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  1. NTU Theses and Dissertations Repository
  2. 生物資源暨農學院
  3. 獸醫專業學院
  4. 獸醫學系
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38375
完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor劉振軒
dc.contributor.authorYi-Cheng Changen
dc.contributor.author張益誠zh_TW
dc.date.accessioned2021-06-13T16:31:47Z-
dc.date.available2005-07-14
dc.date.copyright2005-07-14
dc.date.issued2005
dc.date.submitted2005-07-11
dc.identifier.citation1. 劉振軒、何逸僊、張文發、祝志平、王綉真。組織病理染色技術與圖譜。臺灣養豬科學研究所。1996。
2. 闕玲玲、鄭穹翔、潘銘正、龐飛、劉振軒、梁鍾鼎、李淑慧、張國慧。
動物疫病診斷鑑定技術研習會專輯。國立台灣大學獸醫學系。2003。
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7. Cooper JE, Gschmeissner S, Bone RD. Herpes-like virus particles in necrotic stomatitis of tortoises. Vet Rec 123: 544, 1988.
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22. Herbst LH, Jacobson ER, Klein PA, Balazs GH, Moretti R, Brown T, Sundberg JP. Comparative pathology and pathogenesis of spontaneous and experimentally induced fibropapillomas of green turtles (Chelonia mydas). Vet Pathol 36: 551-564, 1999.
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27. Jacobson ER, Clubb S, Gaskin JM, Gardiner C. Herpesvirus-like infection in Argentine tortoises. J Am Vet Med Assoc 187: 1227-1229, 1985.
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29. Jacobson ER, Gaskin JM, Wahlquist H. Herpesvirus-like infection in map turtles. J Am Vet Med Assoc 181: 1322-1324, 1982.
30. Kojimoto A, Uchida K, Horii Y, Okumura S, Yamaguch R, Tateyama S. Amebiasis in four ball pythons, Python reginus. J Vet Med Sci 63: 1365-1368, 2001.
31. Lange H, Herbst W, Wiechert JM, Schliesser T. [Electron microscopic detection of herpesviruses in a mass death of Greek tortoises (Testudo hermanni) and four-toed turtles (Agrionemys horsfieldii)]. Tierarztl Prax 17: 319-321, 1989.
32. Lu Y, Nerurkar VR, Aguirre AA, Work TM, Balazs GH, Yanagihara R. Establishment and characterization of 13 cell lines from a green turtle (Chelonia mydas) with fibropapillomas. In Vitro Cell Dev Biol Anim 35: 389-393, 1999.
33. Lu Y, Wang Y, Yu Q, Aguirre AA, Balazs GH, Nerurkar VR, Yanagihara R. Detection of herpesviral sequences in tissues of green turtles with fibropapilloma by polymerase chain reaction. Arch Virol 145: 1885-1893, 2000.
34. Lu YA, Wang Y, Aguirre AA, Zhao ZS, Liu CY, Nerurkar VR, Yanagihara R. RT-PCR detection of the expression of the polymerase gene of a novel reptilian herpesvirus in tumor tissues of green turtles with fibropapilloma. Arch Virol 148: 1155-1163, 2003.
35. Marschang RE, Becher P, Posthaus H, Wild P, Thiel HJ, Muller-Doblies U, Kalet EF, Bacciarini LN. Isolation and characterization of an iridovirus from Hermann's tortoises (Testudo hermanni). Arch Virol 144: 1909-1922, 1999.
36. Marschang RE, Gravendyck M, Kaleta EF. Herpesviruses in tortoises: investigations into virus isolation and the treatment of viral stomatitis in Testudo hermanni and T. graeca. Zentralbl Veterinarmed B 44: 385-394, 1997.
37. Marschang RE, Milde K, Bellavista M. Virus isolation and vaccination of Mediterranean tortoises against a chelonid herpesvirus in a chronically infected population in Italy. Dtsch Tierarztl Wochenschr 108: 376-379, 2001.
38. McLaughlin GS, Jacobson ER, Brown DR, McKenna CE, Schumacher IM, Adams HP, Brown MB, Klein PA. Pathology of upper respiratory tract disease of gopher tortoises in Florida. J Wildl Dis 36: 272-283, 2000.
39. Murakami M, Matsuba C, Une Y, Nomura Y, Fujitani H. Development of species-specific PCR techniques for the detection of tortoise herpesvirus. J Vet Diagn Invest 13: 513-516, 2001.
40. Muro J, Ramis A, Pastor J, Velarde R, Tarres J, Lavin S. Chronic rhinitis associated with herpesviral infection in captive spur-thighed tortoises from Spain. J Wildl Dis 34: 487-495, 1998.
41. Murphy FA, Gibbs EPJ, Horzinek MC, Studdert MJ. Veterinary Virology, 3rd ed, 1999.
42. Nigro O, Alonso Aguirre A, Lu Y. Nucleotide sequence of an ICP18.5 assembly protein (UL28) gene of green turtle herpesvirus pathogenically associated with green turtle fibropapilloma. J Virol Methods 120: 107-112, 2004.
43. Nigro O, Yu G, Aguirre AA, Lu Y. Sequencing and characterization of the full-length gene encoding the single-stranded DNA binding protein of a novel Chelonian herpesvirus. Arch Virol 149: 337-347, 2003.
44. Origgi FC, Klein PA, Mathes K, Blahak S, Marschang RE, Tucker SJ, Jacobson ER. Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]). J Clin Microbiol 39: 3156-3163, 2001.
45. Origgi FC, Klein PA, Tucker SJ, Jacobson ER. Application of immunoperoxidase-based techniques to detect herpesvirus infection in tortoises. J Vet Diagn Invest 15: 133-140, 2003.
46. Origgi FC, Romero CH, Bloom DC, Klein PA, Gaskin JM, Tucker SJ, Jacobson ER. Experimental transmission of a herpesvirus in Greek tortoises (Testudo graeca). Vet Pathol 41: 50-61, 2004.
47. Ozaki K, Matsuo K, Tanaka O, Narama I. Amoebosis in the flat-shelled spider tortoise (Acinixys planicauda). J Comp Pathol 123: 299-301, 2000.
48. Pettan-Brewer KCB, Drew ML, Ramsay E, Mohr FC, Lowenstine LJ. Herpesvirus particles associated with oral and respiratory lesions in a California desert tortoise (Gopherus agassizii). J Wildl Dis 32: 521-526, 1996.
49. Quackenbush SL, Casey RN, Murcek RJ, Paul TA, Work TM, Limpus CJ, Chaves A, duToit L, Perez JV, Aguirre AA, Spraker TR, Horrocks JA, Vermeer LA, Balazs GH, Casey JW. Quantitative analysis of herpesvirus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR. Virology 287: 105-111, 2001.
50. Quackenbush SL, Work TM, Balazs GH, Casey RN, Rovnak J, Chaves A, duToit L, Baines JD, Parrish CR, Bowser PR, Casey JW. Three closely related herpesviruses are associated with fibropapillomatosis in marine turtles. Virology 246: 392-399, 1998.
51. Rebell G, Rywlin A, Haines H. A herpesvirus-type agent associated with skin lesions of green sea turtles in aquaculture. Am J Vet Res 36: 1221-1224, 1975.
52. Schumacher IM, Brown MB, Jacobson ER, Collins BR, Klein PA. Detection of antibodies to a pathogenic mycoplasma in desert tortoises (Gopherus agassizii) with upper respiratory tract disease. J Clin Microbiol 31: 1454-1460, 1993.
53. Soares JF, Chalker VJ, Erles K, Holtby S, Waters M, McArthur S. Prevalence of Mycoplasma agassizii and Chelonian herpesvirus in captive tortoises (Testudo sp.) in the United Kingdom. J Zoo Wildl Med 35: 25-33, 2004.
54. Stedman NL, Munday JS, Esbeck R, Visvesvara GS. Gastric amebiasis due to Entamoeba histolytica in a Dama wallaby (Macropus eugenii). Vet Pathol 40: 340-342, 2003.
55. Teifke JP, Lohr CV, Marschang RE, Osterrieder N, Posthaus H. Detection of chelonid herpesvirus DNA by nonradioactive in situ hybridization in tissues from tortoises suffering from stomatitis-rhinitis complex in Europe and North America. Vet Pathol 37: 377-385, 2000.
56. Telford EA, Watson MS, Perry J, Cullinane AA, Davison AJ. The DNA sequence of equine herpesvirus-4. J Gen Virol 79: 1197-1203, 1998.
57. Une Y, Murakami M, Uemura K, Fujitani H, Ishibashi T, Nomura Y. Polymerase chain reaction (PCR) for the detection of herpesvirus in tortoises. J Vet Med Sci 62: 905-907, 2000.
58. Une Y, Uemura K, Nakano Y, Kamiie J, Ishibashi T, Nomura Y. Herpesvirus infection in tortoises (Malacochersus tornieri and Testudo horsfiedii). Vet Pathol 36: 624-627, 1999.
59. VanDevanter DR, Warrener P, Bennett L, Schultz ER, Coulter S, Garber RL, Rose TM. Detection and analysis of diverse herpesviral species by consensus primer PCR. J Clin Microbiol 34: 1666-1671, 1996.
60. Wellehan JF, Johnson AJ, Latimer KS, Whiteside DP, Crawshaw GJ, Detrisac CJ, Terrell SP, Heard DJ, Childress A, Jacobson ER. Varanid herpesvirus 1: a novel herpesvirus associated with proliferative stomatitis in green tree monitors (Varanus prasinus). Vet Microbiol 105: 83-92, 2005.
61. Wellehan JF, Nichols DK, Li LL, Kapur V. Three novel herpesviruses associated with stomatitis in Sudan plated lizards (Gerrhosaurus major) and a black-lined plated lizard (Gerrhosaurus nigrolineatus). J Zoo Wildl Med 35: 50-54, 2004.
62. Yu Q, Hu N, Lu Y, Nerurkar VR, Yanagihara R. Rapid acquisition of entire DNA polymerase gene of a novel herpesvirus from green turtle fibropapilloma by a genomic walking technique. J Virol Methods 91: 183-195, 2001.
63. Yu Q, Lu Y, Nerurkar VR, Yanagihara R. Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma Brief report. Arch Virol 145: 2669-2676, 2000.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38375-
dc.description.abstract自1999年1月至2005年4月,收集因消化道感染死亡之圈飼陸龜共56隻及採集144隻圈飼陸龜之口腔拭子進行病理學檢查、穿透式電子顯微鏡檢查及巢式聚合酶鏈反應檢測。罹病陸龜常見之臨床症狀為黃白色分泌物分別由口腔與鼻腔流出、食慾不振、沈鬱及眼瞼腫脹。由肉眼病理學與組織病理學檢查,有18隻(18/56, 32.1﹪)呈現壞死性口炎、胃炎與腸炎,其中有3隻(3/18, 16.6﹪),於胃(1隻)與小腸(2隻)之壞死病灶區伴隨出現嗜酸性核內包涵體,感染之品系分別為1隻四爪陸龜、1隻紅腿象龜與1隻蘇卡達象龜。以穿透式電子顯微鏡觀察口腔與胃組織中之包涵體,可見典型疱疹病毒之病毒顆粒分佈在黏膜上皮細胞之細胞核與細胞質內,病毒顆粒之直徑大約100 nm、具有封套、核蛋白衣、與電子緻密小體。採集144隻陸龜之口腔拭子及罹病陸龜之口腔與胃組織,經由巢式聚合酶鏈反應檢測疱疹病毒核酸,有3個口腔拭子與1個胃組織呈現陽性反應,感染品系分別為2隻四爪陸龜、1隻輻射龜與1隻緬甸星龜。由病理學、穿透式電子顯微鏡與巢式聚合酶鏈反應,證實國內圈飼陸龜已有疱疹病毒感染病例而死亡。此為台灣地區首次報告陸龜疱疹病毒感染症。zh_TW
dc.description.abstractBetween January in 1999 and April in 2005, a total of 56 necropsies of tortoises with clinical signs of oral and nasal discharge, anorexia, lethargy, and palpebral edema and 144 oral swabs were collected. On gross and microscopic examination, 18 of 56 necropsies (32.1%) showed varying degrees of necrotic lesions distributed in the oral cavity and gastrointestinal tract. Three of 18 necropsies (16.6%) with digestive lesions showed marked necrotizing gastroenteritis associated with intralesional eosinophilic intranuclear inclusion bodies (INIB), in which 1 case in the stomach and 2 cases in the small intestine were observed in Geochelone carbonaria, Geochelone sulcata, and Testudo horsfieldi. Electron microscopic examination of INIB in the oral tissue and stomach showed numerous herpesviral particles about 100 nm in diameter in the nuclei and cytoplasm of mucosal epithelium. The viral particles contained envelope, nucleocapsids, and an electron-dense body. Nested polymerase chain reaction analysis using a herpesvirus consensus primer method confirmed the presence of herpesvirus DNA in three oral swabs and stomach. Based on the clinical signs, and the results of pathological examination, electron microscopy and nest polymerase chain reaction, herpesvirus infection was diagnosed. This is the first report to verify herpesvirus infection of tortoises in Taiwan, which induced digestive lesions and caused fatal outcome in some cases.en
dc.description.provenanceMade available in DSpace on 2021-06-13T16:31:47Z (GMT). No. of bitstreams: 1
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Previous issue date: 2005
en
dc.description.tableofcontents目錄------------------------------------------------------I
中文摘要----------------------------------------------- III
英文摘要------------------------------------------------ IV
表次與圖次---------------------------------------------- V
第一章 緒言--------------------------------------------1
第二章 文獻探討----------------------------------------- 4
第一節 陸龜疱疹病毒感染症之歷史背景----------------------4
第二節 陸龜疱疹病毒之宿主範圍----------------------------7
第三節 陸龜疱疹病毒之病毒特性----------------------------7
第四節 陸龜疱疹病毒感染症之傳播途徑與流行病學------------8
第五節 陸龜疱疹病毒感染症之診斷-------------------------10
2.5.1 臨床症狀------------------------------------------10
2.5.2 血液學與血清生化學--------------------------------10
2.5.3 病理學--------------------------------------------11
2.5.4 穿透式電子顯微鏡----------------------------------12
2.5.5 聚合酶鏈反應--------------------------------------12
2.5.6 酵素結合免疫吸附法--------------------------------14
2.5.7 血清中和試驗--------------------------------------15
2.5.8 免疫組織化學染色----------------------------------16
2.5.9 病毒分離------------------------------------------17
2.5.10 原位雜合反應--------------------------------------18
第六節 陸龜疱疹病毒感染症之預防與控制-------------------19
第三章 材料與方法 --------------------------------------20
第一節 實驗設計及流程圖-------------------------------- 20
第二節 材料來源-----------------------------------------21
第三節 形態病理學檢查---------------------------------- 23
3.3.1 肉眼病理學檢查------------------------------------23
3.3.2 組織病理學檢查------------------------------------23
3.3.3 穿透式電子顯微鏡檢查----------------------------- 27
3.3.4 Periodic Acid Schiff(PAS)法染多醣類------------ 32
第四節 利用巢式聚合酶鏈反應檢測陸龜疱疹病毒之感染------ 34
3.4.1引子(primer)之選定------------------------------- 35
3.4.2病毒核酸之萃取--------------------------------------35
3.4.3巢式聚合酶鏈反應------------------------------------38
3.4.4聚合酶鏈反應之電泳分析----------------------------- 40
第四章 結果-------------------------------------------- 41
4.1.1 臨床症狀----------------------------------------- 41
4.1.2 病理學------------------------------------------- 41
4.1.3 穿透式電子顯微鏡檢查之結果----------------------- 43
4.1.4 巢式聚合酶鏈反應檢測之結果----------------------- 43
第五章 討論----------------------------------------------56
第六章 未來展望----------------------------------------- 60
第七章 參考文獻----------------------------------------- 61
dc.language.isozh-TW
dc.title應用形態病理學、穿透式電子顯微鏡及聚合酶鏈反應診斷陸龜疱疹病毒感染症zh_TW
dc.titleThe Diagnosis of Herpesvirus Infection in Tortoises by Pathology, Transmission Electron Microscopy and Polymerase Chain Reactionen
dc.typeThesis
dc.date.schoolyear93-2
dc.description.degree碩士
dc.contributor.oralexamcommittee蕭正祥,張本恆,張清棟,李進成
dc.subject.keyword陸龜疱,疹病毒感染症,巢式聚合&#37238,鏈反應,陸龜,zh_TW
dc.subject.keywordHerpesvirus infection,nested polymerase chain reaction,tortoises,en
dc.relation.page73
dc.rights.note有償授權
dc.date.accepted2005-07-11
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept獸醫學研究所zh_TW
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