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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林讚標(Tsan-Piao Lin) | |
dc.contributor.author | Mao-Sen Liu | en |
dc.contributor.author | 劉茂森 | zh_TW |
dc.date.accessioned | 2021-06-13T15:59:54Z | - |
dc.date.available | 2008-05-02 | |
dc.date.copyright | 2008-05-02 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-04-25 | |
dc.identifier.citation | Adams, R.P., Kendall, E. and Kartha, K.K. (1990) Comparison of free sugars in growing and desiccated plants of Selaginella lepidophylla. Biochem. Syst. Ecol. 18: 107-110.
Alamillo, J.M. and Bartels, D. (1996) Light and stage of development influences the expression of desiccation-induced genes in the resurrection plant Craterostigma plantagineum. Plant Cell Environ. 19: 300-310. Alpert, P. (2006) Constraints of tolerance: Why are desiccation-tolerant organisms so small or rare? J. Exp. Biol. 209: 1575-1584. Amuti, K.S. and Pollard, C.J. (1977) Soluble carbohydrates of dry and developing seeds. Phytochemistry 16: 529-532. Arrigoni, O., De Gara, L., Tommasi, F. and Liso, R. (1992) Changes in the ascorbate system during seeds development of Vicia faba L. Plant Physiol. 99: 235-238. Bartels, D. and Salamini, F. (2001) Desiccation tolerance in the resurrection plant Craterostigma plantagineum. A contribution to the study of drought tolerance at the molecular level. Plant Physiol. 127: 1346-1353. Bartels, D. and Souer, E. (2003) Molecular responses of higher plants to dehydration. In Plant response to abiotic stress, Edited by Hirt, H. and Shinozaki K. pp. 9-38. Springer-Verlag, Berlin. Bartels, D., Hanke, C., Schneider, K., Michel, D. and Salamini, F. (1992) A desiccation-related ELIP-like gene from the resurrection plant Craterostigma plantagineum is regulated by light and ABA. EMBO J. 11: 2771-2778. Beauchamp, C. and Fridovich, I. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287 Becraft, P.W. (2002) Receptor kinase signaling in plant development. Annu. Rev. Cell Dev. Biol. 18: 163-192. Bewley, J.D., Reynolds, T.L. and Oliver, M.J. (1993) Evolving strategies in the adaptation to desiccation. In Plant responses to cellular dehydration during environmental stress; current topics in plant physiology, Vol. 10. Edited by Close, T.J. and Bray, E.A. pp. 193-201. American Society of Plant Physiologists, Rockville, MD. Bianchi, G., Gamba, A., Murelli, C., Salamini, F. and Bartels, D. (1991) Novel carbohydrate metabolism in the resurrection plant Craterostigma plantagineum. Plant J. 1: 355-359. Brighigna, L., Bennici, A., Tani, C. and Tani, G. (2002) Structure and ultrastructural characterization of Selaginella lepidophylla, a desiccation-tolerant plant, during the rehydration process. Flora 197: 81-91. Buchanan, B.B., Gruissem, W. and Jones, R.L. (2000) Biochemistry & Molecular Biology of Plants. p. 486. American Society of Plant Physiologists, Rockville, Maryland. Buitink, J., Leprince, O. and Hoekstra, F.A. (2000a) Dehydration-induced redistribution of amphiphilic molecules between cytoplasm and lipids is associated with desiccation tolerance in seeds. Plant Physiol. 124: 1413-1425. Buitink, J., Mireilleet, M.A.E., Claessens, M., Marcus, A., Hemminga, A. and Hoekstra, F.A. (1998) Influence of water content and temperature on molecular mobility and intracellular glasses in seeds and pollen. Plant Physiol. 118: 531-541. Buitink, J., van den Dries, I.J., Hoekstra, F.A., Alberda, M. and Hemminga, M.A. (2000b) High critical temperature above Tg may contribute to the stability of biological systems. Biophys. J. 79: 1119-1128. Chen, J.C.F., Tsai, C.C.Y. and Tzen, J.T.C. (1999) Cloning and secondary structure analysis of caleosin, a unique calcium-binding protein in oil bodies of plant seeds. Plant Cell Physiol. 40: 1079-1086. Chen, S.Y., Chien, C.T., Chung, J.D., Yang, Y.S. and Kuo, S.R. (2007) Dormancy-break and germination in seeds of Prunus campanulata (Rosaceae): role of covering layers and changes in concentration of abscisic acid and gibberellins. Seed Sci. Res. 17: 21-32. Chen, Y. and Burris, J.S. (1991) Desiccation tolerance in maturing maize seed: membrane phospholipids composition and thermal properties. Crop Sci. 31: 766-770. Cheong, Y.H., Kim, K.N., Pandey, G.K., Gupta, R., Grant J.J. and Luan S. (2003) CBL1, a calcium sensor that differentially regulates salt, drought, and cold responses in Arabidopsis. Plant Cell 15: 1833-1845. Chin, H.F. and Roberts, E.H. (1980) Recalcitrant Crop Seeds. p. 152. Tropical Press SDN.BHD, Kuala Lumpur. Chini, A., Grant, J.J., Seki, M., Shinozaki, K. and Loake, G.J. (2004) Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1. Plant J. 38: 810-822. Chinnusamy, V., Schumaker K. and Zhu J.K. (2004) Molecular genetic perspectives on cross-talk and specificity in abiotic stress signaling in plants. J. Exp. Bot. 55: 225-236. Close, T.J. (1997) Dehydrins: a commonality in the response of plants to desiccation and low temperature. Physiol. Plant. 100: 291-296. Collett, H., Butowt, R., Smith, J., Farrant, J. and Illing, N. (2003) Photosynthetic genes are differentially transcribed during the dehydration-rehydration cycle in the resurrection plant, Xerophyta humilis. J. Exp. Bot. 54: 2593-2595. Collett, H., Shen, A., Gardner, M., Farrant, J.M., Denby, K.J. and Illing, N. (2004) Towards transcript profiling of desiccation tolerance in Xerophyta humilis: Construction of a normalized 11 k X. humilis cDNA set and microarray expression analysis of 424 cDNAs in response to dehydration. Physiol. Plant. 122: 39-53. Conn, E.E., Stumpf, P.K., Bruening, G. and Doi, R.H. (1987) Outlines of biochemistry, 5/E. p. 298. John Wiley & Sons, NY. Crowe, J.H., Crowe, L.M., Carpenter, J.F., Prestrelski, S.J., Hoekstra, F.A., de Araujo, P.S. and Panek, A.D. (1997) Anhydrobiosis: cellular adaptations to extreme dehydration. In Handbook pf Physiology Section 13, Comparative Physiology. Edited by Dantzler, W.H. pp. 1445-1477. Oxford University Press, Oxford, UK. Crowe, J.H., Hoekstra, F.A. and Crowe, L.M. (1989) Membrane phase transitions are responsible for imbibitional damage in dry pollen. P. N. A. S. USA. 86: 520-523. Crowe, J.H., Hoekstra, F.A. and Crowe, L.M. (1992) Anhydrobiosis. Annu. Rev. Physiol. 54: 579-599. Cuming, A.C., Cho, S.H., Kamisugi, Y., Graham, H. and Quatrano R.S. (2007) Microarray analysis of transcriptional responses to abscisic acid and osmotic, salt, and drought stress in the moss, Physcomitrella patens. New Phytol. 176: 275-287. Dong, H.P., Yu, H.Q., Bao, Z.L., Guo, X.Z., Peng, J.L., Yao, Z., Chen, G.Y., Qu, S.P. and Dong, H.S. (2005) The ABI2-dependent abscisic acid signalling controls HrpN-induced drought tolerance in Arabidopsis. Planta 221: 313-327. Farrant, J.M., Pammenter, N.W. and Berjak, P. (1993) Seed development in relation to desiccation tolerance: a comparison between desiccation-sensitive (recalcitrant) seeds of Avicennia marina and desiccation-tolerant types. Seed Sci. Res. 3: 1-13. Gaff, D.F. (1971) Desiccation-tolerant flowering plants in southern Africa. Science 174: 1033-1034. Gaff, D.F. and Loveys, B.R. (1984) Abscisic acid content and effects during dehydration of detached leaves of desiccation tolerant plants. J. Exp. Bot. 35: 1350-1358. Guo, Y., Xiong, L., Song, C.P., Gong, D., Halfter, U. and Zhu, J.K. (2002) A calcium sensor and its interacting protein kinase are global regulators of abscisic acid signaling in Arabidopsis. Dev. Cell 3: 233-244. Harper, J.F., Breton G. and Harmon, A. (2004) Decoding Ca2+ signals through plant protein kinases. Annu. Rev. Plant Biol. 55: 263-288. Hernandez-Pinzon, I., Patel, K. and Murphy, D.J. (2001) The Brassica napus calcium-binding protein, caleosin, has distinct endoplasmic reticulum- and lipid body-associated isoforms. Plant Physiol. Biochem. 39: 615-622. Hoekstra, F.A. and Golovina, E.A. (1999) Membrane behavior during dehydration: implications for desiccation tolerance. Russian J. Plant Physiol. 46: 295-306. Hoekstra, F.A. and Golovina, E.A. (2000) Impact of amphiphile partitioning on desiccation tolerance. In Seed Biology: Advances and Applications. Edited by Black, M., Bradford, K.J. and Vasques-Ramos, J. pp. 43-55. CAB International, Wallingford, UK. Hoekstra, F.A. and Van Roekel, T. (1988) Desiccation tolerance of Papaver dubium L. pollen during its development in the anther. Plant Physiol. 88: 626- 632. Hoekstra, F.A., Crowe, J.H. and Crowe, L.M. (1991) Effect of sucrose on phase behavior of membranes in intact pollen of Typha latifolia L., as measured with Fourier Transform Infrared Spectroscopy. Plant Physiol. 97: 1073-1079. Hoekstra, F.A., Crowe, J.H. and Crowe, L.M. (1992) Germination and ion leakage are linked with phase transitions of membrane lipids during imbibition of Typha latifolia pollen. Physiol. Plant 84: 29–34. Hoekstra, F.A., Crowe, L.M. and Crowe, J.H. (1989) Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose content. Plant Cell Enviro. 12: 83-91. Hoekstra, F.A., Golovina, E.A. and Butink, J. (2001) Mechanism of plant desiccation tolerance. Trends Plant Sci. 6: 430-438. Hoekstra, F.A., Wolkers, W.F., Butink, J., Golovina, E.A., Crowe, J.H. and Crowe, L.M. (1997) Membrane stabilization in the dry state. Comp. Biochem. Physiol. 117A: 335-341. Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols--review and survey. Seed Sci. Res. 4: 385-405. Huner, N.P.A. (1988). Low temperature-induced alteration in photosynthetic membranes. Crit. Rev. Plant Sci. 7: 257- 278. Ingle, R.A., Schmidt, U.G., Farrant, J.M., Thomson, J.A. and Mundree, S.G. (2007) Proteomic analysis of leaf proteins during dehydration of the resurrection plant Xerophyta visoca. Plant Cell Environ. 30: 435-446. Ingram, J. and Bartels, D. (1996) The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 377-403. Iturriaga, G., Cushman, M.A.F. and Cushman, J.C. (2006) An EST catalogue from the resurrection plant Selaginella lepidophylla reveals abiotic stress-adaptive genes. Plant Sci. 170: 1173-1184. Jiang, G., Wang, Z., Shang, H., Yang, W., Hu, Z., Phillips, J. and Deng, X. (2007) Proteome analysis of leaves from the resurrection plant Boea hygrometrica in response to dehydration and rehydration. Planta 225: 1405-1420. Jolivet, P., Roux, E., Andrea, S., Davanture, M., Negroni, L., Zivy, M. and Chardot, T. (2004) Protein composition of oil bodies in Arabidopsis thaliana ecotype WS. Plant Physiol. Biochem. 42: 501-509. Juaneda, P. and Rocquelin, G. (1985) Rapid and convenient separation of phospholipids and non-phosphorus lipids from rat hearts using silica cartridges. Lipids 20: 40-41. Justice, O.L. and Bass, L.N. (1979) Principles and Practices of Seed Storage. p. 289. Castle House Publications Ltd, London. Koster, K.L. (1991) Glass formation and desiccation tolerance in seeds. Plant Physiol. 96: 302-304. Lebkuecher, J.G. and Eickmeier, W.G. (1993) Physiological benefits of stem curling for resurrection plants in the field. Ecology 74: 1073-1080. Leopold, A.C., Bruni, G. and Williams, R.J. (1992) Water in dry organisms. In Water and Life. Edited by Somero, G.N., Osmond, C.B. and Bolis, C.L. pp. 161-169. Springer-Verlag, Berlin. Leprince, O., Harren, F.J.M., Buitink, J., Alberda, M. and Hoekstra, F.A. (2000) Metabolic dysfunction and unabated respiration precede the loss of membrane integrity during dehydration of germinating radicles. Plant Physiol. 122: 597-608. Leprince, O., Van Aelst, A., Pritchard, H.W. and Murphy, D.J. (1998) Oleosins prevent oil-body coalescence during seed imbibition as suggested by a low-temperature scanning electron microscope study of desiccation-tolerant and -sensitive oilseeds. Planta 204: 109-119. Li, R., Rimmer, R., Yu, M., Sharpe, A.G., Seguin-Swartz, G., Lydiate, D. and Hegedus, D.D. (2003) Two Brassica napus polygalacturonase inhibitory protein genes are expressed at different levels in response to biotic and abiotic stresses. Planta 217: 299-308. Lin, T.P. (1995) The storage behavior of several species of Fagaceae- Cyclobalanopsis gilva, C. glauca, C. morris and Quercus spinosa. Bull. Taiwan Forest Res. Inst. new series 10: 9-13. Lin, T.P. and Chien, C.T. (1995) Desiccation intolerance in seeds of six species of Machilus. Bull. Taiwan Forest Res. Inst. new series 10: 217-226. Lin, T.P. and Huang, N.H. (1994) The relationship between carbohydrate composition of some tree seeds and their longevity. J. Exp. Bot. 45: 1289-1294. Liu, M.-S., Chang, C.-Y. and Lin, T.-P. (2006) Comparison of phospholipids and their fatty acids in recalcitrant and orthodox seeds. Seed Sci. Tech. 34: 465-474. Mengiste, T., Chen, X., Salmeron, J. and Dietrich, R. (2003) The BOTRYTIS SUSCEPTIBLE1 gene encodes an R2R3MYB transcription factor protein that is required for biotic and abiotic stress responses in Arabidopsis. Plant Cell 15: 2551-2565. Minorsky, P. V. (2003) Achieving the in silico plant. Systems biology and the future of plant biological research. Plant Physiol. 132: 404-409. Morrison, W.R. and Smith, L.M. (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride methanol. J. Lipid Res. 5: 600-608. Mustilli, A.C., Merlot, S., Vavasseur, A., Fenzi, F. and Giraudat, J. (2002) Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. Plant Cell 14: 3089-3099. Neale, A.D., Blomstedt, C.K., Bronson, P., Le, T.-N., Guthridge, K., Evans, J., Gaff, D.F. and Hamill, J.D. (2000) The isolation of genes from the resurrection grass Sporobolus stapfianus which are induced during severe drought stress. Plant Cell Environ. 23: 265-277. Oliver, M.J. (1996) Desiccation tolerance in vegetative plant cells. Physiol. Plantarum 97: 779-787. Oliver, M.J. and Bewley, J. (1984) Plant desiccation and protein synthesis: VI. Changes in protein synthesis elicited by desiccation of the moss Tortula ruralis are effected at the translational level. Plant Physiol. 74: 923-927. Oliver, M.J. and Bewley, J. (1997) Desiccation tolerance of plant tissues: a mechanistic overview. Hort. Rev. 18: 171-214. Oliver, M.J., Tuba, Z. and Mishler, B.D. (2000) The evolution of vegetative desiccation tolerance in land plants. Plant Ecol.151: 85-100. Oliver, M.J., Velten, J. and Mishler, B.D. (2005) Desiccation tolerance in bryophytes: a reflection of the primitive strategy for plant survival in dehydration habitats? Integr. Comp. Biol. 45: 788-799. Ouvrard, O., Cellier, F., Ferrare, K., Tousch, D., Lamaze, T., Dupuis, J.-M. and Casse-Delbart, F. (1996) Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype. Plant Mol. Biol. 31: 819-829. Pan, S.M., Hwang, G.B. and Liu, H.C. (1999) Over-expression and characterization of copper/zinc-superoxide dismutase from rice in Escherichia coli. Bot. Bull. Acad. Sin. 40: 275-281 Platt, K.A. and Thomson, W.W. (1997) Conservation of cell order in desiccated mesophyll of Selaginella lepidophylla ([Hook and Grev.]Spring). Ann. Bot. 79: 439-447. Porembski, S. and Barthlott, W. (2000) Granitic and gneissic outcrops (inselbergs) as centers of diversity for desiccation-tolerant vascular plants. Plant Ecol. 151: 19-28. Poxleitner, M., Rogers, S.W., Samuels, A.L., Browse, J. and Rogers, J.C. (2006) A role for caleosin in degradation of oil-body storage lipid during seed germination. Plant J. 47: 917-933. Proctor, M.C.F. and Pence, V.C. (2002) Vegetative tissues: bryophytes, vascular resurrection plants, and vegetative propogules. In Desiccation and survival in plants: drying without dying. Edited by Black, M. and Pritchard, H.W. pp. 207-237. Oxon: CABI Publishing, Wallingford, UK. Pukacka, S. (1999) Membrane phospholipids composition during maturation of seeds of Acer platanoides and Acer pseudoplatanus in relation to desiccation tolerance. Acta Physiol. Plantarum 21: 109-115. Quartacci, M.F., Forli, M., Rascio, N., Dalla Vecchia, F., Bochicchio, A. and Navari-Izzo, F. (1997) Desiccation-tolerant Sporobolus stafinaus: lipid composition and cellular ultrastructure during dehydration and rehydration. J. Exp. Bot. 48: 1269-1279. Quartacci, M.F., Glišić, O., Stevanović, B. and Navari-Izzo, F. (2002) Plasma membrane lipids in the resurrection plant Ramonda serbica following dehydration and rehydration. J. Exp. Bot. 53: 2159-2166. Quinn, P.J. (1985) A lipid-phase separation model of low temperature damage to biological membrane. Cryobiology 22: 128-146. Reddy, V.S. and Reddy, A.S.N. (2004) Proteomics of calcium-signaling components in plants. Phytochemistry 65: 1745-1776. Robert, R., Strewart, C. and Bewley, J.D. (1982) Stability and synthesis of phospholipids during desiccation and rehydration of a desiccation-tolerant and a desiccation-intolerant moss. Plant Physiol. 69: 724-727. Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Sci. Technol. 1: 499-514. Sakuma, Y., Maruyama, K., Osakabe, Y., Qin, F., Seki, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2006) Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18: 1292-1309. Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., et al. (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold, and high-salinity stresses using a full-length cDNA microarray. Plant J. 31: 279-292. Senaratna, T., McKersie, B.D. and Stinson, R.H. (1984) Association between membrane phase properties and dehydration injury in soybean axes. Plant Physiol. 76: 759- 762. Siedow, J.N. and Day, D.A. (2000) Respiration and photorespiration. In Biochemistry and molecular biology of plants. Edited by Buchanan, B.B., Gruissem, W. and Jones, R.L. pp. 676-728. American Society of Plant Physiologists, Rockville, MD. Smirnoff, N. (1992) The carbohydrates of bryophytes in relation to desiccation-tolerance. J. Bryol. 17: 185-191. Song, L., Ding, W., Zhao, M., Sun, B. and Zhang, L. (2006) Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed. Plant Sci. 171: 449-458. Stanislawa, P. (1999) Membrane phospholipid composition during maturation of seeds of Acer platanoides and Acer pseudoplatanus in relation to desiccation tolerance. Acta Physiol. Plant. 21: 109-115. Steponkus, P.L., Lynch, D.V. and Uemura, M. (1990) The influence of cold acclimation on the lipid composition and cryobehaviour of the plasma membrane of isolated rye protoplasts. Phil. Trans. Royal Society, London B 326: 571-583. Sun, W.Q., Irving, T.C. and Leopold, A.C. (1994) The role of sugar, vitrification and membrane phase transition in seed desiccation tolerance. Physiol. Plantarum, 90: 621-628. Tetteroo, F.A.A., de Bruijn, A.Y., Henselmans, R.N.M., Wolkers, W.F. and van Aelst, A.C. (1996) Characterization of membrane properties in desiccation-tolerant and -intolerant carrot somatic embryos. Plant Physiol. 111: 403–412. Tsai, J.L. and Shieh, W.C. (1994) Selaginellaceae. In Flora of Taiwan, Vol. I. Second edition. Edited by Editorial Committee of the Flora of Taiwan, p 56. Editorial Committee of the Flora of Taiwan, Taipei, Taiwan. Uemura, M. and Steponkus, P.L. (1989) Effect of cold acclimation on the increase of two forms of freezing injury in protoplasts isolated from rye leaves. Plant Physiol. 91: 1131-1137. Van Ooijen, G., van den Burg, H.A., Cornelissen, B.J.C. and Takken, F.L.W. (2007) Structure and function of resistance proteins in solanaceous plants. Annu. Rev. Phytopathol. 45: 43-72. Wang, B.S.P., Lin, T.P. and Chien, C.T. (1995) Classification of storage behaviour of forest tree seeds. Bull. Taiwan Forest Res. Inst. new series 10: 255-276. Wang, W., Vinocur, B. and Altman, A. (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1-14. Xiong, L., Schumaker, K.S. and Zhu, J.K. (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14 suppl: S165-183. Zeng, Q., Chen, X. and Wood, A.J. (2002) Two early light-inducible protein (ELIP) cDNAs from the resurrection plant Tortula ruralis are differentially expressed in response to desiccation, rehydration, salinity, and high light. J. Exp. Bot. 53: 1197-1205. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/38069 | - |
dc.description.abstract | 細胞膜磷脂質的組成在種子適應乾燥逆境扮演重要角色。因此,我們分析了許多種乾儲型和異儲型種子在胚軸的磷脂組成。結果顯示,這兩類種子的胚軸在phosphatidyl ethanolamine (PE)和phosphatidylcholine (PC)的比值有明顯的重疊。在磷脂的脂肪酸飽和度上,乾儲型種子飽和脂肪酸佔的比例(16% to 27%)明顯低於異儲型種子(32% to 36%),這個差異也反映在細胞膜的相變溫度上,而兩類種子在非飽和脂肪酸的比例上則無顯著差異。在以萌發的大豆種子模擬異儲型種子的實驗中,PE和PC的比值先增後減。萌芽後失去耐乾燥能力的胚軸在磷脂脂肪酸飽和度上明顯增加。這個結果與乾、異儲型種子的比較結果相符。然而,飽和度的增加並未反映在萃取出來的磷脂的相變溫度上,這可能是linolenic acids所造成的影響。總之,不耐乾燥的異儲型種子和萌發的大豆胚軸具有較高的磷脂飽和度,較高含量的linolenic acids與較高的細胞膜相變溫度。
萬年松是最原始的維管束植物之一,它可以在乾燥下存活,當重新獲得水分時恢復生機。為了了解萬年松忍受乾燥的特性,我們利用氣相層析儀分析乾燥前後萬年松在可溶性醣和磷脂飽和度上的差異。此外,我們也分析了萬年松在乾燥過程蛋白質、超氧歧化酵素活性、基因表現和ABA含量的變化。結果顯示海藻糖是萬年松主要的可溶性醣(以乾重計算,每克萬年松含量超過130毫克),其含量在乾燥前後無明顯差異。乾燥前、後的植物組織在細胞膜磷脂上皆維持低的脂肪酸飽和度(0.31)。乾燥過程中可以看到新的蛋白質被合成與新的超氧歧化酵素活性被活化。此外,乾燥過程ABA含量增加三倍,ABA訊息傳遞和細胞保護相關基因被誘導表現,光合作用相關基因的表現則被抑制。由這些生化和分子生物上的證據顯示持續性的高雙醣含量和低磷脂飽和度與乾燥誘導ABA增加和基因表現皆參與了萬年松忍受乾燥的機制。 我們利用differential display的方式,在萬年松篩選到一個受缺水誘導表現的基因,StCaLB。此基因的產物是一個calcium dependent lipid binding蛋白。為了進一步研究此基因的功能,我們分析了這個基因在不同非生物性逆境下的表現和protein domain的預測,並將此基因的cDNA轉入阿拉伯芥中持續性的表達。結果顯示,StCaLB的表現會受到鹽、30% PEG、熱和ABA的誘導但不受低溫處理影響。蛋白質domain的預測顯示,StCaLB在N端有兩個transmembrane domain,在蛋白質中段有一個C2 domain。另外我們也得到StCaLB持續性表達的阿拉伯芥轉植株。然而,StCaLB的功能仍有賴更進一步的生化與遺傳方面的分析。 | zh_TW |
dc.description.abstract | Membrane phospholipids play an important role in acclimation of seeds to desiccation stresses. The compositions of phospholipids in embryonic axes of several recalcitrant seeds and orthodox seeds were studied. The ratio of phosphatidyl ethanolamine to phosphatidylcholine in embryonic axes of recalcitrant seeds and orthodox seeds was overlapping in the range. Percentage of saturated fatty acids of phospholipid (stearic acid, C18:0, and palmitic acid, C16:0) was significantly different between orthodox seeds (in the range of 16% to 27%) and recalcitrant seeds (32% to 36%), and this difference also reflected in the lower phase transition temperature of orthodox seeds. The proportion of unsaturated fatty acids (C18:2 and C18:3) of phospholipids was overlapping in both seed types. Using imbibed soybean seedlings as a model system for recalcitrance, the ratio of phosphatidyl ethanolamine over phosphatidylcholine of embryonic axes increased in early imbibition and decreased later on. The percentage of saturated fatty acids in phospholipids of soybean seedlings excluding cotyledon was significantly higher than that in dry embryonic axis. This agrees with the results from the comparison of recalcitrant and orthodox seeds. Increases in saturated fatty acids were not correlated with lower transition temperature of extracted phospholipids of seedling indicating linolenic acids (C18:3) might play a role on Tm value. In conclusion, both recalcitrant seeds and imbibed seedlings while sharing recalcitrance, exhibited a similar trend in fatty acid saturation and higher percentage of linolenic acid in phospholipids, and higher phase transition temperature when compared with orthodox seeds and dry embryonic axes.
Selaginella tamariscina, one of the most primitive vascular plants, can remain alive in a desiccated state and resurrect when water becomes available. To evaluate the nature of desiccation tolerance in this plant, we compared the composition of soluble sugars and saturation ratios of phospholipids (PLs) between hydrated and desiccated tissues of S. tamariscina using gas chromatography (GC). In this study, differences in protein dynamics, superoxide dismutase (SOD) activities, gene expressions and abscisic acid (ABA) contents were also analyzed during dehydration. The results revealed that trehalose (at > 130 mg g-1 dry weight) was the major soluble sugar, and low saturated fatty acid contents in PL (0.31) was maintained in both hydrated and desiccated tissues. Novel proteins and an inducible CuZnSOD activity were detected during dehydration. In addition, the ABA content of S. tamariscina increased 3 folds, and genes involved in ABA signaling and cellular protection were upregulated while photosystem-related genes were downregulated during dehydration. The biochemical and molecular findings suggest that both constitutive and inducible protective molecules contribute to desiccation tolerance of S. tamariscina. To study the function of StCaLB putatively encoding a calcium dependent lipid binding protein screened from differential display of Selaginella tamariscina during dehydration, the genomic sequence, putative protein domain and the response of this gene to different abiotic stresses were analyzed. Also, the transgenic plants of Arabidopsis that over-expressing StCaLB were generated. The results indicated the expression of StCaLB is induced by NaCl, 30% PEG, heat and abscisic acid, but not by cold treatment. The StCaLB is predicted to contain two N-terminal transmembrane domains and a central C2 domain. The StCaLB over-expressing Arabidopsis were obtained. However, further biochemistry and genetic research still need to be evaluated for the function of this gene. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:59:54Z (GMT). No. of bitstreams: 1 ntu-97-D89226001-1.pdf: 1867971 bytes, checksum: 9d14fd11ace0191a2a67d69cbe48e382 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | Table of Contents
Page Abbreviations…………………………………………………...…………………….1 Chapter 1: Comparison of phospholipids and their fatty acids in recalcitrant and orthodox seeds…………………………………………………………………………3 Abstract in Chinese…………………………………………………………………..3 Abstract in English……………………………………………...……………………4 Introduction…………………………………………………………………………..6 Materials and Methods………………………………………………………………8 Plant materials………………………………………………………………………..8 Soybean seed germination and treatment…………………………………………….9 Lipid extraction and analysis………………………………………………………..10 Phospholipid analysis……………………………………………………………….10 Fatty acid analysis..…………………………………………………………………11 Fourier transform infrared spectroscopy (FTIR)..…………………………………..12 Results……………………………………………………….……………………….13 Composition of PL classes in various seeds………………………………………...14 Weight percentage of fatty acids in PL of recalcitrant and orthodox seed….............14 Tm for embryonic tissue of recalcitrant seed and orthodox seed……………...…….15 Changes of composition of PL and their fatty acids of soybean seeds imbibed for up to 32 h at 25 oC………………………………..…………………………………….15 Tm for imbibed seedlings and extracted PLs of soybean……………………………16 Discussion……………………………………………………………………………16 Figures and tables…………………………………………………………………...22 Chapter 2: Constitutive components and induced gene expressions are involved in the desiccation tolerance of Selaginella tamariscina…………………...……………30 Abstract in Chinese…………………………………………………………………30 Abstract in English………………….………………………………………………31 Introduction…………………………………………………………………………32 Materials and Methods……………………………………………………………..35 Plant materials………………………………………………………………………35 Water content determination……………………………………….……………….36 Ion leakage analysis…………………….…………………………………………..36 Extraction and analysis of phospholipids (PLs) and soluble sugars….…………….37 Extraction, purification, and quantification of ABA……………………….……….38 Protein extraction and two dimensional protein analysis………….………………..40 SOD activity analyses………………………………………………………………41 Dehydration activated cDNA clone isolation and DNA sequence analysis….….….42 Selection of differentially expressed genes during dehydration of S. tamariscina....42 Northern blot analysis of gene expression in dehydration- and ABA-treated S. tamariscina…………………………………………………………………….…..44 Results………………………………………………………….……………………45 Resurrection phenomenon of S. tamariscina……………………………………….45 Low saturation ratio of fatty acids in PLs was maintained during dehydration……46 Trehalose was the main soluble sugar detected in S. tamariscina…………….……47 ABA increased in dehydrated S. tamariscina……………………………………….47 Novel proteins were synthesized in desiccated S. tamariscina………………..……48 CuZn-SOD activities were activated during dehydration in S. tamariscina………..48 Eighteen unique genes were upregulated during dehydration in S. tamariscina…...49 A calcium dependent lipid binding protein gene and a hydrolase gene were specifically expressed during dehydration…………………….……………………49 Twenty-two unique genes were differentially expressed between hydrated and dehydrated S. tamariscina……………….……………………………………….…49 ABA-dependent gene expressions were revealed in S. tamariscina…….……….…51 Discussion……………………………………………………………………………52 The membrane system of S. tamariscina was well protected during desiccation and recruit of water…..………………………………………………………………….52 Low saturation of PLs in membranes was found in S. tamariscina……………...…53 Accumulation of a high level of disaccharide is a characteristic of lower vascular plants………………………………………………………………………………..54 An inducible protective mechanism is involved in acquisition of desiccation tolerance in S. tamariscina………………………………………………………….56 Figures and tables………………..………………………………………………….62 Chapter 3: A gene encodes a putative calcium dependent lipid binding protein was response to diverse abiotic stresses in Selaginella tamariscina…………………..….78 Abstract in Chinese…………………………………………………………………78 Abstract in English………………….………………………………………………79 Introduction…………………………………………………………………………80 Materials and Methods……………………………………………………………..80 Plant materials………………………………………………………………………80 Northern blot analysis of gene expression under different abiotic stresses…….…..81 Gene construction and plant transformation………………………………………..81 Results………………………………………………………….……………………82 The cloning and expression pattern of the putative calcium dependent lipid binding protein gene of S. tamariscina…………………………………….……..………….82 StCaLB overexpressed in Arabidopsis….……………………….………………..…83 Discussion……………………………………………………………………………83 Figures and tables………………..………………………………………………….85 References………………………………………………………………………...…89 Appendix: Nucleotide sequence of differentially expressed genes identified in S. tamariscina…………………………………………………………………....….....105 | |
dc.language.iso | en | |
dc.title | 植物耐乾燥之研究:從種子生理到營養組織之分子機制 | zh_TW |
dc.title | Plant desiccation tolerance: from physiology of seeds to the molecular mechanism of vegetative tissues | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 馮騰永(Teng-Yung Feng),張孟基(Men-Chi Chang),謝明勳(Ming-Hsiun Hsieh),謝旭亮(Hsu-Liang Hsieh),鄭石通(Shih-Tong Jeng),王國祥(Co-Shine Wang),吳克強(Keqiang Wu) | |
dc.subject.keyword | 種子,磷脂,萬年松,乾燥,離層酸,海藻糖,鈣, | zh_TW |
dc.subject.keyword | seeds,phospholipids,Selaginella tamariscina,desiccation,abscisic acid,trehalose,calcium, | en |
dc.relation.page | 131 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-04-28 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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