請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/3830完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孫珍理(Chen-li Sun) | |
| dc.contributor.author | Hung-Yi Lin | en |
| dc.contributor.author | 林泓毅 | zh_TW |
| dc.date.accessioned | 2021-05-13T08:37:16Z | - |
| dc.date.available | 2019-08-24 | |
| dc.date.available | 2021-05-13T08:37:16Z | - |
| dc.date.copyright | 2016-08-24 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-01 | |
| dc.identifier.citation | [1] T. M. Keenan, C. W. Frevert, A. Wu, V. Wong, and A. Folch, 'A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber,' Lab on a Chip, vol. 10, no. 1, pp. 116-122, 2010. (doi: 10.1039/b913494h)
[2] N. L. Jeon, S. K. W. Dertinger, D. T. Chiu, I. S. Choi, A. D. Stroock, and G. M. Whitesides, 'Generation of solution and surface gradients using microfluidic systems,' Langmuir, vol. 16, no. 22, pp. 8311-8316, 2000. (doi: 10.1021/la000600b) [3] D. Irimia, S.-Y. Liu, W. G. Tharp, A. Samadani, M. Tonera, and M. C. Poznansky, 'Microfluidic system for measuring neutrophil migratory responses to fast switches of chemical gradients,' Lab on a Chip, vol. 6, no. 2, pp. 191-198, 2005. (doi: 10.1039/b511877h) [4] S.-Y. Cheng, S. Heilman, M. Wasserman, S. Archer, M. L. Shulerac, and M. Wu, 'A hydrogel-based microfluidic device for the studies of directed cell migration,' Lab on a Chip, vol. 7, no. 6, pp. 763-769, 2007. (doi: 10.1039/b618463d) [5] M. Kim, M. Jia, Y. Kim, and T. Kim, 'Rapid and accurate generation of various concentration gradients using polydimethylsiloxane-sealed hydrogel device,' Microfluidics and Nanofluidics, vol. 16, no. 4, pp. 645-654, 2013. (doi: 10.1007/s10404-013-1265-y) [6] Y. Ge, Q. An, Y. Gao, Y. Chen, and D. Li, 'A microfluidic device for generation of chemical gradients,' Microsystem Technologies, vol. 21, no. 8, 2014. (doi: 10.1007/s00542-014-2287-4) [7] J. J. VanDersarl, A. M. Xu, and N. A. Melosh, 'Rapid spatial and temporal controlled signal delivery over large cell culture areas,' Lab on a Chip, vol. 11, no. 18, pp. 3057-3063, 2011. (doi: 10.1039/c1lc20311h) [8] P.-Y. Chang, K.-W. Chang, C.-W. Lin, P.-C. Shih, C.-H. Liu, C.-J. Li, C.-H. Tien, H.-Y. Huang, W.-S. Hsu, S.-K. Fan, and D.-J. Yao, 'Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells,' presented at the Nano/Molecular Medicine and Engineering, 2012 IEEE 6th International Conference on, USA, IEEE, 4-7 November, 2012. [9] D. Huh, H. J. Kim, J. P. Fraser, D. E. Shea, M. Khan, A. Bahinski, G. A. Hamilton, and D. E. Ingber, 'Microfabrication of human organs-on-chips,' Nature Protocols, vol. 8, no. 11, pp. 2135-2157, 2013. (doi: 10.1038/nprot.2013.137) [10] H. Wei, B.-h. Chueh, H. Wu, E. W. Hall, C.-w. Li, R. Schirhagl, J.-M. Lin, and R. N. Zare, 'Particle sorting using a porous membrane in a microfluidic device,' Lab on a Chip, vol. 11, no. 2, pp. 238-245, 2010. (doi: 10.1039/c0lc00121j) [11] J. M. Karlsson, T. Haraldsson, C. F. Carlborg, J. Hansson, A. Russom, and W. v. derWijngaart, 'Fabrication and transfer of fragile 3D PDMS microstructures,' Journal of Micromechanics and Microengineering, vol. 22, no. 8, 2012. (doi: 10.1088/0960-1317/22/8/085009) [12] X. Fan, C. Jia, J. Yang, G. Li, H. Mao, Q. Jin, and J. Zhao, 'A microfluidic chip integrated with a high-density PDMS-based microfiltration membrane for rapid isolation and detection of circulating tumor cells,' Biosensors and Bioelectronics, vol. 71, pp. 380-386, 2015. (doi: 10.1016/j.bios.2015.04.080) [13] R. D. Sjoblad, I. Chet, and R. Mitchell, 'Quantitative assay for algal chemotaxis,' Applied and Environmental Microbiology, vol. 36, no. 6, pp. 847-850, 1978. (doi: 0099-2240/78/0036-0847$02.00/0) [14] H. Jeon, Y. Lee, S. Jin, S. Koo, C.-S. Lee, and J. Y. Yoo, 'Quantitative analysis of single bacterial chemotaxis using a linear concentration gradient microchannel,' Biomedical Microdevices, vol. 11, no. 5, pp. 1135-1143, 2009. (doi: 10.1007/s10544-009-9330-8) [15] G.-X. Zheng, Y.-J. Li, L.-L. Qi, X.-M. Liu, H. Wang, S.-P. Yu, and Y.-H. Wang, 'Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment,' Marine Pollution Bulletin, vol. 84, no. 1, pp. 147-154, 2014. (doi: 10.1016/j.marpolbul.2014.05.019) [16] C. Meinhart, S. Wereley, and M. Gray, 'Volume illumination for two-dimensional particle image velocimetry,' Measurement Science and Technology, vol. 11, no. 6, p. 809, 2000. (doi: 10.1088/0957-0233/11/6/326) [17] MATLAB, version R2015a (8.5.0 197613): MathWorks, 2015. [18] L. J. Chung, 'Computational Fluid Dynamics,' 1st ed: Cambridge University Press, 2002. [19] N.-T. Nguyen, Micromixers: Norwich, NY: William Andrew, 2008. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/3830 | - |
| dc.description.abstract | 本研究目的為針對探討濃度對浮游生物影響的研究，設計一能操控濃度梯度之微流元件。所設計的微流元件為一三層結構，皆以PDMS構成，其中包含上層的濃度梯度供給流道、中層的多孔薄膜與下層的微孔穴流道。本三層構造之目的為分離上層流道與下層流道內之流場，並藉由中層之多孔薄膜層在孔穴中產生濃度梯度。下層為一孔穴觀察區，連接截面為矩形之主流道，上層之濃度梯度供給流道為對稱的雙流道設計，各別通入高濃度與低濃度之流體，以在下層之孔穴內產生濃度梯度，藉由設計多孔薄膜之孔隙數量與孔徑大小，改變濃度梯度的分布型態。
實驗結果顯示，微孔穴流道之流場最容易影響微孔穴內之濃度場，微孔穴流道之Reynolds number增加時，孔穴內平均濃度會明顯降低，但分布差異不大。此外，濃度梯度供給流道的對齊方向可以明顯改變濃度梯度的分布，若與微孔穴流道的主流道平行，濃度在y軸方向的變化較為劇烈，靠近 +y方向之濃度較高；若與微孔穴流道的主流道垂直，濃度在x軸方向的變化較為劇烈，靠近 -x方向之濃度較高。因此若要改變微孔穴內之濃度梯度場，改變濃度梯度供給流道之對齊方向的效果是最好的。此外，在濃度梯度供給流道內的流向為反向流時，孔穴內濃度場較不易受濃度梯度供給流道之Reynolds number影響。薄膜的孔隙過多時會造成孔隙率過大，使微孔穴流道之流場影響濃度梯度供給流道，染劑不易通過孔隙，因此不宜選用孔隙率過大之薄膜。然而，使用較大孔隙之薄膜時，產生之濃度梯度場較為集中，峰值可達到1500 m-1。本研究所得之結果可協助我們得知，藉由改變濃度梯度供給流道與微孔穴流道之流場，可改變孔穴內之濃度場變化，且孔隙率較低之薄膜可降低兩層流道間流場之影響，以利未來進行浮游生物同時受流場與濃度場改變之趨化性研究。 | zh_TW |
| dc.description.abstract | This study focuses on designing a microfluidic device to generate nutrient gradient for the study of plankton in chemotaxis in the future. The PDMS microfluidic platform is consisted of three layers: the gradient channel, the porous membrane, and the microcavity channel. The 3 layer structure is aimed at generating concentration variation in the microcavity by reducing the influence from the flow field of the gradient channel. The micro cavity channel has a circular pocket connected to a straight channel. The gradient channel has two symmetrical channels, one for water and the other for the dye solution. By transport through the perforated membrane, concentration gradient is formed in the microcavity. The alignment of the gradient channel and the microcavity channel of the bottom layer can be changed to alter the concentration gradient is formed in the microcavity. In addition, we design membrane with different pore size and pitch to study their effect on the concentration distribution in the microcavity.
The result shows that varying the flow velocity of the microcavity channel is the easiest way to change the concentration field. When velocity increases, the average concentration in the microcavity decreases, without significant change of its distribution. In addition, The direction of the concentration gradient can be easily change by the orientation between the gradient and the microcavity channels. When the gradient channel is aligned parallelly to the straight channel of the microcavity layer, higher concentration is found in the bottom of the pocket. When the gradient channel is aligned perpendicularly to the straight channel of the microcavity layer, higher concentration is found near the left edge of the microcavity. For the counterflow configuration in the gradient channel, the concentration is not affected by the flow in the microcavity channel. When the membrane has more pores, porosity too large such that the flow in the microcavity channel may interfere the flow in the gradient channel, which makes the dye more difficult to pass through the pores. Therefore, membrane with large porosity is not recommended. Yet, lager pore size lead to higher concentration gradient in the cavity and the pick value can reach as higher as 1500 m-1. In this study, we successfully manipulate the concentration field in the microcavity by varying the flow field in the gradient channel and the microcavity channel. To reduce the effect of flow field on the concentration distribution, membrane with lower porosity is preferred. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-13T08:37:16Z (GMT). No. of bitstreams: 1 ntu-105-R03522303-1.pdf: 9479014 bytes, checksum: 8a9a5a13252b3625fa55375ea1639cf9 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iv 目錄 vi 符號索引 ix 表目錄 xii 圖目錄 xiii 第一章 導論 1 1.1 前言 1 1.2 文獻回顧 2 1.2.1 研究趨化性之微流元件設計 2 1.2.2 多孔薄膜與其製程設計 4 1.2.3 趨化性對細菌與浮游生物之影響 5 1.3 研究動機 6 第二章 元件設計、製程與實驗程序 7 2.1 微營養鹽濃度梯度元件設計 7 2.1.1 濃度梯度供給流道 7 2.1.2 微孔穴流道 8 2.1.3 多孔薄膜層 8 2.2 微濃度梯度元件製程 9 2.2.1 矽晶圓清洗 9 2.2.2 母模製作 10 2.2.3 PVA-PDMS Stack製程 12 2.2.4 PDMS流道製程 13 2.2.5 使用PDMS多孔薄膜之微營養鹽濃度梯度元件組裝製程 14 2.3 實驗架構 16 2.3.1 倒立式顯微鏡 17 2.3.2 工作流體 17 2.4 實驗量測程序 18 2.4.1 濃度量化校正實驗 18 2.4.2 濃度梯度場量化 20 2.4.3 孔隙率量測 21 2.4.4 擴散係數量測 23 2.5 不確定性分析 23 2.5.1 體積流率之相對不確定性 23 2.5.2 Reynolds number之相對不確定性 24 2.5.3 調配工作流體之重量分率的相對不確定性 25 2.5.4 調配工作流體之濃度的相對不確定性 26 2.5.5 正規化影像灰階值之相對不確定性 26 2.5.6 影像分析之濃度的相對不確定性 27 2.5.7 影像分析之濃度梯度的相對不確定性 28 2.5.8 孔隙率量測之相對不確定性 29 第三章 結果與討論 31 3.1 孔穴內濃度場分析 31 3.1.1 微孔穴流道的Reynolds number之影響 32 3.1.2 濃度梯度供給流道之流向與流速之影響 33 3.1.3 多孔薄膜孔隙數量對孔穴內濃度之影響 35 3.1.4 多孔薄膜孔徑對孔穴內濃度之影響 35 3.2 孔穴內濃度梯度場分析 36 3.2.1 微孔穴流道的Reynolds number之影響 37 3.2.2 濃度梯度供給流道之流向與流速之影響 37 3.2.3 多孔薄膜孔隙數量對濃度梯度之影響 38 3.2.4 多孔薄膜孔徑對孔穴內濃度梯度之影響 39 第四章 結論與建議 40 4.1 結論 40 4.2 建議 41 參考文獻 43 附表 45 附圖 49 | |
| dc.language.iso | zh-TW | |
| dc.subject | 趨化性 | zh_TW |
| dc.subject | 微流元件 | zh_TW |
| dc.subject | 濃度梯度 | zh_TW |
| dc.subject | 多孔PDMS薄膜 | zh_TW |
| dc.subject | 聚乙烯醇薄膜 | zh_TW |
| dc.subject | 可調控之微生態環境 | zh_TW |
| dc.subject | microfluidic device | en |
| dc.subject | manipulative microhabitat | en |
| dc.subject | PVA membrane | en |
| dc.subject | porous PDMS membrane | en |
| dc.subject | concentration gradient | en |
| dc.subject | chemotaxis | en |
| dc.title | 微營養鹽濃度梯度元件之設計 | zh_TW |
| dc.title | Designing a Microfluidic Device with Nutrient Gradient | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉耀先(Yao-Hsien Liu),林怡均(Yi-Jiun Lin) | |
| dc.subject.keyword | 微流元件,濃度梯度,多孔PDMS薄膜,聚乙烯醇薄膜,可調控之微生態環境,趨化性, | zh_TW |
| dc.subject.keyword | microfluidic device,concentration gradient,porous PDMS membrane,PVA membrane,manipulative microhabitat,chemotaxis, | en |
| dc.relation.page | 186 | |
| dc.identifier.doi | 10.6342/NTU201601252 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-08-02 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
| 顯示於系所單位： | 機械工程學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-105-1.pdf | 9.26 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。