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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張明富(Ming-Fu Chang) | |
| dc.contributor.author | Pei-Yin Shih | en |
| dc.contributor.author | 施佩吟 | zh_TW |
| dc.date.accessioned | 2021-06-13T15:34:43Z | - |
| dc.date.available | 2011-08-13 | |
| dc.date.copyright | 2008-08-13 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-07-11 | |
| dc.identifier.citation | 1. Rizzetto M, Canese MG, Arico S, Crivelli O, Trepo C, Bonino F, Verme G. Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers. Gut 1977,18:997-1003.
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J Virol 1993,67:2529-2536. 54. Chang MF, Chen CJ, Chang SC. Mutational analysis of delta antigen: effect on assembly and replication of hepatitis delta virus. J Virol 1994,68:646-653. 55. Chang MF, Chang SC, Chang CI, Wu K, Kang HY. Nuclear localization signals, but not putative leucine zipper motifs, are essential for nuclear transport of hepatitis delta antigen. J Virol 1992,66:6019-6027. 56. Wang JG, Lemon SM. Hepatitis delta virus antigen forms dimers and multimeric complexes in vivo. J Virol 1993,67:446-454. 57. Xia YP, Lai MM. Oligomerization of hepatitis delta antigen is required for both the trans-activating and trans-dominant inhibitory activities of the delta antigen. J Virol 1992,66:6641-6648. 58. Wang CC, Chang TC, Lin CW, Tsui HL, Chu PB, Chen BS, et al. Nucleic acid binding properties of the nucleic acid chaperone domain of hepatitis delta antigen. Nucleic Acids Res 2003,31:6481-6492. 59. Lee CH, Chang SC, Chen CJ, Chang MF. The nucleolin binding activity of hepatitis delta antigen is associated with nucleolus targeting. J Biol Chem 1998,273:7650-7656. 60. Ghisolfi L, Joseph G, Amalric F, Erard M. The glycine-rich domain of nucleolin has an unusual supersecondary structure responsible for its RNA-helix-destabilizing properties. J Biol Chem 1992,267:2955-2959. 61. Tuteja N, Huang NW, Skopac D, Tuteja R, Hrvatic S, Zhang J, et al. Human DNA helicase IV is nucleolin, an RNA helicase modulated by phosphorylation. Gene 1995,160:143-148. 62. Lee CZ, Lin JH, Chao M, McKnight K, Lai MM. RNA-binding activity of hepatitis delta antigen involves two arginine-rich motifs and is required for hepatitis delta virus RNA replication. J Virol 1993,67:2221-2227. 63. Poisson F, Roingeard P, Baillou A, Dubois F, Bonelli F, Calogero RA, Goudeau A. Characterization of RNA-binding domains of hepatitis delta antigen. J Gen Virol 1993,74 (Pt 11):2473-2478. 64. Chao M, Hsieh SY, Taylor J. The antigen of hepatitis delta virus: examination of in vitro RNA-binding specificity. J Virol 1991,65:4057-4062. 65. Lin JH, Chang MF, Baker SC, Govindarajan S, Lai MM. Characterization of hepatitis delta antigen: specific binding to hepatitis delta virus RNA. J Virol 1990,64:4051-4058. 66. Lee CH, Chang SC, Wu CH, Chang MF. A novel chromosome region maintenance 1-independent nuclear export signal of the large form of hepatitis delta antigen that is required for the viral assembly. J Biol Chem 2001,276:8142-8148. 67. Wang YH, Chang SC, Huang C, Li YP, Lee CH, Chang MF. Novel nuclear export signal-interacting protein, NESI, critical for the assembly of hepatitis delta virus. J Virol 2005,79:8113-8120. 68. Glenn JS, Watson JA, Havel CM, White JM. Identification of a prenylation site in delta virus large antigen. Science 1992,256:1331-1333. 69. Moores SL, Schaber MD, Mosser SD, Rands E, O'Hara MB, Garsky VM, et al. Sequence dependence of protein isoprenylation. J Biol Chem 1991,266:14603-14610. 70. Huang C, Chang SC, Yu IC, Tsay YG, Chang MF. Large hepatitis delta antigen is a novel clathrin adaptor-like protein. J Virol 2007,81:5985-5994. 71. Chang FL, Chen PJ, Tu SJ, Wang CJ, Chen DS. The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus. Proc Natl Acad Sci USA 1991,88:8490-8494. 72. Chen PJ, Chang FL, Wang CJ, Lin CJ, Sung SY, Chen DS. Functional study of hepatitis delta virus large antigen in packaging and replication inhibition: role of the amino-terminal leucine zipper. J Virol 1992,66:2853-2859. 73. Macnaughton TB, Gowans EJ, Jilbert AR, Burrell CJ. Hepatitis delta virus RNA, protein synthesis and associated cytotoxicity in a stably transfected cell line. Virology 1990,177:692-698. 74. Macnaughton TB, Gowans EJ, Reinboth B, Jilbert AR, Burrell CJ. Stable expression of hepatitis delta virus antigen in a eukaryotic cell line. J Gen Virol 1990,71 ( Pt 6):1339-1345. 75. Hwang SB, Park KJ, Kim YS. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37607 | - |
| dc.description.abstract | D 型肝炎病毒（hepatitis delta virus, HDV）是一種小型球狀RNA 病毒，內部由單股RNA 基因體以及兩種delta 抗原所構成，外套膜則由B 型肝炎表面抗原（hepatitis B virus surface antigen, HBsAg）及脂雙層（lipid bilayer）形成。雖然已知HDV 在HBsAg 存在下具有感染力，但是目前對於HDV 造成肝炎發生的機制目前仍未完全瞭解。
HDV 內部的兩種delta 抗原是其基因體轉譯產生的唯一蛋白質，分別為對於病毒顆粒包裹所必須的大型delta（HDAg-L）以及複製所必須的小型delta（HDAg-S）抗原。先前的研究利用報導基因表現分析（reporter gene assay）發現大型delta 抗原可以活化血清反應因子（serum response factor, SRF）調控的基因轉錄以及轉形生長因子β（transforming growth factor beta, TGF-β）訊息傳遞路徑。為了進一步瞭解delta 抗原在基因表現調控所扮演的角色，我們首先利用染色質免疫沉澱技術（chromatin immunoprecipitation, ChIP）證實大型及小型delta 抗原皆會坐落在宿主細胞血漿纖溶酶原活化劑抑制物-1（plasminogen activator inhibitor 1, PAI-1）基因啟動子上。接著以免疫沉澱（immunoprecipitation, IP）及質譜分析，進一步探討delta 抗原與宿主細胞中參與轉錄因子之結合發現，可能與delta 抗原有結合的蛋白質中有5 個與轉錄調控有關。以免疫共沉澱證實，與細胞生長分化相關的TAp63γ確實和delta 抗原有專一性結合。另外，我們同時也發現在穩定表現大型delta 抗原的HepG2 細胞中，TAp63γ 的mRNA 及蛋白質表現量皆有增加的情形。到底delta抗原與p63 的結合和轉錄調控對宿主細胞的影響亦進行討論。 | zh_TW |
| dc.description.abstract | Hepatitis delta virus (HDV) is a spherical enveloped virus. The viral genome consists of a single-stranded, negative sense, circular RNA of 1.7 kb that encodes the only known HDV protein, hepatitis delta antigen (HDAg). There are two forms of delta antigen, a small delta antigen (HDAg-S, 195 amino acids, 24 kDa) and a large delta antigen (HDAg-L, 214 amino acids, 27 kDa). HDAg-S is essential for the viral genome replication, whereas HDAg-L is required for the viral assembly.
HDV is a satellite virus that requires hepatitis B virus as its helper virus to provide surface antigen (HBsAg) for natural transmission. Although it is known that HDV infection increases liver disease severity, the underlying mechanisms are poorly understood. Previous studies have demonstrated that HDAg-L could activate serum response factor-associated transcription and TGF-β signaling pathway. To further understand the role of HDAg in the expression of host genes, chromatin immunoprecipitation assay was conducted in this study. The results emonstrated that both HDAg-L and HDAg-S bound to the promoter region of plasminogen activator inhibitor (PAI-1) gene. In addition, immunoprecipitation followed by LC-MS/MS analysis were performed to identify other cellular factors that interact with HDAg. Results showed that p63, a member of p53 family, known to be involved in the transcription regulation of cell growth and differentiation was identified to be associated with HDAg. Further studies demonstrated that TAp63γ, an isoform of p63, interacted with both HDAg-L and HDAg-S. Moreover, the expression levels of TAp63γ mRNA and protein were remarkedly increased in cells stably expressing HDAg-L. How HDAg regulates expression of host genes through p63 remains to be elucidated. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T15:34:43Z (GMT). No. of bitstreams: 1 ntu-97-R95442006-1.pdf: 897224 bytes, checksum: 8b7e99118d4d5d217fedd18cd42e5d78 (MD5) Previous issue date: 2008 | en |
| dc.description.tableofcontents | 中文摘要........................................................................... i
英文摘要........................................................................... ii 縮寫表............................................................................... iv 緒論................................................................................... 1 實驗材料來源.................................................................. 13 實驗方法.......................................................................... 16 實驗結果.......................................................................... 27 討論.................................................................................. 31 圖表.................................................................................. 36 參考文獻.......................................................................... 52 | |
| dc.language.iso | zh-TW | |
| dc.subject | 轉錄調控 | zh_TW |
| dc.subject | D型肝炎抗原 | zh_TW |
| dc.subject | gene regulation | en |
| dc.subject | hepatitis delta antigen | en |
| dc.subject | TAp63 | en |
| dc.title | D型肝炎抗原調控轉錄因子TAp63之基因表現 | zh_TW |
| dc.title | Hepatitis delta antigen regulates the expression of transcription factor TAp63 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張智芬,羅?升 | |
| dc.subject.keyword | D型肝炎抗原,轉錄調控, | zh_TW |
| dc.subject.keyword | hepatitis delta antigen,TAp63,gene regulation, | en |
| dc.relation.page | 60 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-07-11 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 生物化學暨分子生物學研究所 | zh_TW |
| 顯示於系所單位： | 生物化學暨分子生物學科研究所 | |
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