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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 賈景山 | |
dc.contributor.author | Pei-Min Chen | en |
dc.contributor.author | 陳佩旻 | zh_TW |
dc.date.accessioned | 2021-06-13T15:22:13Z | - |
dc.date.available | 2013-09-11 | |
dc.date.copyright | 2008-09-11 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-23 | |
dc.identifier.citation | Abbe, K., Takahashi, S. & Yamada, T. (1982). Involvement of oxygen-sensitive pyruvate formate-lyase in mixed-acid fermentation by Streptococcus mutans under strictly anaerobic conditions. J Bacteriol 152, 175-182.
Ahmed, S. & Booth, I. R. (1983). The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells. Biochem J 212, 105-112. Ajdic´, D., McShan, W. M., McLaughlin, R. E., Savic´G., Chang J., Carson M. B., Primeaux C., Tian R., Kenton S., Jia H., Lin S., Qian Y., Li S., Zhu H., Najar F., Lai H., White J., Roe B. A. & Ferretti J. J. (2002). Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci USA 99, 14434-14439. Akimaru, K., Utsumi, T., Sato, E. F., Klostergaard, J., Inoue, M. & Utsumi, K. (1992). Role of tyrosyl phosphorylation in neutrophil priming by tumor necrosis factor-alpha and granulocyte colony stimulating factor. Arch Biochem Biophys 298, 703-709. Anderson, D. M., Ebersole, J. L. & Novak, M. J. (1995). Functional properties of nonhuman primate antibody to Porphyromonas gingivalis. Infect Immun 63, 3245-3252. Aoki, H., Shiroza, T., Hayakawa, M., Sato, S. & Kuramitsu, H. K. (1986). Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect Immun 53, 587-594. Aranha, H., Strachan, R. C., Arceneaux, J. E. & Byers, B. R. (1982). Effect of trace metals on growth of Streptococcus mutans in a teflon chemostat. Infect Immun 35, 456-460. Bailey, T. L. & Elkan, C. (1995). The value of prior knowledge in discovering motifs with MEME. Proc Int Conf Intell Syst Mol Biol 3, 21-29. Bakker, E. P. & Harold, F. M. (1980). Energy coupling to potassium transport in Streptococcus faecalis. Interplay of ATP and the protonmotive force. J Biol Chem 255, 433-440. Banas, J. A. & Vickerman, M. M. (2003). Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med 14, 89-99. Bearson, B. L., Wilson, L. & Foster, J. W. (1998). A low pH-inducible, PhoPQ-dependent acid tolerance response protects Salmonella typhimurium against inorganic acid stress. J Bacteriol 180, 2409-2417. Bearson, S., Bearson, B. & Foster, J. W. (1997). Acid stress responses in enterobacteria. FEMS Microbiol Lett 147, 173-180. Belli, W. A. & Marquis, R. E. (1991). Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Appl Environ Microbiol 57, 1134-1138. Bender, G. R., Thibodeau, E. A. & Marquis, R. E. (1985). Reduction of acidurance of streptococcal growth and glycolysis by fluoride and gramicidin. J Dent Res 64, 90-95. Bender, G. R., Sutton, S. V. & Marquis, R. E. (1986). Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci. Infect Immun 53, 331-338. Bergsma, J. & Konings, W. N. (1983). The properties of citrate transport in membrane vesicles from Bacillus subtilis. Eur J Biochem 134, 151-156. Berry, A. M. & Paton, J. C. (1996). Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae. Infect Immun 64, 5255-5262. Beutler, B. A., Milsark, I. W. & Cerami, A. (1985). Cachectin/tumor necrosis factor: production, distribution, and metabolic fate in vivo. J Immunol 135, 3972-3977. Biswas, I., Drake, L., Erkina, D. & Biswas, S. (2008). Involvement of sensor kinases in the stress tolerance response of Streptococcus mutans. J Bacteriol 190, 68-77. Blancato, V. S., Magni, C. & Lolkema, J. S. (2006). Functional characterization and Me2+ ion specificity of a Ca2+-citrate transporter from Enterococcus faecalis. FEBS J 273, 5121-5130. Bokoch, G. M. & Diebold, B. A. (2002). Current molecular models for NADPH oxidase regulation by Rac GTPase. Blood 100, 2692-2696. Boorsma, A., van der Rest, M. E., Lolkema, J. S. & Konings, W. N. (1996). Secondary transporters for citrate and the Mg2+-citrate complex in Bacillus subtilis are homologous proteins. J Bacteriol 178, 6216-6222. Bott, M. (1997). Anaerobic citrate metabolism and its regulation in enterobacteria. Arch Microbiol 167, 78-88. Boyd, D. A., Cvitkovitch, D. G., Bleiweis, A. S., Kiriukhin, M. Y., Debabov, D. V., Neuhaus, F. C. & Hamilton, I. R. (2000). Defects in D-alanyl-lipoteichoic acid synthesis in Streptococcus mutans results in acid sensitivity. J Bacteriol 182, 6055-6065. Brown, S. W. & Sonenshein, A. L. (1996). Autogenous regulation of the Bacillus subtilis glnRA operon. J Bacteriol 178, 2450-2454. Brown, T. A., Jr., Ahn, S. J., Frank, R. N., Chen, Y. Y., Lemos, J. A. & Burne, R. A. (2005). A hypothetical protein of Streptococcus mutans is critical for biofilm formation. Infect Immun 73, 3147-3151. Browngardt, C. M., Wen, Z. T. & Burne, R. A. (2004). RegM is required for optimal fructosyltransferase and glucosyltransferase gene expression in Streptococcus mutans. FEMS Microbiol Lett 240, 75-79. Brynhildsen, L. & Rosswall, T. (1989). Effects of cadmium, copper, magnesium, and zinc on the decomposition of citrate by a Klebsiella sp. Appl Environ Microbiol 55, 1375-1379. Burne, R. A., Chen, Y. Y. & Penders, J. E. (1997). Analysis of gene expression in Streptococcus mutans in biofilms in vitro. Adv Dent Res 11, 100-109. Busch, W. & Saier, M. H., Jr. (2002). The transporter classification (TC) system, 2002. Crit Rev Biochem Mol Biol 37, 287-337. Carlsson, J. (1970). Nutritional requirements of Streptococcus mutans. Caries Res 4, 305-320. Carlsson, J., Kujala, U. & Edlund, M. B. (1985). Pyruvate dehydrogenase activity in Streptococcus mutans. Infect Immun 49, 674-678. Carlsson, J., Iwami, Y. & Yamada, T. (1983). Hydrogen peroxide excretion by oral streptococci and effect of lactoperoxidase-thiocyanate-hydrogen peroxide, Infect Immun 40, 70-80. Castanie-Cornet, M. P., Penfound, T. A., Smith, D., Elliott, J. F. & Foster, J. W. (1999). Control of acid resistance in Escherichia coli. J Bacteriol 181, 3525-3535. Caufield, P. W. (1997). Dental caries--a transmissible and infectious disease revisited: a position paper. Pediatr Dent 19, 491-498. Chan, J., Xing, Y., Magliozzo, R. S. & Bloom, B. R. (1992). Killing of virulent Mycobacterium tuberculosis by reactive nitrogen intermediates produced by activated murine macrophages. J Exp Med 175, 1111-1122. Chia, J. S., Hsu, T. Y., Teng, L. J., Chen, J. Y., Hahn, L. J. & Yang, C. S. (1991). Glucosyltransferase gene polymorphism among Streptococcus mutans strains. Infect Immun 59, 1656-1660. Chia, J. S., Lin, R. H., Lin, S. W., Chen, J. Y. & Yang, C. S. (1993a). Inhibition of glucosyltransferase activities of Streptococcus mutans by a monoclonal antibody to a subsequence peptide. Infect Immun 61, 4689-4695. Chia, J. S., Lin, S. W., Hsu, T. Y., Chen, J. Y., Kwan, H. W. & Yang, C. S. (1993b). Analysis of a DNA polymorphic region in the gtfB and gtfC genes of Streptococcus mutans. Infect Immun 61, 1563-1566. Chia, J. S., Yang, C. S. & Chen, J. Y. (1998). Functional analyses of a conserved region in glucosyltransferases of Streptococcus mutans. Infect Immun 66, 4797-4803. Chia, J. S., Lee, Y. Y., Huang, P. T. & Chen, J. Y. (2001). Identification of stress-responsive genes in Streptococcus mutans by differential display reverse transcription-PCR. Infect Immun 69, 2493-2501. Clarke, J. K. (1924). On the bacterial factor in the aetiology of dental caries. Br. J Exp Pathol 5, 141-146. Claverys, J. P., Dintilhac, A., Pestova, E. V., Martin, B. & Morrison, D. A. (1995). Construction and evaluation of new drug-resistance cassettes for gene disruption mutagenesis in Streptococcus pneumoniae, using an ami test platform. Gene 164, 123-128. Cobine, P., Wickramasinghe, W. A., Harrison, M. D., Weber, T., Solioz, M. & Dameron, C. T. (1999). The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor. FEBS Lett 445, 27-30. Cotter, P. D., O'Reilly, K. & Hill, C. (2001). Role of the glutamate decarboxylase acid resistance system in the survival of Listeria monocytogenes LO28 in low pH foods. J Food Prot 64, 1362-1368. Cotter, P. D. & Hill, C. (2003). Surviving the acid test: responses of gram-positive bacteria to low pH. Microbiol Mol Biol Rev 67, 429-453. Coykendall, A. L. (1989). Classification and identification of the viridans streptococci. Clin Microbiol Rev 2, 315-328. Cvitkovitch, D. G., Boyd, D. A., Thevenot, T. & Hamilton, I. R. (1995). Glucose transport by a mutant of Streptococcus mutans unable to accumulate sugars via the phosphoenolpyruvate phosphotransferase system. J Bacteriol 177, 2251-2258. Cvitkovitch, D. G., Gutierrez, J. A. & Bleiweis, A. S. (1997). Role of the citrate pathway in glutamate biosynthesis by Streptococcus mutans. J Bacteriol 179, 650-655. Dashper, S. G. & Reynolds, E. C. (1992). pH regulation by Streptococcus mutans. J Dent Res 71, 1159-1165. Dashper, S. G., & Reynolds E. C. (1996). Lactic acid excretion by Streptococcus mutans. Microbiol 142, 33-39. Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W. & Greenberg, E. P. (1998). The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280, 295-298. De Jong, M. H. & Van der Hoeven, J. S. (1987). The growth of oral bacteria on saliva. J Dent Res 66, 498-505. de Soet, J. J., Nyvad, B. & Kilian, M. (2000). Strain-related acid production by oral streptococci. Caries Res 34, 486-490. Deng, D. M., Liu, M. J., ten Cate, J. M. & Crielaard, W. (2007). The VicRK system of Streptococcus mutans responds to oxidative stress. J Dent Res 86, 606-610. Ding, A. H., Nathan, C. F. & Stuehr, D. J. (1988). Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol 141, 2407-2412. Dintilhac, A., Alloing, G., Granadel, C. & Claverys, J. P. (1997). Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Mol Microbiol 25, 727-739. Doroshchuk, N. A., M. S. Gelfand, and D. A. Rodionov (2006). Regulation of nitrogen metabolism in gram-positive bacteria. Mol Biol (Mosk) 40, 919-926. Duggal, M. S., Chawla, H. S. & Curzon, M. E. (1991). A study of the relationship between trace elements in saliva and dental caries in children. Arch Oral Biol 36, 881-884. Durack, D. T. & Beeson, P. B. (1972). Experimental bacterial endocarditis. II. Survival of a bacteria in endocardial vegetations. Br J Exp Pathol 53, 50-53. Fabret, C. & Hoch, J. A. (1998). A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. J Bacteriol 180, 6375-6383. Ferreira, A., Sue, D., O'Byrne, C. P. & Boor, K. J. (2003). Role of Listeria monocytogenes sigma(B) in survival of lethal acidic conditions and in the acquired acid tolerance response. Appl Environ Microbiol 69, 2692-2698. Fisher, S. H. (1999). Regulation of nitrogen metabolism in Bacillus subtilis: vive la difference! Mol Microbiol 32, 223-232. Fisher, S. H. & Wray, L. V., Jr. (2006). Feedback-resistant mutations in Bacillus subtilis glutamine synthetase are clustered in the active site. J Bacteriol 188, 5966-5974. Fitzgerald, R. J., Jordan, H. V. & Stanley, H. R. (1960). Experimental caries and gingival pathologic changes in the gnotobiotic rat. J Dent Res 39, 923-935. Fitzgerald, R. J. & Keyes, P. H. (1960). Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc 61, 9-19. Fitzgerald, R. J., Adams, B. O., Sandham, H. J. & Abhyankar, S. (1989). Cariogenicity of a lactate dehydrogenase-deficient mutant of Streptococcus mutans serotype c in gnotobiotic rats. Infect Immun 57, 823-826. Forehand, J. R., Pabst, M. J., Phillips, W. A. & Johnston, R. B., Jr. (1989). Lipopolysaccharide priming of human neutrophils for an enhanced respiratory burst. Role of intracellular free calcium. J Clin Invest 83, 74-83. Fournier, B., Klier, A. & Rapoport, G. (2001). The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureus. Mol Microbiol 41, 247-261. Fozo, E. M. & Quivey, R. G., Jr. (2004). Shifts in the membrane fatty acid profile of Streptococcus mutans enhance survival in acidic environments. Appl Environ Microbiol 70, 929-936. Frees, D., Vogensen, F. K. & Ingmer, H. (2003). Identification of proteins induced at low pH in Lactococcus lactis. Int J Food Microbiol 87, 293-300. Fujiwara, T., Hoshino, T., Ooshima, T. & Hamada, S. (2002). Differential and quantitative analyses of mRNA expression of glucosyltransferases from Streptococcus mutans MT8148. J Dent Res 81, 109-113. Fukushima, K., Motoda, R. & Ikeda, T. (1981). Effects of exogenous insoluble glucan primer on insoluble glucan synthesis by Streptococcus mutans. J Dent Res 60, 1707-1712. Futai, M., Noumi, T. & Maeda, M. (1989). ATP synthase (H+-ATPase): results by combined biochemical and molecular biological approaches. Annu Rev Biochem 58, 111-136. Garcia-Quintans, N., Magni, C., de Mendoza, D. & Lopez, P. (1998). The citrate transport system of Lactococcus lactis subsp. lactis biovar diacetylactis is induced by acid stress. Appl Environ Microbiol 64, 850-857. Gerhardsson, L., Bjorkner, B., Karlsteen, M. & Schutz, A. (2002). Copper allergy from dental copper amalgam? Sci Total Environ 290, 41-46. Gibbons, R. J. & van Houte, J. (1973). On the formation of dental plaques. J Periodontol 44, 347-360. Gibbons, R. J. & Houte, J. V. (1975). Bacterial adherence in oral microbial ecology. Annu Rev Microbiol 29, 19-44. Gomez, A., Ladire, M., Marcille, F. & Fons, M. (2002). Trypsin mediates growth phase-dependent transcriptional regulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota. J Bacteriol 184, 18-28. Goodman, S. D. & Gao, Q. (1999). Firefly luciferase as a reporter to study gene expression in Streptococcus mutans. Plasmid 42, 154-157. Goodman, S. D. & Gao, Q. (2000). Characterization of the gtfB and gtfC promoters from Streptococcus mutans GS-5. Plasmid 43, 85-98. Graf, H. (1970). The glycolytic activity of plaque and its relation to hard tissues pathology--recent findings from intraoral pH telemetry research. Int Dent J 20, 426-435. Griffith, C. J. & Carlsson, J. (1974). Mechanism of ammonia assimilation in streptococci. J Gen Microbiol 82, 253-260. Grisham, M. B. & Ryan, E. M. (1990). Cytotoxic properties of salivary oxidants. Am J Physiol 258, C115-121. Griswold, A. R., Chen, Y. Y. & Burne, R. A. (2004). Analysis of an agmatine deiminase gene cluster in Streptococcus mutans UA159. J Bacteriol 186, 1902-1904. Guthrie, L. A., McPhail, L. C., Henson, P. M. & Johnston, R. B., Jr. (1984). Priming of neutrophils for enhanced release of oxygen metabolites by bacterial lipopolysaccharide. Evidence for increased activity of the superoxide-producing enzyme. J Exp Med 160, 1656-1671. Gutierrez, J. A., Crowley, P. J., Brown, D. P., Hillman, J. D., Youngman, P. & Bleiweis, A. S. (1996). Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements. J Bacteriol 178, 4166-4175. Gutierrez, J. A., Crowley, P. J., Cvitkovitch, D. G., Brady, L. J., Hamilton, I. R., Hillman, J. D. & Bleiweis, A. S. (1999). Streptococcus mutans ffh, a gene encoding a homologue of the 54 kDa subunit of the signal recognition particle, is involved in resistance to acid stress. Microbiology 145, 357-366. Haas, W. & Banas, J. A. (2000). Ligand-binding properties of the carboxyl-terminal repeat domain of Streptococcus mutans glucan-binding protein A. J Bacteriol 182, 728-733. Hahn, K., Faustoferri, R. C. & Quivey, R. G., Jr. (1999). Induction of an AP endonuclease activity in Streptococcus mutans during growth at low pH. Mol Microbiol 31, 1489-1498. Hamada, S. & Slade, H. D. (1980). Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev 44, 331-384. Hamada, S., Torii, M., Kotani, S. & Tsuchitani, Y. (1981). Adherence of Streptococcus sanguinis clinical isolates to smooth surfaces and interactions of the isolates with Streptococcus mutans glucosyltransferase. Infect Immun 32, 364-372. Hamilton, I. R. (1990). Maintenance of proton motive force by Streptococcus mutans and Streptococcus sobrinus during growth in continuous culture. Oral Microbiol Immunol 5, 280-287. Hamilton, I. R. (2000). Ecological basis for dental caries. In Oral Bacterial Ecology: The Molecular Basis. Horizon Scientific Press, Norfolk, U.K., pp. 219-274. Hamilton, I. R. & Buckley, N. D. (1991). Adaptation by Streptococcus mutans to acid tolerance. Oral Microbiol Immunol 6, 65-71. Hamilton, I. R. & Svensater, G. (1998). Acid-regulated proteins induced by Streptococcus mutans and other oral bacteria during acid shock. Oral Microbiol Immunol 13, 292-300. Hanada, N. & Kuramitsu, H. K. (1988). Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infect Immun 56, 1999-2005. Hanada, N. & Kuramitsu, H. K. (1989). Isolation and characterization of the Streptococcus mutans gtfD gene, coding for primer-dependent soluble glucan synthesis. Infect Immun 57, 2079-2085. Hanna, M. N., Ferguson, R. J., Li, Y. H. & Cvitkovitch, D. G. (2001). uvrA is an acid-inducible gene involved in the adaptive response to low pH in Streptococcus mutans. J Bacteriol 183, 5964-5973. Harper, D. S. & Loesche, W. J. (1984). Growth and acid tolerance of human dental plaque bacteria. Arch Oral Biol 29, 843-848. Hartke, A., Bouche, S., Giard, J. C., Benachour, A., Boutibonnes, P. & Auffray, Y. (1996). The lactic acid stress response of Lactococcus lactis subsp. lactis. Curr Microbiol 33, 194-199. Hata, S. & Mayanagi, H. (2003). Acid diffusion through extracellular polysaccharides produced by various mutants of Streptococcus mutans. Arch Oral Biol 48, 431-438. Hava, D. L. & Camilli, A. (2002). Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol Microbiol 45, 1389-1406. Hazlett, K. R., Michalek, S. M. & Banas, J. A. (1998). Inactivation of the gbpA gene of Streptococcus mutans increases virulence and promotes in vivo accumulation of recombinations between the glucosyltransferase B and C genes. Infect Immun 66, 2180-2185. Hendriksen, W. T., Kloosterman, T. G., Bootsma, H. J., Estevao, S., de Groot, R., Kuipers, O. P. & Hermans, P. W. (2008). Site-specific contributions of glutamine-dependent regulator GlnR and GlnR-regulated genes to virulence of Streptococcus pneumoniae. Infect Immun 76, 1230-1238. Higuchi, M. (1992). Reduced nicotinamide adenine dinucleotide oxidase involvement in defense against oxygen toxicity of Streptococcus mutans. Oral Microbiol Immunol 7, 309-314. Higuchi, M., Shimada, M., Yamamoto, Y., Hayashi, T., Koga, T. & Kamio, Y. (1993). Identification of two distinct NADH oxidases corresponding to H2O2-forming oxidase and H2O-forming oxidase induced in Streptococcus mutans. J Gen Microbiol 139, 2343-2351. Higuchi, M., Shimada, M., Matsumoto, J., Yamamoto, Y., Rhaman, A. & Kamio, Y. (1994). Molecular cloning and sequence analysis of the gene encoding the H2O2-forming NADH oxidase from Streptococcus mutans. Biosci Biotechnol Biochem 58, 1603-1607. Higuchi, M., Yamamoto, Y., Poole, L. B., Shimada, M., Sato, Y., Takahashi, N. & Kamio, Y. (1999). Functions of two types of NADH oxidases in energy metabolism and oxidative stress of Streptococcus mutans. J Bacteriol 181, 5940-5947. Higuchi, M., Yamamoto, Y. & Kamio, Y. (2000). Molecular biology of oxygen tolerance in lactic acid bacteria: Functions of NADH oxidases and Dpr in oxidative stress. J Biosci Bioeng 90, 484-493. Hillman, J. D., Chen, A. & Snoep, J. L. (1996). Genetic and physiological analysis of the lethal effect of L-(+)-lactate dehydrogenase deficiency in Streptococcus mutans: complementation by alcohol dehydrogenase from Zymomonas mobilis. Infect Immun 64, 4319-4323. Hillman, J. D., Brooks, T. A., Michalek, S. M., Harmon, C. C., Snoep, J. L. & van Der Weijden, C. C. (2000). Construction and characterization of an effector strain of Streptococcus mutans for replacement therapy of dental caries. Infect Immun 68, 543-549. Hillman, J. D. (2002). Genetically modified Streptococcus mutans for the prevention of dental caries. Antonie van Leeuwenhoek 82, 361-366. Holmes, A. R., McNab, R., Millsap, K. W., Rohde, M., Hammerschmidt, S., Mawdsley, J. L. & Jenkinson, H. F. (2001). The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence. Mol Microbiol 41, 1395-1408. Honda, O., Kato, C. & Kuramitsu, H. K. (1990). Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the glucosyltransferase-S enzyme. J Gen Microbiol 136, 2099-2105. Horaud, T. & Delbos, F. (1984). Viridans streptococci in infective endocarditis: species distribution and susceptibility to antibiotics. Eur Heart J 5, 39-44. Hudson, M. C. & Curtiss, R., 3rd (1990). Regulation of expression of Streptococcus mutans genes important to virulence. Infect Immun 58, 464-470. Idone, V., Brendtro, S., Gillespie, R. & other authors (2003). Effect of an orphan response regulator on Streptococcus mutans sucrose-dependent adherence and cariogenesis. Infect Immun 71, 4351-4360. Iles, K. E. & Forman, H. J. (2002). Macrophage signaling and respiratory burst. Immunol Res 26, 95-105. Jackson, D. W., Simecka, J. W. & Romeo, T. (2002). Catabolite repression of Escherichia coli biofilm formation. J Bacteriol 184, 3406-3410. Jan, G., Leverrier, P., Pichereau, V. & Boyaval, P. (2001). Changes in protein synthesis and morphology during acid adaptation of Propionibacterium freudenreichii. Appl Environ Microbiol 67, 2029-2036. Jansen, W. T., Gootjes, J., Zelle, M., Madore, D. V., Verhoef, J., Snippe, H. & Verheul, A. F. (1998). Use of highly encapsulated Streptococcus pneumoniae strains in a flow-cytometric assay for assessment of the phagocytic capacity of serotype-specific antibodies. Clin Diagn Lab Immunol 5, 703-710. Jayaraman, G. C., Penders, J. E. & Burne, R. A. (1997). Transcriptional analysis of the Streptococcus mutans hrcA, grpE and dnaK genes and regulation of expression in response to heat shock and environmental acidification. Mol Microbiol 25, 329-341. Jenkinson, H. F. & Demuth, D. R. (1997). Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol 23, 183-190. Jensen, M. E. & Wefel, J. S. (1989). Human plaque pH responses to meals and the effects of chewing gum. Br Dent J 167, 204-208. Johnson, C. P., Gross, S. M. & Hillman, J. D. (1980). Cariogenic potential in vitro in man and in vivo in the rat of lactate dehydrogenase mutants of Streptococcus mutans. Arch Oral Biol 25, 707-713. Kakinuma, Y. (1998). Inorganic cation transport and energy transduction in Enterococcus hirae and other streptococci. Microbiol Mol Biol Rev 62, 1021-1045. Kaku, M., Yagawa, K., Nagao, S. & Tanaka, A. (1983). Enhanced superoxide anion release from phagocytes by muramyl dipeptide or lipopolysaccharide. Infect Immun 39, 559-564. Kierek, K. & Watnick, P. I. (2003). The Vibrio cholerae O139 O-antigen polysaccharide is essential for Ca2+-dependent biofilm development in sea water. Proc Natl Acad Sci U S A 100, 14357-14362. Kihlberg, B. M., Cooney, J., Caparon, M. G., Olsen, A. & Bjorck, L. (1995). Biological properties of a Streptococcus pyogenes mutant generated by Tn916 insertion in mga. Microb Pathog 19, 299-315. Kitten, T., Munro, C. L., Michalek, S. M. & Macrina, F. L. (2000). Genetic characterization of a Streptococcus mutans LraI family operon and role in virulence. Infect Immun 68, 4441-4451. Kleinberg, I. (2002). A mixed-bacteria ecological approach to understanding the role of the oral bacteria in dental caries causation: an alternative to Streptococcus mutans and the specific-plaque hypothesis. Crit Rev Oral Biol Med 13, 108-125. Kloosterman, T. G., Hendriksen, W. T., Bijlsma, J. J., Bootsma, H. J., van Hijum, S. A., Kok, J., Hermans, P. W. & Kuipers, O. P. (2006). Regulation of glutamine and glutamate metabolism by GlnR and GlnA in Streptococcus pneumoniae. J Biol Chem 281, 25097-25109. Klose, K. E. & Mekalanos, J. J. (1997). Simultaneous prevention of glutamine synthesis and high-affinity transport attenuates Salmonella typhimurium virulence. Infect Immun 65, 587-596. Kobayashi, H., Suzuki, T. & Unemoto, T. (1986). Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-translocating ATPase. J Biol Chem 261, 627-630. Koebmann, B. J., Nilsson, D., Kuipers, O. P. & Jensen, P. R. (2000). The membrane-bound H(+)-ATPase complex is essential for growth of Lactococcus lactis. J Bacteriol 182, 4738-4743. Kohler, B., Birkhed, D. & Olsson, S. (1995). Acid production by human strains of Streptococcus mutans and Streptococcus sobrinus. Caries Res 29, 402-406. Kolenbrander, P. E. & London, J. (1993). Adhere today, here tomorrow: oral bacterial adherence. J Bacteriol 175, 3247-3252. Kolenbrander, P. E., Palmer, R. J., Jr., Rickard, A. H., Jakubovics, N. S., Chalmers, N. I. & Diaz, P. I. (2006). Bacterial interactions and successions during plaque development. Periodontol 2000 42, 47-79. Korithoski, B., Krastel, K. & Cvitkovitch, D. G. (2005). Transport and metabolism of citrate by Streptococcus mutans. J Bacteriol 187, 4451-4456. Kreikemeyer, B., McIver, K. S. & Podbielski, A. (2003). Virulence factor regulation and regulatory networks in Streptococcus pyogenes and their impact on pathogen-host interactions. Trends Microbiol 11, 224-232. Kremer, B. H., van der Kraan, M., Crowley, P. J., Hamilton, I. R., Brady, L. J. & Bleiweis, A. S. (2001). Characterization of the sat operon in Streptococcus mutans: evidence for a role of Ffh in acid tolerance. J Bacteriol 183, 2543-2552. Krom, B. P., Warner, J. B., Konings, W. N. & Lolkema, J. S. (2000). Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis. J Bacteriol 182, 6374-6381. Krom, B. P., Huttinga, H., Warner, J. B. & Lolkema, J. S. (2002). Impact of the Mg2+-citrate transporter CitM on heavy metal toxicity in Bacillus subtilis. Arch Microbiol 178, 370-375. Kuhnert, W. L., Zheng, G., Faustoferri, R. C. & Quivey, R. G., Jr. (2004). The F-ATPase operon promoter of Streptococcus mutans is transcriptionally regulated in response to external pH. J Bacteriol 186, 8524-8528. Kullen, M. J. & Klaenhammer, T. R. (1999). Identification of the pH-inducible, proton-translocating F1F0-ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: gene structure, cloning and characterization. Mol Microbiol 33, 1152-1161. Kuramitsu, H. K. (1993). Virulence factors of mutans streptococci: role of molecular genetics. Crit Rev Oral Biol Med 4, 159-176. Larsen, R., Kloosterman, T. G., Kok, J. & Kuipers, O. P. (2006). GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis. J Bacteriol 188, 4978-4982. Lau, G. W., Haataja, S., Lonetto, M., Kensit, S. E., Marra, A., Bryant, A. P., McDevitt, D., Morrison, D. A. & Holden, D. W. (2001). A functional genomic analysis of type 3 Streptococcus pneumoniae virulence. Mol Microbiol 40, 555-571. Lau, P. C., Sung, C. K., Lee, J. H., Morrison, D. A. & Cvitkovitch, D. G. (2002). PCR ligation mutagenesis in transformable streptococci: application and efficiency. J Microbiol Methods 49, 193-205. Lee, S. F. & Boran, T. L. (2003). Roles of sortase in surface expression of the major protein adhesin P1, saliva-induced aggregation and adherence, and cariogenicity of Streptococcus mutans. Infect Immun 71, 676-681. Lee, S. F., Delaney, G. D. & Elkhateeb, M. (2004). A two-component covRS regulatory system regulates expression of fructosyltransferase and a novel extracellular carbohydrate in Streptococcus mutans. Infect Immun 72, 3968-3973. Lee, Y. L., Thrupp, L., Owens, J., Cesario, T. & Shanbrom, E. (2001). Bactericidal activity of citrate against Gram-positive cocci. Lett Appl Microbiol 33, 349-351. Lemos, J. A., Chen, Y. Y. & Burne, R. A. (2001). Genetic and physiologic analysis of the groE operon and role of the HrcA repressor in stress gene regulation and acid tolerance in Streptococcus mutans. J Bacteriol 183, 6074-6084. Lemos, J. A. & Burne, R. A. (2002). Regulation and physiological significance of ClpC and ClpP in Streptococcus mutans. J Bacteriol 184, 6357-6366. Lemos, J. A., Brown, T. A., Jr. & Burne, R. A. (2004). Effects of RelA on key virulence properties of planktonic and biofilm populations of Streptococcus mutans. Infect Immun 72, 1431-1440. Len, A. C., Harty, D. W. & Jacques, N. A. (2004a). Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance. Microbiology 150, 1353-1366. Len, A. C., Harty, D. W. & Jacques, N. A. (2004b). Stress-responsive proteins are upregulated in Streptococcus mutans during acid tolerance. Microbiology 150, 1339-1351. Levesque, C. M., Mair, R. W., Perry, J. A., Lau, P. C., Li, Y. H. & Cvitkovitch, D. G. (2007). Systemic inactivation and phenotypic characterization of two-component systems in expression of Streptococcus mutans virulence properties. Lett Appl Microbiol 45, 398-404. Li, H. & Pajor, A. M. (2002). Functional characterization of CitM, the Mg2+-citrate transporter. J Membr Biol 185, 9-16. Li, Y. & Burne, R. A. (2001). Regulation of the gtfBC and ftf genes of Streptococcus mutans in biofilms in response to pH and carbohydrate. Microbiology 147, 2841-2848. Li, Y. H., Hanna, M. N., Svensater, G., Ellen, R. P. & Cvitkovitch, D. G. (2001a). Cell density modulates acid adaptation in Streptococcus mutans: implications for survival in biofilms. J Bacteriol 183, 6875-6884. Li, Y. H., Lau, P. C., Lee, J. H., Ellen, R. P. & Cvitkovitch, D. G. (2001b). Natural genetic transformation of Streptococcus mutans growing in biofilms. J Bacteriol 183, 897-908. Li, Y. H., Lau, P. C., Tang, N., Svensater, G., Ellen, R. P. & Cvitkovitch, D. G. (2002a). Novel two-component regulatory system involved in biofilm formation and acid resistance in Streptococcus mutans. J Bacteriol 184, 6333-6342. Li, Y. H., Tang, N., Aspiras, M. B., Lau, P. C., Lee, J. H., Ellen, R. P. & Cvitkovitch, D. G. (2002b). A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation. J Bacteriol 184, 2699-2708. Lie, T. (1977). Early dental plaque morphogenesis. A scanning electron microscope study using the hydroxyapatite splint model and a low-sucrose diet. J Periodontal Res 12, 73-89. Liew, F. Y., Millott, S., Parkinson, C., Palmer, R. M. & Moncada, S. (1990). Macrophage killing of Leishmania parasite in vivo is mediated by nitric oxide from L-arginine. J Immunol 144, 4794-4797. Lim, E. M., Ehrlich, S. D. & Maguin, E. ( | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37239 | - |
dc.description.abstract | 轉糖鏈球菌是一株經常在人類口腔中被發現的革蘭氏陽性兼性厭氧菌,其主要會造成牙齒的蛀蝕。自最初的菌落開始形成,轉糖鏈球菌必須對其所經歷連續的動態挑戰產生反應與適應,轉糖鏈球菌必須應付包括酸、營養物和必需元素的需求、巨噬細胞殺害等壓力。本篇研究重點在於轉糖鏈球菌面對金屬離子、弱酸與過氧化氫所產生的反應與基因調控。本研究的第一部分是關於口腔中的混合填充物會因腐蝕而釋出二價銅離子,發現二價銅離子會專一性的針對葡萄糖傳遞酶中的gtfD基因增加轉錄表現量,此調控與運送二價銅離子的copYAZ操縱子無關,顯示二價銅離子是調控gtfD基因的重要因子。再者發現酸對於葡萄糖傳遞酶的基因轉錄表現調控會受營養物的不同而改變,但卻不影響二價銅離子的調控。除了調控特定基因的表現之外,細菌還發展全面性的適應調控系統去面對一直變動的環境挑戰與助其生存於壓力環境中。轉糖鏈球菌面對弱酸的反應是本研究的第二部分。總體的調控系統可控制大量基因產生同步面臨環境壓力的表現,本部分之研究主要利用電腦和微陣列分析,發現受酸降低表現的操縱子中,GlnR box (ATGTNAN7TNACAT)位於轉糖鏈球菌參與胺基酸生合成與運送基因的控制子中,進一步發現參與胺基酸代謝的基因群會受到GlnR的負調控,而剔除此序列則使citBZC操縱子受酸減少轉錄表現的現象消失,因此結果顯示轉糖鏈球菌面對酸時會減少胺基酸前驅物的產生。已知三羧酸循環的中間代謝產物可經由專一的運送者輸入細菌體內,研究發現檸檬酸鹽的最高運送效率是經由CitM以質子電化學梯度驅動,利用一個質子與一個鈣離子-檸檬酸鹽複合物所形成電中性運送,結果顯示轉糖鏈球菌受外界檸檬酸鹽刺激可增加運送能力並增加在弱酸環境的生存能力,因此結果顯示檸檬酸鹽可調控轉糖鏈球菌的耐酸能力。第三部分的研究是關於轉糖鏈球菌對於過氧化氫的反應和基因調控。主要研究為轉糖鏈球菌如何保護自身對抗宿主之防禦系統並維持其於口腔中的生態。研究顯示,一對ScnR/ScnK雙組成系統可能參與調控細菌與細胞間作用機制。結果顯示無論野生株和突變株皆會被老鼠的巨噬細胞RAW 264.7吞噬,且巨噬細胞對於scnRK-null突變株的胞內敏感性增加。當活化的巨噬細胞吞噬野生株後,其活性氧的濃度明顯降低,但這樣的結果卻無法於突變株上觀察到,顯示由於中和活性氧的能力降低使得巨噬細胞的毒殺效果增加。此外,scnR-和scnRK-null對於過氧化氫的敏感性皆比野生株增加。有趣的是,scnRK基因的表現並不受過氧化氫所影響。轉糖鏈球菌的ScnRK基因在中和氧化壓力方面扮演一重要角色,並參與一部份抑制胞內活性氧生成而可減低被吞噬細胞毒殺之敏感性。 | zh_TW |
dc.description.abstract | Streptococcus mutans is a Gram-positive, facultatively anaerobic bacteria commonly found in the human oral cavity and is a significant contributor to the caries on tooth. From initial colonization stages onward, S. mutans undergoes continuous dynamic challenges to which it must respond and adapt. S. mutans have to deal with stresses such as acidity, nutrients, essential elements, and the macrophage killing. The stress responses and gene regulation of S. mutans against metal ions, low pH and hydrogen peroxide (H2O2) were examined. In part I, copper ion (Cu2+) was released through corrosion of amalgam fillings in the oral cavity. The transcriptional expression of gtfD but not gtfB and gtfC was specifically induced by Cu2+ and independent of the Cu2+-transport operon copYAZ. Nutrient change influences the effect of pH not Cu2+. In addition to the expression regulation of specific genes, bacteria have evolved adaptive networks to face the challenges of a changing environment and to survive under conditions of stress. The response of S. mutans to low pH was investigated in the second part. The global regulatory systems control the simultaneous expression of a large number of genes in response to a variety of environmental stress factors. The GlnR box (ATGTNAN7TNACAT) was found in the promoter regions of acid-repressed operons involving in amino acid biosynthesis and transport in S. mutans based on microarray and in silico analyses. The expression of the amino acid metabolism clusters was negatively regulated by GlnR and deletion of this motif abolished the acidic repression of the citBZC operon. S. mutans reduced the production of amino acid precursors in response to acidity. The intermediate metabolites of TCA cycle could be specifically imported by transporters. The highest citrate transport rate of CitM was by the proton electrochemical gradient and supports an electroneutral transport mechanism with a coupling stoichiometry of one proton and one (Ca2+-citrate)1- complex. The citrate transport activity and survival of S. mutans at low pH are induced by the presence of citrate in the medium. The citrate modulates S. mutans aciduricity. Thirdly, the response and gene regulation of S. mutans to H2O2 was investigated. S. mutans avoids possible host defenses and maintain its ecological niche in the oral cavity. A putative two-component system (TCS), ScnR/ScnK, was invovled in the mechanisms underlying bacteria-cellular interaction. Both the wild-type and mutant strains were phagocytosed by murine macrophage RAW 264.7 cells at a comparable rate and an increased intracellular susceptibility was observed with the scnRK-null mutants. The amount of reactive oxygen species (ROS) in activated macrophages was reduced significantly after ingesting wild-type, but not scnRK-null mutant strains. This suggests that increased macrophage killing of these mutants is due to the impaired ability to counteract ROS. Additionally, both scnR- and scnRK-null mutants were more susceptible to H2O2. It is of interest to find that scnRK expression was unaffected by H2O2. ScnRK is important in counteracting oxidative stress in S. mutans. The decreased susceptibility to phagocytic killing is at least partly attributable to inhibition of intracellular ROS formation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:22:13Z (GMT). No. of bitstreams: 1 ntu-97-D90445004-1.pdf: 2158877 bytes, checksum: 03fde00a6427b804688e7101fbeb40ca (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | TABLE OF CONTENTS……………………………………………….…i
LIST OF FIGURES………………………………………………………iv LIST OF TABLES………………………………………………………...v ABSTRACT (in Chinese).………………………………………………..vi ABSTRACT (in English).………………………………………………viii LIST OF PUBLICATIONS………………………………………………x LIST OF ABBREVIATIONS……………………………………………xi Chapter 1. INTRODUCTION……………………………………………1 1. Dental plaque……………………………………………………….……2 1.1. Streptococcus mutans……………………………………………….…2 1.2. Dental caries…………………………………………………………...3 2. The adhesion of S. mutans……………………………………………….4 2.1. The sucrose independent attachment…………………………………..5 2.2. The sucrose dependent attachment…………………………………….6 2.2.1. The GTFs…………………………………………………………….6 2.3. The other adhesins……………………………………………………..8 3. Biofilm formation……………………………………………………….9 3.1. The uptake of metals…………………………………………………10 4. Carbohydrate metabolism………………………………………………11 4.1. Acidogenicity………………………………………………………...11 5. Acid-tolerance………………………………………………………….12 5.1. Acid stress response………………………………………………….13 5.2. pH adaptation of S. mutans…………………………………………..13 5.2.1. Maintaining intracellular pH……………………………………….14 5.2.2. Repair mechanisms…………………………………………………16 5.2.2.1. DNA repair system………………………………………………16 5.2.2.2. Chaperones……………………………………………………….17 5.2.3. Changes in cell membrane composition……………………………17 5.2.4. Regulators………………………………………………………….18 5.2.5. Bioflim……………………………………………………………...19 5.2.6. Other facets of acid adaptation……………………………………..20 6. Nitrogen source………………………………………………………...21 6.1. The citrate pathway…………………………………………………..22 6.2. The glutamate biosynthesis…………………………………………..22 6.3. The link between carbon and nitrogen metabolism…………………..23 6.4. The citrate metabolism and transport………………………………...23 6.4.1. The citrate metabolism……………………………………………..23 6.4.2. The genetic organization of the citrate fermentation clusters……...24 6.4.3. The citrate transport………………………………………………...24 7. Defense system of the oral cavity………………………………………27 7.1. The natural antimicrobial function of saliva…………………………28 7.2. The oral neutrophils…………………………………………………..30 7.3. The oxidative stress…………………………………………………..31 7.4. Bacterial reactive oxygen species (ROS) production and their defense mechanism………………………………………………………………...31 8. Endocarditis…………………………………………………………….32 8.1. TCSs and bacterial virulence…………………………………………33 Chapter 2. AIMS OF STUDY…………………………………………...35 Chapter 3. MATERIALS AND METHODS…………………………...38 1. Bacterial strains and growth conditions………………………………..39 1.1. S. mutans strains……………………………………………………...39 1.2. E. coli strains…………………………………………………………39 2. Construction of S. mutans mutants……………………………………..39 3. General genetic manipulations…………………………………………40 3.1. DNA manipulation and Southern blot analysis………………………41 3.2. RNA manipulation……………………………………………………41 3.2.1. Northern blot analysis……………………………………………...41 3.2.2. Reverse transcription PCR (RT-PCR) ……………………………..42 4. Western blot analysis…………………………………………………...42 5. In vitro sucrose-dependent adherence assay……………………………43 6. Microarray hybridization and analysis…………………………………43 7. The citrate transport assay……………………………………………...44 8. Assay for acid adaptation in planktonic batch cultures………………...45 9. Quantitative assessment of bacterial phagocytosis and killing in macrophages………………………………………………………………46 10. ROS detection…………………………………………………………47 11. Assay for sensitivity of S. mutans to H2O2.……………….…………..47 Chapter 4. RESULTS……………………………………………………48 PART. I……………………………………………………………………49 1. The expression of gtf genes…………………………………………….49 1.1. Maps and specificity of anti-sense gtf RNA probes………………….49 1.2. Effect of medium on the expression of gtf genes in response to low pH…………………………………………………………………………50 1.3. Effect of ions on the expression of gtf genes…………………………51 1.4. Effect of Cu2+ in the defined medium on the expression of gtf………51 2. Effect of the cop operon on the expression of gtf genes in response to Cu2+………………………………………………………………………51 3. Expression of GTFD is increased in response to Cu2+…………………52 4. Effects of Cu2+ on the adherence of S. mutans GS-5, GS-5DD and NHS1……………………………………………………………………...52 PART. II…………………………………………………………………...53 1. Transcriptome analysis of pH 5.5-grown S. mutans……………………53 2. Identification of a regulatory motif among acid-repressed genes……...55 3. The palindromic sequence is involved in regulation of citBZC at acidic pH…………………………………………………………………………56 4. Functional characterization of CitM……………………………………57 4.1. Divalent metal ions effects on citrate uptake by CitM……………….57 4.2. Substrate specificity of CitM…………………………………………57 4.3. Effect of pH on citrate uptake by CitM………………………………58 4.4. Effect of ionophores on citrate uptake in CitM………………………58 5. The uptake of citrate is increased by citrate……………………………59 5.1. Adaptation to citrate enhances survival at low pH…………………...59 5.2. Transcriptome analysis of S. mutans grown in TVG±citrate………...59 PART. III…………………………………………………………………..60 1. Identification of the S. mutans scnRK locus……………………………60 2. Phenotypical characterization of scnRK mutants………………………61 3. Role of scnRK in resistance to phagocytic killing……………………...62 4. Role of scnRK in counteracting ROS in vivo…………………………..62 5. Requirement of ScnRK for resistance to H2O2…………………………63 6. Transcriptional regulation of the redox related genes in response to H2O2………………………………………………………..……………...64 Chapter 5. DISCUSSION……………………………………………….65 PART. I……………………………………………………………………66 PART. II…………………………………………………………………...71 PART. III…………………………………………………………………..73 Chapter 6. CONCLUSION……………………………………………...80 REFERENCES…………………………………………………………..82 FIGURES……………………………………………………………….127 FIG. 1. Maps and specificity of anti-sense RNA probes………………..128 FIG. 2. Effect of medium on the expression of gtf genes in response to low pH………………………………………………………………………..129 FIG. 3. Effect of ions on the expression of gtf genes…………………...130 FIG. 4. Effect of Cu2+ in the defined medium on the expression of gtf genes……………………………………………………………………..131 FIG. 5. Effect of the cop operon on the expression of gtf genes in response to Cu2+…………………………………………………………………...132 FIG. 6. The induction of GTFD in culture supernatants by Cu2+……….133 FIG. 7. Comparison of microarray and RT-PCR results for twenty selected genes……………………………………………………………………..134 FIG. 8. GlnR was involved in the acid-repression of amino acid metabolism clusters in S. mutans………………………………………..135 FIG. 9. The palindromic sequence was involved in the regulation of citBZC at acidic pH……………………………………………………...136 FIG. 10. The citrate uptake by CitM expressed in E. coli……………….137 FIG. 11. The effect of citrate in S. mutans………………………………138 FIG. 12. Genomic analysis of GS-RK and Xc-RK……………………...139 FIG. 13. Phagocytosis and killing assays……………………………….140 FIG. 14. Detection and inhibition of intracellular ROS…………………141 FIG. 15. Effect of H2O2 on scnRK and bacterial survival.........................142 FIG. 16. H2O2 induced expression of anti-oxidative genes……………..143 TABLES………………………………………………………………...144 TABLE 1. Bacterial strains and plasmids used in this study……………145 TABLE 2. Primers used in this study…………………………………...147 TABLE 3. Up-regulated genes during acidic pH………………………..151 TABLE 4. Down regulated genes during acidic pH…………………….155 TABLE 5. Genes differentially expressed grown in TVG±citrate……...161 APPENDIX……………………………………………………………..163 FIG. A. Streptococcal carbohydrate metabolism and the associated dvicious cycleT of acidification…………………………………………………...164 FIG. B. Amino acid biosynthesis and transport…………………………165 FIG. C. Citrate-metabolic pathways…………………………………….166 | |
dc.language.iso | en | |
dc.title | 轉糖鏈球菌對酸,氧化及金屬離子壓力的基因調控 | zh_TW |
dc.title | Transcriptional regulation of acid, oxidative and metal ion
stress responses in Streptococcus mutans | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 賴辰雄,錢佑,陳振陽,鄧述諄,陳怡原 | |
dc.subject.keyword | 轉糖鏈球菌,二價銅離子,弱酸,過氧化氫, | zh_TW |
dc.subject.keyword | Streptococcus mutans,copper ion,low pH,hydrogen peroxide, | en |
dc.relation.page | 166 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-23 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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