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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 宋孔彬(Kung-Bin Sung) | |
dc.contributor.author | Po-Jen Lai | en |
dc.contributor.author | 賴柏禎 | zh_TW |
dc.date.accessioned | 2021-06-13T15:19:18Z | - |
dc.date.available | 2013-07-26 | |
dc.date.copyright | 2008-07-26 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-24 | |
dc.identifier.citation | 1. 陳健尉, '二十一世紀基因分析的利器:基因微陣列簡介及其應用', 生物醫學報導, (2000).
2. 陳怡君, '利用光學消逝波偵測的新型生物晶片掃瞄系統', 國立台灣大學電機工程研究所碩士論文, (2007). 3. Christian D., Gero V.P, “Optical Effects of Metallic Nanoparticles”, Australian journal of chemistry, (2007). 4. Haaland, D. M., J. A. Timlin, et al. “Multivariate curve resolution for hyperspectral image analysis: Applications to microarray technology”, San Jose, CA, United States, The International Society for Optical Engineering, (2003). 5. Reichert J., Csaki A., Kohler JM., Fritzsche W., “Chip-Based Optical Detection of DNA Hybridization by Means of Nanobead Labeling”, ANALYTICAL CHEMISTRY, (2000). 6. Bao P., Frutos A.G., Greef C., et al. “High-Sensitivity Detection of DNA Hybridization on Microarrays Using Resonance Light Scattering”, ANALYTICAL CHEMISTRY, (2002). 7. Yguerabide, J. and E. E. Yguerabide. 'Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications - I. Theory.' Analytical Biochemistry 262(2): 137-156(1998). 8. 蘇正翔, ”近紅外影像應用於白米內部品質檢測”, 國立台灣大學生物產業機電工程學研究所碩士論文, (2003). 9. Eugene Hecht, “OPTICS”, 4th ed., Addsion Wesley, Boston., 2002. 10. Umran S. Inan, Aziz S. Inan “Engineering Electromagnetics“, 11. 鍾錫華, ”光學 “, 儒林圖書公司, (1997). 12. Kauppinen J , “Fourier Transforms in Spectroscopy”, WILEY-VCH, (2001) 13. Oldenburg, Amy L., Xu, Chenyang , Boppart, Stephen A., ” Spectroscopic optical coherence tomography and microscopy”, IEEE, (2007). 14. CURRY A , ” Substrate effect on refractive index dependence of plasmon resonance for individual silver nanoparticles observed using darkfield micro-spectroscopy ”, OPTICS EXPRESS, (2005). 15. LIU GL, ” High-speed multispectral imaging of nanoplasmonic array ”, OPTICS EXPRESS, (2005). 16. James H.M., Ronald W.S, Mark A.Y, “Signal Processing First”, Prentice Hall, (2003). 17. Steven W.S., “The Scientist and Engineer's Guide to Digital Signal Processing”, p.423-p.429 (1998). 18. R D Alcock and J M Coupland , “A compact, high numerical aperture imaging Fourier transform spectrometer and its application”, INSTITUTE OF PHYSICS PUBLISHING, (2006) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37106 | - |
dc.description.abstract | 本研究為架設ㄧ新的光學掃描系統,可大面積且快速的獲得玻片表面上金屬奈米粒子之光譜。
以傅立葉轉換光譜儀為基礎架設ㄧ多光譜影像系統。藉由改變Michelson干涉儀中兩道光束之光路程差產生光譜的資訊,利用電荷耦合器(CCD)擷取干涉強度,並表示成隨光路程差產生強度變化之干涉圖形(interferogram),最後再對干涉圖形做傅立葉轉換獲得光譜。目前本光學系統已可獲得白光與不同filter產生之光譜以及兩種金屬奈米粒子之散射光譜。最終目標是希望能應用在以金屬奈米粒子所標記之基因晶片上,達到大面積、高解析度與快速檢測的目的。 最後與Berkeley大學Dr. Lee團隊所架設之多光譜影像系統做比較,其系統獲得300 µm 300 µm視野內所有金屬奈米粒子之光譜所需時間約為5分鐘;而本系統掃瞄視野大小465 µm 617 µm約為80秒。相較於Dr. Lee團隊,本系統在光譜解析度與掃描時間上都有改善的空間,需更努力以達到比他們更好的效果。 | zh_TW |
dc.description.abstract | In this thesis, we present a novel optical scanning system for rapidly detecting scattering spectra of metal nanoparticles within large field of view on a slide.
We constructed a hyperspectral imaging system based on Fourier transform spectrometry. Spectral information was generated by changing optical path difference between two beams in a Michelson interferometer. We utilized charge couple detector to record interference intensity as a function of the optical path difference (interferogram), and calculated the spectra with Fourier transformed the interferogram. By our optical system, we successfully obtained spectral information from white light source with different filters, and scattering spectra from two types of metal nanoparticles on glass slides. The ultimate goal is to apply our optical system to fast scan the scattering spectra of the metal nanoparticles used as optical tags in the microarray. For benchmarking, we compared our results with the multispectral microscopic imaging system built by Dr. Lee’s group at the university of California-Berkeley. Their measuring time of the scattering spectra of metal nanoparticles within the 300 µm x 300 µm field of view is 5 min and ours within the 465 µm x 617 µm field of view is approximately 80 seconds. Compared with Dr. Lee’s group, we have improvable performance in spectral resolution as well as scanning time, and need further effort to get beyond them. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:19:18Z (GMT). No. of bitstreams: 1 ntu-97-R95921102-1.pdf: 2097579 bytes, checksum: e9b6811a77172717a3308f1c5319094e (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 致謝...........................I
中文摘要......................II 英文摘要.....................III 目錄..........................IV 表目錄........................VI 第一章 緒論...................1 1-1前言........................1 1-2研究動機....................2 1-3論文架構....................3 第二章 理論基礎...............5 2-1光波........................5 2-2光的的疊加與干涉原理........7 2-3同調程度...................13 2-4傅立業轉換光譜儀...........19 2-5傅立業轉換.................21 2-6文獻回顧...................22 第三章 研究方法..............26 3-1實驗架構...................26 3-2系統架設與校正.............29 第四章 實驗結果..............33 4-1雷射光干涉實驗結果.........33 4-2寬頻光干涉實驗結果.........35 4-3金屬奈米粒子實驗結果.......39 第五章 討論與結論............49 參考文獻......................51 | |
dc.language.iso | zh-TW | |
dc.title | 利用傅立葉轉換光譜影像技術快速測量金屬奈米粒子散射光譜 | zh_TW |
dc.title | Fast measurement of light scattering spectra from metal nanoparticles based on Fourier transform imaging spectrometry | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 莊曜宇,黃志清 | |
dc.subject.keyword | 傅立葉轉換光譜儀,金屬奈米粒子,暗場聚光鏡,散射光譜, | zh_TW |
dc.subject.keyword | Fourier transform spectrometer,Metal nanoparticles,darkfield condenser,scattering spectra, | en |
dc.relation.page | 52 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-07-24 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 電機工程學研究所 | zh_TW |
顯示於系所單位: | 電機工程學系 |
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