請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37560
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 莊榮輝(Rong-Huay Juang) | |
dc.contributor.author | Chih-Yu Shen | en |
dc.contributor.author | 沈志昱 | zh_TW |
dc.date.accessioned | 2021-06-13T15:32:44Z | - |
dc.date.available | 2008-07-21 | |
dc.date.copyright | 2008-07-21 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-14 | |
dc.identifier.citation | Andersen, K.M., Semple, C.A., and Hartmann-Petersen, R. (2007). Characterisation of the nascent polypeptide-associated complex in fission yeast. Mol Biol Rep 34, 275-281.
Aoki, F., Worrad, D.M., and Schultz, R.M. (1997). Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo. Dev Biol 181, 296-307. Betts, D.H., and King, W.A. (2001). Genetic regulation of embryo death and senescence. Theriogenology 55, 171-191. Braude, P., Bolton, V., and Moore, S. (1988). Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature 332, 459-461. Camous, S., Kopecny, V., and Flechon, J.E. (1986). Autoradiographic detection of the earliest stage of [3H]-uridine incorporation into the cow embryo. Biol Cell 58, 195-200. Cartegni, L., Chew, S.L., and Krainer, A.R. (2002). Listening to silence and understanding nonsense: exonic mutations that affect splicing. Nat Rev Genet 3, 285-298. Crosby, I.M., Gandolfi, F., and Moor, R.M. (1988). Control of protein synthesis during early cleavage of sheep embryos. J Reprod Fertil 82, 769-775. Dihazi, H., Muller, G.A., Lindner, S., Meyer, M., Asif, A.R., Oellerich, M., and Strutz, F. (2007). Characterization of diabetic nephropathy by urinary proteomic analysis: identification of a processed ubiquitin form as a differentially excreted protein in diabetic nephropathy patients. Clin Chem 53, 1636-1645. Ducibella, T., Huneau, D., Angelichio, E., Xu, Z., Schultz, R.M., Kopf, G.S., Fissore, R., Madoux, S., and Ozil, J.P. (2002). Egg-to-embryo transition is driven by differential responses to Ca2+ oscillation number. Dev Biol 250, 280-291. Funfschilling, U., and Rospert, S. (1999). Nascent polypeptide-associated complex stimulates protein import into yeast mitochondria. Mol Biol Cell 10, 3289-3299. Gardner, R.L. (1997). The early blastocyst is bilaterally symmetrical and its axis of symmetry is aligned with the animal-vegetal axis of the zygote in the mouse. Development 124, 289-301. Gygi, S.P., Rochon, Y., Franza, B.R., and Aebersold, R. (1999). Correlation between protein and mRNA abundance in yeast. Mol Cell Biol 19, 1720-1730. Hamatani, T., Carter, M.G., Sharov, A.A., and Ko, M.S. (2004). Dynamics of global gene expression changes during mouse preimplantation development. Dev Cell 6, 117-131. Jarrell, V.L., Day, B.N., and Prather, R.S. (1991). The transition from maternal to zygotic control of development occurs during the 4-cell stage in the domestic pig, Sus scrofa: quantitative and qualitative aspects of protein synthesis. Biol Reprod 44, 62-68. Kikyo, N., and Wolffe, A.P. (2000). Reprogramming nuclei: insights from cloning, nuclear transfer and heterokaryons. J Cell Sci 113 ( Pt 1), 11-20. Latham, K.E., Garrels, J.I., Chang, C., and Solter, D. (1991). Quantitative analysis of protein synthesis in mouse embryos. I. Extensive reprogramming at the one- and two-cell stages. Development 112, 921-932. Lauring, B., Sakai, H., Kreibich, G., and Wiedmann, M. (1995). Nascent polypeptide-associated complex protein prevents mistargeting of nascent chains to the endoplasmic reticulum. Proc Natl Acad Sci U S A 92, 5411-5415. MacBeath, G. (2001). Proteomics comes to the surface. Nat Biotechnol 19, 828-829. Manes, C. (1973). The participation of the embryonic genome during early cleavage in the rabbit. Dev Biol 32, 453-459. Maro, B., Johnson, M.H., Pickering, S.J., and Flach, G. (1984). Changes in actin distribution during fertilization of the mouse egg. J Embryol Exp Morphol 81, 211-237. Meirelles, F.V., Caetano, A.R., Watanabe, Y.F., Ripamonte, P., Carambula, S.F., Merighe, G.K., and Garcia, S.M. (2004). Genome activation and developmental block in bovine embryos. Anim Reprod Sci 82-83, 13-20. Memili, E., and First, N.L. (2000). Zygotic and embryonic gene expression in cow: a review of timing and mechanisms of early gene expression as compared with other species. Zygote 8, 87-96. Prioleau, M.N., Huet, J., Sentenac, A., and Mechali, M. (1994). Competition between chromatin and transcription complex assembly regulates gene expression during early development. Cell 77, 439-449. Redman, K.L., and Burris, G.W. (1996). The cDNA for the ubiquitin-52-amino-acid fusion protein from rat encodes a previously unidentified 60 S ribosomal subunit protein. Biochem J 315 ( Pt 1), 315-321. Rospert, S., Dubaquie, Y., and Gautschi, M. (2002). Nascent-polypeptide-associated complex. Cell Mol Life Sci 59, 1632-1639. Ruzov, A., Dunican, D.S., Prokhortchouk, A., Pennings, S., Stancheva, I., Prokhortchouk, E., and Meehan, R.R. (2004). Kaiso is a genome-wide repressor of transcription that is essential for amphibian development. Development 131, 6185-6194. Sarmento, O.F., Digilio, L.C., Wang, Y., Perlin, J., Herr, J.C., Allis, C.D., and Coonrod, S.A. (2004). Dynamic alterations of specific histone modifications during early murine development. J Cell Sci 117, 4449-4459. Schatten, G., Simerly, C., and Schatten, H. (1985). Microtubule configurations during fertilization, mitosis, and early development in the mouse and the requirement for egg microtubule-mediated motility during mammalian fertilization. Proc Natl Acad Sci U S A 82, 4152-4156. Schier, A.F. (2007). The maternal-zygotic transition: death and birth of RNAs. Science 316, 406-407. Schini, S.A., and Bavister, B.D. (1988). Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol Reprod 39, 1183-1192. Spreter, T., Pech, M., and Beatrix, B. (2005). The crystal structure of archaeal nascent polypeptide-associated complex (NAC) reveals a unique fold and the presence of a ubiquitin-associated domain. J Biol Chem 280, 15849-15854. Tay, T.L., Lin, Q., Seow, T.K., Tan, K.H., Hew, C.L., and Gong, Z. (2006). Proteomic analysis of protein profiles during early development of the zebrafish, Danio rerio. Proteomics 6, 3176-3188. Wright, P.W., Bolling, L.C., Calvert, M.E., Sarmento, O.F., Berkeley, E.V., Shea, M.C., Hao, Z., Jayes, F.C., Bush, L.A., Shetty, J., Shore, A.N., Reddi, P.P., Tung, K.S., Samy, E., Allietta, M.M., Sherman, N.E., Herr, J.C., and Coonrod, S.A. (2003). ePAD, an oocyte and early embryo-abundant peptidylarginine deiminase-like protein that localizes to egg cytoplasmic sheets. Dev Biol 256, 73-88. Yotov, W.V., Moreau, A., and St-Arnaud, R. (1998). The alpha chain of the nascent polypeptide-associated complex functions as a transcriptional coactivator. Mol Cell Biol 18, 1303-1311. Zernicka-Goetz, M. (2005). Cleavage pattern and emerging asymmetry of the mouse embryo. Nat Rev Mol Cell Biol 6, 919-928. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37560 | - |
dc.description.abstract | 在哺乳類動物早期胚胎發育過程中,有數個重要的轉變時期,其中由母源基因轉換至胚源基因的過程,是影響胚胎能否正常發育至囊胚期的第一個重要關卡。過去的研究多半是利用微陣列去分析轉換前後胚胎的所有基因表現,而鮮少有蛋白質層次的研究成果。本論文自豬胚胎 cDNA 資料庫中,鎖定三種共同存在於小鼠與人類胚胎,且在母源-胚源轉換過程表現量最顯著的蛋白質,嘗試以合成短鏈胜 (月太) 免疫誘發出的抗體,有些能夠辨認合成的胜 (月太) 片段,但均無法辨認胚胎上的目標蛋白質。來自豬胚胎的三個未知身份蛋白質,均已製備出單株化抗體,其中兩個完成腹水大量製備,但以小鼠胚胎測試均無效價,豬胚胎測試則因樣本數不足而尚未進行。另一方面,在二次元電泳膠片上,挖取小鼠二細胞與四細胞期胚胎 19 個蛋白質色點作質譜儀身份鑑定後,對於小鼠胚胎母源-胚源轉換過程的生理反應,已有蛋白質層次的初步整體概念。 | zh_TW |
dc.description.abstract | Among the transition stages during mammalian preimplantation development, maternal to zygotic transition (MZT) is the first key check point for embryo to maintain normal cleavage through blastocyst stage. Few researchers addressed the difference of protein expression between these transitional stages; only microarray analysis for the gene expression has been reported if any. In this study, three target proteins expressed remarkably during MZT in mouse and human embryos are used as the antigen (synthetic peptides) for antibody preparation. In addition, three unknown genes from porcine embryos were expressed and also used for antibody preparation. Finally, by using proteomic approaches, the protein patterns between mouse 2-cell and 4-cell embryos were compared. Some of the antibodies induced by synthetic polypeptides were able to binding their target pepitdes. However, none could recognize the original embryonic target protein. On the other hand, we produced monoclonal antibodies against the unknown porcine proteins, and two of them were used to produce ascites. However, these antibodies cannot recognize any protein from mouse embryo. Porcine embryo was not tested. On the two-dimensional elecetrophoresis gel, 19 protein spots reflecting the difference between mouse 2-cell and 4-cell embryo were analyzed by mass spectrometry. The results dipicted the change of the protein profile for the maternal to zygotic transition. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:32:44Z (GMT). No. of bitstreams: 1 ntu-97-R95b47203-1.pdf: 5797325 bytes, checksum: 117348fd59f7af9b5fb55f2cbf208aea (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 中文摘要 i
英文摘要 ii 第一章 緒論 1 1.1 哺乳類動物早期胚胎發育過程概述 1 1.2 早期胚胎發育過程中基因層次的調控 5 1.3 胚胎發育時的發育阻障 8 1.4 母源基因轉換至胚源基因過程的機制 8 1.5 從蛋白質體學角度觀察胚胎發育過程 9 1.6 抗體技術介紹 11 1.7 研究動機與目的 12 第二章 材料與方法 13 2.1 一般分析法 13 2.1.1 蛋白質定量法 13 2.1.2 酵素免疫分析法 14 2.2 一般電泳檢定法 16 2.2.1 SDS 膠體電泳 16 2.2.2 膠體染色法 20 2.2.3 膠片乾燥法及護貝 24 2.3 二維電泳相關實驗 25 2.3.1 二維電泳樣本蛋白質萃取法 25 2.3.2 蛋白質樣本溶解方法 26 2.3.3 二維電泳 26 2.3.4 膠體內蛋白脢水解 29 2.4 蛋白質電泳轉印及免疫染色法 31 2.4.1 蛋白質電泳轉印法 31 2.4.2 酵素免疫染色法 32 2.5 抗體製備相關實驗 34 2.5.1 抗體製備方式 34 2.5.2 抗體純化方式 36 2.5.3 細胞融合 39 2.5.4 細胞保存法 44 2.5.5 單株抗體的生產 45 2.5.6 免疫球蛋白之純化 46 2.6 母鼠超級排卵與取胚胎技術 47 第三章 結果 51 3.1 哺乳類動物胚胎發育過程關鍵蛋白質挑選及短鏈胜 (月太) 合成 51 3.2 免疫產生抗體 56 3.2.1 小鼠方面 56 3.2.2 IgY 方面 57 3.2.3 綜合整理 58 3.2.4 豬胚胎未知身分蛋白質之抗體製備 58 3.3 小鼠胚胎相關的蛋白質體實驗 66 第四章 討論 71 4.1 設計 peptide 免疫小鼠與蛋雞製備抗體 71 4.2 小鼠二細胞與四細胞期胚胎二維電泳蛋白質體學研究 75 第五章 未來方向 79 參考文獻 81 問答錄 85 | |
dc.language.iso | zh-TW | |
dc.title | 哺乳類動物胚胎著床前關鍵蛋白質之抗體製備及蛋白質體研究 | zh_TW |
dc.title | The Antibody Preparation and Proteomic Studies of the Mammalian Preimplantation Embryonic Key Proteins | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳建興(Jiann-Shing Wu),陳翰民(Han-Min Chen),張世宗(Shih-Chung Chang) | |
dc.subject.keyword | 母源-胚源轉換,合成短鏈胜 (月太),抗體製備,蛋白質體學, | zh_TW |
dc.subject.keyword | Maternal to zygotic transition (MZT),Synthetic peptide,Antibody preparation,Proteomics, | en |
dc.relation.page | 91 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-14 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-97-1.pdf 目前未授權公開取用 | 5.66 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。