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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林?輝 | |
dc.contributor.author | Wei-Ren Wang | en |
dc.contributor.author | 王尉任 | zh_TW |
dc.date.accessioned | 2021-06-13T16:26:32Z | - |
dc.date.available | 2005-07-20 | |
dc.date.copyright | 2005-07-20 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-15 | |
dc.identifier.citation | [1] 行政院衛生署, “中華民國九十三年臺灣地區死因統計結果摘要”, 2005
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Yamamuro, “Mechanism properties of a new type of apatite-containing glass-ceramics for prosthetic application”, J. Mater. Sci., 20: 2001-2004, 1985 [27] F. H. Lin and M. H. Hon, “A study on the bioglass ceramics in the Na2O-CaO-SiO2-P2O5 system”, J. Mater. Sci., 23: 4295-4299, 1989 [28] L.L. Hench and H.A. Paschall, 'Histochemical responses at a biomaterials Interface,' J. Biomed. Mater. Res., 5: 49–54, 1974 [29] T. Kokubo and S. Ito, 'Ca, P-rich layer formed on high-strength bioactive glass ceramic A-W,' J. Biomed. Mater. Res. 24: 331–343, 1987 [30] 王盈錦,林峰輝,”生醫陶瓷材料”, 生物醫學材料, 合記圖書出版社, 278-279, 2002 [31] M. Shinkai, M. Yanase, H. Honda, T. Wakabayashi, J. Yoshida and T. Kobayashi, “Intracellular hyperthermia for cancer using magnetic cationic liposomes: in vitro study”, Jpn. J. Cancer Res., 87: 1179-1183, 1996 [32] M. Shinkai, M. Yanase, H. Honda, T. Wakabayashi, J. Yoshida and T. Kobayashi, “Intracellular hyperthermia for cancer using magnetic cationic liposomes: in vivo study”, Jpn. 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Callister, “Magnetic properties”, Materials Science and Engineering: An Introduction, 5th ed., John Wiley & Sons, 688-690, 1999 [39]http://www.ndt-ed.org/EducationResources/CommunityCollege/MagParticle/ Physics/HysteresisLoop.htm [40] J. F. Shackelford, “Magnetic materials”, Introduction to Materials Science for Engineerings, 5th ed., 686-687, Prentice Hall International, 2001 [41] 陳繼仁, “磁性陶瓷”, 陶瓷技術手冊上, 570, 中華民國產業科技發展協進會, 1994 [42] B. E. Warren, Kristallogr. Mineralog. Petrogr., 86: 349, 1933 [43] V. M. Goldschmidt, “Geochemische Verteilungsgesetze der Elemente, Skrifter Norske Videnskaps Akad”, I. Math. Naturwiss. Kl. 8: 7-156, 1926 [44] W. H. Zachariasen, “The atomic arrangement in glass”, J. Am. Chem. Soc., 54: 3841, 1932 [45] W. D. Kingery, H. K. Bowen and D. R. Uhlmann, Introduction to Ceramics, 98, Wiley and Sons, 1976 [46] A. Dietzel, Z. Elektrochem., 48: 9-23, 1942 [47] W. Vogel, “Chemistry of glass”, Am. Ceram. Soc., 38, 1985 [48] C. J. Brinker, G. W. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38111 | - |
dc.description.abstract | 惡性腫瘤(癌症)是一種相當常見的疾病,尤其在我們所存在的環境當中,隨處都有各種不同的致癌因子,若不能夠有效的預防,則定期的健康檢查可以幫助早期發現並治療以避免其擴大的可能。在癌症治療方面,已有相當長時間的發展,最普遍的如外科手術、放射線治療及化學藥物等,皆為最常使用且有效的方式;除此之外,隨著生物資訊與醫療儀器在近幾年有突破性的發展,也有一些新的方法可以治療癌症,例如免疫療法與溫熱(高體溫)療法等,都是目前針對癌症治療最熱門的研究題目。
本研究是以溫熱療法為主題,利用本實驗室所開發的可分解性生醫玻璃作為基本材料,以溶膠-凝膠(sol-gel)技術合成出具有磁性的粉末,再以磁滯曲線分析其磁性質。當我們提供交流電感應磁場時,材料因磁滯損失而產生熱能,使材料的溫度上升,經測試後可知其有相當好的生物相容性,若將它表面改質後再經靜脈注射入人體內,則可以達到殺死癌細胞的目的,藉此提供溫熱療法更有效且無害人體的新選擇。 | zh_TW |
dc.description.abstract | The malignant tumor (cancer) is a common disease, because there are many carcinogens existing in the surrounding environment. Many therapeutic methods have been developed for cancer treatment which included surgery, radiotherapy, and chemical medicine. But none among those was matched to clinical satisfaction. In recent years, due to well-developed medical instruments and biotechnologies, some new methods have been developed for cancer treatment. For example, immunotherapy, hyperthermia, etc., all turned out to be the most popular research topics for cancer therapy.
This research is aimed to develop a biodegradable bioglass on cancer hyperthermia. We choose bioglass as the basic material and in cooperate the magnetic powder into the bioglass by sol-gel method. The hysteresis loop will be used to analyze the magnetic and thermal-induced property. After exposure to an alternating magnetic field, we can find out that the temperature of the material increased due to hysteresis loss. The results of cytotoxicity tests showed no harmful to the cell adhesion, proliferation and differentiation. If the bioglass with surface modification injected into human body in the future, it can be a new choice for hyperthermia to kill tumor cell more efficiently and without doing harm to the human body. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T16:26:32Z (GMT). No. of bitstreams: 1 ntu-94-R92548033-1.pdf: 2357212 bytes, checksum: 2d01334bc2ef466d6a8c1551719ec468 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 中文摘要-------------------------------------------------------------------------------------------I
英文摘要------------------------------------------------------------------------------------------II 目錄-----------------------------------------------------------------------------------------------III 圖目錄--------------------------------------------------------------------------------------------VI 表目錄--------------------------------------------------------------------------------------------IX 符號索引------------------------------------------------------------------------------------------X 第一章 簡介 1-1 前言----------------------------------------------------------------------------------------1 1-2 認識癌症----------------------------------------------------------------------------------2 1-3 癌症治療 1-3-1 外科手術----------------------------------------------------------------------------3 1-3-2 放射線療法-------------------------------------------------------------------------4 1-3-3 化學藥物療法----------------------------------------------------------------------6 1-3-4 免疫療法----------------------------------------------------------------------------8 1-3-5 溫熱療法----------------------------------------------------------------------------9 1-4 使用熱治療癌症的作用機制--------------------------------------------------------10 1-5 生醫玻璃簡介 1-5-1 成份及結構------------------------------------------------------------------------13 1-5-2生醫玻璃的成份與生物活性之關係-------------------------------------------14 1-6應用於癌症熱治療之磁性顆粒------------------------------------------------------16 1-7 研究目的--------------------------------------------------------------------------------17 第二章 基本理論 2-1 磁性概述 2-1-1 磁性質------------------------------------------------------------------------------19 2-1-2 磁滯曲線與磁滯損失------------------------------------------------------------22 2-2玻璃之形成與構造 2-2-1 玻璃之形成------------------------------------------------------------------------25 2-2-2 玻璃的結構------------------------------------------------------------------------27 2-3 溶膠-凝膠技術-------------------------------------------------------------------------29 2-3-1 原料及合成------------------------------------------------------------------------30 2-3-2 凝膠的乾燥與燒結---------------------------------------------------------------31 第三章 實驗方法 3-1 實驗儀器--------------------------------------------------------------------------------33 3-2 實驗藥品--------------------------------------------------------------------------------34 3-3 材料製備--------------------------------------------------------------------------------35 3-4 材料性質分析 3-4-1 掃瞄式電子顯微鏡暨能量散佈光譜------------------------------------------37 3-4-2 感應偶合電漿/原子發射光譜--------------------------------------------------38 3-4-3 振動樣本磁量儀------------------------------------------------------------------38 3-5 材料磁滯損失測量--------------------------------------------------------------------38 3-6 生物可分解性測試--------------------------------------------------------------------42 3-7 生物相容性測試 3-7-1 MTT細胞增生測試---------------------------------------------------------------42 3-7-2 LDH細胞毒性測試---------------------------------------------------------------44 3-8 體外測試--------------------------------------------------------------------------------46 第四章 結果與討論 4-1 材料製備結果--------------------------------------------------------------------------48 4-2 掃瞄式電子顯微鏡(SEM)與能量散射光譜(EDS)分析-------------------------49 4-3 感應偶合電漿/原子發散光譜(ICP-AES)之成分分析---------------------------54 4-4 磁滯曲線分析--------------------------------------------------------------------------54 4-5 材料磁滯損失測量--------------------------------------------------------------------59 4-6生物可分解性測試---------------------------------------------------------------------63 4-7 細胞增生MTT測試-------------------------------------------------------------------65 4-8 細胞毒性LDH測試-------------------------------------------------------------------67 4-9體外測試---------------------------------------------------------------------------------69 第五章 結論-----------------------------------------------------------------------------------73 第六章 未來展望-----------------------------------------------------------------------------74 參考文獻-----------------------------------------------------------------------------------------75 圖目錄 圖1-1(a) CHO細胞在不同溫度下之生存率----------------------------------------------11 圖1-1(b) 正常組織與腫瘤組織受熱時血流量之差異比較----------------------------11 圖1-2 正常組織與腫瘤組織受熱時示意圖-----------------------------------------------12 圖1-3 熱治療與其他導致細胞死亡之生理因素-----------------------------------------12 圖2-1 順磁性物質磁化係數與溫度的關係與磁陀排列示意圖-----------------------20 圖2-2 反磁性物質的磁化強度與外加磁場關係圖--------------------------------------20 圖2-3 鐵磁性物質磁陀排列與自生磁化與溫度關係圖--------------------------------21 圖2-4 反鐵磁性物質磁陀排列和磁化係數與溫度關係圖-----------------------------21 圖2-5 陶鐵磁性物質磁陀排列和自生磁化與溫度關係圖-----------------------------22 圖2-6 鐵磁性物質之磁滯曲線--------------------------------------------------------------23 圖2-7 磁性物質(a)磁域(domain) (b)磁壁(domain wall)示意圖-----------------------24 圖2-7 磁滯曲線(c)初始磁化(d)磁化-退磁化過程之磁壁移動示意圖---------------27 圖2-8 軟磁與硬磁之磁滯曲線比較--------------------------------------------------------28 圖2-9(a) 玻璃的網狀結構--------------------------------------------------------------------27 圖2-9(b) 含修飾子的玻璃結構-------------------------------------------------------------27 圖2-10 典型的溶膠-凝膠技術流程圖-----------------------------------------------------30 圖3-1 材料製備流程--------------------------------------------------------------------------36 圖3-2 最大磁能積表示圖--------------------------------------------------------------------39 圖3-3 本實驗產生感應磁場的螺旋線圈--------------------------------------------------39 圖3-4 螺旋線圈與感應磁場-----------------------------------------------------------------40 圖3-5測量磁性生醫玻璃粉末於感應磁場中溫度上升之實驗裝置圖---------------41 圖3-6材料溫度上升與時間關係圖---------------------------------------------------------41 圖3-7 MTT與脫氫酶反應示意圖-----------------------------------------------------------43 圖3-8 LDH使tetrazolium salts反應之過程------------------------------------------------44 圖3-9 體外試驗材料與細胞於24-well中之分配圖-------------------------------------46 圖3-10 材料在感應磁場中進行體外測試之實驗裝置圖------------------------------47 圖4-1(a) 0.2 FeG的SEM型態觀察---------------------------------------------------------50 圖4-1(b) 0.2 FeG之DES元素鑑定---------------------------------------------------------50 圖4-2(a) 0.5 FeG的SEM型態觀察---------------------------------------------------------51 圖4-2(b) 0.5 FeG之DES元素鑑定----------------------------------------------------------51 圖4-3(a) 0.8 FeG的SEM型態觀察---------------------------------------------------------52 圖4-3(b) 0.8 FeG之DES元素鑑定----------------------------------------------------------52 圖4-4(a) 1.0 FeG的SEM型態觀察---------------------------------------------------------53 圖4-4(b) 1.0 FeG之DES元素鑑定----------------------------------------------------------53 圖4-5(a) 0.2 FeG的磁滯曲線----------------------------------------------------------------56 圖4-5(b) 0.5 FeG的磁滯曲線----------------------------------------------------------------56 圖4-5(c) 0.8 FeG的磁滯曲線----------------------------------------------------------------57 圖4-5(d) 1.0 FeG的磁滯曲線----------------------------------------------------------------57 圖4-6 Fe3O4內部磁偶極矩分佈示意圖----------------------------------------------------55 圖4-7(a) Fe3O4的磁滯曲線-------------------------------------------------------------------58 圖4-7(b) Fe2O3的磁滯曲線-------------------------------------------------------------------59 圖4-8 不同乾燥粉末之升溫曲線-----------------------------------------------------------60 圖4-9(a) 氧化鐵內部磁區示意圖-----------------------------------------------------------61 圖4-9(b) 磁性玻璃內部離子結構示意圖-------------------------------------------------61 圖4-10 不同粉末於水中之升溫情形------------------------------------------------------62 圖4-11(a) 0.2 FeG粉末在酸性環境下各離子釋出濃度累積圖------------------------64 圖4-11(b) 0.5 FeG粉末在酸性環境下各離子釋出濃度累積圖------------------------64 圖4-11(c) 0.8 FeG粉末在酸性環境下各離子釋出濃度累積圖------------------------65 圖4-12 不同材料分別培養1、3、7天的MTT測試---------------------------------------66 圖4-13 不同0.8 FeG濃度在培養液中之MTT測試-------------------------------------67 圖4-14 以不同材料分別培養1、3、7天之LDH測試--------------------------------68 圖4-15 不同0.8 FeG濃度在培養液中之LDH測試------------------------------------69 圖4-16(a) 0.2 FeG與細胞在磁場環境與一般環境之LDH釋放量之比較-----------70 圖4-16(b) 0.5 FeG與細胞在磁場環境與一般環境之LDH釋放量之比較-----------70 圖4-16(c) 0.8 FeG與細胞在磁場環境與一般環境之LDH釋放量之比較-----------71 表目錄 表2-1 玻璃形成物的化學特性--------------------------------------------------------------28 表3-1 生醫玻璃成分比例與原料重--------------------------------------------------------35 表3-2 含鐵重量比與實驗組簡稱-----------------------------------------------------------35 表4-1 0.2 FeG之DES元素半定量分析----------------------------------------------------50 表4-2 0.5 FeG之DES元素半定量分析----------------------------------------------------51 表4-3 0.8 FeG之DES元素半定量分析----------------------------------------------------52 表4-4 1.0 FeG之DES元素半定量分析----------------------------------------------------53 表4-5 不同鐵含量之生醫玻璃的ICP-AES定量分析(wt %)--------------------------54 表4-6 不同鐵含量之生醫玻璃的磁特性參數--------------------------------------------58 表4-7 一般氧化鐵的磁特性參數-----------------------------------------------------------59 表4-8 各種材料之SAR值比較-------------------------------------------------------------62 | |
dc.language.iso | zh-TW | |
dc.title | 可分解磁性生醫玻璃於腫瘤熱治療之研究 | zh_TW |
dc.title | The Study of Magnetic Biodegradable Bioglass for Tumor Hyperthermia | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王錫九,林睿哲,郭宗甫,蔡翠敏 | |
dc.subject.keyword | 溶膠-凝膠法,生醫玻璃,生物可分解性,磁滯損失,癌症熱治療, | zh_TW |
dc.subject.keyword | sol-gel method,bioglass,biodegradable,hysteresis loss,hyperthermia, | en |
dc.relation.page | 79 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-15 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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