotyped retroviral vectors reveal restrictions to reticuloendotheliosis virus replication in rat cells. J. Virol. 60:662-668. 1986. Embretson, J. E., and H. M. Temin. Transcription from a spleen necrosis virus 5’ long terminal repeat is suppressed in mouse cells. J. Virol. 61:3454-3462. 1987. Fadly, A. M., and R. L. Witter. Comparative evaluation of in vitro and in vivo assays for the detection of reticuloendotheliosis virus as a contaminant in a live vaccine of poultry. Avian Dis. 41:695–701. 1997. Fadly, A. M., and R. L. Witter. Oncoranvirus: leucosis/ sarcomas and reticuloendotheliosis. In: Swayne, D. E., Glisson, J. R., Jackwood, M. W., Pearson, J. E., Reed, W. M., ed. A laboratory manual for the isolation and identification of avian pathogens, 4th ed. Kennett Square, PA: A. A. A. P, 185-196, 1998. Fadly, A. M., R. L. Witter, E. J. Smith, R. F. Silva, W. M. Reed, F. J. Hoerr, and M. R. Putman. An outbreak of lymphomas in commercial broiler breeder chickens vaccinated with a fowlpox vaccine contaminated with reticuloendotheliosis virus. Avian Pathol. 25:35–47. 1996. Fatunmbi, O. O., and W. M. Reed. Evaluation of a commercial modified live virus fowlpox vaccine for the control of “variant” fowl poxvirus infections. Avian Dis. 40:582–587. 1996. Federspiel, M. J., L. B. Crittenden., and S. H. Hughes. Expression of avian reticuloendotheliosis virus envelope confers host resistance. Virology. 173:167-77. 1989. Gautier, R., A. Jiang, V. Rousseau, R. Dornburg, and T. Jaffredo. Avian reticuloendotheliosis virus strain A and spleen necrosis do not infect human cells. J. Virol. 74:518-522. 2000. Garcia, M., N. Narang, W. M. Reed, and A. M. Fadly. Molecular characterization of reticuloendotheliosis virus insertions in the genome of field and vaccine strains of fowl poxvirus. Avian Dis. 47:343-354. 2003. Goff, S. P. Retroviridae: The Retroviruses and their replication. In ”Fields Virology” ,4th ed., pp. 1871-1939. Lippincott-Raven, Philadelphia, PA. 2001. Grimes, T. M. and H. G. Purchase. Reticuloendotheliosisin a duck. Aust. Vet. J. 49:466-471. 1973. Hertig, C., B. E. H. Coupar, A. R. Gould, and D. B. Boyle. Field and vaccine strains of fowlpox virus carry integrated sequences from the avian retrovirus, reticuloendotheliosis virus. Virology. 235:367–376. 1997. Hussain, A. I., J. A. Johnson, M. Da Silva Freire, and W. Heneine. Identification and characterization of avian retroviruses in chicken embryo-derived yellow fever vaccines: investigation of transmission to vaccine recipients. J. Virol. 77:1105-1111. 2003. Ianconescu, M. Reticuloendotheliosis antigen for the agar gel precipitation test. Avian Pathol. 6: 259-267. 1977 Jackson, C. A. W., S. E. Dunn, D. I. Smith, P. T. Gilchrist, and P. A. Mcqueen. Proventriculitis “Nakanuko” and reticuloendotheliosis in chickens following vaccination with herpesvirus of turkeys (HVT). Aust. Vet. J. 53:457–459. 1977. Kawamura, H., T. Wakabayashi, S. Yamaguchi, T. Taniguchi, N. Tarayanagi, S. Sato, S. Sekiya, and T. Horiuchi. Inoculation experiment of Marek's disease vaccine contaminated with reticuloendotheliosis virus. Natl. Inst. Anim. Health Q. 16:135–140. 1976. Keshet, E. and H. M. Temin. Cell killing by spleen necrosis virus is correlated with a transient accumulation of spleen necrosis virus DNA. J. Virol. 31:376-388. 1979. Kim, T. J., and D. N. Tripathy. Reticuloendotheliosis virus integration in the fowl poxvirus genome: not a recent event. Avian Dis. 45:663–669. 2001. Koyama, H., Y. Suzuki, Y. Ohwada, and Y. Saito. Reticuloendotheliosis group virus pathogenic to chicken isolated material infected with turkey herpesvirus (HVT). Avian Dis. 20:429–434. 1976. Larose, R.N., and M. Sevoian. Avian lymphomatosis. IX. Mortality and serological response of chickens of various ages to graded doses of T strain. Avian Dis. 9:604-610. 1965. Li, J., B. W. Calnek, K. A. Schat, and D. L. Graham. Pathogenesis of reticuloendotheliosis virus infection in ducks. Avian Dis. 27:1090-1105. 1983 Ludford, C. G., H. G. purchase, and H. W. Cow. Duck infectious anemia virus associated with plasmodium lophurae. Exp. Parasitol. 31:29-38. 1972. Martinez, I. and R. Dornburg. Mutational analysis of the envelope protein of spleen necrosis virus. J. Virol. 70:6036-6043. 1996. Martinez, I. and R. Dornburg. Mapping of receptor binding domains in the envelope protein of spleen necrosis virus. J. Virol. 69:4339-4346. 1995. McDougall, J. S., P. M. Biggs, and R. F. W. Shilleto. A leucosis in turkeys associated with infection with reticuloendotheliosis virus. Avian Pathol. 7:557-598. 1978. McDougall, J. S., R. F. W. Shilleto, and P. M. Biggs. Experimental infection and vertical transmission of reticuloendotheliosis virus in the turkey. Avian Pathol. 9:445-454. 1980. Meyers, N. L. Antibody response elicited against empty reticuloendotheliosis virus particles in two inbred lines of chicken. Vet. Microbiol. 36:317-332. 1993. Moore, K. M., J. R. Davis, T. Sato, and A. Yasuda. Reticuloendotheliosis virus (REV) long terminal repeats incorporated in the genomes of commercial fowlpox vaccines and pigeon poxviruses without indication of the presence of infectious REV. Avian Dis. 44:827–841. 2000. Motha, M. X. J. Distribution of virus and tumour formation in ducks experimentally infected with reticuloendotheliosis virus. Avian Pathol. 13:303-320. 1984. Motha, M. X. J. Clinical effects, virological and serological responses in chickens following in-ovo inoculation of reticuloendotheliosis virus. Vet. Microbiol. 14:411-417. 1987. Motha, M. X. J. Demonstration of precipitating antibodies to reticuloendotheliosis virus in egg yolk. Aust. Vet. J. 64: 259-260. 1987. Motha, M. X. J., and J. R.Egerton. Vertical transmission of reticuloendotheliosis virus in chickens. Avian pathol. 16:141-148. 1987. Motha, M. X. J., J. R. Egerton, and A. W. Sweeney. Some evidence of mechanical transmission of Reticuloendotheliosis virus by mosquitos. Avian Dis. 28:858-867. 1984. Mussman, H. C., and M. J. Twiehaus. Pathogenesis of reticuloendothelial virus disease in chicks-an acute runting syndrome. Avian Dis. 15:483-502. 1971. Nazerian, K., J. G.. Calvert, R.L. Witter, and N. Yanagida. Vaccine comprising fowlpox virus recombinants expressing the envelope glycoprotein of an avain reticuloendotheliosis retrovirus. US Patent Office#540358. 1995. Nicohlas, R. A. J., and D. H. Thornton. An enzyme-linked immunosorbent assay for the detection of antibodies to avian reticuloendotheliosis virus using whole cell antigen. Res. Vet. Sci. 43:403-404. 1987. Nicohlas, R. A. J., and D. H. Thornton. Relative efficiency of techniques for detecting avian reticuloendotheliosis virus as a vaccine contaminant. Res. Vet. Sci. 34:377-379. 1983. Paul, P. S., K. H. Johnson, K. A. Pomeroy, B. S. Pomeroy, and P. S. Sarma. Experimental transmission of reticuloendotheliosis in turkeys with the cell-culture-propagated reticuloendotheliosis viruses of turkey origin. J Natl. Can. Inst. 58:1819-1824. 1977. Paul, P. S., K. A. Pomeroy, P. S. Sarma, K. H. Johnson, D. M. Barnes, M. C. Kumar, and B. S. Pomeroy. Brief communication: naturally occurring reticuloendotheliosis in turkeys: transmission. J Natl. Can. Inst. 56:419-421. 1976. Paul, P. S., and R. W. Werdin. Spontaneously occurring lymphoproliferative disease in ducks (case reports). Avian Dis. 22:191-195. 1978. Payne, L. N. Retrovirus induced disease in poultry. Poult Sci. 77:1204-1212. 1998. Perk, K., M. Malkinson, A. Gazit, A. Yaniv, and A. Zimber. Reappearance of an acute undifferentiated leukemia in a flock of Muscovy ducks. Proceedings of the 10th International Symposium on Comparative Leukemia and Related Diseases. 99-100. 1981. Purchase, H. G., C. G. Luford, K. Nazerian., and H. W. Cox. A new group of oncogenic viruses: reticuloendotheliosis, chick syncytial, duck infectious anemia, and spleen necrosis viruses. J. Natl. Cancer Inst. 51:489-499. 1973. Purchase, H. G., and R. L. Witter. The reticuloendotheliosis viruses. Microbiol. Immunol. 71:103-124. 1975. Rehacek, J. T. Dolan, K. Thompson, R. G. Fischer, Z. Rehacek, H. Johnson. Cultivation of oncogenic viruses in mosquito cells in vitro. Curr Top Microbiol Immunol. 55:161-164. 1971. Robinson, F. R., and M. J. Twiehaus. Isoaltion of the avian reticuloendotheliosis virus (strain T). Avian Dis. 18:278-288. 1974. Rosner, B. Fundamentals of bioststistics. 5th edition, Duxbury. 2000. Rushlow, C., and R. Warrior. The rel family of proteins. Bioessays. 14:89-95. 1992. Salter, D. W., E. J. Smith, S. H. Hughes, S. E. Wright, and L. B. Crittenden. Transgenic chickens: Insertion of retroviral genes into the chicken germ line. Virology. 157:236-240. 1986 Sevoian, M. R., R. N. Larose, and D. M. Chamberlain. Avian lymphomatosis. VI. A virus of unusual potency and pathogenicity. Avian Dis. 3:336-347. 1964. Shimotohno, K., S. Mizutani, and H. M. Termin. Sequence of retrovirus provirus resembles that of bacterial transposable elements. Nature. 285:550-554. 1980. Silva R. F. Differentiation of pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome. Avian Dis. 36:521-528. 1992. Simek, S., and N. R. Rice. Analysis of the nucleic acid components in reticuloendotheliosis virus. J. Virol. 33:320-329. 1980. Singh, P., T.-J. Kim, and D. N. Tripathy. Re-emerging fowlpox: evaluation of isolates from vaccinated flocks. Avian Pathol. 29:449–455. 2000. Singh, P., W. M. Schnitzlein, and D. N. Tripathy. Reticuloendotheliosis virus sequences within the genomes of field strains of fowlpox virus display variability. J. Virol. 77:5855-5862. 2003 Singh, P., and D. N. Tripathy. Characterization of monoclonal antibodies against fowl poxvirus. Avian Dis. 44:365-371. 2000. Smith, E. J., and R. L. Witter. Detection of antibodies against reticuloendotheliosis viruses by an enzyme-linked immunosorbent assay. Avian Dis. 27: 225-234. 1983. Theodros, T. and M. R. Willie. Detection of specific reticuloendotheliosis virus sequence and protein from REV-integrated fowlpox virus strains. J. Virol. Methods. 110: 99-104. 2003 Theilen, G. H, R. F. Zeigel, and M. J. Twiehaus. Biological studies with RE virus (strain T) that induces reticuloendotheliosis in turkeys, chickens, and Japanese quail. J Natl Cancer Inst. 37: 731-743. 1966. Trager, W. A new virus of ducks interfering with development of malaria parasite (Plasmodium lophurae). Proceedings of the Society for Experimental Biology and Medicine. 101:578-582. 1959. Tsai, W. P., T. D. Copeland, and S. Oroszlan. Purification and chemical and immunological characterization of avian reticuloendotheliosis virus gag-gene-coded structural proteins. Virology. 140:289-312. 1985. Tsai, W. P., T. D. Copeland, and S. Oroszlan. Biosynthesis and chemical and immunological characterization of avian reticuloendotheliosis virus env gene-coded proteins. Virology. 155:567-583. 1986. Tsai, W. P., and S. Oroszlan. Novel glycosylation pathways of retroviral envelope proteins identified with avian reticuloendotheliosis virus. J. Virol. 62:3167-74. 1988. Tsai, W. P., and S. Oroszlan. Site-directed cytotoxic antibody against the c-terminal segment of the surface glycoprotein gp90 of avian reticuloendotheliosis virus. Virology. 166:608-611. 1988. Wakabayashi, T., and H. Kawamura. Virus of endotheliosis virus group: Persistent infection in chickens and viral transmission to fertile egg. Proc 79th Ann Mtg Jap Soc Vet Sci. 12-13. 1975. Wakabayashi, T., and H. Kawamura. Serological survey of reticuloendotheliosis virus infection among chickens in Japan. Natl. Inst. Anim. Health Q. 17:73-74. 1977. Watanabes, S., and H. M. Temin. Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene. Proc Natl Acad Sci U S A. 79:5986-5990. 1982. Watanabes, S., and H. M. Temin. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol. Cell. Biol. 3:2241-2249. 1983. Weaver, T. A., K. J. Talbot, and A. T. Panganiban. Spleen necrosis virus gag polyprotein is necessary for particle assembly and release but not for proteolytic processing. J. Virol. 64:2642-2652. 1990. Witter, R. L., and A. M. Fadly. Reticuloendotheliosis. In: Diseases of poultry, 11th ed. Saif, Y. M. et al. eds. Amer. Assoc. Avian Pathol., Iowa State Press, a Blackwell Publishing Co., Ames, IA. pp. 517-536. 2003. Witter, R. L., and D. C. Johnson. Epidemiology of reticuloendotheliosis virus in broiler breeder flocks. Avian Dis. 29:1140-1154. 1985. Witter, R. L., L. F. Lee, L. D. Bacon, and E. J. Smith. Depression of vaccinal immunity to Marek’s disease by infection with reticuloendotheliosis virus. Infect. Immun. 26:90-98. 1979. Witter, R. L., J. M. Sharma, and A. M. Fadly. Nonbursal lymphomas induced by nondefective reticulendotheliosis virus. Avian Pathol.15:467-486.1986. Witter, R. L. and D. W. Salter. Vertical transmission of reticuloendotheliosis virus in breeder turkeys. Avian Dis.33:226-235. 1989. Witter, R. L., I. L. Peterson, E. J. Smith, and D. C. Johnson. Serologic evidence in commercial chicken and turkey flocks of infection with Reticuloendotheliosis virus. Aivan Dis. 26:753-762. 1982. Witter, R. L., H. G. Purchase, and G.. H. Burgoyne. Peripheral nerve lesions similar to those of Marek’s disease in chickens inoculated with reticuloendotheliosis virus. J. Natl. Cancer Inst. 45:567-577. 1970. Witter, R. L., E. J. Smith, and L. B. Crittenden. Tolerance, viral shedding, and neoplasia in chickens infected with non-defective reticuloendotheliosis viruses. Avian Dis. 25:374-394. 1981. Witter, R. L., D. Li, D. Jones, L. F. Lee, and H. J. Kung. Retroviral insertional mutagenesis of a herpesvirus: a Marek's disease virus mutant attenuated for oncogenicity but not for immunosuppression or in vivo replication. Avian Dis. 41:407-421. 1997. Wong, T. C., and M. M. C. Lai. Avian reticuloendotheliosis virus contains a new class of oncogene of turkey origin. Virology. 111:289-293. 1981. Yang, H. C., and Y. Gao. Detection of antibody to reticuloendotheliosis virus in sera by a modified blocking ELISA. Chin. J. Vet. Med. 23:3-5. 1997. Yuasa, N., I. Yoshida, and I. Taniguchi. Isolation of a reticuloendotheliosis virus from chickens inoculated with Marek's disease vaccine. Natl. Inst. Anim. Health Q. 16:141–151. 1976. Zhang, J., and J. H. R. Bose. Acquisition of new proviral copies in avian lymphoid cells transformed by reticuloendotheliosis virus. J. Virol. 63:1107-15. 1989. Zhu, G.. S., T. Ojima, T. Hironaka, T. Ihara, N. Mizukoshi, A. Kato, S. Ueda, K. Hirai. Differentiation of oncogenic and nononcogenic strains of Marek's disease virus type 1 by using polymerase chain reaction DNA amplification. Avian Dis. 36:637-645. 1992. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38785 | - |
dc.description.abstract | 家禽網狀內皮症病毒 (avian reticuloendotheliosis virus, REV),在台灣家禽疾病中較不被重視,但現場感染雞隻可發現雞隻出現生長不良、羽毛生長異常、胸腺及華氏囊萎縮等症狀發生,但是尚無有效的預防控制方法,所以目前種雞場最重要的是區分感染及未感染的雞隻,以避免病毒傳播的可能;並檢測現場使用之疫苗是否有REV污染,以釐清現今台灣所使用的疫苗在病毒傳播上所扮演的角色。因此本研究以ELISA方式調查台灣種雞及土雞REV抗體陽性率,同時由血液中抽取DNA檢測病毒核酸,並由臨床送檢病例的血液樣本與腫瘤乳劑中進行病毒分離,此外也從動物用藥品檢定分所取得禽痘疫苗與馬力克疫苗以進行REV污染檢測。在抗體陽性率結果顯示在種雞場之抗體陽性率為83%,土雞場之抗體陽性率為8%,而病毒核酸檢測結果在土雞的感染率為29%,由結果可知台灣雞隻REV感染情形相當普遍。病毒分離方面由現場病例中分離到兩株病毒,編號分別為3122/03與3295/04。而疫苗檢測結果方面,則從18種禽痘病毒疫苗中分離出一株且此分離株具有細胞病變 (cytopathic effect, CPE) 現象;在馬立克病毒疫苗方面,17種疫苗都未分離到REV病毒。由於目前商用ELISA套組檢測方式以偵測抗體為主,所以本研究欲想發展偵測抗原之ELISA,以便現場診斷之大量運用,所以本研究則利用REV分離株3122的部分envelope基因選殖出來,進行重組蛋白3122env的表現並針對選殖部分的胺基酸片段發展單株抗體,結果共獲得3株單株抗體mAb1、mAb2及mAb3,進一步利用單株抗體來偵測REV抗原,結果可順利偵測到重組蛋白與REV病毒,顯示這些單株抗體具有應用在偵測抗原型ELISA之潛力。綜和本實驗之結果,由血清學、病毒核酸檢測及病毒分離方面,皆充分顯示出台灣REV感染情形相當普遍,此外亦從疫苗株分離到REV,更代表此疾病在雞群中之潛在危險性。 | zh_TW |
dc.description.abstract | Avian reticuloendotheliosis virus (REV) infection is common, but not ubiquitous. The REV-infected chickens showed stunted, pale, and abnormal feather development. The principal lesions include runting, and atrophy of the thymus and bursa of Fabricius. No procedures have been applied in commercial practice for the control of RE, so the most important thing in the field is to distinguish the infected and uninfected chickens. In this study, we used commercial ELISA kits to detect REV-specific antibodies in color-breed chickens, breeder chickens, and broilers; meanwhile we detected the viral DNA in blood by polymerase chain reaction (PCR). Clinical materials, including whole blood, plasma, tumor tissues, and litter, were collected for virus isolation. We collected fowl pox virus (FPV) vaccines and Marek’s disease (MDV) vaccines provided by branch institute of animal drugs inspection animal health research institute for detection of REV contamination, and tried to isolate REV from these vaccines. The result of prevalence investigation showed 83% antibody positive rate in breeder chickens and 8% in color-breed chickens. The result of detection of REV in native color hybrid blood samples by PCR showed 29% positive rate, which meant that the infection of REV was popular in Taiwan. Two REVs, named 3122/03 and 3295/04, were isolated from clinical materials. Among eighteen FPV vaccines we collected, eleven of them showed REV LTR sequences integration and one REV was isolated from these vaccines. This isolate had cytopathic effect in DF1 cell line. No REV was isolated from seventeen MDV vaccines. We cloned partial envelope gene for the expression of recombinant protein used for the production of monoclonal antibody to develop antigen-capture ELISA. The results showed that we had selected three monoclonal antibodies which could recognize the recombinant protein and REVs. These monoclonal antibodies have potential for developing of antigen-capture ELISA. Based on the results of serology, REV DNA detection, and virus isolation, the REV infection in Taiwan is common. Specially, the isolation of REV from FPV vaccines shows a potential danger in the field. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T16:46:09Z (GMT). No. of bitstreams: 1 ntu-94-R92629019-1.pdf: 2128047 bytes, checksum: d67bd9b903744c7714b3ed2e9cd2eec8 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄
目錄----------------------------------------------------------------------------------------------Ⅰ 表次----------------------------------------------------------------------------------------------Ⅶ 圖次----------------------------------------------------------------------------------------------Ⅷ 附錄----------------------------------------------------------------------------------------------Ⅹ 中文摘要-------------------------------------------------------------------------------------ⅩⅠ 英文摘要-------------------------------------------------------------------------------------ⅩⅡ 第一章 緒言-------------------------------------------------------------------------------------1 第二章 文獻探討-------------------------------------------------------------------------------3 2.1 歷史背景------------------------------------------------------------------------------------3 2.2 家禽網狀內皮症病毒介紹---------------------------------------------------------------3 2.3 病毒分類------------------------------------------------------------------------------------3 2.4 病毒型態與特性---------------------------------------------------------------------------4 2.5 致癌基因------------------------------------------------------------------------------------4 2.6 家禽網狀內皮症病毒基因結構與病毒蛋白------------------------------------------5 2.7 家禽網狀內皮症病毒之宿主特異性---------------------------------------------------5 2.7.1 病毒複製之宿主特異性----------------------------------------------------------------5 2.7.2 自然與實驗感染宿主-------------------------------------------------------------------6 2.8 病毒傳播------------------------------------------------------------------------------------6 2.8.1 水平傳播----------------------------------------------------------------------------------6 2.8.2 垂直傳播----------------------------------------------------------------------------------7 2.8.3 污染的生物性材料----------------------------------------------------------------------8 2.9 潛伏期---------------------------------------------------------------------------------------8 2.10 臨床症狀-----------------------------------------------------------------------------------9 2.11免疫抑制-----------------------------------------------------------------------------------10 2.12 病理變化----------------------------------------------------------------------------------10 2.13 血清學-------------------------------------------------------------------------------------10 2.14 家禽網狀內皮症病毒之實驗室診斷-------------------------------------------------11 2.15 區別診斷----------------------------------------------------------------------------------11 2.16 家禽網狀內皮症病毒污染活毒疫苗及插入FPV野外分離株基因中的情形12 2.17 家禽網狀內皮症病毒污染MDV活毒疫苗的情形--------------------------------14 2.18 家禽網狀內皮症病毒之免疫與治療-------------------------------------------------15 2.19 家禽網狀內皮症病毒之預防與控制-------------------------------------------------15 第三章 材料與方法---------------------------------------------------------------------------16 第一節 血清學調查---------------------------------------------------------------------------16 3.1.1血漿樣本採集----------------------------------------------------------------------------16 3.1.1.1 採樣方式-------------------------------------------------------------------------------16 3.1.1.2 採樣來源-------------------------------------------------------------------------------16 3.1.2 血清學檢測------------------------------------------------------------------------------16 3.1.2.1 抗體檢測-------------------------------------------------------------------------------16 3.1.2.2 結果判定-------------------------------------------------------------------------------17 第二節 屠宰場有色雞群血液樣本之REV病毒核酸檢測-----------------------------17 3.2.1 血液樣本採集---------------------------------------------------------------------------17 3.2.1.1 採樣方式-------------------------------------------------------------------------------17 3.2.1.2 採樣來源-------------------------------------------------------------------------------17 3.2.2 血液之DNA萃取-----------------------------------------------------------------------17 3.2.3 聚合酶鏈鎖反應------------------------------------------------------------------------18 第三節 病毒分離------------------------------------------------------------------------------19 3.3.1 病材採樣---------------------------------------------------------------------------------19 3.3.1.1 血液樣本之採樣----------------------------------------------------------------------19 3.3.1.2 腫瘤組織之採樣----------------------------------------------------------------------19 3.3.2 細胞培養---------------------------------------------------------------------------------20 3.3.3 冷凍與解凍細胞------------------------------------------------------------------------20 3.3.4 病毒分離---------------------------------------------------------------------------------21 3.3.5 病毒偵測---------------------------------------------------------------------------------21 3.3.5.1 血液之DNA萃取--------------------------------------------------------------------21 3.3.5.2 細胞之DNA萃取--------------------------------------------------------------------21 3.3.5.3 聚合酵素鏈反應 --------------------------------------------------------------------22 3.3.5.4 病毒RNA之萃取---------------------------------------------------------------------22 3.3.5.5 反轉錄聚合酵素鏈反應 -----------------------------------------------------------22 3.3.6 聚合酶鏈反應產物之選殖 (cloning)-----------------------------------------------23 3.3.6.1 聚合酶鏈反應產物之純化----------------------------------------------------------23 3.3.6.2 聚合酵素鏈反應產物之選殖-------------------------------------------------------24 3.3.7 免疫螢光分析 (Immunofluorescence assay, IFA)---------------------------------24 3.3.8 病毒力價測定---------------------------------------------------------------------------25 3.3.9 病毒中和試驗 (Virus neutralization test)-------------------------------------------26 3.3.10 病毒之濃縮與純化-------------------------------------------------------------------26 3.3.11 電子顯微鏡之病毒型態-------------------------------------------------------------27 3.3.12 蛋白質之定量-------------------------------------------------------------------------27 3.3.13 病毒核酸序列的定序及分析-------------------------------------------------------27 3.3.13.1 核酸序列的定序--------------------------------------------------------------------27 3.3.13.2 序列之分析--------------------------------------------------------------------------28 第四節 疫苗中REV污染檢測及自疫苗中病毒分離-----------------------------------28 3.4.1疫苗來源----------------------------------------------------------------------------------28 3.4.2禽痘疫苗之病毒分離-------------------------------------------------------------------28 3.4.3禽痘疫苗之DNA萃取-----------------------------------------------------------------29 3.4.4馬立克疫苗之病毒分離----------------------------------------------------------------29 3.4.5 馬立克疫苗之DNA萃取--------------------------------------------------------------29 3.4.6聚合酵素鏈反應檢測-------------------------------------------------------------------29 3.4.7病毒RNA之萃取------------------------------------------------------------------------30 3.4.8反轉錄聚合酵素鏈反應----------------------------------------------------------------30 3.4.9巢式聚合酵素鏈反應 (Nest-PCR)---------------------------------------------------31 第五節 單株抗體------------------------------------------------------------------------------31 3.5.1 單株抗體之製備------------------------------------------------------------------------31 3.5.1.1封套基因選殖--------------------------------------------------------------------------31 3.5.1.2 建構表現載體-------------------------------------------------------------------------32 3.5.1.3 免疫計畫-------------------------------------------------------------------------------32 3.5.1.4 單株抗體篩選-------------------------------------------------------------------------33 3.5.1.5 表現質體之保存----------------------------------------------------------------------33 3.5.2 重組蛋白之表現------------------------------------------------------------------------34 3.5.2.1重組蛋白之確認-----------------------------------------------------------------------34 3.5.3單株抗體之確認與應用----------------------------------------------------------------35 3.5.3.1單株抗體之確認-----------------------------------------------------------------------35 3.5.3.1.1 免疫螢光分析 (Immunofluorescence assay, IFA) ----------------------------35 3.5.3.1.2 SDS PAGE及西方墨點法 (Western blotting)-----------------------------35 3.5.3.1.3 免疫墨點法 (Immunodot blot assay)-------------------------------------------36 3.5.3.1.4 病毒中和試驗 (Virus neutralization test) -------------------------------------36 第四章 結果------------------------------------------------------------------------------------37 第一節 血清學調查--------------------------------------------------37 4.1.1 台灣種雞群之血清學調查------------------------------------------------------------37 4.1.2 台灣有色雞群之血清學調查---------------------------------------------------------37 第二節 REV抗原檢測------------------------------------------------------------------------37 4.2.1屠宰場有色雞群血液樣本之REV病毒核酸檢測---------------------------------37 第三節 病毒分離與病毒偵測---------------------------------------------------------------38 4.3.1 病材採樣---------------------------------------------------------------------------------38 4.3.1.1 病例介紹-2003年至2004年現場病例之家禽網狀內皮症病毒分離情形----------------------------------------------------------------------------------------------------38 4.3.2 病毒來源---------------------------------------------------------------------------------39 4.3.3 聚合酵素鏈鎖反應---------------------------------------------------------------------40 4.3.4 反轉錄聚合酵素鏈鎖反應------------------------------------------------------------40 4.3.5 聚合酵素鏈反應產物之選殖 (cloning)--------------------------------------------40 4.3.6 病毒力價測定---------------------------------------------------------------------------40 4.3.7 病毒中和試驗 -------------------------------------------------------------------------40 4.3.8 病毒之濃縮與純化---------------------------------------------------------------------41 4.3.9 免疫螢光分析---------------------------------------------------------------------------41 4.3.10 電子顯微鏡之病毒型態-------------------------------------------------------------41 4.3.11 蛋白質之定量--------------------------------------------------------------------------41 4.3.12 序列分析-------------------------------------------------------------------------------42 4.3.13免疫墨點法-----------------------------------------------------------------------------42 4.3.14 SDS PAGE及西方墨點法------------------------------------------------------------42 第四節 疫苗檢測------------------------------------------------------------------------------42 4.4.1 禽痘疫苗檢測---------------------------------------------------------------------------42 4.4.2 馬立克疫苗檢測------------------------------------------------------------------------43 4.4.3 序列分析---------------------------------------------------------------------------------43 第五節 單株抗體------------------------------------------------------------------------------43 4.5.1 重組蛋白之表現------------------------------------------------------------------------43 4.5.1.1 重組蛋白之確認----------------------------------------------------------------------43 4.5.2 單株抗體之確認------------------------------------------------------------------------43 4.5.2.1免疫螢光分析 ------------------------------------------------------------------------44 4.5.2.2 免疫墨點法 --------------------------------------------------------------------------44 4.5.2.3 SDS PAGE及西方墨點法 ---------------------------------------------------------44 4.5.2.4 病毒中和試驗 ----------------------------------------------------------------------44 第五章 討論與結論---------------------------------------------------------------------------46 第六章 參考文獻------------------------------------------------------------------------------53 表次 Table 1: Serological investigation of reticuloendotheliosis antibody positive rate from different breeds of chickens during 2003 to 2005---------------------------------------64 Table 2: Positive rates of polymerase chain reaction for reticuloendotheliosis virus in broiler breeder and native color hybrid blood samples collected during 2004----- 64 Table 3: Reticuloendotheliosis virus has being isolated from these clinical cases and antibody positive rates of different flocks at Yilan in November, 2004--------------65 Table 4: Antibody positive rates of different flocks in 3273 chicken farms at Miaoli in August, 2004---------------------------------------------------------------------------------65 Table 5: Reticuloendotheliosis virus has being isolated from these clinical cases and antibody positive rates of different flocks in 3295-3299 chicken farms at Changhua in September, 2004--------------------------------------------------------------------------66 Table 6: Detection of reticuloendotheliosis virus (REV) integrated in fowl pox vaccines by polymerase chain reaction and virus isolation ---------------------------67 Table 7: Detection of reticuloendotheliosis virus (REV) integrated in Marek’s disease vaccines by polymerase chain reaction and virus isolation ---------------------------68 Table 8: Titer of 3 isolates of reticuloendotheliosis virus propagated in chicken fibroblast as detected by polymerase chain reaction and immunofluorescence assay -------------------------------------------------------------------------------------------------69 Table 9: Western blotting analysis of concentrated antigens (isolate 3122/03, 3295/04, 3134/03) and of recombinant protein (3122/03 env protein) recognized with anti-reticuloendotheliosis virus antiserum (Charles River), mAb1, mAb2, and mAb3------------------------------------------------------------------------------------------69 Table 10: Detection of reticuloendotheliosis virus (REV) against different antibodies -------------------------------------------------------------------------------------------------70圖次 Figure 1: Schematic diagram of a retrovirus virion indicating various structures and proteins----------------------------------------------------------------------------------------71 Figure 2: The structure of the 9.0 Kb genome of reticuloendotheliosis virus A strain (REV-A; helper virus)----------------------------------------------------------------------72 Figure 3: The structure of the 5.7 Kb genome of reticuloendotheliosis virus T strain (REV-T; defective virus)-------------------------------------------------------------------72 Figure 4: Schematic representation of reticuloendotheliosis virus (REV) provirus integrated into the genomes of fowl poxvirus (FPV) field and vaccine strain viruses. (A) as were as of the long (B) and short (C) REV LTR remnants present in the FPV genome are shown---------------------------------------------------------------------------73 Figure 5: Maps of the env5/env6、env1/env2、rel8/rel4 primers for polymerase chain reaction ---------------------------------------------------------------------------------------73 Figure 6: Seroprevalence of reticuloendotheliosis virus at different ages and different breed-------------------------------------------------------------------------------------------74 Figure 7: Reverse transcriptase polymerase chain reaction analysis of the reticuloendotheliosis virus (REV)---------------------------------------------------------75 Figure 8: Detection of isolate 3122/03 from clinical signs in case 3121 to 3126 by immunofluorescence assay ----------------------------------------------------------------76 Figure 9: Purification of the isolate 3122/03 of reticuloendotheliosis virus (REV) for mmunodot blot assay, and western blot --------------------------------------------------77 Figure10: Negative stain electron micrograph of reticuloendotheliosis virus isolate 3295/04----------------------------------------------------------------------------------------78 Figure 11: Virus titration (TCID50) of REV isolates by PCR-- --------------------------79 Figure 12: DF1 cells infected with isolate 3134/03 of reticuloendotheliosis virus----80 Figure 13: Detection of isolate 3134/03 from fowl poxvirus vaccine by immunofluorescence assay---------------------------------------------------------------- 81 Figure 14: (A) SDS-PAGE analysis of different purification virus and expressed protein (B) Western blotting profile of isolate reticuloendotheliosis virus obtained following incubation of the nitrocellulose membrane with polyclonal antibody (Charles River)-------------------------------------------------------------------------------82 Figure 15: TA cloning of reticuloendotheliosis virus (REV) envelope gene-----------83 Figure 16: The envelope gene sequence of isolate 3122/03------------------------------84 Figure 17: The envelope gene sequence of isolate 3295/05------------------------------85 Figure 18: The partial envelope gene sequence of isolate 3134/03----------------------86 Figure 19: Percentage identity and divergence of the nucleotide sequences (nt. 22 – 1455) of the envelope gene of reticuloendotheliosis virus (REV) isolates 3122/03, 3134/03, 3292/ 05, and published sequences -------------------------------------------87 Figure 20: Phylogenetic tree of the nucleotide sequence of the envelope gene of reticuloendotheliosis virus (REV) isolates 3122/03, 3134/03, 3295/05, and published sequences-------------------------------------------------------------------------88 Figure 21: SDS-PAGE analysis of E. coli-expressed protein. E. coli BL21 (LysE) cells with pET 3122/03 env.----------------------------------------------------------------------89 Figure 22: Immunodot blot assay by using monoclonal antibodies 1, 2 and 3 and polyclonal antibodies (Charles River)----------------------------------------------------90 Figure 23: Western blotting assay using monoclonal antibodies 1, 2, 3, and polyclonal antibodies (Charles River)------------------------------------------------------------------91 Figure 24: Polymerase chain reaction analysis in the Marek’s disease (MD) vaccine92 Figure 25: Polymerase chain reaction analysis of the fowl poxvirus vaccine ---------93 Figure 26: Fluorescent micrograph of isolate 3295/04 of reticuloendotheliosis virus infected DF1 reacted with monoclonal antibody 2 (magnification 400x)-----------94附錄 Appendix 1. 95% 的機率至少可以檢出一個陽性個體所需的樣本數---------------95 Appendix 2: The prevalence of reticuloendotheliosis antibody in layer breeder chickens in 2004.----------------------------------------------------------------------------96 Appendix 3: The prevalence of reticuloendotheliosis antibody in broiler breeder chickens---------------------------------------------------------------------------------------97 Appendix 4: The prevalence of reticuloendotheliosis antibody in colored breeder chickens.--------------------------------------------------------------------------------------98 Appendix 5: Serological investigation of reticuloendotheliosis virus in native color hybrid chicken sera collected from the different slaughter house.-----------------------99 Appendix 6: IDEXX data--------------------------------------------------------------------100 Appendix 7: Detection of LTR-region of REV in colored chicken from a slaughter house by PCR.------------------------------------------------------------------------------101 Appendix 8: Positive values (S/P value) of REV antibody positive serum samples from clinical cases 3121 to 3126.--------------------------------------------------------102 Appendix 9. Solution formula used in this study-----------------------------------------103 Appendix 10. 中英對照縮寫------------------------------------------105 | |
dc.language.iso | zh-TW | |
dc.title | 台灣家禽網狀內皮症病毒分離鑑定與序列分析 | zh_TW |
dc.title | Isolation, Identification and Sequence Analysis of Avian Reticuloendotheliosis Viruses in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林茂勇(Maw-Yeong Lin),連一洋(Yi-Yang Lien),劉宏仁(Hung-Jen Liu) | |
dc.subject.keyword | 家禽網狀內皮症病毒,封套基因, | zh_TW |
dc.subject.keyword | avian reticuloendotheliosis virus,envelope gene, | en |
dc.relation.page | 105 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-06-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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