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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 盧虎生 | |
dc.contributor.author | Hsiang-Yi Wang | en |
dc.contributor.author | 王湘儀 | zh_TW |
dc.date.accessioned | 2021-06-13T08:06:05Z | - |
dc.date.available | 2006-07-26 | |
dc.date.copyright | 2005-07-26 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-21 | |
dc.identifier.citation | 宋勳、劉瑋婷。 1996。稻米品質的影響因素與分級。稻作生產改進策略研討會專刊。59:133-152。
宋勳、洪梅珠、許愛娜。1991。台灣稻米品質之研究。台中區農業改良場特刊。24:1-43。 林韶凱。2000。充實期溫度對水稻穀粒蛋白質表現之影響。國立台灣大學農藝學系碩士論文:pp3-11。 洪梅珠、簡珮如、盧虎生。2000。米飯食味特性與白米醇溶性及鹼溶性蛋白質間相關之研究。台中區農業改良場研究彙報。67:1-10。 莊榮輝。2001。表現蛋白質之純化與檢定。生物技術方法 生物技術核心實驗。卷一:84-86。國立台灣大學。 楊蕓瑋。2003。低溫及離層酸處理下水稻幼苗根部之蛋白質體學分 析。國立台灣大學農藝學系碩士論文:pp14。 鄭心嫻、張為憲。1995。以不同溶劑依序抽取米蛋白質區分條件之探討。中國農業化學會誌。33:570-578。 盧虎生。2004。水稻之發育過程與健康管理。水稻健康管理研討會刊。pp17-32。財團法人全方位農業振興基金會。 簡珮如、盧虎生、朱鈞。1997 a。稻米儲藏性蛋白質性質與品質改進。科學農業。45:200-206。 簡珮如、盧虎生、朱鈞。1997 b。栽培時期對稻米儲藏性蛋白質之影響。中華農藝。7:333-342。 魏國彥、許晃雄。1997。近千年來的氣候變遷與全球暖化。全球環境變遷導論。2:12-19。教育部。 Abe T, Gusti RS, Ono M, Sasahara T (1996) Variations in glutelin and high molecular weight endosperm proteins among subspecies of rice (Oryza sativa L.) detected by two-dimensional gel electrophoresis. Genes Genet Syst 71:63-68 Adachi T, Izumi H, Yamada T, Tanaka K, Takeuchi S, Nakamura R, Matsuda T (1993) Gene structure and expression of rice seed allergenic proteins belonging to the alpha-amylase/trypsin inhibitor family. Plant Mol Biol 21:239-48 Ann M, Annick De K, Marc Van M (1997) A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response. Gene 191:197-204 Bradford MM (1976)A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72:248-254 Bouchez D, Hofte H (1998) Functional genomics in plants. Plant Physiol 118:725-32 Cai K, Luo S (1999)Effect of shading on growth, development and yield formation of rice. Yingyong Shengtai Xuebao 10:193-196 Eggum BO, Satoh H, Juliano BO (1994) Protein quality evaluation of cooked rice for protein mutants in growing rats. Cereal Chem 71:199-201 Fida MA, Setsuko K (2004)A proteomic approach to analyze salt-responsive proteins in rice leaf sheath. Proteomics 4: 2072-2081 Harris ELV, Angal S (1989)Protein purification methods:a practical approach. pp15-19. IRL Press, Oxford University Press, Oxford, New York. Hayama R, Izawa T, Shimamoto K (2002) Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method. Plant Cell Physiol 43:494-504 Huebner FR, Hussain A, Lookhart GL, Bietz JA, Bushuk W, Juliano BO (1991)Discrimination of sister-line IR rice varieties by polyacrylamide gel electrophoresis and reversed-phase high-performance liquid chromatography. Cereal Chem 68:583-588 Ishihara K, Kuroda E (1986) Effects of the shading and rachis-branch clipping on the grain-filling process of rice Oryza sativa cultivars differing in the grain size. Jpn Jour Crop Sci 55:252-260 Islam MS, Morison JIL (1992) Influence of solar radiation and temperature on irrigated rice grain yield in Bangladesh. Field Crops Research 30:13-28 Izumi H, Adachi T, Fujii N, Matsuda T, Nakamura R, Tanaka K, Urisu A, Kurosawa Y (1992) Nucleotide sequence of a cDNA clone encoding a major allergenic protein in rice seeds. Homology of the deduced amino acid sequence with members of alpha-amylase / trypsin inhibitor family. FEBS Lett 302:213-216 Juliano BO(1971)A simplified assay for milled rice amylase. Cereal Chem 16:334-340 Karine G, Christine LS, Joel V, Richard DT, Judith B (2003) Proteomics of Medicago truncatula seed development establishes the time frame of diverse metabolic processes related to reserve accumulation. Plant Physiol 133:664-682 Kim WT, Okita TW (1988)Structure, expression, and heterogeneity of the rice seed prolamines. Plant Physiol 88:649-655 Kobata T, Sugawara M, Takatu S (2000)Shading during the early filling period does not affect potential grain dry matter increase in rice. Agron J 92:411-417 Komatsu S, Konishi H, Shen S, Yang G (2003)Rice proteomics:A step toward functional analysis of the rice genome. Mol Cell Proteomics 2:2-10 Koller A, Washburn MP, Lange BM, Andon NL, Deciu C, Haynes PA, Hays L, Schieltz D, Ulaszek R, Wei J, Wolters D, Yates JR (2002)Proteomic survey of metabolic pathways in rice. Proc Natl Acad Sci 99(18):11969-11974 Komatsu S, Kojima K, Suzuki K, Ozaki K, Higo K (2004)Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis:its status in 2003. Nucleic Acids Res 32:388-392 Krishnan HB, Franceschi VR, Okita TW (1986)Immunochemical studies on the role of the golgi complex in protein-body formation in rice seeds. Planta 169:471-480 Kumamaru T, Satoh H, Iwata N, Omura T, Ogawa M, Tanaka K (1988)Mutants for semidwarfism-related proteins and glutelin seed protein in rice (Oryza sativa L.). Theor Appl Genet 83:153-158 Lasztity R (1986) The chemistry of cereal proteins. CRC Press, Boca Raton, FL. USA. Li X, Okita TW (1993)Accumulation of prolamines and glutelins during rice seed development:a quantitative evalutation. Plant Cell Physiol 34:385-390 Lin SK, Chang MC, Tsai YG, Lur HS (2005)Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics 5:2140-2156 Liu SC, Wang CH, Shin CJ, Chang HW, Hsiao CK, Liaw SH (2002)Reduction in sunshine duration over Taiwan: Causes and implications. TAO 13:523-545 Martin H, Ashwin G, Joel WS, Jay JT (2005) A systematic proteomic study of seed filling in soybean. Establishment of high-resolution two-dimensional reference maps, expression profiles, and an interactive proteome database. Plant Physiol 137:1397-1419 Martin H, Fitzgerald MA (2002)Proteins in rice grains influence cooking properties. Jour Cereal Sci 36:285-294 Masumura T, Mitsukawa N, Tanaka K, Fujii S (1991)Rice storage proteins:Genetic analysis of accumulation process. Biotechnology in Agriculture and Forestry. Berlin. New York. pp495-507, USA. Matsue Y, Furuno K, Yoshida T (1992)Effect of shading treatments during the mid-to late-ripening stages on palatability and physicochemical properties of rice. Jpn Jour Crop Sci 61:218-222 Matsuo T, Kumazawa K, Ishii R, Ishihara K, Hirata H (1995)Science of the Rice Plant. Vol II:598-695. Food and Agriculture Policy Research Center, Tokyo. Matsue Y, Odahara K, Hiramatsu M(1995)Differences in amylose content, amylographic characteristics and storage proteins of grains on primary and secondary rachis branches in rice. Jpn Jour Crop Sci 64:601-606 Michel Z, Dominique de V (2000) Proteomics:a link between genomics, genetics and physiology. Plant Mol Biol 44:575-580 O’Farrell PZ, Goodman HM, O’Farrell K (1977)High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133-1142 Ogawa M, Kumamaru T, Satoh H, Iwata N, Omura T, Kasai Z, Tanaka K (1987)Purification of protein body-I of rice seed and its polypeptide composition. Plant Cell Physiol 28:1517-1527 Ohtsubo K, Richardson M (1992)The amino acid sequence of a 20 kDa bifunctional subtilisin / alpha-amylase inhibitor from bran of rice (Oryza sativa L.)seeds. FEBS Lett 309:68-72 Okawa S, Makino A, Mae T (2003)Effect of irradiance on the partitioning of assimilated carbon during the early phase of grain filling in rice. Annals of Botany 92:357-364 Ou-Lee TM, Setter TL (1985)Enzyme activities of starch and sucrose pathways and growth of apical and basal maize kernels. Plant physiol 79:848-851 Rakwal R, Agrawal GK (2003)Rice proteomics:current status and future perspectives. Electrophoresis 24:3378-3389 Resurreccion AP, Li X, Okita TW, Juliano BO (1993)Characterization of poorly digested protein of cooked rice protein bodies. Cereal Chem 70:101-104 Sabaruddin Z, Toshiaki M, Shingo T, Youji N (2002)Effect of high temperature at ripening stage on the reserve accumulation in seed in some rice cultivars. Plant Prod Sci 5:160-168 Setter TL, Conocono EA, Egdane JA (1996)Possibility of increasing yield potential of rice by reducing panicle height in the Canopy II. Canopy photosynthesis and yield of isogenic lines. Austr Jour Plant Physiol 23:161-169 Seo SW, Chamura S (1980)Occurrence of varietal differences in protein, phosphorous, and potassium content in brown rice, and influence of temperature and shading during the ripening period on it. Jpn Jour Crop Sci 19:199-204 Sivalinganna M, Martin M S (1997)Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family and its expression during anoxia. Plant Mol Biol 33:97-112 Sugimoto T, Tanaka K, Kasai Z (1986)Improved extraction of rice prolamine. Agric Biol Chem 50:2409-2411 Surajit K DE DATTA(1981)Principles and Practices of Rice Production. pp12-15, IRRI, Philippines. Takashi O, Kanako H, Takashi S, Toshiaki I, Horst L, Tomokazu K, Erhard K (2004)Identification of major proteins in maize egg cells. Plant Cell Physiol 45:1406-1412 Tamaki M, Tashiro T, Yamamoto Y (1997)Effect of shading and fertilized nitrogen levels on the growth, yield components and physicochemical properties of rice. Jpn Jour Crop Sci 66:708-709 Tanaka K, Sugimoto T, Ogawa M, Kasai Z (1980)Isolation and characterization of two types of protein bodies in the rice endosperm. Agric Biol Chem 44:1633-1639 Toshinori ASG, Mayumi Ray O, Takeo S (1996)Variations in glutelin and high molecular weight endosperm proteins among subspecies of rice (Oryza sativa L.)detected by two-dimensional gel electrophoresis. Genes Genet Sys 71:63-68 Tsuno Y, Yamaguchi T(1989) Adaptive regulation of photosynthesis in rice plant to weak light condition and the contribution of root activity to regulation mechanism. Jpn Jour Crop Sci 58:74-83 Wen TN, Luthe DS (1985)Biochemical characterization of rice glutelin. Plant Physiol 78:172-177 Westermeier R (1993)Electrophoresis in practice:a guide to theory and practice. pp167-171. Weinheim, New York. Xu Y, Harris-Haller LW, McCollum JC, Hardin SH, Hall TC (1993)Nuclear gene encoding cytosolic triosephosphate isomerase from rice(Oryza sativa L.). Plant Physiol 102:697 Yamagata H, Tanaka K (1986)The site of synthesis and accumulation of rice storage proteins. Plant Cell Physiol 27:135-145 Yamagata H, Sugimoto T, Tanaka K, Kasai Z(1982)Biosynthesis of storage proteins in development rice seeds. Plant Physiol 70:1094-1100 Yan S, Tang Z, Su W, Sun W (2005)Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics 5:235-244 Yasuzo K, Yasuhiro I, Naoto O, Shin-ya K (1991)Isolation and determination of protein bodies(PB-I, PB-II)in polished rice endosperm. J Brew Soc Japan 86(4):293-298 Yoshida S, Parao FT (1976)Climatic influences on yield and yield components of lowland rice in the tropics, In “Climate and rice”.pp471-494, IRRI, Los Banos Yukihiko I, Kunio Y, Toshiaki K, Ritsuo S (2002)Comparison of the occurrence of kernel damage in rice plant growth in a heated greenhouse and in a paddy filed of high temperature year. Jpn Jour Crop Sci 71:174-177 Yumiko U, Masayuki N, Hirotaka H, Atsuo U, Naohito A, Ken K, Tsukasa M (2001) A 33-kDa allergen from rice (Oryza sativa L. Japonica). cDNA Cloning, expression, and identification as a novel glyoxalase I. Jour Biol Chem 276:11376–11381 Zhao WM, Gatehouse JA, Boulter D (1983)The purification and partial amino acid sequence of a polypeptide from the glutelin fraction of rice grains:homology to pea legumin. FEBS Lett 162:96-102 Zhongyi L, Sun F, Shoumin X, Xiusheng C, Mukai Y, Yamamoto M, Shahjahan A, Rampling L, Kosar-Hashemi B, Rahman S, Morell MK (2003) The structural organization of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants. Funct Integr Genomics 3:76-85 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36572 | - |
dc.description.abstract | 光是光合作用的能量來源,也是調控作物從種子萌發到開花結實的生長發育過程。在實際栽培上作物品種的株型及田間的種植密度皆可影響植冠(canopy)的光照環境。良質米的栽培需要適宜的環境條件,台灣一、二期稻作栽培環境的光強度變化很大,被認為是影響一、二期稻米品質的主因之一。蛋白質對米粒的理化性質、營養價值及外觀性狀有決定性的影響,因此研究蛋白質生合成的過程有助於稻米品質的改進。
本試驗主要的目的為探討光強度對水稻榖粒蛋白質表現與貯藏性蛋白質生合成與累積的影響及其與品質形成的相關性。以蛋白質分析技術上具有高解析度的蛋白質雙向電泳分析,配合proteomics之觀念,將在遮蔭處理下具有差異的蛋白質分子進行胺基酸序列鑑定。本試驗使用三個良質米品種(台中秈10號號、台稉9號、越光),日夜溫25/20 ℃,對照組與處理組(遮蔭50%),三個生育時期,共18個處理組合。試驗分析包括光強度紀錄、穗重、葉綠素含量、光合作用速率、米粉性質(RVA)、蛋白質含量、積溫、各貯藏性蛋白質的變化及稻米理化性質。再以蛋白質雙向電泳圖譜在各處理條件下的變化,藉以建立生理與蛋白質雙向電泳圖譜間的關聯。 試驗結果顯示,在生理分析上,越光、台中秈10號、台稉9號在遮蔭處理下,穗重、粒重的累積速率減緩;葉綠素含量(SPAD值)則降低2~3、光合作用速率下降約50%;積溫需求以越光增加最明顯,其次為台中秈10號,最低為台稉9號,分別增加213、202、157 degree-day;抽穗到成熟累積天數延長約7~8天,反應低光照會延遲水稻穀粒的充實生理過程。米粉性質之Peak Viscosity、Final Viscosity、Setback、Breakdown值因遮蔭處理而下降;直鏈性澱粉含量在遮蔭處理下含量皆下降;而鹼性擴散程度在遮蔭處理下,越光屬於中間糊化溫度,台稉9號屬於中高糊化溫度,台中秈10號則仍和在對照組時相同,為中高糊化溫度;膠體展延性方面,沒有明顯的差異;成熟種子總蛋白質含量則因遮蔭增加約50%。 電泳圖譜分析顯示,單向 SDS-PAGE分析方面,越光、台中秈10號、台稉9號在遮蔭處理下,其穀粒發育期之prolamins、glutelins之表現皆增加;而雙向 SDS-PAGE方面,越光、台中秈10號、台稉9號其穀粒發育期間大約可以觀察到400~500個蛋白質點,台中秈10號其蛋白質點在遮蔭處理下受到較大的影響,而且蛋白質總量上較越光、台稉9號大,可能是低光照影響了貯藏性蛋白質累積的種類及生合成途徑;此外,三品種經遮蔭處理,各prolamins、glutelins之各單元分子(subunit)表現也增加,但增加不同步。台中秈10號之prolamins、glutelins累積量最明顯,台稉9號之glutelins累積量增加,但prolamins則無明顯變化,此結果與總蛋白質含量測定的結果符合。進一步以RT-PCR方法分析在遮蔭處理下相關基因之RNA表現情形,Prolamin、Glutelin、SSII均有明顯量上的差異,GBSS、NDK則在對照組與處理組之間沒有量上的差異;此結果與蛋白質電泳膠片所顯示的結果相符。將差異表現之30個蛋白質點收集後,以MALDI-TOF MS/MS或ESI-Q-TOF進行蛋白質身份鑑定,再將其分析後所得之資料以Mascot Search進行MS/MS Ions Search比對,得到定序並確定的蛋白點包括:5個prolamins、4個glutelins、3個glutelin precursor、2個glyceraldehyde 3-phosphate dehydrogenase(G3PDH)、fructose-bisphosphate aldolase isoenzyme C-1(FBPA)、glyoxalase I、cytoplasmic malate dehydrogenase、glycogen starch synthase、peroxiredoxin、putative stress-related protein、alanine aminotransferase、bifunctional subtilisin/alpha-amylase inhibitor(BSAI)、group 3 LEA (type I) protein、allergen RA5B precursor、triose-phosphate isomerase(TPI)、major allergenic protein (MAP)、UDP-glucose pyrophosphorylase,這些蛋白質大多因遮蔭而表現量增加,少數隨遮蔭而表現量減少或消失,這可能跟該蛋白質在穀粒發育期間所扮演的角色相關,本研究將各蛋白質的表現與穀粒發育期的過程進行分析歸納,推論這些蛋白質與光照及稻米品質形成的關係。 | zh_TW |
dc.description.abstract | Light is one of crucial environmental factors affecting rice yield and quality. The purpose of the present approach was to study the effects of light on the quality formation of rice grains, utilizing proteomic related techniques.
Three quality rice cultivars were grown in a phytotron and shading(50%)treatment were performed at flowering stage. Rice grains were sampled at 6 days, 15 days and mature after flowering(DAF)for analysis. Physiological characteristics, yield traits, and physical-chemical properties of rice grains were determined. For molecular analysis, changes of proteome expression pattern were monitored by one or two dimension electrophoresis(SDS-PAGE or 2-DE). Differential expressed proteins were selected for putative function identification using MALDI-TOF and ESI-Q-TOF. The relationships between functions of proteins and the effects of light intensity on rice grain quality were analyzed and discussed. The results showed that, plant growth and all physiological processes were slowed down under shade. Cultivar TCS10 was more sensitive to the light intensity reduction than Koshihikari and TK9. Under shading treatment, three cultivars panicle and grain weight accumulation rate was slow down. Chlorophyll content (SPAD value) was decreased, and photosynthesis activity was dropped by about 50%. Under shading the time to mature was prolonged for about one week, suggesting more heat units is necessary to complement the low light effect. Low light intensity increased grain protein content and decreased amylose content. In physical-chemical related properties revealed by RVA, peak viscosity, breakdown, and final viscosity were decreased by low light treatment. In proteomic analysis a total of 400 to 500 protein spots could be resolved by 2-DE gels. Expression patterns of protein spots for three cultivars were significantly affected by shading. More than 30 differential expressed proteins were selected, identified with their pI and mass, and quantified. During rice grain development the amount of 7 proteins in Koshihikari, 18 proteins in TCS10 and 10 proteins in TK9 were increased. And the levels of 20 proteins in Koshihikari, 5 proteins in TCS10 and 14 proteins in TK9 were decreased during rice grain development. Shading increased the expression of prolamins, glutelins, G3PDH, FBPA, and alanine aminotransferase, but decreased the expression of glyoxalase I, cytoplasmic malate dehydrogenase, glycogen starch synthase, and several allergen proteins. The decrease of glycogen starch synthase might be associated with the decrease of amylose content of rice grains grown under low light. And the increase of the amount of glutelins and prolamins may influence the physical-chemical characteristics of rice flour. In conclusion, the present study provided a proteomic and functional basis that can be used as a tool to monitor and rationalize the effect of light on the rice grain quality formation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T08:06:05Z (GMT). No. of bitstreams: 1 ntu-94-R92621101-1.pdf: 5588635 bytes, checksum: 82718338491e09a9f523b9fe31f8c784 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄
表目錄………………………………………………………………I 圖目錄………………………………………………………………Ⅱ 縮寫字對照表………………………………………………………Ⅳ 中文摘要……………………………………………………………Ⅴ 英文摘要……………………………………………………………Ⅷ 前言…………………………………………………………………1 前人研究……………………………………………………………2 試驗架構……………………………………………………………10 材料與方法…………………………………………………………11 結果…………………………………………………………………24 討論…………………………………………………………………61 結論…………………………………………………………………73 參考文獻……………………………………………………………75 | |
dc.language.iso | zh-TW | |
dc.title | 光強度對水稻榖粒蛋白質表現及品質形成之影響 | zh_TW |
dc.title | Effects of light intensity on the expression of grain proteins and quality formation in rice (Oryza sativa L.) | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 朱鈞,陳宗禮,洪梅珠 | |
dc.subject.keyword | 水稻,光強度,蛋白質,米質, | zh_TW |
dc.subject.keyword | rice,light intensity,proteins,quality, | en |
dc.relation.page | 82 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-21 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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