請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37684
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄧麗珍 | |
dc.contributor.author | Hong-Sih Jiang | en |
dc.contributor.author | 江紅思 | zh_TW |
dc.date.accessioned | 2021-06-13T15:38:33Z | - |
dc.date.available | 2014-10-07 | |
dc.date.copyright | 2011-10-07 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-10 | |
dc.identifier.citation | 1. Lowy, F.D. Staphylococcus aureus infections. N Engl J Med, 1998. 339: p. 520-32.
2. Lyon, B.R. and R. Skurray. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev, 1987. 51: p. 88-134. 3. Pantosti, A. and M. Venditti. What is MRSA? Eur Respir J, 2009. 34: p. 1190-6. 4. Leski, T.A. and A. Tomasz. Role of penicillin-binding protein 2 (PBP2) in the antibiotic susceptibility and cell wall cross-linking of Staphylococcus aureus: evidence for the cooperative functioning of PBP2, PBP4, and PBP2A. J Bacteriol, 2005. 187: p. 1815-24. 5. Goffin, C. and J.M. Ghuysen. Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol Mol Biol Rev, 1998. 62: p. 1079-93. 6. Katayama, Y., T. Ito and K. Hiramatsu. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother, 2000. 44: p. 1549-55. 7. Pinho, M.G., H. de Lencastre and A. Tomasz, An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. Proc Natl Acad Sci U S A, 2001. 98: p. 10886-91. 8. Hartman, B.J. and A. Tomasz. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol, 1984. 158: p. 513-6. 9. Diekema, D.J., B.J. BootsMiller, T.E. Vaughn, R.F. Woolson, et al.. Antimicrobial resistance trends and outbreak frequency in United States hospitals. Clin Infect Dis, 2004. 38: p. 78-85. 10. Hsueh, P.R., L.J. Teng, W.H. Chen, H.J. Pan, et al.. Increasing prevalence of methicillin-resistant Staphylococcus aureus causing nosocomial infections at a university hospital in Taiwan from 1986 to 2001. Antimicrob Agents Chemother, 2004. 48: p. 1361-4. 11. Hanssen, A.M. and J.U. Ericson Sollid. SCCmec in staphylococci: genes on the move. FEMS Immunol Med Microbiol, 2006. 46: p. 8-20. 12. Teruyo Ito. Classification of staphylococcal cassette chromosome mec (SCCmec): guidelines for reporting novel SCCmec elements. Antimicrob Agents Chemother, 2009. 53: p. 4961-7. 13. Chambers, H.F. and F.R. Deleo. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol, 2009. 7: p. 629-41. 14. Monds, R.D. and G.A. O'Toole. The developmental model of microbial biofilms: ten years of a paradigm up for review. Trends Microbiol, 2009. 17: p. 73-87. 15. Huseby, M.J., A.C. Kruse, J. Digre, P.L. Kohler, et al.. Beta toxin catalyzes formation of nucleoprotein matrix in staphylococcal biofilms. Proc Natl Acad Sci U S A, 2010. 107: p. 14407-12. 16. Stoodley, P., K. Sauer, D.G. Davies and J.W. Costerton. Biofilms as complex differentiated communities. Annu Rev Microbiol, 2002. 56: p. 187-209. 17. Stanley, N.R. and B.A. Lazazzera. Environmental signals and regulatory pathways that influence biofilm formation. Mol Microbiol, 2004. 52: p. 917-24. 18. Kong, K.F., C. Vuong and M. Otto. Staphylococcus quorum sensing in biofilm formation and infection. Int J Med Microbiol, 2006. 296: p. 133-9. 19. Boles, B.R. and A.R. Horswill. agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog, 2008. 4: p. e1000052. 20. Lewis, K.. Persister cells, dormancy and infectious disease. Nat Rev Microbiol, 2007. 5: p. 48-56. 21. Wolcott, R.D. and G.D. Ehrlich. Biofilms and chronic infections. JAMA, 2008. 299: p. 2682-4. 22. Kostenko, V., M.M. Salek, P.Sattari and R.J. Martinuzzi. Staphylococcus aureus biofilm formation and tolerance to antibiotics in response to oscillatory shear stresses of physiological levels. FEMS Immunol Med Microbiol, 2010. 59: p. 421-31. 23. Ceri, H., M.E. Olson, C. Stremick, R.R. Read, et al.. The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol, 1999. 37: p. 1771-6. 24. Chuard, C., P. Vaudaux, F.A. Waldvogel and D.P. Lew. Susceptibility of Staphylococcus aureus growing on fibronectin-coated surfaces to bactericidal antibiotics. Antimicrob Agents Chemother, 1993. 37: p. 625-32. 25. Donlan, R.M.. Role of biofilms in antimicrobial resistance. ASAIO J, 2000. 46: p. S47-52. 26. Center of laser medicine. Historical aspects of photodynamic therapy development. http://www.magicray.ru/ENG/lecture/L2/2.html 27. Maisch, T.. Anti-microbial photodynamic therapy: useful in the future? Lasers Med Sci, 2007. 22: p. 83-91. 28. Wainwright, M.. Photodynamic antimicrobial chemotherapy (PACT). J Antimicrob Chemother, 1998. 42: p. 13-28. 29. Wildeman, M.A., H.J. Nyst, B. Karakullukcu and B.I. Tan. Photodynamic therapy in the therapy for recurrent/persistent nasopharyngeal cancer. Head Neck Oncol, 2009. 1: p. 40. 30. Dougherty, T.J., J.E. Kaufman, A. Goldfarb, K.R. Weishaupt, et al.. Photoradiation therapy for the treatment of malignant tumors. Cancer Res, 1978. 38: p. 2628-35. 31. Dimofte, A., T.C. Zhu, S.M. Hahn and R.A. Lustig. In vivo light dosimetry for motexafin lutetium-mediated PDT of recurrent breast cancer. Lasers Surg Med, 2002. 31: p. 305-12. 32. Balchum, O.J., D.R. Doiron and G.C. Huth, Photoradiation therapy of endobronchial lung cancers employing the photodynamic action of hematoporphyrin derivative. Lasers Surg Med, 1984. 4: p. 13-30. 33. Jori, G., C. Fabris, M. Soncin, S. Ferro, et al.., Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Lasers Surg Med, 2006. 38: p. 468-81. 34. Hamblin, M.R., D.A. O’Donnell, N. Murthy, C.H. Contag, et al.. Rapid control of wound infections by targeted photodynamic therapy monitored by in vivo bioluminescence imaging. Photochem Photobiol, 2002. 75: p. 51-7. 35. Hamblin, M.R. and T. Hasan. Photodynamic therapy: a new antimicrobial approach to infectious disease? Photochem Photobiol Sci, 2004. 3: p. 436-50. 36. Suzuki, K., T. Mikami, Y. Okawa, A. Tokoro, et al.. Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose. Carbohydr Res, 1986. 151: p. 403-8. 37. Peniche , C., W. Arguelles-Monal, H. Peniche and N. Acosta. Chitosan: an attractive biocompatible polymer for microencapsulation, 2003. 3: p. 511-20 38. Je, J.Y. and S.K. Kim. Chitosan derivatives killed bacteria by disrupting the outer and inner membrane. J Agric Food Chem, 2006. 54: p. 6629-33. 39. Aranaz, I, M. Mengibar, R. Harris, I. Panos, et al.. Functional Characterization of Chitin and Chitosan, 2009. 3: p. 203-30. 40. Lawrence, R.C., T.F. Fryer and B. Reiter. Rapid method for the quantitative estimation of microbial lipases. Nature, 1967. 213: p. 1264-65. 41. Burger, M., R.G. Woods, C. McCarthy and I.R. Beacham. Temperature regulation of protease in Pseudomonas fluorescens LS107d2 by an ECF sigma factor and a transmembrane activator. Microbiology, 2000. 146: p. 3149-55. 42. Wong, T.W., Y.Y. Wang, H.M. Sheu and Y.C. Chuang. Bactericidal effects of toluidine blue-mediated photodynamic action on Vibrio vulnificus. Antimicrob Agents Chemother, 2005. 49: p. 895-902. 43. Tsai, T., H.F. Chien, T.H. Wang, C.T. Huang, et al.. Chitosan augments photodynamic inactivation of gram-positive and gram-negative bacteria. Antimicrob Agents Chemother, 2011. 55: p. 1883-90. 44. Saginur, R., M. Stdenis, W. Ferris, S.D. Aaron, et al.. Multiple combination bactericidal testing of staphylococcal biofilms from implant-associated infections. Antimicrob Agents Chemother, 2006. 50: p. 55-61. 45. Soukos, N.S., M. Wilson, T. Burns and P.M. Speight. Photodynamic effects of toluidine blue on human oral keratinocytes and fibroblasts and Streptococcus sanguis evaluated in vitro. Lasers Surg Med, 1996. 18: p. 253-9. 46. Merchat, M., G. Bertolini, P. Giacomini, A. Villanueva, et al.. Meso-substituted cationic porphyrins as efficient photosensitizers of gram-positive and gram-negative bacteria. J Photochem Photobiol B, 1996. 32: p. 153-7. 47. Merchat, M., J.D. Spikes, G. Bertoloni and G. Jori. Studies on the mechanism of bacteria photosensitization by meso-substituted cationic porphyrins. J Photochem Photobiol B, 1996. 35: p. 149-57. 48. Minnock, A., D.I. Vernon, J. Schofield, J. Griffiths, et al.. Photoinactivation of bacteria. Use of a cationic water-soluble zinc phthalocyanine to photoinactivate both gram-negative and gram-positive bacteria. J Photochem Photobiol B, 1996. 32: p. 159-64. 49. Kong, M., X.G. Chen, K. Xing and H.J. Park. Antimicrobial properties of chitosan and mode of action: A state of the art review. Int J Food Microbiol, 2010. 144: p. 51-63. 50. Nagpal, K., S.K. Singh and D.N. Mishra. Chitosan nanoparticles: a promising system in novel drug delivery. Chem Pharm Bull (Tokyo), 2010. 58: p. 1423-30. 51. Chung, Y.C., Y.P. Su, C.C. Chen, G. Jia, et al. Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. Acta Pharmacol Sin, 2004. 25: p. 932-6. 52. Zhong, Z., R. Xing, S. Liu, L. Wang, et al. Synthesis of acyl thiourea derivatives of chitosan and their antimicrobial activities in vitro. Carbohydr Res, 2007. 343: p. 566-70. 53. Costerton, J.W., Z. Lewandowski, D.E. Caldwell, D.R. Korber, et al.. Microbial biofilms. Annu Rev Microbiol, 1995. 49: p. 711-45. 54. Coenye, T. and H.J. Nelis. In vitro and in vivo model systems to study microbial biofilm formation. J Microbiol Methods, 2010. 83: p. 89-105. 55. Ali, L., F. Khambaty and G. Diachenko. Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents. Bioresour Technol, 2006. 97: p. 1887-93. 56. Niu, C. and E.S. Gilbert. Colorimetric method for identifying plant essential oil components that affect biofilm formation and structure. Appl Environ Microbiol, 2004. 70: p. 6951-6. 57. Merritt, J. H., D. E. Kadouri and G. A. O’Toole. 2005. Growing and analyzing static biofilms. Current Protocols in Microbiology Chapter 1: Unit 1B. 1. 58. Pitts, B., A. Willse, G.A. McFeters, M.A. Hamilton, et al.. A repeatable laboratory method for testing the efficacy of biocides against toilet bowl biofilms. J Appl Microbiol, 2001. 91: p. 110-7. 59. CASEIN PRODUCTS. http://nzic.org.nz/ChemProcesses/dairy/3E.pdf 60. Sudarshan, N.R., D.G. Hoover and D. Knorr. Antibacterial action of chitosan. Food Biotechnology, 1992. 6: p. 257-72. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37684 | - |
dc.description.abstract | 隨著抗生素的濫用,許多細菌對抗生素產生了抗藥性,例如methicillin-
resistant Staphylococcus aureus (MRSA)的出現。隨著抗藥菌株的增加及多重抗藥菌株的出現,顯示出除了傳統的抗生素治療外,需要開發另一項抗菌方式來治療細菌的感染,例如光動力殺菌。光動力殺菌的原理是透過特定波長之光源將特定的光感物質加以激發,藉由電子之間的能量轉移產生自由基來毒殺細菌,或是藉由能量轉移至基態的氧分子形成激發單態的單態氧(singlet oxygen),攻擊細菌的細胞壁、細胞膜、核酸等結構。甲殼素(chitosan)為幾丁質(chitin)經處理及去乙醯化後所得,具有抑制細菌的特性,本實驗將探討甲殼素是否有協同光動力殺菌的作用。 由於世界各地的MRSA感染的比例有增加的趨勢,顯然在MRSA的治療方面有愈來愈大的挑戰性。微生物形成的生物膜對生物抑制劑的抗性比懸浮菌體高出100~1000倍以上,生物膜的防治在臨床上為一棘手的問題。本實驗以Staphylococcus aureus為對象,一株為ATCC 29213 (methicillin-sensitive S. aureus)、另一株為ATCC 33592 (MRSA),其他還挑選四株臨床的MRSA菌株。在光感物質方面所選用的為帶正電的Toluidine Blue O (TBO),利用紅色LED光源將此激發,探討TBO與甲殼素對於懸浮菌體與生物膜兩種狀態下的協同光動力殺菌成效。此外,也探究甲殼素協同TBO光動力殺菌對於S. aureus的protease活性之影響 。 經由實驗結果顯示,S. aureus懸浮菌體在TBO低濃度情況下,甲殼素已明顯有協同光動力殺菌作用;然而,生物膜菌體要在TBO較高濃度時甲殼素才會有協同殺菌效果。光動力作用對於生物膜的影響比懸浮菌體小,可能是生物膜的基因表現與特性有所不同所導致。未抗藥、抗藥菌株與不同SCCmec type之間對於協同光動力殺菌作用的感受性沒有明顯的差異,都具有甲殼素協同TBO光動力殺菌效果。甲殼素也具有抑制protease活性之成效。 | zh_TW |
dc.description.abstract | Like the appearance of methicillin-resistant Staphylococcus aureus (MRSA), the developments of antibiotics-resistant events among a variety of bacteria are due to antibiotics abuses. Drug-resistant strains spring up like mushrooms that indicates antibiotics therapy not enough. To solve these problems, photodynamic inactivation is other anti-microbial strategies. Photodynamic inactivation employs visible light of appropriate wavelength to excite the photosensitizer and then the photosensitizer goes through a transition from ground state to triplet excited state. The triplet excited photosensitizer generates free radicals from electron transfer or reacts with oxygen molecules to produce singlet oxygen. Free radicals and singlet oxygen are toxic to bacteria because of destroying their components such as cell wall, cell membrane and nucleotides. Chitosan is from chitin undergoing deacetylation, and it exhibits antimicrobial effects. In this study, we investigated whether TBO combined with chitosan showed synergistic photodynamic inactivation.
The trends of increasing prevalence of MRSA infections worldwide indicate that there are more challenges of MRSA therapy. Biofilms are 100 to 1,000 times less susceptible to antibiotics than planktonic bacteria, so it is difficult to handle biofilm prevention. This study focused on Staphylococcus aureus; one was ATCC 29213 (methicillin-sensitive S. aureus), another was ATCC 33592 (MRSA), and the others were four clinical MRSA strains. The photosensitizer was chosen cationic toluidine blue O (TBO) and then we used red LED light to excite it for evaluating the synergistic photodynamic inactivation efficiency of TBO combined with chitosan against planktonic and biofilm cells. In addition, this study also explored the influence of protease activity under TBO combined with chitosan treatment. The synergistic photodynamic inactivation for S. aureus planktonic cells could be detected under the conditions of low concentration of TBO; however, the effect for biofilm cells was only observed when TBO doses were increased. The synergistic photodynamic inactivation that was supposed to be associated with various genetic regulation and characteristics of bacterial stages affected biofilms less than planktonic cells. According to the results, there was no obvious difference of the synergistic photodynamic inactivation efficiency between methicillin-susceptible or resistant strains including diverse SCCmec types. Thus, the synergistic photodynamic activity by TBO combined with chitosan was shown in all of the tested S. aureus strains. Moreover, chitosan had the ability to inhibit protease activities of S. aureus. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:38:33Z (GMT). No. of bitstreams: 1 ntu-100-R98424002-1.pdf: 1203168 bytes, checksum: b75534be4e4e48ada3417dca2f774a0c (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 目錄
致謝.............................................................i 中文摘要.............................................................ii Abstract............................................................iii 目錄.................................................................v 圖目錄..............................................................vii 第一章、 緒論........................................................1 第一節、 MRSA....................................................1 第二節、 生物膜...................................................2 第三節、 光動力治療................................................3 第四節、 生醫材料Chitosan........................................5 第二章、 研究動機與實驗設計..........................................6 第三章、 材料與方法..................................................7 【第一部分】........................................................7 【第二部分】.......................................................13 【第三部分】.......................................................15 第四章、 實驗結果...................................................17 【第一部分】.......................................................17 【第二部分】.......................................................23 【第三部分】.......................................................26 第五章、 討論.......................................................28 【第一部分】.......................................................28 【第二部分】.......................................................30 【第三部分】.......................................................33 第六章、 實驗結果之附圖.............................................35 第七章、 附錄.......................................................65 第八章、 參考文獻...................................................66 | |
dc.language.iso | zh-TW | |
dc.title | 甲苯胺藍與甲殼素協同光動力殺菌對於金黃色葡萄球菌之懸浮菌體及生物膜之研究 | zh_TW |
dc.title | Synergistic Photodynamic Inactivation of Toluidine Blue O and Chitosan on Staphylococcus aureus Planktonic and Biofilm Cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖淑貞,俞松良,陳進庭 | |
dc.subject.keyword | 金黃色葡萄球菌,甲苯胺藍,甲殼素,光動力殺菌, | zh_TW |
dc.subject.keyword | Staphylococcus aureus,toluidine blue O,chitosan,photodynamic inactivation, | en |
dc.relation.page | 70 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-10 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 1.17 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。