請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38478
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻 | |
dc.contributor.author | Chih-Shen Chiu | en |
dc.contributor.author | 邱志陞 | zh_TW |
dc.date.accessioned | 2021-06-13T16:34:47Z | - |
dc.date.available | 2005-07-20 | |
dc.date.copyright | 2005-07-20 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-08 | |
dc.identifier.citation | 1. Shimazaki J, Shinozaki N, Tsubota K. Transplantation of amniotic membrane and limbal autograft for patients with recurrent pterygium associated with symblepharon. Br J Ophthalmol 1998; 82: 235–240
2. Lee SH, Tseng SCG. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol 1997; 123: 303–312 3. Ray Jui-Fang Tsai, Lien-Min Li, Jan-Kan Chen. Reconstruction of Damaged Corneas by transplantation of autologous limbal epithelail cells. The new England Journal of Medicine 2000, 343: 86-93 4. Yasemin A. Katircioglu, Koray Budak, Sibel Salvarli and Sunay Duman. Amniotic membrane transplantation to reconstruct the conjunctival surface in cases of chemical burn. Jpn J Ophthalmol 2003; 47: 519–522 5. Tseng SCG, Prabhasawat P, Lee SH. Amniotic membrane transplantation for conjunctival surface reconstruction. Am J Ophthalmol 1997; 124: 765– 774 6. Tsai RJF, Tseng SCG. Human allograft limbal transplantation for corneal surface reconstruction. Cornea 1994;13:389-400. 7. Tsubota K, Satake Y, Ohyama M, et al. Surgical reconstruction of the ocular surface in advanced ocular cicatricial pemphigoid and Stevens Johnson syndrome. Am J Ophthalmol 1996; 122: 38 –52 8. Honovar S, Bansal A, Sangwan V, Rao G. Amniotic membrane transplantation for ocular surface reconstruction in Stevens-Johnson syndrome. Ophthalmology 2000; 107: 975–979. 9. Yong-Woo Cho, Yong-Nam Cho, Sang-Hun Chung, Gyeol Yoo, Sohk-Won Ko. Water-soluble chitin as a wound healing accelator. Biomaterials 20, 2139-2145(1999) 10. Pamela J. VandeVord, Howard W.T Matthew, Stephen P.Desilva, rt al. Evaluation of the biocompatibility of a chitosan scaffold in mice. J Biomed Mater Res 59; 585-590(2002) 11. K.Y. Lee, I.C. Kwon, Y.H. Kim, W.H. Jo, S.Y. Jeong. Preparation of chitosan self-aggregates as a gene delivery system. Journal of Controlled release, 51, 213-220(1998) 12. Angela M. De Campos, Alejandro Sanchez, Maria J. Alonso. Chitosan nanoparticles: a new vehicle for the improvement of the delivery of drugs to the ocular surface. Application to cyclosporine A Int. J. Phar. 224(2001)159-168 13. Strathmann, H., “Membrane separation processes,” J. Membrane Sci. 9 (1981), 121-189 14. O. Gris, A. Lopez-Navidad, F. Caballero, Z. del Campo, and A. Adan. Amniotic membrane transplantation for ocular surface pathology: long-term results. Transplantation Proceedings, 35, 2031-2035(2003) 15. Gerrit Borchard. Chitosans for gene delivery. Advanced Drug Delivery Reviews, 52, 145-150(2001) 16. YI ZHANG, RASHID A. AKHTAR. Effect of Epidermal Growth Factor on Phosphatidylinositol 3-kinase Activity in Rabbit Corneal Epithelial Cells. Exp. Eye Res., 63, 265-275(1996) 17. Volonte, C., Ciotti, M.T., and Battistini, L., “Development of a method for messuring cell number: Application to CNS primary neuronal culture,” Cytometry, 17(1994), 274-276 18. Benjamin, H.S., Li, L., and Yoon, P., “Thymic peptide protects vascular endotheial cells from hydrogen peroxide-induced oxidant injury,” Life. Sci., 52(1993), 1787-1796 19. P.CALVO, C.REMUNAN-LOPEZ, J. L. VILA-JATO, AND M.J. ALONSO, “NOVEL Hydrophilic Chitosan-Polyethylene Oxide Nanoparticles as Protein Carriers”, Journal of Applied Polymer Science 63, 125-132(1997) 20. Min Huang, Zengshuan Ma, Eugene Khor, and Lee-Yong Lim, “Uptake of FITC-Chitosan Nanoparticles by A549 Cells”, Pharmaceutical Research 19, 1488-1494(2002) 21. Umar Mahmood, Ching-Hsuan Tung, Alexei Bogdanov, Ralph Weissleder, “Near-Infrared Optical Imaging of Protease Activity for Tumor Detection”, Radiology 213, 866-870(1999) 22. Toshinori Sato, Tsuyoshi Ishii, Yoshio Okahata, “In vitro gene delivery mediated by chitosan. Effect of PH, serum, and molecular mass of chitosanon the transfection efficiency”, biomaterials 22, 2075-2080(2001) 23. Karin Corsi, Fatiha Chellat, L’Hocine Yahia, Julio C. Fernandes, “Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles”, biomaterials 24, 1255-1264(2003) 24. M. Thanou, B.I. Florea, M. Geldof, H.E. Junginger, G. Borchard, “Quaternized chitosan oligomers as novel gene delivery vectors in epithelial cell lines”, biomaterials 23, 153-159(2002) 25. Yutaka Ishino, Yoichiro Sano, Takahiro Nakamura, Che J. Connon, Helen Rigby, Nigel J. Fullwood, Shigeru Kinoshita, “Amniotic Membrane as a Carrier for Cultivated Human Corneal Endothelial Cell Transplantation”, Investigative Ophthalmology & Visual Science 45, 800-806(2004) 26. Andrew George, William G. Pitt, “Comparison of corneal epithelial cellular gowth on synthetic cornea materials”, Biomaterials 23, 1369-1373(2002) 27. Deborah F. Sweeney, Ruo Zhong Xie, Margaret D. M. Evans, Antti Vannas, Simon D. Tout, Hans J. Griesser, Graham Johnson, Jack G. Steele, “A Comparison of Biological Coatings for the Promotion of Corneal Epithelialization of Synthetic Surface In Vivo”, Investigative Ophthalmology & Visual Science 44, 3301-3309(2003) 28. M. D. M. Evans, S. Taylor, B. A. Dalton, D. Lohmann, “Polymer design for corneal epithelial tissue adhesion: Pore density”, J Biomed Mater Res 64A, 357-364(2003) 29. John G. Steele, Graham Johnson, Hans J. Griesser, P. Anne Underwood, “Mechanism of initial attachment of corneal epithelail cells to polymericsurfaces”, Biomaterials 18, 1541-1551(1997) 30. Margaret D. M. Evans, John G. Steele, “Polymer surface chemistry and a novel attachment mechanism in corneal epithelial cells”, J Biomed Mater Res 40,621-630(1997) 31. Der-Yuan Wang, Yi-Jen Hsueh, Vivian C. Yang, Jan-Kan Chen, “Propagation and Phenotypic Preservation of Rabbit Limbal Epithelial Cells on Amniotic Membrane”, Investigative Ophthalmology & Visual Science 44,4698-4704(2003) 32. Sandra W. Ryeom, David Paul, Daniel A. Goodenough, “Truncation Mutants of the Tight Junction Protein ZO-1 Disrupt Corneal Epithelial Cell Morphology”, Molecular Biology of the Cell 11, 1687-1696(2000) 33. MAYUMI MOCHIZUKI, YUICHI KADOYA, YOKO WAKABAYASHI, KOZUE KATO, IKUKO OKAZAKI, MASANORI YAMADA, TAKU SATO, NOBUO SAKAIRI, NORIO NISHI, MOTOYOSHI NOMIZU, “Laminin-1 peptide-conjugated chitosan membranes as a novel approach for cell engineering”, The FASEB Journal 17, 875-877(2003) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38478 | - |
dc.description.abstract | 近幾年之內利用組織工程技術來進行角膜上皮細胞的重建與修補,是以羊膜為主要的治療工具,本研究主要是將角膜上皮細胞培養在幾丁聚醣薄膜上,期望相比於羊膜具治療的效能。我們進而將幾丁聚醣作為奈米粒子,並利用奈米粒子的特性去做些相關的研究。
我們利用了MTT、免疫細胞化學染色與器官培養來比較幾丁聚醣薄膜與羊膜培養角膜上皮細胞的差異,由結果可以發現角膜上皮細胞在幾丁聚醣薄膜上具有良好的活性,可以證明幾丁聚醣是ㄧ具有良好生物相容性的生醫材料。而在免疫細胞化學染色與器官培養的部份來看,我們可以發現幾丁聚醣薄膜與羊膜的結果具有ㄧ制性,這也可以證明幾丁聚醣薄膜具有生物方面的活性可讓角膜上皮細胞重建或修補。而在奈米粒子的部份,我們利用了不同的染劑去證實胞飲作用;並將幾丁聚醣奈米粒子當作基因轉殖的載體,去與微脂粒做比較;也做了不同大小奈米粒子下對角膜上皮細胞的MTT與LDH影響,發覺幾丁聚醣奈米粒子並不具有明顯的毒性且在130nm的奈米粒子影響下,角膜上皮細胞的數目有增加的趨勢。 | zh_TW |
dc.description.abstract | In recent years, amniotic membranes transplantation is the most important advancement of treatment modality to treat ocular surface diseases. However, there are a lot of flaws for amniotic membranes transplantation. For example, the ethic issue and contamination of amniotic membrane make us to seek a new material to replace the usage of amniotic membrane. The purpose of this project is to evaluate the possibility of replacement of amnitoic membrane with chitosan membrane, which is a biocompatible materials used in many fields of biomedical science. We cultivated bovine corneal epithelial cells on chitosan membranes and evaluated their phenotypes.
MTT assay, indirect immunocytochemical staining and organ culture were used to compare the differences and evaluate the efficacy of treatment of corneal epithelial defects between amniotic membranes and chitosan membranes. According to the MTT assay, we found that the chitosan membrane was a good biomaterial for cultured corneal epithelial cells. In addition, the results of indirect immunocytochemical staining and organ culture, we found that chitosan membrane was compatible and as good as the use of amniotic membrane. Our results indicated that chitosan membrane is a good material for corneal tissue regeneration and repair. For nanoparticle studies, we used different dyes to study endocytosis of chitosan nanoparticles in cultured cells. We found that chitosan nanoparticles could transfect cultured cells with a less effeciency than the liposome. From the results of MTT and LDH assays, we found that chitosan nanoparticles were low toxic and 130 nm of chitosan nanoparticles were helpful to increase cell numbers. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T16:34:47Z (GMT). No. of bitstreams: 1 ntu-94-R92548031-1.pdf: 2861005 bytes, checksum: 9c4dadc02f4e4d2dc58bf9fd296a0422 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄............................................................................................................I
中文摘要..................................................................................................IV 英文摘要...................................................................................................V 圖表目錄.................................................................................................VII 第一章 背景..............................................................................................1 第二章 文獻回顧......................................................................................6 2-1高分子薄膜...................................................................................6 2-2 羊膜..............................................................................................7 2-3角膜上皮細胞的簡介...................................................................8 2-4幾丁聚醣奈米粒子…...................................................................8 第三章 材料與方法................................................................................10 3-1 實驗材料....................................................................................10 3-2 實驗儀器....................................................................................12 3-3 試劑配製....................................................................................16 3-4幾丁聚醣薄膜的製備.................................................................17 3-5分離純化角膜上皮細胞.............................................................18 3-6角膜上皮細胞的培養.................................................................19 3-6-1薄膜的前處理...................................................................19 3-6-2細胞的培養.......................................................................20 3-7細胞於材料上生長與死亡之評估.............................................20 3-7-1 MTT測試(評估細胞生長的情形)..................................20 3-7-2 LDH測試(評估細胞死亡的情形)..................................21 3-8間接免疫細胞化學染色.............................................................22 3-9器官培養.....................................................................................23 3-10奈米粒子粒徑大小與表面電量的測定...................................24 3-10-1製作幾丁聚醣奈米粒子.................................................24 3-10-2測定大小與電位.............................................................25 3-11奈米粒子的改質........................................................................26 3-11-1接枝螢光染劑.................................................................26 3-11-2接枝近紅外光染劑....….................................................27 3-11-3與脫氧核糖核酸結合...........................................……..27 3-12大小不同奈米粒子的MTT與LDH測試.................................29 第四章 結果與討論................................................................................30 4-1角膜上皮細胞在不同基材上培養情形:...................................30 4-2角膜上皮細胞對於不同大小奈米粒的活性與毒性測試.........31 4-3奈米粒子大小與濃度的關係.....................................................33 4-4角膜上皮細胞吞噬奈米粒子的情形.........................................34 4-5微脂粒與奈米粒子當作載體之轉殖.........................................36 4-6大小不同奈米粒子的MTT與LDH測試.................................37 4-7器官培養的情形………………………….................................40 第五章 結論............................................................................................43 參考文獻..................................................................................................44 附圖..........................................................................................................48 | |
dc.language.iso | zh-TW | |
dc.title | 開發幾丁聚醣薄膜及其奈米粒子進行培養角膜上皮細胞之研究 | zh_TW |
dc.title | Studies on the effect of chitosan membranes and chitosan nanoparticles on the behavior of corneal epithelial cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 王ㄧ中 | |
dc.contributor.oralexamcommittee | 王盈錦,胡芳蓉,蔡瑞芳 | |
dc.subject.keyword | 幾丁聚醣薄膜,幾丁聚醣奈米粒子, | zh_TW |
dc.subject.keyword | chitosan membranes,chitosan nanoparticles, | en |
dc.relation.page | 66 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-08 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-94-1.pdf 目前未授權公開取用 | 2.79 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。