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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 賈景山 | |
dc.contributor.author | Wei-Chung Lin | en |
dc.contributor.author | 林韋君 | zh_TW |
dc.date.accessioned | 2021-06-13T15:20:15Z | - |
dc.date.available | 2010-08-14 | |
dc.date.copyright | 2008-08-14 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-23 | |
dc.identifier.citation | Arunachalam, K., Gill, H.S., and Chandra, R.K. (2000). Enhancement of natural immune function by dietary consumption of Bifidobacterium lactis (HN019). European journal of clinical nutrition 54, 263-267.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37156 | - |
dc.description.abstract | 吞噬作用是當感染原進入宿主體內時,最先引起的先天性免疫反應,而嗜中性球及巨噬細胞則扮演了主要吞噬感染原的角色。之前許多研究指出,細菌發酵產物具有免疫調節功能,且在服用乳酸菌的發酵產物後,受測者周邊血液中單核球的吞噬能力,有明顯上升的現象。在本研究中,我們建立體外的免疫細胞吞噬作用模式,測試豆類細菌發酵產物是否具有直接增強細胞吞噬能力。先以4%無菌硫乙醇酸鹽由腹腔注射老鼠,隔天分離出腹水細胞,包括嗜中性球及巨噬細胞,以細菌發酵產物刺激不同時間點後,加入帶有綠色螢光蛋白的大腸桿菌,利用流式細胞儀分析腹水細胞的吞噬能力,並利用H DCFA檢測細胞內活性氧的產生量。結果顯示細菌發酵產物分子量大於50 kDa的部分,具有促進腹水吞噬細胞及人類單核球細胞株THP-1吞噬作用的效果,此外,分子量小於50 kDa的細菌發酵產物抑制脂多醣體或大腸桿菌所引起小鼠腹水吞噬細胞及人類單核球細胞株的活性氧化物產生。本實驗結果顯示,細菌發酵產物具多重活性,除了可促進吞噬細胞的功能外,也可調節氧化壓力的過度產生。 | zh_TW |
dc.description.abstract | Phagocytosis is the first line of host defense against pathogens and is mediated by phagocytes including macrophages and neutrophils. The foods fermented products of lactic acid bacteria have the capacity to modulate immune function. In animals feeding with lactic acid bacteria fermented products, the phagocytic activity of peripheral blood mononuclear cells could be enhanced. In this study, we established an in vitro phagocytosis assay to investigate whether a soybean fermented has a direct phagocytosis-stimulating effects. After intra-peritoneal injected with 4% thioglycollate overnight, Balb/c mice were sacrificed and the peritoneal cells, neutrophils and macrophages were collected. Following stimulation with soybean fermented product, these phagocytic cells were challenged with GFP-expressed E. coli. Phagocytosis was semi-quantitated by flow cytometry at different time intervals. In parallel experiments, ROS production by primary phagocytes or cell lines was measured by H DCFA. The fermented products or its fractions harboring componnts greater than 50 kDa could enhance the phagocytic ability of mouse phagocytes or human macrophage cell line, THP-1. On the other hands, the fractions less than 50 kDa could inhibit LPS- or bacteria-induced ROS production in mouse phagocytes or THP-1. The results indicated that the soybean fermented product exhibit complex activities from enhancement of the phagocytosis to the regulation of oxidative stress in phagocytic cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:20:15Z (GMT). No. of bitstreams: 1 ntu-97-R95445125-1.pdf: 1739524 bytes, checksum: 49ca2a146b34361b4ba07d8039089da5 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 中文摘要---------------------------------------------------1
英文摘要---------------------------------------------------2 第一章、緒論-----------------------------------------------6 一、宿主先天性免疫反應(Innate immune system)---------------6 二、吞噬細胞(phagocytic cells):巨噬細胞(macrophages)與嗜中性球(neutrophils)------------------------------------------7 三、吞噬作用(phagocytosis)---------------------------------9 四、吞噬細胞活性氧化物(Reactive oxygen species)之產生-----10 五、活性氧化物的危害--------------------------------------10 六、乳酸菌發酵產物(Lactic acid bacteria fermented product)---11 1.豆類及其發酵產物(soybean fermented product)---------------------------11 2.乳酸菌及乳酸發酵產物之非專一性免疫調節----------------------------13 2.1吞噬作用(phagocytosis)------------------------------------------------------13 2.2自然殺手細胞(Natural killer cell)------------------------------------------13 2.3抗過敏---------------------------------------------------------------------------14 3.乳酸菌及乳酸菌發酵產物之專一性免疫調節----------------------------15 3.1刺激免疫球蛋白A (Immunoglobulin A)的產生-------------------------15 3.2樹突狀細胞(Dendritic cells)及調節性T細胞(Regulatory T cells)----16 第二章、 實驗方法與材料-------------------------------------------------------18 一、細菌發酵產物來源-----------------------------------------------------------18 二、人類單核球細胞株THP-1之培養----------------------------------------18 三、分離小鼠腹腔中的腹水細胞(peritoneal cell)---------------------------18 四、分離人類多形核白血球(polymorphonuclear cell)----------------------19 五、小鼠腹水細胞表面標記(surface marker)的螢光染色-----------------19 六、吞噬作用檢驗系統(phagocytosis assay system)------------------------20 七、活性氧化物(Reactive Oxygen Species, ROS)測定----------------------20 八、大腸桿菌生長曲線測定-----------------------------------------------------21 九、人類單核球細胞株THP-1之殺菌作用----------------------------------21 十、細菌發酵產物的初步純化--------------------------------------------------22 第三章、 結果------------------------------------------------------------------23 一、確認小鼠腹水細胞種類-----------------------------------------------------23 二、細菌發酵產物對於小鼠腹水細胞吞噬作用之測試--------------------23 三、流式細胞儀之數據分析-----------------------------------------------------24 四、細菌發酵產物之初步純化-------------------------------------------------24 五、細菌發酵產物對人類單核球細胞株THP-1吞噬作用之影響-------25 六、細菌發酵產物對於人類多形核白血球吞噬作用之測試-------------25 七、細菌發酵產物對小鼠腹水細胞及人類單核球細胞株THP-1活性氧化物產生之影響-------------------------------------------------------------26 八、異黃酮物質對於小鼠腹水細胞吞噬作用之影響----------------------27 九、細菌發酵產物對於大腸桿菌生長之影響--------------------------------27 十、細菌發酵產物對人類單核球細胞株THP-1殺菌作用之影響-------28 十一、利用陰離子交換樹脂(Anion Exchange)純化細菌發酵產物-------28 第四章、討論-----------------------------------------------------------------------29 第五章、參考文獻----------------------------------------------------------------32 第六章、圖表-----------------------------------------------------------------------41 圖一、小鼠腹水細胞之表面標記-----------------------------------------------41 圖二、流式細胞儀之數據分析--------------------------------------------------42 圖三、細菌發酵產物刺激小鼠腹水細胞的不同時間吞噬效果-----------43 圖四、細菌發酵產物分子量大於50 kDa及小於50 kDa的吞噬作用檢驗------------------------------------------------------------------------------44 圖五、細菌發酵產物對人類多形核白血球吞噬作用之影響-------------45 圖六、細菌發酵產物及異黃酮對小鼠腹水細胞活性氧化物產生之影響------------------------------------------------------------------------------46 圖七、細菌發酵產物及異黃酮對於人類單核球細胞株產生活性氧化物之影響-----------------------------------------------------------------------47 圖八、異黃酮物質對於小鼠腹水細胞吞噬作用的影響------------------48 圖九、細菌發酵產物對於大腸桿菌生長之影響---------------------------49 圖十、細菌發酵產物對於人類單核球細胞株殺菌之影響---------------50 圖十一、Q-管柱分出之曲線圖------------------------------------------------51 表一、Q-管柱純化片段之吞噬作用能力測試------------------------------51 第七章、附圖---------------------------------------------------------------------52圖一、活性氧化物之產生及其參與之細胞功能-----------------------------52 | |
dc.language.iso | zh-TW | |
dc.title | 細菌發酵產物調節吞噬細胞之吞噬及活性氧化物產生之能力 | zh_TW |
dc.title | Modulation of Phagocytic Cell Phaocytosis and Reactive Oxygen Species Production by Bacteria Fermentation Product | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王萬波,鄧述諄 | |
dc.subject.keyword | 大豆發酵產物,吞噬細胞,吞噬作用,活性氧分子,抗氧化物, | zh_TW |
dc.subject.keyword | Soybean fermentation product,phagocytic cells,phagocytosis,ROS,antioxidant, | en |
dc.relation.page | 52 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-24 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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