請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37096
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林俊彬(Chun-Pin Lin) | |
dc.contributor.author | Ying-Chieh Huang | en |
dc.contributor.author | 黃瑩潔 | zh_TW |
dc.date.accessioned | 2021-06-13T15:19:08Z | - |
dc.date.available | 2016-10-05 | |
dc.date.copyright | 2011-10-05 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-11 | |
dc.identifier.citation | Abbasipour F. et al., (2009). 'The nociceptive and anti-nociceptive effects of white mineral trioxide aggregate.' International Endodontic Journal 42(9): 794-801.
Aeinehchi M. et al., (2003). 'Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report.' International Endodontic Journal 36(3): 225-235. Andelin W. E. et al., (2003). 'Identification of Hard Tissue After Experimental Pulp Capping Using Dentin Sialoprotein (DSP) as a Marker.' Journal of Endodontics 29(10): 646-650. Asgary S. et al., (2005). 'Chemical Differences Between White and Gray Mineral Trioxide Aggregate.' Journal of Endodontics 31(2): 101-103. Asrari M. and Lobner D., (2003). 'In Vitro Neurotoxic Evaluation of Root-end-filling Materials.' Journal of Endodontics 29(11): 743-746. Black L. et al., (2006). 'Hydration of tricalcium aluminate (C3A) in the presence and absence of gypsum-studied by Raman spectroscopy and X-ray diffraction.' Journal of Materials Chemistry 16(13): 1263-1272. Braz M. G. et al., (2006). 'Evaluation of genetic damage in human peripheral lymphocytes exposed to mineral trioxide aggregate and Portland cements.' Journal of Oral Rehabilitation 33(3): 234-239. Butler W. T. et al., (2007). Extracellular Matrix Proteins of Dentine, John Wiley & Sons, Ltd. Camilleri J. (2007). 'Hydration mechanisms of mineral trioxide aggregate.' International Endodontic Journal 40(6): 462-470. Camilleri J. (2009). 'Evaluation of Selected Properties of Mineral Trioxide Aggregate Sealer Cement.' Journal of Endodontics 35(10): 1412-1417. Chen C. L. et al., (2009). 'Comparison of Calcium and Silicate Cement and Mineral Trioxide Aggregate Biologic Effects and Bone Markers Expression in MG63 Cells.' Journal of Endodontics 35(5): 682-685. Cohen, S. and Burns R.C., (1994). Pathways of the pulp. St. Louis, Mosby. Cox C. F. G. et al., (1985). 'Pulp capping of dental pulp mechanically exposed to oral microflora: a 1–2 year observation of wound healing in the monkey.' Journal of Oral Pathology & Medicine 14(2): 156-168. Cox C. F. et al., (1998). 'Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primate teeth.' American Journal of Dentistry 11 Spec No: S55-63. Cox C. F. et al., (1987). 'Biocompatibility of surface-sealed dental materials against exposed pulps.' Journal of Prosthetic Dentistry, The 57(1): 1-8. Cummings G. R. and Torabinejad M., (1995). 'RS 53 Mineral trioxide aggregate (MTA) as an isolating barrier for internal bleaching.' Journal of Endodontics 21(4): 228. Cvek M. (1992). 'Prognosis of luxated non-vital maxillary incisors treated with calcium hydroxide and filled with gutta-percha. A retrospective clinical study.' Dental Traumatology 8(2): 45-55. Cvek M. et al., (1987). 'Hard Tissue Barrier Formation in Pulpotomized Monkey Teeth Capped with Cyanoacrylate or Calcium Hydroxide for 10 and 60 Minutes.' Journal of Dental Research 66(6): 1166-1174. Dammaschke T. et al., (2005). 'Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements.' Dental Materials 21(8): 731-738. Darvell B. W. and Wu R.C.T., (2010). ''MTA'--An Hydraulic Silicate Cement: Review update and setting reaction.' Dental Materials 27(5): 407-422. El Meligy, O. A. S. A. and David R., (2006). 'Comparison of Apexification with Mineral Trioxide Aggregate and Calcium Hydroxide.' Pediatric Dentistry 28: 248-253. Engqvist H. et al., (2005). 'In vivo Bioactivity of a Mineral Based Orthopaedic Biocement.' Trends Biomater. Artif. Organs 19(1): 27-32. Estrela C. et al., (2000). 'Antimicrobial and chemical study of MTA, Portland cement, calcium hydroxide paste, Sealapex and Dycal.' Brazilian Dental Journal 11(1): 3-9. Faraco I. M. and Holland R., (2001). 'Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement.' Dental Traumatology 17(4): 163-166. Ford T. et al., (1996). 'Using mineral trioxide aggregate as a pulp-capping material.' The Journal of the American Dental Association 127(10): 1491-1494. Glickman G. N. and Seale N.S., (2008). 'AAPD and AAE symposium overview: emerging science in pulp therapy--new insights into dilemmas and controversies.' Pediatric Dentistry 30(3): 190-1. Goktas A. A. and Weinberg M.C., (1991). 'Preparation and Crystallization of Sol-Gel Calcia–Alumina Compositions.' Journal of the American Ceramic Society 74(5): 1066-1070. Gorduysus M. et al., (2007). 'Cytotoxic Effects of Four Different Endodontic Materials in Human Periodontal Ligament Fibroblasts.' Journal of Endodontics 33(12): 1450-1454. Gronthos S. et al., (2000). 'Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.' Proceedings of the National Academy of Sciences 97(25): 13625-13630. Guven G. et al., (2007). 'Effect of Mineral Trioxide Aggregate Cements on Transforming Growth Factor [beta]1 and Bone Morphogenetic Protein Production by Human Fibroblasts In Vitro.' Journal of Endodontics 33(4): 447-450. Hafez A. A. et al., (2000). 'Pulpotomy reconsidered: application of an adhesive system to pulpotomized permanent primate pulps.' Quintessence International 31(8): 579-89. Hargreaves K. M. et al., (2002). Seltzer and Bender's dental pulp. Chicago, Quintessence Pub. Co. Hebling J. et al., (1999). 'Biocompatibility of an adhesive system applied to exposed human dental pulp.' Journal of Endodontics 25(10): 676-682. Hench L. L., (1993). 'Introduction to bioceramics.' Adv. Ser. Ceram 1: 1-24. Hench L. L., (1998). 'Bioactive materials: The potential for tissue regeneration.' Journal of Biomedical Materials Research 41(4): 511-518. Hench L. L. and Ethridge E.C., (1982). Biomaterials : an interfacial approach. New York, Academic Press. Hermann, (1930). ' Dentinobliteration der wurzelkanle nach behandlung mit calcium.' Zahnrztliche Rundschau 39: 888-9. Hess W., (1950). 'The treatment of teeth with exposed healthy pulps.' Int. Dent. J. 1: 10-35. Holland R. et al., (1999). 'Reaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggregate or calcium hydroxide.' Journal of Endodontics 25(3): 161-166. Huang T. H. et al., (2005). 'Inflammatory cytokines reaction elicited by root-end filling materials.' Journal of Biomedical Materials Research Part B: Applied Biomaterials 73B(1): 123-128. Inokoshi S. et al., (1982). 'Pulpal Response to a New Adhesive Restorative Resin.' Journal of Dental Research 61(8): 1014-1019. Islam I. et al., (2006). 'Comparison of the Physical and Mechanical Properties of MTA and Portland Cement.' Journal of Endodontics 32(3): 193-197. Jarcho M. et al., (1977). 'Tissue, cellular and subcellular events at a bone-ceramic hydroxylapatite interface.' Journal of Bioengineering 1(2): 79-92. Javelet J. et al., (1985). 'Comparison of two pH levels for the induction of apical barriers in immature teeth of monkeys.' Journal of Endodontics 11(9): 375-378. Jontell M. and Bergenholtz G., (1992). 'Accessory cells in the immune defense of the dental pulp.' Proceedings of the Finnish Dental Society / Suomen Hammaslaakariseuran Toimituksia 88 Suppl 1: 344-55. Kakehashi S. et al., (1965). 'The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats.' Oral Surgery, Oral Medicine, Oral Pathology 20(3): 340-349. Kalitaa S. J. et al., (2002). 'Porous calcium aluminate ceramics for bone-graft applications.' Journal of Materials Research 17(12): 3042-3049. Kao C. T. et al., (2009). 'Properties of an Accelerated Mineral Trioxide Aggregate-like Root-end Filling Material.' Journal of Endodontics 35(2): 239-242. Kao C. T. et al., (2006). 'Tissue and cell reactions to implanted root-end filling materials.' Journal of Materials Science: Materials in Medicine 17(9): 841-847. Kasugai S. et al., (1988). 'Establishment and characterization of a clonal cell line (RPC-C2A) from dental pulp of the rat incisor.' Archives of Oral Biology 33(12): 887-891. Kettering J. D. and Torabinejad M., (1995). 'Investigation of mutagenicity of mineral trioxide aggregate and other commonly used root-end filling materials.' Journal of Endodontics 21(11): 537-539. Kokubo T., (1991). 'Bioactive glass ceramics: properties and applications.' Biomaterials 12(2): 155-163. Kokubo T. et al., (1982). Apatite- and Wollastonite-Containg Glass-Ceramics for Prosthetic Application, Institute for Chemical Research, Kyoto University. Kokubo T. and Takadama H., (2006). 'How useful is SBF in predicting in vivo bone bioactivity?' Biomaterials 27(15): 2907-2915. Lea F. M. and Hewlett P. C., (1998). Lea's chemistry of cement and concrete. New York, Copublished in North, Central, and South America by J. Wiley. Lee S. J. et al., (1993). 'Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations.' Journal of Endodontics 19(11): 541-544. Liu J. et al., (2005). 'In vitro differentiation and mineralization of human dental pulp cells induced by dentin extract.' In Vitro Cellular & Developmental Biology - Animal 41(7): 232-238. Liu W. N. et al., (2010). 'Effect of tricalcium aluminate on the properties of tricalcium silicate–tricalcium aluminate mixtures: setting time, mechanical strength and biocompatibility.' International Endodontic Journal 44(1): 41-50. Min K. S. et al., (2009). 'The Combined Effect of Mineral Trioxide Aggregate and Enamel Matrix Derivative on Odontoblastic Differentiation in Human Dental Pulp Cells.' Journal of Endodontics 35(6): 847-851. Nakashima M., (1994). 'Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix.' Archives of Oral Biology 39(12): 1085-1089. Narasaraju T. S. B. and Phebe D. E., (1996). 'Some physico-chemical aspects of hydroxylapatite.' Journal of Materials Science 31(1): 1-21. Narita H. et al., (2010). 'An explanation of the mineralization mechanism in osteoblasts induced by calcium hydroxide.' Acta Biomaterialia 6(2): 586-590. Oguntebi B. R. et al., (1995). 'Quantitative assessment of dentin bridge formation following pulp-capping in miniature swine.' Journal of Endodontics 21(2): 79-82. Oh S. H. et al., (2004). 'Influence of tricalcium aluminate phase on in vitro biocompatibility and bioactivity of calcium aluminate bone cement.' Journal of materials research 19(4): 1062-1067. Ohtsuki C. et al., (1991). 'Apatite formation on the surface of ceravital-type glass-ceramic in the body.' Journal of Biomedical Materials Research 25(11): 1363-1370. Oliveira I. R. et al., (2010). 'Chemical, physical and mechanical properties of a novel calcium aluminate endodontic cement.' International Endodontic Journal 43(12): 1069-1076. Olmez A. et al., (1998). 'A histopathologic study of direct pulp-capping with adhesive resins.' Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 86(1): 98-103. Orefice R. L. et al., (2001). 'Novel sol-gel bioactive fibers.' Journal of Biomedical Materials Research 55(4): 460-467. Pameijer C. H. and Stanley H. R., (1998). 'The disastrous effects of the 'total etch' technique in vital pulp capping in primates.' American Journal of Dentistry 11 Spec No: S45-54. Parirokh M. et al., (2005). 'A comparative study of white and grey mineral trioxide aggregate as pulp capping agents in dog's teeth.' Dental Traumatology 21(3): 150-154. Ramachandran V. S. and Feldman R. F., (1973). 'Hydration characteristics of monocalcium aluminate at a low water/solid ratio.' Cement and Concrete Research 3(6): 729-750. Rashid F. et al., (2003). 'The Effect of Extracellular Calcium Ion on Gene Expression of Bone-related Proteins in Human Pulp Cells.' Journal of Endodontics 29(2): 104-107. Rejda B. V. et al., (1977). 'Tri-calcium phosphate as a bone substitute.' Journal of Bioengineering 1(2): 93-7. Ribeiro D. A. et al., (2005). 'Biocompatibility In Vitro Tests of Mineral Trioxide Aggregate and Regular and White Portland Cements.' Journal of Endodontics 31(8): 605-607. Ruch J. V., (1998). 'Odontoblast commitment and differentiation.' Biochemistry and Cell Biology 76(6): 923. Rutherford B. and Fitzgerald M., (1995). 'A New Biological Approach To Vital Pulp Therapy.' Critical Reviews in Oral Biology & Medicine 6(3): 218-229. Samachson J., (1969). 'Basic Requirements for Calcification.' Nature 221(5187): 1247-1248. Saravanapavan P. and Hench L. L., (2001). 'Low-temperature synthesis, structure, and bioactivity of gel-derived glasses in the binary CaO-SiO2 system.' Journal of Biomedical Materials Research 54(4): 608-618. Saravanapavan P. et al., (2003). 'Bioactivity of gel–glass powders in the CaO-SiO2 system: A comparison with ternary (CaO-P2P5-SiO2) and quaternary glasses (SiO2-CaO-P2O5-Na2O).' Journal of Biomedical Materials Research Part A 66A(1): 110-119. Sasaki T. and Kawamata-Kido H., (1995). 'Providing an environment for reparative dentine induction in amputated rat molar pulp by high molecular-weight hyaluronic acid.' Archives of Oral Biology 40(3): 209-219. Shabahang S. and Torabinejad M., (2000). 'Treatment of teeth with open apices using mineral trioxide aggregate.' Pract Periodontics Aesthet Dent 12(3): 315-20; quiz 322. Simon S. et al., (2008). 'Evaluation of a new laboratory model for pulp healing: preliminary study.' International Endodontic Journal 41(9): 781-790. Skipper L. J. et al., (2005). 'The structure of a bioactive calcia-silica sol-gel glass.' Journal of Materials Chemistry 15(24): 2369-2374. Sloan A. J. and Smith A. J., (1999). 'Stimulation of the dentine-pulp complex of rat incisor teeth by transforming growth factor-[beta] isoforms 1-3 in vitro.' Archives of Oral Biology 44(2): 149-156. Stanley H. R., (1989). 'Pulp capping: Conserving the dental pulp--Can it be done? Is it worth it?' Oral Surgery, Oral Medicine, Oral Pathology 68(5): 628-639. Stephan D. and Wilhelm P., (2004). 'Synthesis of Pure Cementitious Phases by Sol-Gel Process as Precursor.' Zeitschrift fur anorganische und allgemeine Chemie 630(10): 1477-1483. Sveen O. B. and Hawes R. R., (1968). 'Differentiation of new odontoblasts and dentine bridge formation in rat molar teeth after tooth grinding.' Archives of Oral Biology 13(12): 1399-1410, IN1-IN2, 1411-1412, IN3. Tecles O. et al., (2008). 'Human tooth culture: A study model for reparative dentinogenesis and direct pulp capping materials biocompatibility.' Journal of Biomedical Materials Research Part B: Applied Biomaterials 85B(1): 180-187. Tay F. R. et al., (2007). 'Calcium Phosphate Phase Transformation Produced by the Interaction of the Portland Cement Component of White Mineral Trioxide Aggregate with a Phosphate-containing Fluid.' Journal of Endodontics 33(11): 1347-1351. Taylor H. F. W., (1997). Cement chemistry. London, T. Telford. Thomson T. S. et al., (2003). 'Cementoblasts Maintain Expression of Osteocalcin in the Presence of Mineral Trioxide Aggregate.' Journal of Endodontics 29(6): 407-412. Tomson P. L. et al., (2007). 'Dissolution of bio-active dentine matrix components by mineral trioxide aggregate.' Journal of Dentistry 35(8): 636-642. Torabinejad M. et al., (1995). 'Physical and chemical properties of a new root-end filling material.' Journal of Endodontics 21(7): 349-353. Torabinejad M. et al., (1993). 'Sealing ability of a mineral trioxide aggregate when used as a root end filling material.' Journal of Endodontics 19(12): 591-595. Trope M., (2008). 'Regenerative Potential of Dental Pulp.' Journal of Endodontics 34(7, Supplement 1): S13-S17. Tu Q. et al., (2003). 'Rescue of the skeletal phenotype in CasR-deficient mice by transfer onto the Gcm2 null background.' The Journal of Clinical Investigation 111(7): 1029-1037. Turner D. F. et al., (1989). 'Demonstration of Physiological Barrier Between Pulpal Odontoblasts and its Perturbation Following Routine Restorative Procedures: A Horseradish Peroxidase Tracing Study in the Rat.' Journal of Dental Research 68(8): 1262-1268. Tziafas D. et al., (2002). 'The dentinogenic effect of mineral trioxide aggregate (MTA) in short-term capping experiments.' International Endodontic Journal 35(3): 245-254. Tziafas D. et al., (2000). 'Designing new treatment strategies in vital pulp therapy.' Journal of Dentistry 28(2): 77-92. Viehland D. et al., (1996). 'Mesostructure of Calcium Silicate Hydrate (C-S-H) Gels in Portland Cement Paste: Short-Range Ordering, Nanocrystallinity, and Local Compositional Order.' Journal of the American Ceramic Society 79(7): 1731-1744. Watts A. and Paterson W. C., (1987). 'Pulpal response to a zinc oxide–eugenol cement.' International Endodontic Journal 20(2): 82-86. Weller R. N. et al., (2008). 'Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid.' International Endodontic Journal 41(11): 977-986. White K. C. et al., (1994). 'Pulpal response to adhesive resin systems applied to acid-etched vital dentin: damp versus dry primer application.' Quintessence International 25(4): 259-68. Wlodarski K. and Reddi A., (1986). 'Alkaline phosphatase as a marker of osteoinductive cells.' Calcified Tissue International 39(6): 382-385. Yasuda Y. et al., (2008). 'The Effect of Mineral Trioxide Aggregate on the Mineralization Ability of Rat Dental Pulp Cells: An In Vitro Study.' Journal of Endodontics 34(9): 1057-1060. Yokose S. et al., (2000). 'Establishment and Characterization of a Culture System for Enzymatically Released Rat Dental Pulp Cells.' Calcified Tissue International 66(2): 139-144. Yoshizawa M. et al., (2001). 'Cavity-Directed Synthesis of Labile Silanol Oligomers within Self-Assembled Coordination Cages.' Journal of the American Chemical Society 123(43): 10454-10459. Zander H. A., (1939). 'Reaction of the Pulp to Calcium Hydroxide.' Journal of Dental Research 18(4): 373-379. Zhao W. et al., (2005). 'The self-setting properties and in vitro bioactivity of tricalcium silicate.' Biomaterials 26(31): 6113-6121. 王文熙 (2008). '以溶膠-凝膠法製備介穩水泥並評估其作為牙科逆向封填材料的可能性.' 國立臺灣大學醫學工程學研究所博士論文. 李苑玲 (2006). '鈣矽生醫陶瓷在牙髓病治療之研發與應用.' 國立台灣大學臨床牙醫學研究所博士論文. 郭恬君 (2005). '探討MTA組成成分差異對其材料性質及生物相容性之影響.' 國立台灣大學臨床牙醫學研究所碩士論文. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/37096 | - |
dc.description.abstract | 牙髓為具有修復再生能力的組織,活髓治療目的在治療任何具可回復性的牙髓傷害,將尚未受損的牙髓以合適的材料保護之,以避免任何外來的刺激。目前應用在臨床上成功率最高的活髓材料為MTA,以其有良好的生物相容性、可誘導牙本質再生、對於牙髓組織刺激小等性質,然而MTA的硬化時間長,需二次約診才可完成治療且操作性質差等缺點仍有待改進。
本實驗材料與目前成功率最高的MTA同樣是以C3S/C3A兩種成分為主的鈣矽陶瓷系統,希望藉由調整成分比例(90/10、70/30、50/50)來找出具有適合活髓治療的材料性質與生物特性的組成,並可評估不同比例的C3A對於材料性質、生物活性、生物相容性、增加細胞礦化能力等影響,作為日後發展新材料的依據;而材料的製備也參考本團隊之前所研發的溶膠凝膠法之化學製程,以期製造出更均質、反應性更佳的材料。 實驗主要分為三部分:(一)材料的研發:使用溶膠凝膠技術製備組成比例不同的材料並進行材料性質測試:以X光繞射分析材料結晶相、掃瞄式電子顯微鏡觀察材料表面結構、水合行為、硬化時間、抗壓強度、表面微硬度等;(二)體外生物活性指標測試;(三)生物相容性及鈣化能力:以MTT assay進行細胞毒性測試,以ALP assay及Alizarin test評估細胞鈣化能力。 C3A比例的增加有效縮短材料的硬化時間,但70/30、50/50的組別同時會減低材料機械強度,然而早期(一天)的表面微硬度則會上升,10%的組別則與市售MTA無顯著差異。所有材料組別皆有生物活性、鹼性磷酸酶活性及礦化小體的生成,然而表現上組別90/10與市售MTA優於組別50/50與70/30。此外,組別90/10除了與市售MTA擁有同樣優異的生物表現之外,以溶膠凝膠法製備更讓此材料具較高的反應性,初硬化時間顯著較市售MTA減少,所以作為活髓治療材料具相當潛力。 | zh_TW |
dc.description.abstract | In dentistry, the goal of vital pulp therapy involves removing diseased pulp tissue and covering the remaining undamaged tissue with appropriate materials to induce pulp
regeneration. Although plausible clinical results could often be achieved, the actual mechanism of pulp-dentin repair is still unknown. New materials need to be developed for reliable prognosis. The purpose of this study was to examine the physico-chemical properties and biological performances of novel calcium silicate biomaterials prepared from mixing different ratio of tricalcium silicate(C3S) and tricalcium aluminate(C3A) which aimed to improve the setting time which was compared with that of ProRoot white MTA without any change in biological properties. This study contained three experimental groups which contain C3S and C3A with different ratio (C3S/C3A: 90/10, 70/30, 50/50) and were fabricated with sol-gel technique; white-colored ProRoot MTA (Dentsply) was comparable group. The setting times, micro-hardness value, morphology and phase composition of hydration products and ex vivo bioactivity were evaluated, as well as the biocompatibility and mineralization, which use of MTT assay to evaluate cytotoxicity and use of ALP assay and Alizarin test to evaluate mineralization. As solid phases determined by XRD, the material powder with different C3A content were proved. The initial setting times of 70/30, 50/50 groups were in the range of 10-25 minutes, which are significantly (p<0.05, ANOVA and post-hoc test) lower than those obtained for white-colored ProRoot MTA (165 minutes) and 90/10 group(81 minutes). The micro-hardness of 50/50 and 70/30 were also significantly(p<0.05) higher than ProRoot MTA in the first day. All groups demonstrated ex vivo bioactivity when they came into contact with phosphate ions. The biocompatibility results of all groups were as good as the negative control except 50/50 with mild cytotoxicity. Results of mineralization test showed that all groups could induce hard tissue formation. Then we concluded in this study that, first, 90/10 group has optimal compressive strength, biocompatibility, and mineralization ability. Moreover, its material property is more reactive than white MTA. Second, with C3A increased to 30% to 50% in calcium silicate ceramics significantly improved setting time and early microhardness of the material, however, also exacerbating its material strength and biological properties. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T15:19:08Z (GMT). No. of bitstreams: 1 ntu-100-R97422020-1.pdf: 13131461 bytes, checksum: d18d2a0f047ad947f9e92a497a22fdd9 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 中文摘要 ----------------------------------------------------------------------------------------- i
英文摘要 ----------------------------------------------------------------------------------------- ii 目錄 ----------------------------------------------------------------------------------------------- iv 圖次 ---------------------------------------------------------------------------------------------- viii 表次 ----------------------------------------------------------------------------------------------- ix 縮寫表 -------------------------------------------------------------------------------------------- x 第一章 前言 ------------------------------------------------------------------------------------ 1 第二章 文獻回顧 -------------------------------------------------------------------------------2 2.1 保存牙髓之重要性 ----------------------------------------------------------------- 2 2.1.1 牙髓之構造與功能 --------------------------------------------------------- 2 2.1.2 保留牙髓於臨床治療的意義 --------------------------------------------- 2 2.2 活髓治療 ----------------------------------------------------------------------------- 3 2.2.1 活髓治療之介紹 ------------------------------------------------------------ 3 2.2.2 理想活髓治療材料之需求 ------------------------------------------------ 4 2.2.3 活髓材料之發展沿革 ------------------------------------------------------ 5 2.2.3.1 氫氧化鈣 ------------------------------------------------------------- 5 2.2.3.2 複合樹脂類 ---------------------------------------------------------- 6 2.2.3.3 Mineral Trioxide Aggregate ----------------------------------------- 7 2.3 鈣矽生醫陶瓷 ----------------------------------------------------------------------- 9 2.4 本研究團隊研發之鈣矽生醫陶瓷 ---------------------------------------------- 10 2.5 鋁酸三鈣(C3A)於鈣矽陶瓷系統之作用 -------------------------------------- 11 2.6 活髓治療材料之研究與評估 ---------------------------------------------------- 12 2.6.1 MTT 測試 ------------------------------------------------------------------- 12 2.6.2 生物活性之評估 ----------------------------------------------------------- 13 2.6.3 鹼性磷酸酶與礦化之關係 ----------------------------------------------- 14 第三章 動機與目的 -------------------------------------------------------------------------- 15 第四章 材料與方法 -------------------------------------------------------------------------- 17 4.1實驗材料之製備與評估 ----------------------------------------------------------- 17 4.1.1材料製備 --------------------------------------------------------------------- 17 4.1.2 掃瞄式電子顯微鏡觀察材料粉末 -------------------------------------- 17 4.1.3 X光繞射分析評估材料粉末 --------------------------------------------- 18 4.2水合產物分析 ----------------------------------------------------------------------- 18 4.2.1測試樣本製備 --------------------------------------------------------------- 18 4.2.2掃瞄式電子顯微鏡進行樣本觀察 --------------------------------------- 19 4.2.3 X光繞射分析評估樣本結晶相 ------------------------------------------ 19 4.3硬化時間測試 ----------------------------------------------------------------------- 19 4.3.1測試樣本製備 --------------------------------------------------------------- 19 4.3.2測試方法 --------------------------------------------------------------------- 19 4.3.3資料分析 --------------------------------------------------------------------- 20 4.4表面微硬度測試(Vicker’s hardness test) ----------------------------------------- 20 4.4.1 測試樣本製備 -------------------------------------------------------------- 20 4.4.2 微硬度儀器規格 ----------------------------------------------------------- 20 4.4.3測試方法 --------------------------------------------------------------------- 20 4.4.4 資料分析 -------------------------------------------------------------------- 21 4.5抗壓強度測試 ----------------------------------------------------------------------- 21 4.5.1測試樣本製備 --------------------------------------------------------------- 21 4.5.2抗壓強度測試 --------------------------------------------------------------- 21 4.5.3實驗過程及記錄 ------------------------------------------------------------ 21 4.5.4結果分析 --------------------------------------------------------------------- 22 4.6體外生物活性測試 ----------------------------------------------------------------- 22 4.6.1模擬體液溶液之製備 ------------------------------------------------------ 23 4.6.2測試樣本製備 --------------------------------------------------------------- 23 4.6.3掃瞄式電子顯微鏡 --------------------------------------------------------- 23 4.6.4 X光繞射分析 --------------------------------------------------------------- 23 4.6.5 SBF溶液酸鹼值變化觀察 ------------------------------------------------ 23 4.7材料生物相容性評估 --------------------------------------------------------------- 23 4.7.1 3T3細胞株培養 ------------------------------------------------------------- 23 4.7.1.1解凍細胞 ------------------------------------------------------------- 24 4.7.1.2繼代培養 ------------------------------------------------------------- 24 4.7.1.3細胞計數 ------------------------------------------------------------- 24 4.7.2人類牙髓細胞之初級培養 ------------------------------------------------ 25 4.7.3材料萃取液之製備 --------------------------------------------------------- 25 4.7.4 MTT測試 -------------------------------------------------------------------- 25 4.7.4.1 MTT dye配置 ------------------------------------------------------- 26 4.7.4.2實驗步驟 ------------------------------------------------------------- 26 4.8鹼性磷酸酶定性染色分析 -------------------------------------------------------- 26 4.8.1 Incubation soluteion配置 ---------------- --------------------------------- 26 4.8.2含鈣、鎂之PBS配置 ----------------------------------------------------- 27 4.8.3實驗步驟 --------------------------------------------------------------------- 27 4.9細胞基質礦化小體染色(Alizarin Red S staining) ------------------------------ 27 第五章 實驗結果 ------------------------------------------------------------------------------ 29 5.1材料粉末性質分析 ------------------------------------------------------------------ 29 5.1.1 材料粉末之X光繞射分析 ----------------------------------------------- 29 5.1.2材料粉末之掃瞄式電子顯微鏡觀察 ------------------------------------- 29 5.2水合產物性質分析 ------------------------------------------------------------------ 29 5.2.1 水合產物之X光繞射分析 ----------------------------------------------- 29 5.2.2水合產物掃瞄式電子顯微鏡觀察 --------------------------------------- 30 5.3硬化時間測試 ----------------------------------------------------------------------- 30 5.4表面微硬度測試 -------------------------------------------------------------------- 31 5.5抗壓強度測試 ----------------------------------------------------------------------- 31 5.6體外生物活性測試 ----------------------------------------------------------------- 32 5.6.1 X光繞射分析 --------------------------------------------------------------- 32 5.6.2掃瞄式電子顯微鏡觀察 --------------------------------------------------- 33 5.6.3 SBF溶液酸鹼值測試 ------------------------------------------------------ 33 5.7體外細胞毒性MTT測試 --------------------------------------------------------- 33 5.8鹼性磷酸酶定性染色分析 -------------------------------------------------------- 34 5.9細胞基質礦化小體染色分析 ----------------------------------------------------- 34 第六章 討論 ----------------------------------------------------------------------------------- 35 6.1本實驗材料與MTA之組成、水合行為及pH值比較 ---------------------- 35 6.2探討C3A對於鈣矽材料硬化時間、表面硬度與抗壓強度的影響 ------- 37 6.3探討C3A含量對於鈣矽材料生物活性的影響 ------------------------------- 39 6.4探討C3A對於鈣矽材料生物相容性及礦化能力的影響 ------------------- 41 第七章 結論與未來研究方向 -------------------------------------------------------------- 45 參考文獻 ---------------------------------------------------------------------------------------- 47 | |
dc.language.iso | zh-TW | |
dc.title | 三鈣鋁酸含量對鈣矽陶瓷的材料性質及生物特性之影響 | zh_TW |
dc.title | Effect of tricalcium aluminate on material properties, bioactivity and biocompatibility of calcium silicate ceramics | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 林峰輝(Feng-Huei Lin) | |
dc.contributor.oralexamcommittee | 姜昱至(Yu-Chih Chiang) | |
dc.subject.keyword | 鋁酸三鈣,鈣矽陶瓷,活髓治療溶膠-凝膠法,礦化,生物相容性, | zh_TW |
dc.subject.keyword | Tricalcium aluminate,Calcium silicate ceramics,Vital pulp therapy,Sol-gel method,Mineralization,Biocompatibility, | en |
dc.relation.page | 90 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-11 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 12.82 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。