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  1. NTU Theses and Dissertations Repository
  2. 生物資源暨農學院
  3. 獸醫專業學院
  4. 獸醫學系
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38642
完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor張本恆
dc.contributor.authorMi-Ching Yangen
dc.contributor.author楊彌青zh_TW
dc.date.accessioned2021-06-13T16:40:16Z-
dc.date.available2006-07-08
dc.date.copyright2005-07-08
dc.date.issued2005
dc.date.submitted2005-07-04
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?性流行病的病原及病理。 廈門大學學報 40:949-957,2001。

曲敬正。九孔(Haliotis diversicolor supertexta Lischke)繁養殖之基礎研
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曾文陽 。 鮑魚養殖學。台北市,1985。

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王軍、蘇永全、張蕉南、黃英、張朝霞、皺慶枇、王德祥。春季東山九
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蕭終融等編。獸醫臨床細菌學檢驗手冊。1994。

鄭穹翔。電子顯微鏡應用於動物疾病之診斷。國立台灣大學獸醫學系。
2003年動物疫病論斷鑑定技術研習會專輯。23-29,2003。

Andree KB, Friedman CS, Moore JD, Hedrick RP. A polymerase chain
reaction for detection of genomic DNA of a Rickettsiales-like
prokaryote associated with Withering Syndrome in Black Abalone
(Haliotis cracherodii). J Shellfish Res 19: 213-218, 2000.

Botes H, Basson L, Van As LL. Digenean trematodes found associated with
Haliotis spadicea Donovan, 1808. Proceeding of the Microscopy Society
of Southern Africa 29: 76-77, 1999.

Bower SM. Labyrinthuloides haliotidis n. sp.(Protozoa: Labyrinthomorpha),
a pathogenic parasite of small juvenile abalone in a British Columbia
mariculture facility. Can J Zool 65: 1996-2007, 1987.

Caceres-Martinez J, Tinoco-Orta GD. Symbionts of cultured red abalone,
Haliotis rufescens from Baja California, Mexico. J Shellfish Res 20:
875-881, 2001.

Cheng W, Hsiao IS, Chen JC. Effect of ammonia on the immune response of
Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to
Vibrio parahaemolyticus. Fish Shellfish Immunol 17: 193-202, 2004a.
Cheng W, Hsiao IS, Chen JC. Effect of nitrite on immune response of Taiwan
abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio
parahaemolyticus. Dis Aquat Organ 60: 157-164, 2004b.

Cheng W, Hsiao IS, Hsu CH, Chen JC. Change in water temperature on the
immune response of Taiwan abalone Haliotis diversicolor supertexta
and its susceptibility to Vibrio parahaemolyticus. Fish Shellfish
Immunol 17: 235-243, 2004c.

Cheng W, Juang FM, Chen JC. The immune response of Taiwan abalone
Haliotis diversicolor supertexta and its susceptibility to Vibrio
parahaemolyticus at different salinity levels. Fish Shellfish Immunol 16:
295-306, 2000.

Cheng W, Yeh SP, Wang CS, Chen JC. Osmotic and ionic changes in Taiwan
abalone Haliotis diversicolor supertexta at different salinity levels.
Aquaculture 203: 349-357, 2002.

Cochennec N, Le Roux F, Berthe F, Gerard A. Detection of Bonamia ostreae
based on small subunit ribosomal probe. J Invert Path 76: 26-32, 2000.

Day R, Culver C, Kuris A, Belcher A, Morse D. The parasite Terebrasabella
heteroncinata (Polychaeta) manipulates shell synthesis in Haliotis
rufescens. J Shellfish Res 19: 507-512, 2000.

Elston RA, Lockwood GS. Pathogenesis of vibriosis in cultured juvenile red
abalone, Haliotis rufescens Swainson. J Fish Dis 6: 111-128, 1983.
Friedman CS, Andree KB, Beauchamp KA, Moore JD, Robbins TT, Shields
JD, Hedrick RP. ‘Candidatus Xenohaliotis californiensis‘, a newly
described pathogen of abalone, Haliotis spp., along the west coast of
North America. Int J Syst Evol Microbiol 50: 847-855, 2000.

Friedman CS, Biggs W, Shields JD and Hedrick RP (2002) Transmission of
Withering Syndrome in black abalone, Haliotis cracherodii Leach. J
Shellfish Res 21: 817-824, 2002.

Friedman CS, Finley CA. Anthropogenic introduction of the etiological agent
of withering syndrome into northern California abalone populations via
conservation efforts. Can J Fish Aquat Sci 60: 1424-1431, 2003.

Friedman CS, Thomson M, Chun C, Haaker PL, Hedrick, RP. Withering
syndrome of the black abalone, Haliotis cracherodii (Leach): Water
temperature, food availability, and parasites as possible causes. J.
Shellfish Res 16: 403-411, 1997.

Friedman CS. Coccidiosis of California abalone, Haliotis spp. J Shellfish Res
10: 236, 1991.

Gardner GR, Harshbarger JC, Lake JL, Sawyer TK, Price KL, Stephenson MD,
Haaker PL, Togstad HA. Association of prokaryotes with symptomatic
appearance of withering syndrome in black abalone Haliotis cracherodii.
J Invertebr Pathol 66: 111-120, 1995.

Goggin CL, Lester RJG. Perkinsus, a protistan parasite of abalone in
Australia: a review. Marine Fish Res 46: 639-646, 1995.

Haaker PL, Parker DO, Togstad H, Richards VD, Davis GE, Friedman CS.
Mass mortality and withering syndrome in black abalone Haliotis
cracherodii, in California. In: Shepherd SA TMJ, and Guzmaˊn del Proˊo
SA (ed) Abalone of the world: biology, fisheries, and culture. Oxford:
Fishing News Books, 214-224, 1992.

Kismohandaka G, Roberts W, Hedrick RP, Friedman CS. Physiological
alterations of the black abalone, Haliotis cracherodii Leach, with
withering syndrome. (Abstract) J Shellfish Res 14: 269-270, 1995.

Lafferty KD, Kuris AM. Mass mortality of abalone, Haliotis cracherodii, on
the California Channel Islands: tests of epidemiological hypotheses. Mar
Ecol Progr Ser 96: 239-248, 1993.

Leonard, M, Handlinger, JH, Powell, MD. Spionid mudworm infestation of
farmed abalone(Haliotis spp.). Aquaculture 221: 85-96, 2003.

Melissa MS. Cultured Abalone (Haliotis spp.) Seafood Watch Seafood
Report, 2003.

Millemann RE. Echinocephalus pseudouncinatus n.sp., a nematode parasite of
the abalone. J Parasitol 37: 435-439, 1951.

Moor JD, Cherr GN, Friedman CS. Detection of 'Candidatus Xenohaliotis
californiensis' (Rickettsiales-like prokaryote) inclusions in tissue
squashes of abalone (Haliotis spp.) gastrointestinal epithelium using
a nucleic acid fluorochrome. Dis Aquat Organ 46: 147-152, 2001.
Moore JD, Robbins TT, Friedman CS. Withering syndrome in farmed red
abalone Haliotis rufescens: Thermal induction and association with a
gastrointestinal rickettsia-like prokaryote. J Aquat Anim Health 16:26-
34, 2000.

Nakatsugawa T, Nagai T, Hiya K, Nishizawa T, and Muroga K. A virus
isolated from juvenile Japanese black abalone Nordotis discus discus
affected with amyotrophia. Dis Aquat Organ 36: 159-161, 1999.

Nicolas JL, Basuyaux O, Mazurie J, Thebault A. Vibrio carchariae, a pathogen
of the abalone Haliotis tuberculata. Dis Aquat Organ 50:35-43, 2002.

Nollens HH, Schofield JC, Keogh JA, Probert PK. Evaluation of radiography,
ultrasonography and endoscopy for detection of shell lesions in live
abalone Haliotis iris (Mollusca: Gastropoda). Dis Aqua Organ 50:
145-152, 2002.

Tsai JW, Chou YH, Chen BC, Liang HM, Liao CM. Growth toxicity bioassays
of abalone Haliotis diversicolor supertexta exposed to waterborne zinc.
Bull Environ Contam Toxicol 72: 70-77, 2004.

VanBlaricom GR, Ruediger JL, Friedman CS, Woodard DD, Hedrick RP.
Discovery of withering syndrome among black abalone, Haliotis
cracherodii Leach 1814, populations at San Nicolas Island, California. J
Shellfish Res 12: 185-188, 1993.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38642-
dc.description.abstract台灣鮑 (Haliotis diversicolor supertexta) 俗稱九孔或珍鮑魚,除了實
用與用價值外。同時亦具外銷潛力與競爭力,並成為我國輸出養殖水產品
主力之一。自2001年起,宜蘭縣地區的九孔苗陸續發生大量脫落死亡現象,
造成業者莫大損失,至今從北蔓延到高雄縣及台東縣,而澎湖亦有相似病
例發生。而於2003年1月下旬,在台北縣貢寮地區九孔養殖場,包括潮間帶
養殖池和陸上養殖池,陸續都有死亡情形。類似之病情,曾發生在大陸在
1999到2000年冬春季節,福建省東山、漳浦養殖九孔鮑魚爆發的大量死亡。
九孔幼苗發生脫落現象不只發生在台灣,自1999年起在中國大陸沿海,南
至海南島也陸續發生種苗大量脫落現象。由此可見,病害的發生是全面
性,而且可能是某一因素所造成。本實驗室曾就九孔苗部分進行病原分離
和病理學檢查。在病理學檢查及電子顯微鏡的觀察下發現Rickettisa-like
organism。相較於同屬不同種的紅鮑及黑鮑魚感染Rickettisa-like
organism,會因為溫度及食物來源與Rickettisa-like organism多寡造成紅鮑
魚及黑鮑魚的衰弱及死亡。因此,目前的結果尚無法確定Rickettisa-like
organism是造成九孔幼苗大量死亡之原因。
zh_TW
dc.description.abstractThe species of abalone cultured in Taiwan is belong to genus Haliotis,
family Haliotidae, and locally known as “nine hole” and “pearl abalone”. It’s
not only used to be traditional herb, but also be one of the important exported
goods in Taiwan’s aquiculture industry. The die-off of nursery abalone was
first occurred in Yielan in 2001 and the farmers suffering severe econimic loss.
The situation of nursery abalone die-off has spreaded out to Kaohsiung,
Taitung and Penhu. High mortality of adult abalone occurred in north eastern
Taiwan in January 2003. The abalone fishery in Dongshan and Zhangpu in
Fujian province also had similar mortality of adult abalone during 1999 winter
to 2000 spring. This situation quickly spread out along the Mainland China
coast from Shangdong province to Hainan Island. In the study, Rickettisa-like
organism has been found in adult abalone using hitopathology exam and
transmissible electronmicroscopy. The Rickettisales-like organism, which
cause withering syndrome is found in black abalone (Haliotis cracherodii) and
red abalone (H. rufescens). There mortality of balck abalone maybe related to
rickettisales-like organism intensity, temperature, and food supply. Results in
the study are unable to clarify the relationship between rickettsiales-like
organism and nursery abalone die-off.
en
dc.description.provenanceMade available in DSpace on 2021-06-13T16:40:16Z (GMT). No. of bitstreams: 1
ntu-94-R92629010-1.pdf: 15906931 bytes, checksum: bd533a69547388de1d86f009c82d7107 (MD5)
Previous issue date: 2005
en
dc.description.tableofcontents目錄-------------------------------------------------------------------------------------------------- I
圖次-------------------------------------------------------------------------------------------------- IV
表次-------------------------------------------------------------------------------------------------- V
中文摘要------------------------------------------------------------------------------------------ VI
英文摘要------------------------------------------------------------------------------------------- VII
第一章 前言------------------------------------------------------------------------------------- 1
第二章 文獻探討----------------------------------------------------------------------------- 3
第一節 九孔簡介------------------------------------------------------------------- 3
1.1 九孔分類與分佈----------------------------------------------------------- 3
1.2 九孔型態與棲息環境--------------------------------------------------- 3
1.3 九孔繁殖與生活史-------------------------------------------------------- 4
1.3.1 受精卵分裂與初期胚體發育------------------------------- 6
1.3.2 擔輪幼體 ( Trocophore )------------------------------- 6
1.3.3 面盤幼體 ( Veliger )-------------------------------------- 7
1.3.4 匍匐幼體 (Creeping larva)------------------------------ 7
1.3.5 圍口殼幼體 ( Peristomal shell )---------------------- 8
1.3.6 上足外化幼體------------------------------------------------------ 8
1.3.7 稚孔---------------------------------------------------------------------- 8
1.3.8 幼孔---------------------------------------------------------------------- 9
1.3.9 成孔---------------------------------------------------------------------- 9
第二節 九孔幼苗脫落之情形------------------------------------------------ 9
2.1 幼苗脫落起源及病害情形-------------------------------------------- 10
2.2 已知九孔疾病--------------------------------------------------------------- 10
2.3 新興的鮑魚疾病----------------------------------------------------------- 12
第三章 材料與方法------------------------------------------------------------------------- 15
第一節 九孔採樣---------------------------------------------------------------- 15
1.1 樣本收集------------------------------------------------------------------- 15
第二節 組織病理學檢查---------------------------------------------------- 16
2.1 檢查方式------------------------------------------------------------------- 16
第三節 病毒學檢查------------------------------------------------------------ 18
3.1 病毒檢查方式------------------------------------------------------------ 18
第四節 細菌分離---------------------------------------------------------------- 19
4.1 細菌分離方式------------------------------------------------------------ 19
第五節 穿透式電子顯微鏡檢查----------------------------------------- 22
5.1 穿透式電子顯微鏡樣品製備及檢查------------------------- 22
5.2 穿透式電子顯微鏡負染色觀察--------------------------------- 26
第六節 利用聚合酶鏈反應(PCR)---------------------------------- 27
6.1 DNA核酸之萃取-------------------------------------------------------- 27
6.2 聚合酶鏈反應(polymerase chain reaction, PCR)-- 28
6.3 DNA洋菜膠片電泳分析------------------------------------------ 29
第四章 實驗結果----------------------------------------------------------------------------- 31
第一節 組織病理學檢查------------------------------------------------ 31
第二節 病毒學檢查-------------------------------------------------------- 31
第三節 細菌分離------------------------------------------------------------- 31
第四節 利用聚合酶鏈反應(PCR)------------------------------- 32
第五節 電子顯微鏡超顯微構造之觀察------------------------- 32
第五章 討論------------------------------------------------------------------------------------- 34
第一節 可能之病原-------------------------------------------------------- 34
第二節 Rickettsiales-like prokaryote之可能-------------------- 34
第三節 Vibrio屬細菌之可能性--------------------------------------- 35
第四節 總結-------------------------------------------------------------------- 36
第六章 參考文獻----------------------------------------------------------------------------- 38
dc.language.isozh-TW
dc.subjectRickettisa-like organismzh_TW
dc.subject九孔zh_TW
dc.subject幼苗zh_TW
dc.subject大量死亡zh_TW
dc.subjectsmall abalone (Haliotis diversicolor supertexta)en
dc.subjectdie-offen
dc.title九孔幼苗大量死亡原因之探討zh_TW
dc.titleEtiology Study of Nursery Abalone Die-Off in Taiwanen
dc.typeThesis
dc.date.schoolyear93-2
dc.description.degree碩士
dc.contributor.oralexamcommittee陳三多,徐久忠,林文華
dc.subject.keyword九孔,幼苗,大量死亡,Rickettisa-like organism,zh_TW
dc.subject.keywordsmall abalone (Haliotis diversicolor supertexta),die-off,en
dc.relation.page49
dc.rights.note有償授權
dc.date.accepted2005-07-04
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept獸醫學研究所zh_TW
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