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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖一久(I-Chiu Liao),羅竹芳(Chu-Fang Lo) | |
| dc.contributor.author | Shih-Ting Kang | en |
| dc.contributor.author | 康詩婷 | zh_TW |
| dc.date.accessioned | 2021-06-13T08:09:44Z | - |
| dc.date.available | 2006-07-26 | |
| dc.date.copyright | 2005-07-26 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-20 | |
| dc.identifier.citation | Aranda M, Maule A (1998) Virus-induced host gene shutoff in animals and plants. Virology 243:261-267
Bieleski L, Hindley C, Talbot SJ (2004) A polypyrimidine tract facilitates the expression of Kaposi's sarcoma-associated herpesvirus vFLIP through an internal ribosome entry site. J Gen Virol 85:615-620 Bieleski L, Talbot SJ (2001) Kaposi's sarcoma-associated herpesvirus vCyclin open reading frame contains an internal ribosome entry site. J Virol 75:1864-1869 Cao J, Geballe AP (1994) Mutational analysis of the translational signal in the human cytomegalovirus gpUL4 (gp48) transcript leader by retroviral infection. Virology 205:151-160 Cao J, Geballe AP (1998) Ribosomal release without peptidyl tRNA hydrolysis at translation termination in a eukaryotic system. Rna 4:181-188 Chang PS, Lo CF, Wang YC, Kou GH (1996) Identification of white spot syndrome associated baculovirus (WSSV) target organs in shrimp, Penaeus monodon, by in situ hybridization. Dis Aquat Org 27:131-139 Chang YS, Peng SE, Wang HC, Hsu HC, Ho CH, Wang CH, Wang SY, Lo CF, Kou GH (2001) Sequencing and amplified restriction fragment length polymorphism analysis of ribonucleotide reductase large subunit gene of the white spot syndrome virus in blue crab (Callinectes sapidus) from American Coastal Waters. Mar Biotechnol (NY) 3:163-171 Chen LL, Leu JH, Huang CJ, Chou CM, Chen SM, Wang CH, Lo CF, Kou GH (2002a) Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting VP35 to the nuclei of transfected insect cells. Virology 293:44-53 Chen LL, Lo CF, Chiu YL, Chang CF, Kou GH (2000) Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata. Dis Aquat Org 40:157-161 Chen LL, Wang HC, Huang CJ, Peng SE, Chen YG, Lin SJ, Chen WY, Dai CF, Yu HT, Wang CH, Lo CF, Kou GH (2002b) Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus. Virology 301:136-147 Chou HY, Huang CY, Wang CH, Kou GH, Lo CF (1995) Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis Aquat Org 23:165-173 Cornelis S, Bruynooghe Y, Denecker G, Van Huffel S, Tinton S, Beyaert R (2000) Identification and characterization of a novel cell cycle-regulated internal ribosome entry site. Mol Cell 5:597-605 Crum CJ, Hu J, Hiddinga HJ, Roth DA (1988) Tobacco mosaic virus infection stimulates the phosphorylation of a plant protein associated with double-stranded RNA-dependent protein kinase activity. J Biol Chem 263:13440-13443 Felsenstein KM, Goff SP (1988) Expression of the gag-pol fusion protein of Moloney murine leukemia virus without gag protein does not induce virion formation or proteolytic processing. J Virol 62:2179-2182 Felsenstein KM, Goff SP (1992) Mutational analysis of the gag-pol junction of Moloney murine leukemia virus: requirements for expression of the gag-pol fusion protein. J Virol 66:6601-6608 Fields BN (1990) Virology. Raven Press, New York, N.Y. Flegel TW (1997) Special topic review: major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World J Microbiol and Biotechnol 13:433 ~ 442 Gale M, Jr., Tan SL, Katze MG (2000) Translational control of viral gene expression in eukaryotes. Microbiol Mol Biol Rev 64:239-280 Geballe AP, Mocarski ES (1988) Translational control of cytomegalovirus gene expression is mediated by upstream AUG codons. J Virol 62:3334-3340 Gesteland RF, Atkins JF (1996) Recoding: dynamic reprogramming of translation. Annu Rev Biochem 65:741-768 Gingras AC, Svitkin Y, Belsham GJ, Pause A, Sonenberg N (1996) Activation of the translational suppressor 4E-BP1 following infection with encephalomyocarditis virus and poliovirus. Proc Natl Acad Sci U S A 93:5578-5583 Glass MJ, Summers DF (1992) A cis-acting element within the hepatitis A virus 5'-non-coding region required for in vitro translation. Virus Res 26:15-31 Haghighat A, Sonenberg N (1997) eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-cap structure. J Biol Chem 272:21677-21680 Hasson KW, Lightner DV, Poulos BT, Redman RM, White BL, Brock JA (1995) Taura syndrome in Penaeus vannamei: demonstration of a viral etiology. Dis Aquat Org 23:115-126 Hellen CU, Wimmer E (1995) Translation of encephalomyocarditis virus RNA by internal ribosomal entry. Curr Top Microbiol Immunol 203:31-63 Holcik M, Korneluk RG (2000) Functional characterization of the X-linked inhibitor of apoptosis (XIAP) internal ribosome entry site element: role of La autoantigen in XIAP translation. Mol Cell Biol 20:4648-4657 Huang J, Song XL, Yu J, Yang CH (1995a) Baculoviral hypodermal and hematopoietic necrosis - study on the pathogen and pathology of the explosive epidemic disease of shrimp. Mar Fish Res 16:1 ~ 10 Huang J, Yu J, Song XL, Kong J, Yang CH (1995b) Studies on fine structure, nucleic acid, polypeptide and serology of hypodermal and hematopoietic necrosis baculovirus of penaeid shrimp. Mar Fish Res 16:11 ~ 23 Inouye K, Miwa S, Oseko N, Nakano H, Kimura T, Momoyama K, Hiraoka M (1994) Mass mortalities of cultured kuruma shrimp Penaeus japonicus in Japan in 1993: Electron microscopic evidence of the causative virus. Fish Pathol 29:149 ~ 158 Inouye K, Yamano K, Ikeda N, Kimura T, Nakano H, Momoyama K, Kobayashi J, Miyajima S (1996) The penaeid rod-shaped DNA virus (PRDV), which causes penaeid acute viremia (PAV). Fish Pathol 31:39 ~ 45 Jackson RJ, Kaminski A (1995) Internal initiation of translation in eukaryotes: the picornavirus paradigm and beyond. Rna 1:985-1000 Jang SK, Davies MV, Kaufman RJ, Wimmer E (1989) Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo. J Virol 63:1651-1660 Jang SK, Krausslich HG, Nicklin MJ, Duke GM, Palmenberg AC, Wimmer E (1988) A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J Virol 62:2636-2643 Karunasagar I, Otta SK, Karunasagar I (1997) Histopathological and bacteriological study of white spot syndrome of Penaeus monodon along the west coast of India. Aquaculture 153:9 ~ 13 Karunasagar I, Otta SK, Karunasagar I (1998) Disease problems affecting cultured penaeid shrimp in India. Fish Pathol 33:413 ~ 419 Kozak M (1978) How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell 15:1109-1123 Kozak M (1987) Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol 7:3438-3445 Kozak M (1989) The scanning model for translation: an update. J Cell Biol 108:229-241 Leu JH, Tsai JM, Wang HC, Wang AH, Wang CH, Kou GH, Lo CF (2005) The unique stacked rings in the nucleocapsid of the white spot syndrome virus virion are formed by the major structural protein VP664, the largest viral structural protein ever found. J Virol 79:140-149 Liao IC (1989a) Penaeus monodon culture in Taiwan: Through two decades of growth. Int J Aquacult Fish Technol 1:16-24 Liao IC (1989b) Taiwanese shrimp culture: A molting industry. In: Chauvun K, Menesses P, Chauvin W, Cuccia A (eds) The Fourth Shrimp World Marketing Conference, New Orleans, Louisiana, U.S.A., p 55-83 Liao IC, Huang TL, Katsutani K (1969) A preliminary report on artificial propagation of Penaeus monodon (Fabricius). Jt. Comm. Rural Reconstr. Fish. Ser. 8:67-71 Lightner DV (1996) A handbook of shrimp pathology and diagnostic procedures for diseases of penaeid shrimp World Aquaculture Society, Baton Rouge, Louisiana Lightner DV, Poulos BT, Redman RM, Mari J, Bonami JR (1992) New developmentsin penaeid virology: Application of biotechnology in research and disease diagnosisfor shrimp viruses of concern in the Americas. In: Fulks W, Main K (eds) Proceedings of the Asian Interchange Program Workshop on the Diseases of Cultured Penaeid Shrimp, Asian Interchange Program, The Oceanic Institute, Oahu,HI, p 233-253 Liu WJ, Yu HT, Peng SE, Chang YS, Pien HW, Lin CJ, Huang CJ, Tsai MF, Huang CJ, Wang CH, Lin JY, Lo CF, Kou GH (2001) Cloning, characterization, and phylogenetic analysis of a shrimp white spot syndrome virus gene that encodes a protein kinase. Virology 289:362-377 Lo CF, Ho CH, Chen CH, Liu KF, Chiu YL, Yeh PY, Peng SE, Hsu HC, Liu HC, Chang CF, Su MS, Wang CH, Kou GH (1997) Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis Aquat Org 30: 53 ~ 72 Lo CF, Ho CH, Peng SE, Chen CH, Hsu HC, Chiu YL, Chen YT, Chang CF, Liu KF, Su MS, Wang CH, Kou GH (1996) Infection of white spot syndrome associated virus (WSBV) in cultured and wild-caught shrimps, crabs and other arthropods. Dis Aquat Org 27:215-225 Lo CF, Kou GH (1998) Virus-associated white spot syndrome of shrimp in Taiwan: a review. Fish Pathol 33:365 ~ 371 Loh PC, Cesar E, Nadala Jr B, Tapay LM, Lu Y (1998) Recent developments in immunologically-based and cell culture protocols for the specific detection of shrimp viral pathogens. In: Flegel TW (ed) Advances in shrimp biotechnology,National Center for Genetic Engineering and Biotechnology, Bangkok, p 255 ~ 259 Lotz JM, Browdy CL, Carr WH, Frelier PF, Lightner DV (1995) USMSFP suggested procedures and guidelines for assuring the specific pathogen status of shrimp broodstock and seed. In: Browdy CL, Hopkins JS (eds) Swimming Through Troubled Water, Proceedings the Special Session on Shrimp Farming, Aquaculture '95. World Aquaculture Society, Baton Rouge, Louisina, USA, p 66 ~ 75 Low W, Harries M, Ye H, Du MQ, Boshoff C, Collins M (2001) Internal ribosome entry site regulates translation of Kaposi's sarcoma-associated herpesvirus FLICE inhibitory protein. J Virol 75:2938-2945 Lu CP, Zhu S, Guo FS, Wu SY (1997) Electron microscopic observation on a non-occluded baculo-like virus in shrimps. Arch Virol 142:2073-2078 Magbanua FO, Natividad KT, Migo VP, Alfafara CG, de la Pena FO, Miranda RO, Albaladejo JD, Nadala EC, Jr., Loh PC, Mahilum-Tapay L (2000) White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines. Dis Aquat Org 42:77-82 Marks H, Mennens M, Vlak JM, van Hulten MC (2003) Transcriptional analysis of the white spot syndrome virus major virion protein genes. J Gen Virol 84:1517-1523 Matthews M (1996) Interactions between viruses and the cellular machinery for protein synthesis. In: Hershey J, Mathews M, Sonenberg N (eds) Translational control. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., p 505-548 Merrick WC, Hershey JWB (1996) The pathway and mechanism of eukaryotic protein synthesis. In: B HJW, B. MM, N. S (eds) Translational control. Cold Spring Harbor Laboratory Press, Plainview, N.Y., p 31-70 Mohan CV, Shankar KM, Kulkarni S, Sudha PM (1998) Histopathology of cultured shrimp showing gross signs of yellow head syndrome and white spot syndrome during 1994 Indian epizootics. Dis Aquat Org 34:9-12 Momoyama K, Hiraoka M, Nakano H, Koube H, Inouye aK, Oseko N (1994) Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Histopathological study. Fish Pathol 29:141-148 Moon CH, Do JW, Cha SJ, Yoon WJ, Kim SB, Ko MS, Park MA, Kim JW, Sohn SK, Lee JH, Park JW (2003) Highly conserved sequences of three major virion proteins of a Korean isolate of white spot syndrome virus (WSSV). Dis Aquat Organ 53:11-13 Nadala ECB, Tappy LM, Loh PC (1997) Yellow-head virus: a rhabdovirus-like pathogen of penaeid shrimp. Dis Aquat Org 31:141–146 Nakano H, Koube H, Umezawa K, Momoyama K, Hiraoka M, Inouye K, Oseko N (1994) Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Epizootiological survey and infection trials. Fish Pathol 29:135-139 Nanbru C, Lafon I, Audigier S, Gensac MC, Vagner S, Huez G, Prats AC (1997) Alternative translation of the proto-oncogene c-myc by an internal ribosome entry site. J Biol Chem 272:32061-32066 Park JH, Lee YS, Lee S, Lee Y (1998) An infectious viral disease of penaeid shrimp newly found in Korea. Dis Aquat Org 34:71 ~ 75 Pelletier J, Sonenberg N (1988) Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature 334:320-325 Pestova TV, Borukhov SI, Hellen CU (1998) Eukaryotic ribosomes require initiation factors 1 and 1A to locate initiation codons. Nature 394:854-859 Pestova TV, Hellen CU (2000) The structure and function of initiation factors in eukaryotic protein synthesis. Cell Mol Life Sci 57:651-674 Reynolds JE, Kaminski A, Carroll AR, Clarke BE, Rowlands DJ, Jackson RJ (1996) Internal initiation of translation of hepatitis C virus RNA: the ribosome entry site is at the authentic initiation codon. Rna 2:867-878 Rosenberry B, editor (1996) World shrimp Farming 1996 Shrimp News International, San Diego, p 164 Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn, Vol. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Sangamaheswaran AP, Jeyaseelan MJP (2001) White spot viral disease in penaeid shrimp – a review. The ICLARM Quarterly 24:16 ~ 22 Seok SH, Park JH, Cho SA, Baek MW, Lee HY, Kim DJ, Park JH (2004) Cloning and sequencing of envelope proteins (VP19, VP28) and nucleocapsid proteins (VP15, VP35) of a white spot syndrome virus isolate from Korean shrimp. Dis Aquat Organ 60:85-88 Soto MA, Lotz JM (2001) Epidemiological parameters of White Spot Syndrome Virus infections in Litopenaeus vannamei and L. setiferus. J Invertebr Pathol 78:9-15 Stein I, Itin A, Einat P, Skaliter R, Grossman Z, Keshet E (1998) Translation of vascular endothelial growth factor mRNA by internal ribosome entry: implications for translation under hypoxia. Mol Cell Biol 18:3112-3119 Talbot SJ, Weiss RA, Kellam P, Boshoff C (1999) Transcriptional analysis of human herpesvirus-8 open reading frames 71, 72, 73, K14, and 74 in a primary effusion lymphoma cell line. Virology 257:84-94 Tsai JM, Wang HC, Leu JH, Hsiao HH, Wang AH, Kou GH, Lo CF (2004) Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol 78:11360-11370 Tsai MF, Lo CF, van Hulten MC, Tzeng HF, Chou CM, Huang CJ, Wang CH, Lin JY, Vlak JM, Kou GH (2000a) Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus. Virology 277:92-99 Tsai MF, Yu HT, Tzeng HF, Leu JH, Chou CM, Huang CJ, Wang CH, Lin JY, Kou GH, Lo CF (2000b) Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology 277:100-110 Van Etten JL, Graves MV, Muller DG, Boland W, Delaroque N (2002) Phycodnaviridae--large DNA algal viruses. Arch Virol 147:1479-1516 van Hulten MC, Reijns M, Vermeesch AM, Zandbergen F, Vlak JM (2002) Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. J Gen Virol 83:257-265 van Hulten MC, Vlak JM (2001) Identification and phylogeny of a protein kinase gene of white spot syndrome virus. Virus Genes 22:201-207 Wang CH, Lo CF, Leu JH, Chou CM, Yeh PY, Chou HY, E. TM, Chang CF, Su MS, Kou GH (1995) Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis Aquat Org 23:239-242 Wang FZ, Akula SM, Sharma-Walia N, Zeng L, Chandran B (2003) Human herpesvirus 8 envelope glycoprotein B mediates cell adhesion via its RGD sequence. J Virol 77:3131-3147 Wang Q, Nunan LM, Lightner DV (2000) Identification of genomic variations among geographic isolates of white spot syndrome virus using restriction analysis and Southern blot hybridization. Dis Aquat Org 43:175-181 Wongteerasupaya C, Vickers JE, Sriurairatana S, Nash GL, Akarajamorn A, Boonsaeng V, Panyim S, Tassankajon A, Withyanchumnarnkul B, Flegel TW (1995) A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in black tiger prawn Penaeus monodon. Dis Aquat Org 21:69-77 Wyckoff EE (1993) Inhibition of host cell protein synthesis in poliovirus- infected cells. Semin Virol 4:209-215 Yan DC, Dong SL, Huang J, Yu XM, Feng MY, Liu XY (2004) White spot syndrome virus (WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp pond sediments. Dis Aquat Organ 59:69-73 Yang F, He J, Lin X, Li Q, Pan D, Zhang X, Xu X (2001) Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75:11811-11820 Yi G, Wang Z, Qi Y, Yao L, Qian J, Hu L (2004) Vp28 of shrimp white spot syndrome virus is involved in the attachment and penetration into shrimp cells. J Biochem Mol Biol 37:726-734 Yoganandhan K, Syed Musthaq S, Narayanan RB, Sahul Hameed AS (2004) Production of polyclonal antiserum against recombinant VP28 protein and its application for the detection of white spot syndrome virus in crustaceans. J Fish Dis 27:517-522 Yoshinaka Y, Katoh I, Copeland TD, Oroszlan S (1985) Translational readthrough of an amber termination codon during synthesis of feline leukemia virus protease. J Virol 55:870-873 Zhouravleva G, Frolova L, Le Goff X, Le Guellec R, Inge-Vechtomov S, Kisselev L, Philippe M (1995) Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3. Embo J 14:4065-4072 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36656 | - |
| dc.description.abstract | 由蝦類白點症病毒 (white spot syndrome virus, WSSV) 引起之白點症為目前嚴重威脅全世界養蝦產業之病毒性疾病。基於白點症病毒蛋白質體學及基因體序列分析之研究，在其532個預測之開放譯讀區中，有部分功能已知之非結構性蛋白質及結構性蛋白質基因，在其轉譯終點前後不具有聚腺嘌呤訊號 (AATAAA)，推測可能與其下游之開放譯讀區共用加聚腺嘌呤位置 (poly A addition site) 有關。白點症病毒結構性蛋白質基因wssv396、wssv395及wssv394為同向轉錄基因群 (cluster)，三者之加聚腺嘌呤位置皆位於wssv394基因下游之聚腺嘌呤訊號後第17個核苷酸處，符合真核細胞聚腺嘌呤訊號規則。根據北方雜合法分析結果，此基因群利用wssv396之5’端啟動子 (promoter) 轉錄出包含wssv396、wssv395及wssv394之三基因mRNA (3.4 kb)；wssv394基因之riboprobe尚可偵測到利用其5’端啟動子轉錄之單基因mRNA (1.6 kb)。實驗結果證明wssv396及wssv394基因主要利用帽依賴型轉譯作用 (cap-dependent translation) 合成蛋白質，而位於第二個基因之wssv395係以非帽依賴型轉譯作用即內部核醣體進入位置 (Internal Ribosome Entry Site, IRES)合成蛋白質，推測IRES序列可能位於wssv396基因轉錄區內 (coding region)，關於其確切位置猶待進一步之實驗分析以確認之。 | zh_TW |
| dc.description.abstract | WSSV396, WSSV395 and WSSV394 are identified in pervious research as the three of thirty-nine structural proteins of shrimp white spot syndrome virus (WSSV). This study focuses on the transcriptional and translational analysis of wssv396, wssv395 and wssv394. The 3’RACE results reveal that wssv396, wssv395, and wssv394 share the same polyA tail. A common ~3.4-kb transcript, which is consistent with the predicted size of a polycistronic transcript encoding these three genes, was detected by using gene-specific probes respectively in northern blot analysis. Two primer sets were designed to generate two overlapping RT-PCR products to confirm the existence of the ~3.4-kb transcript, and the sequence data also indicated that the ~3.4-kb transcript is entire and intron-less. It reveals that wssv396, wssv395 and wssv394 are transcribed from the same promoter of wssv396. In the northern blot analysis, another ~1.6-kb transcript was recognized by wssv394-specific probe. According to the in vitro transcription and translation data, it could be concluded that WSSV396 and WSSV394 are translated from the ~3.4-kb and ~1.6-kb transcript by cap-dependent translation mechanism; the wssv395 is translated from the ~3.4-kb transcript by cap-independent translational mechanism. Based on the in vitro transcription and translation analysis of bicistronic constructs encoding wssv396 and wssv395, it suggests that the translational mechanism of wssv395 from the polycistronic transcript might occur by a mechanism using the internal ribosome entry site (IRES) on the upstream of the wssv396 ORF. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T08:09:44Z (GMT). No. of bitstreams: 1 ntu-94-R92b41016-1.pdf: 1857140 bytes, checksum: 464d8fd8c13a4ebc218c2f263cdd2e09 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 前言...............................................1
材料與方法................................................................................................................14 1. 白點症病毒液之製備及人工注射感染試驗..................................................14 2. 純化及製備白點症病毒顆粒..........................................................................15 3. 蝦體組織全蛋白之萃取..................................................................................16 4. 檢體RNA之萃取...........................................................................................16 5. 反轉錄酶-聚合酶鏈反應................................................................................16 6. 快速增殖cDNA末端.....................................................................................18 7. 北方轉印雜合法..............................................................................................20 8. 大腸桿菌基因表現..........................................................................................24 9. 大腸桿菌之轉型作用......................................................................................26 10. 西方轉印法......................................................................................................26 11. 以RT-PCR分析wssv396/wssv395/wssv394三基因mRNA.........................27 12. 構築wssv396/wssv395/wssv394三基因質體.................................................28 13. 構築wssv396/wssv395雙基因質體................................................................29 14. 試管內轉錄和轉譯反應..................................................................................31 結果............................................................................................................................33 1. 白點症病毒基因wssv396.................................................................................33 2. 白點症病毒wssv396基因轉錄表現................................................................34 3. 結構性蛋白質WSSV396於白點症病毒顆粒上之位置................................34 4. 結構性蛋白質WSSV396轉譯時序點之分析.................................................35 5. 白點症病毒wssv396基因5’端起始點及wssv396、wssv395基因3’端加聚腺 嘌呤位置分析.........................................................................................................36 6. 白點症病毒wssv396基因轉錄本分析............................................................36 7. 白點症病毒wssv395基因轉錄本分析............................................................37 8.白點症病毒wssv394基因轉錄本分析..............................................................37 9. 分析wssv396/wssv395/wssv394三基因mRNA之剪接作用........................38 10. 探討wssv396、wssv395及wssv394基因轉錄及轉譯作用之相關性........38 討論.............................................................................................................................42 結論.............................................................................................................................51 未來研究方向.............................................................................................................52 參考文獻........................................................................ ............................................53 圖表及附錄.................................................................................................................64 | |
| dc.language.iso | zh-TW | |
| dc.title | 蝦白點症病毒結構性蛋白質基因wssv396轉錄及轉譯機制之分析 | zh_TW |
| dc.title | Transcriptional and translational analysis of shrimp white spot syndrome virus structural protein gene wssv396 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 郭光雄,王重雄,陳歷歷 | |
| dc.subject.keyword | 白點症病毒,結構性蛋白質,蝦, | zh_TW |
| dc.subject.keyword | wssv,structural protein,shrimp, | en |
| dc.relation.page | 80 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-21 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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