請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37962
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 閔明源(Ming-Yuan Min) | |
dc.contributor.author | Tien-Wei Chang | en |
dc.contributor.author | 張天偉 | zh_TW |
dc.date.accessioned | 2021-06-13T15:53:38Z | - |
dc.date.available | 2014-08-19 | |
dc.date.copyright | 2011-08-19 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-09 | |
dc.identifier.citation | Aston-Jones G, Cohen JD (2005) An integrative theory of locus coeruleus-norepinephrine function: adaptive gain and optimal performance. Annu Rev Neurosci 28:403-450.
Atluri PP, Regehr WG (1998) Delayed release of neurotransmitter from cerebellar granule cells. J Neurosci 18:8214-8227. Bajic D, Van Bockstaele EJ, Proudfit HK (2001) Ultrastructural analysis of ventrolateral periaqueductal gray projections to the A7 catecholamine cell group. Neuroscience 104:181-197. Burnett A, Gebhart GF (1991) Characterization of descending modulation of nociception from the A5 cell group. Brain Res 546:271-281. Byrum CE, Guyenet PG (1987) Afferent and efferent connections of the A5 noradrenergic cell group in the rat. J Comp Neurol 261(4):529-542 Clark FM, Proudfit HK (1991a) The projection of noradrenergic neurons in the A7 catecholamine cell group to the spinal cord in the rat demonstrated by anterograde tracing combined with immunocytochemistry. Brain Res 547:279-288. Clark FM, Proudfit HK (1991b) Projections of neurons in the ventromedial medulla to pontine catecholamine cell groups involved in the modulation of nociception. Brain Res 540:105-115. Cohen IS, Van der Kloot W (1986) Facilitation and delayed release at single frog neuromuscular junctions. J Neurosci 6:2366-2370. Dahlstroem A, Fuxe K (1964) Evidence for the Existence of Monoamine-Containing Neurons in the Central Nervous System. I. Demonstration of Monoamines in the Cell Bodies of Brain Stem Neurons. Acta Physiol Scand Suppl SUPPL 232:231-255. Dias QM, Crespilho SF, Silveira JW, Prado WA (2009) Muscarinic and alpha(1)-adrenergic mechanisms contribute to the spinal mediation of stimulation-induced antinociception from the pedunculopontine tegmental nucleus in the rat. Pharmacol Biochem Behav 92:488-494. Gilmore ML, Nash NR, Roghani A, Edwards RH, Yi H, Hersch SM, Levey AI (1996) Expression of the putative vesicular acetylcholine transporter in rat brain and localization in cholinergic synaptic vesicles. J Neurosci 16:2179–2190 Holden JE, Schwartz EJ, Proudfit HK (1999) Microinjection of morphine in the A7 catecholamine cell group produces opposing effects on nociception that are mediated by alpha1- and alpha2-adrenoceptors. Neuroscience 91:979-990. Holden JE, Van Poppel AY, Thomas S (2002) Antinociception from lateral hypothalamic stimulation may be mediated by NK(1) receptors in the A7 catecholamine cell group in rat. Brain Res 953:195-204. Iremonger KJ, Bains JS (2007) Integration of asynchronously released quanta prolongs the postsynaptic spike window. J Neurosci 27:6684-6691. Iwamoto ET (1989) Antinociception after nicotine administration into the mesopontine tegmentum of rats: evidence for muscarinic actions. J Pharmacol Exp Ther 251:412-421. Iwamoto ET, Marion L (1993) Adrenergic, serotonergic and cholinergic components of nicotinic antinociception in rats. J Pharmacol Exp Ther 265(2):777-789. Jensen TS, Yaksh TL (1986) Examination of spinal monoamine receptors through which brainstem opiate-sensitive systems act in the rat. Brain Res 363:114-127. Katayama Y, Watkins LR, Becker DP, Hayes RL (1984) Non-opiate analgesia induced by carbachol microinjection into the pontine parabrachial region of the cat. Brain Res 296:263-283. Kwiat GC, Basbaum AI (1992) The origin of brainstem noradrenergic and serotonergic projections to the spinal cord dorsal horn in the rat. Somatosens Mot Res 9:157-173. Levitt P, Moore RY (1979) Origin and organization of brainstem catecholamine innervation in the rat. J Comp Neurol 186:505-528. Ma HC, Dohi S, Wang YF, Ishizawa Y, Yanagidate F (2001) The antinociceptive and sedative effects of carbachol and oxycodone administered into brainstem pontine reticular formation and spinal subarachnoid space in rats. Anesth Analg 92:1307-1315. Mesulam MM, Mufson EJ, Wainer BH, Levey AI (1983) Central cholinergic pathways in the rat: an overview based on an alternative nomenclature (Ch1-Ch6). Neuroscience 10:1185-1201. Millan MJ (2002) Descending control of pain. Prog Neurobiol 66:355-474. Miller JF, Proudfit HK (1990) Antagonism of stimulation-produced antinociception from ventrolateral pontine sites by intrathecal administration of alpha-adrenergic antagonists and naloxone. Brain Res 530:20-34. Min MY, Hsu PC, Lu HW, Lin CJ, Yang HW (2007) Postnatal development of noradrenergic terminals in the rat trigeminal motor nucleus: A light and electron microscopic immunocytochemical analysis. Anat Rec (Hoboken) 290:96-107. Min MY, Wu YW, Shih PY, Lu HW, Lin CC, Wu Y, Li MJ, Yang HW (2008) Physiological and morphological properties of, and effect of substance P on, neurons in the A7 catecholamine cell group in rats. Neuroscience 153:1020-1033. Min MY, Wu YW, Shih PY, Lu HW, Wu Y, Hsu CL, Li MJ, Yang HW (2010) Roles of A-type potassium currents in tuning spike frequency and integrating synaptic transmission in noradrenergic neurons of the A7 catecholamine cell group in rats. Neuroscience 168:633-645. Moore RY, Bloom FE (1979) Central catecholamine neuron systems: anatomy and physiology of the norepinephrine and epinephrine systems. Annu Rev Neurosci 2:113-168. Olmstead MC, Munn EM, Franklin KB, Wise RA (1998) Effects of pedunculopontine tegmental nucleus lesions on responding for intravenous heroin under different schedules of reinforcement. J Neurosci 18:5035-5044. Panatier A, Gentles SJ, Bourque CW, Oliet SH (2006) Activity-dependent synaptic plasticity in the supraoptic nucleus of the rat hypothalamus. J Physiol 573:711-721. Peng YB, Lin Q, Willis WD (1996) Involvement of alpha-2 adrenoceptors in the periaqueductal gray-induced inhibition of dorsal horn cell activity in rats. J Pharmacol Exp Ther 278:125-135. Pertovaara A (2006) Noradrenergic pain modulation. Prog Neurobiol 80:53-83. Rahamimoff R, Yaari Y (1973) Delayed release of transmitter at the frog neuromuscular junction. J Physiol 228:241-257. Schaffer MK, Eiden LE, Weihe E (1998) Cholinergic neurons and terminal fields revealed by immunohistochemistry for the vesicular acetylcholine transporter. I. Central nervous system. Neuroscience 84, 331–359. Shi TJ, Winzer-Serhan U, Leslie F, Hokfelt T (1999) Distribution of alpha2-adrenoceptor mRNAs in the rat lumbar spinal cord in normal and axotomized rats. Neuroreport 10:2835-2839. Sofroniew MV, Priestley JV, Consolazione A, Eckenstein F, Cuello AC (1985) Cholinergic projections from the midbrain and pons to the thalamus in the rat, identified by combined retrograde tracing and choline acetyltransferase immunohistochemistry. Brain Res 329:213-223. Stone LS, Broberger C, Vulchanova L, Wilcox GL, Hokfelt T, Riedl MS, Elde R (1998) Differential distribution of alpha2A and alpha2C adrenergic receptor immunoreactivity in the rat spinal cord. J Neurosci 18:5928-5937. Thakkar MM, Strecker RE, McCarley RW (1998) Behavioral state control through differential serotonergic inhibition in the mesopontine cholinergic nuclei: a simultaneous unit recording and microdialysis study. J Neurosci 18:5490-5497. Wadel K, Neher E, Sakaba T (2007) The coupling between synaptic vesicles and Ca2+ channels determines fast neurotransmitter release. Neuron 53:563-575. Wang HL, Morales M (2009) Pedunculopontine and laterodorsal tegmental nuclei contain distinct populations of cholinergic, glutamatergic and GABAergic neurons in the rat. Eur J Neurosci 29:340-358. Weihe E, Tao-Cheng JH, Schafer MK, Erickson JD, Eiden LE (1996) Visualization of the vesicular acetylcholine transporter in cholinergic nerve terminals and its targeting to a specific population of small synaptic vesicles. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3547-52. Westlund KN, Craig AD (1996) Association of spinal lamina I projections with brainstem catecholamine neurons in the monkey. Exp Brain Res 110:151-162. Winn P (2006) How best to consider the structure and function of the pedunculopontine tegmental nucleus: evidence from animal studies. J Neurol Sci 248:234-250. Woolf NJ, Butcher LL (1986) Cholinergic systems in the rat brain: III. Projections from the pontomesencephalic tegmentum to the thalamus, tectum, basal ganglia, and basal forebrain. Brain Res Bull 16:603-637. Wu Y, Wang HY, Lin CC, Lu HC, Cheng SJ, Chen CC, Yang HW, Min MY (2011) GABAB receptor-mediated tonic inhibition of noradrenergic A7 neurons in the rat. J Neurophysiol 105:2715-2728. Yeomans DC, Clark FM, Paice JA, Proudfit HK (1992) Antinociception induced by electrical stimulation of spinally projecting noradrenergic neurons in the A7 catecholamine cell group of the rat. Pain 48:449-461. Yeomans DC, Proudfit HK (1992) Antinociception induced by microinjection of substance P into the A7 catecholamine cell group in the rat. Neuroscience 49:681-691. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37962 | - |
dc.description.abstract | 腳橋被蓋核區(pedunculopontine tegmental nucleus, PPTg)是一包含膽鹼性(cholinergic)、麩胺酸性(glutamatergic)等其他種類之神經元投射至許多區域的核區。在過去的動物行為實驗研究指出,在腳橋被蓋核給予電刺激或化學性刺激之後可以引發出鎮痛反應。而在前人研究結果也指出A7正腎上腺素神經元(NAergic A7 neurons)上擁有膽鹼性受器,A7正腎上腺素神經元已有文獻證實會投射軸突至脊髓背角(dorsal horn),分泌正腎上腺素來調控痛覺傳遞。根據以上結果,我們假設A7正腎上腺素神經元接受來自腳橋被蓋核區之膽鹼性神經投射來調控下游之痛覺傳遞,並由型態方面及電生理方面進行實驗來探討兩者之間連結是否存在及其特性。經由腦定位儀手術將BDA正向神經追蹤劑注射至腳橋被蓋核區,經由免疫染色標定後觀察其投射之神經纖維和A7細胞群。在型態方面的實驗結果可以看到來自腳橋被蓋核被BDA標定之軸突纖維往A7細胞群延伸並形成似突觸之結構。而電生理實驗方面,在腳橋被蓋核予以單一電刺激可在A7細胞群引發一內向電流,同時施給木防已苦毒素(picrotoxin)和士的寧(strychnine)可以部份抑制此內向電流,顯示在此神經投射當中具有抑制性(inhibitory)神經纖維。高頻率電刺激則可在A7細胞群記錄到一可被蕈毒膽鹼性受器(mAChRs)拮抗劑-阿托品(atropine)部分抑制之突觸後興奮性電流,顯示在此神經投射中也具有膽鹼性的神經纖維。在此高頻刺激之後,也在A7細胞群此處記錄到麩胺酸性的非同步釋放(asynchronous release)的現象。在此神經投射也發現了自主回饋抑制(auto-inhibition)的調控現象。這些實驗結果證明從腳橋被蓋核區往A7正腎上腺素神經元的投射的確存在,也顯示出在此神經投射當中包含有抑制性,膽鹼性,以及麩胺酸性的神經纖維。此實驗結果也對於脊髓以上蕈毒膽鹼性系統與正腎上腺素之下行性痛覺調控路徑的交互作用提供了有利之證據。 | zh_TW |
dc.description.abstract | Pedunculopontine tegmental nucleus (PPTg) is a nucleus containing cholinergic neurons, glutamatergic neurons, and other kinds of neurons projecting toward many regions. Many behavioral studies have shown that electrical or chemical stimulation at PPTg can induce antinociception response. Also previous studies have shown that there are cholinergic receptors on NAergic A7 cell group, which projects NAergic fibers to dorsal horn to modulate nociceptive signaling. From the above results, we hypothesized that NAergic A7 neurons receive cholinergic projection from PPTg to modulate descending nociceptive signaling. The projection from PPTg to NAergic A7 neurons was investigated by morphological and electrophysiological methods. BDA anterograde tracer was injected in PPTg by stereotaxic surgery, and the A7 neurons and the projection fibers from PPTg were labeled by immunohistochemistry. In the morphological results, labeled axon fibers spread toward to NAergic A7 neurons forming synaptic terminal contact-like structures. In electrophysiological experiments, single pulse at PPTg was used to evoke an inward current which could be partial blocked by co-application of picrotoxin (antagonist of GABAA receptors) and strychnine (antagonist of glycine receptors), suggesting there are inhibitory components in this projection. High frequency stimulation at PPTg evoked an inward current partially blocked by muscarinic antagonist, atropine, suggesting that cholinergic fibers are also involved in this projection. Moreover, calcium-dependent asynchronous glutamate release events were also found on A7 neurons in this experiment. There was an auto-inhibition in the cholinergic system between A7 and PPTg transmission. These results provide an evidence for the existence of the projection from PPTg toward NAergic A7 neurons, and there are least inhibitory, glutamatergic, and cholinergic fibers involved in this projection. The results in this study also provide an evidence for supraspinal interaction between muscarinic cholinergic system and NAergic descending pain pathway. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:53:38Z (GMT). No. of bitstreams: 1 ntu-100-R98b41026-1.pdf: 38677496 bytes, checksum: 874c48f24f3ce450cc7a6c45978f485b (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 致謝.......................................................i
摘要....................................................iiii ABSTRACT...................................................v INTRODUCTION...............................................1 Noradrenergic (NAergic) system in the central nervous system.....................................................1 A7 catecholamine cell group involved in descending pain modulating pathway.........................................2 The cholinergic system in the central nervous system.......4 The role of pedunculopontine tegmental nucleus (PPTg) and muscarinic acetylcholine receptors (mAChRs) in pain modulation.................................................5 Aim........................................................7 MATERIALS AND METHODS......................................8 BDA anterograde tracing and immunohitochemistry............8 Stereotactic surgery.......................................8 Immunohistochemistry.......................................9 Electrophysiology.........................................11 Preparation of brian stem slices..........................11 Whole-cell patch clamp recording..........................12 Data acquisition and analysis.............................14 Drugs.....................................................15 Filling recorded neurons with biocytin and immunohistochemistry......................................16 RESULTS...................................................17 BDA anterograde tracing and immunohistochemistry..........17 Identification of the BDA injection sites on Pedunculopontine tegmental nucleus (PPTg).................17 The projection fibers from PPTg had distribution to A7 NAergic neurons area......................................18 Electrophysiologiy........................................21 Identification of A7 NAergic cell groups in rat brainstem slices....................................................21 Stimulation at Pedunculopontine tegmental nucleus (PPTg) evoked excitatory postsynaptic currents (EPSCs) on A7 NAergic neurons...........................................22 There are inhibitory and glutamatergic components in the projection from PPTg to NAergic A7 neurons................23 High frequency stimulation at PPTg could evoke complex EPSCs on A7 neurons.............................................24 Glutamatergic asynchronous release could be found at PPTg-A7 transmission..............................................25 EGTA-AM could inhibited this evoked asynchronous glutamatergic release.....................................26 Asynchronous release could be potentiated by increasing the number of pulses..........................................27 There is a mAChRs mediated auto-inhibition in PPTg-A7 transmission..............................................27 DISCUSSION................................................29 BDA anterograde tracing and immunohistochemistry..........30 Pedunculopontine tegmental nucleus (PPTg) projects inhibitory, glutamatergic and cholinergic axon fibers to NAergic A7 neurons........................................32 The glutamatergic asynchronous release exists in the synaptic transmission from PPTg to NAergic A7 neurons.....33 There is a mAChRs-mediated auto-inhibition in PPTg-A7 transmission..............................................35 Physiological implication of the projection from PPTg to NAergic A7 neurons........................................36 REFERENCES................................................38 FIGURES...................................................43 | |
dc.language.iso | en | |
dc.title | 大鼠腳橋被蓋核至A7核區正腎上腺素神經元
之膽鹼性及麩胺酸性投射機制與特徵 | zh_TW |
dc.title | Cholinergic and Glutamatergic projection from Pedunculopontine Tegmental Nucleus to Noradrenergic A7 Cell Group in Rats | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳瑞芬(Ruei-Feng Chen),楊琇雯(Hsiu-Wen Yang) | |
dc.subject.keyword | 腦幹,A7核區,全細胞電生理紀錄,乙醯膽鹼,蕈毒膽鹼性受器,腳橋被蓋核,痛覺傳導, | zh_TW |
dc.subject.keyword | brainstem,NE,A7 cell group,whole-cell recording,ACh,mAChR,PPTg,asynchronous release,synaptic transmission, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-10 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 37.77 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。