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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 姚宗珍 | |
dc.contributor.author | Chu-Yin Weng | en |
dc.contributor.author | 翁竹音 | zh_TW |
dc.date.accessioned | 2021-06-13T08:02:16Z | - |
dc.date.available | 2008-08-02 | |
dc.date.copyright | 2005-08-02 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-22 | |
dc.identifier.citation | 1. Abiko Y, Shimizu N, Yamaguchi M, Suzuki H, Takiguchi H. Effect of aging on functional changes of periodontal tissue cells. Ann. Periodontol. 3(1): 350-369, 1998.
2. Ajubi NE, Klein-Nulend J, Nijweide PJ, Vrijheid-Lammers T, Alblas MJ, Burger EH. Pulsating fluid flow increases prostaglandin production by cultured chicken osteocytes--a cytoskeleton-dependent process. Biochem. Biophys. Res. Commun. 225(1): 62-68, 1996. 3. Akhouayri O, Lafage-Proust MH, Rattner A, Laroche N, Caillot-Augusseau A, Alexandre C, Vico L. Effects of static or dynamic mechanical stresses on osteoblast phenotype expression in three-dimensional contractile collagen gels. J. Cell. Biochem. 76(2): 217-230, 1999. 4. Basso N, Heersche JN. Characteristics of in vitro osteoblastic cell loading models. Bone 30(2): 347-351, 2002. 5. Bishop GA, Rokahr KL, Lowes M, McGuinness PH, Napoli J, Decruz DJ. Quantitative reverse transcriptase-PCR amplification of cytokine mRNA in liver biopsy specimens using a non-competitive method. Immunol. Cell Biol. 75: 142-147, 1997. 6. Bord S, Horner A, Beeton CA, Hembrt RM, Compston JE. Tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) distribution in normal and pathological human bone. Bone 24(3): 229-235, 1999. 7. Bord S, Horner A, Hembry RM, Compston JE. Stromelysin-1 (MMP-3) and Stromelysin-2 (MMP-10) expression in developing human bone: potential role in skeletal development. Bone 23(1): 7-12, 1998. 8. Brighton CT, Fisher JR, Levine SE, Corsetti JR, Reilly T, Landsman AS, Williams JL, Thibault LE. The biochemical pathway mediating the proliferative response of bone cells to a mechanical stimulus. J. Bone Jt. Surg. (Am.) 78(9): 1337-1347, 1996. 9. Brighton CT, Sennett BJ, Farmer JC, Iannotti JP, Hansen CA, Williams JL, Williamson J. The inositol phosphate pathway as a mediator in the proliferative response of rat calvarial bone cells to cyclic biaxial mechanical strain. J. Orthop. Res. 10: 385-393, 1992. 10. Brighton CT, Strafford B, Gross SB, Leatherwood DF, Williams JL, Pollack SR. The proliferative and synthetic response of isolated calvarial bone cells of rats to cyclic biaxial mechanical strain. J. Bone Jt. Surg. (Am.) 73(3): 320-331, 1991. 11. Brown TD. Techniques for mechanical stimulation of cells in vitro: a review. J. Biomech. 33(1): 3-14, 2000. 12. Burger EH, Klein-Nulend J, Veldhuijzen JP. Mechanical stress and osteogenesis in vitro. J. Bone Miner. Res. 7(Suppl 2): S397-S401, 1992. 13. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain. J. Mol. Endocrinol. 25: 169-193, 2000. 14. Cho NH, Hong KP, Hong SH, Kang S, Chung KY, Cho SH. MMP expression profiling in recurred stage IB lung cancer. Oncogene 23(3): 845-851, 2004. 15. Delaisse JM, Andersen TL, Engsig MT, Henriksen K, Troen T, Blavier L. Matrix metalloproteinases (MMP) and cathepsin K contribute differently to osteoclastic activities. Microsc. Res. Tech. 61(6): 504-513, 2003. 16. Delaisse JM, Engsig MT, Everts V, del Carmen Ovejero M, Ferreras M, Lund L, Vu TH, Werb Z, Winding B, Lochter A, Karsdal MA, Troen T, Kirkegaard T, Lenhard T, Heegaard AM, Neff L, Baron R, Foged NT. Proteinases in bone resorption: obvious and less obvious roles. Clinica. Chimica. Acta. 291(2): 223-234, 2000. 17. Denhardt DT, Burger EH, Kazanecki C, Krishna S, Semeins CM, Klein-Nulend J. Osteopontin-deficient bone cells are defective in their ability to produce NO in response to pulsatile fluid flow. Biochem. Biophys. Res. Commun. 288(2): 448-453, 2001. 18. Dinarello CA. Biologic basis for interleukin-1 in disease. Blood 87(6): 2095-2147, 1996. 19. Elliot KJ, Millward SJ, Wright MO, Robb JE, Wallace WHB, Salter DM. Effect of methotrexate on human bone cell responses to mechanical stimulation. Rheumatology 43: 1226-1231, 2004. 20. Engsig MT, Chen QJ, Vu TH, Peddersen AC, Therkidsen B, Lund LR, Henriksen K, Lenhard T, Foged NT, Werb Z, Delaisse JM. Matrix metalloproteinase 9 and vascular endotheial growth factor are essential for osteoclast recruitment into developing long bones. J. Cell Biol. 151: 879-889, 2000. 21. Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Banes AJ, Guilak F. The effect of static and intermittent compression on nitric oxide production in articular cartilage explants. J. Orthop. Res. 000: 000-000, 2001. 22. Foos MJ, Hickox JR, Mansour PG, Slauterbeck JR, Hardy DM. Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament. J. Orthop. Res. 19: 642-649, 2001. 23. Forwood MR. Inducible cyclo-oxygenase (COX-2) mediates the induction of bone formation by mechanical loading in vivo. J. Bone Miner. Res. 11(11): 1688-1693, 1996. 24. Giambernardi TA, Grant GM, Taylor GP, Hay RJ, Maher VM, McCormick JJ, Klebe RJ. Overview of matrix metalloproteinase expression in cultured human cells. Matrix Biol. 16(8): 483-496, 1998. 25. Glanstchnig H, Varga F, Rumpler M, Klaushofer K. Prostacyclin (PGI2): a potential mediator of c-fos expression induced by hydrostatic pressure in osteoblastic cells. Eur. J. Clin. Invest. 26: 544-548, 1996. 26. Hirumay Y, Inoue A, Shiohama A, Otsuka E, Hirose S, Yamaguchi A, Hagiwar H. Endothelins inhibit the mineralization of osteoblastic MC3T3-E1 cells through the A-type endothelin receptor. Am. J. Physiol. Regul. Integr. Comp. Physiol. 275: 1099-1105, 1998. 27. Holliday LS, Welgus HG, Fliszar CJ, Veith GM, Jeffrey JJ, Gluck SL. Initiation of osteoclast bone resorption by interstitial collagenase. J. Biol. Chem. 272: 22053-22058, 1997. 28. Holmbeck K, Bianco P, Caterina J, Yamada S, Kromer M, Kuznetsov SA, Mankani M, Robey PG, Poole AR, Pidoux I, Ward JM, Birkedal-Hansen H. MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue due to inadequate collagen turnover. Cell 99: 81-92, 1999. 29. Hughes-Fulford M. Signal transduction and mechanical stress. Sci. STKE 249:re12, 2004. 30. Ignatius A, Blessing H, Liedert A, Schmidt C, Neidlinger-Wilke C, Kaspar D, Friemert B, Claes L. Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices. Biomaterials 26: 311-318, 2005. 31. Imamura K, Ozawa H, Hiraide T, Takahashi N, Shibasaki Y, Fukuhara T, Suda T. Continuously applied compressive pressure induce bone resorption by a mechanism involving prostaglandin E2 synthesis. J. Cell. Physiol. 144: 222-228, 1990. 32. Jensen LE, Muzio M, Mantovani A, Whitehead AS. IL-1 signaling cascade in liver cells and the involvement of a soluble form of the IL-1 receptor accessory. J. Immunol. 164: 5277-5286, 2000. 33. Jensen LE, Whitehead AS. The 3' untranslated region of the membrane-bound IL-1R accessory protein mRNA confers tissue-specific destabilization. J. Immunol. 173(10): 6248-6258, 2004. 34. Judex S, Zhong N, Squire ME, Ye K, Donahue LR, Hadjiargyrou M, Rubin CT. Mechanical modulation of molecular signals which regulate anabolic and catabolic activity in bone tissue. J. Cell. Biochem. 94(5): 982-994, 2005. 35. Kanzaki H, Chiba M, Shimizu Y, Mitani H. Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. J. Bone Miner. Res. 17(2): 210-220, 2002. 36. Karne S, Jayawickreme CK, Lerner MR. Cloning and characterization of an endothelin-3 specific receptor (ETC receptor) from Xenopus laevis dermal melanophores. J. Biol. Chem. 268(25): 19126-19133, 1993. 37. Kaspar D, Seidl W, Neidlinger-Wilke C, Beck A, Claes L. Ignatius a proliferation of human-derved osteoblast-like cells depends on the cycle number and frequency of uniaxial strain. J. Biomech. 35(7): 873-880, 2002. 38. Kaspar D, Seidl W, Neidlinger-Wilke C, Ignatius A, Claes L. Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity. J. Biomech. 33(1): 45-51, 2000. 39. Kawata A, Mikuni-Takagaki Y. Mechanotransduction in stretched osteocytes --temporal expression of immediate early and other genes. Biochem. Biophys. Res. Commun. 246(2): 404-408, 1998. 40. Kerkela E, Ala-aho R, Lohi J, Grenman R, M-Kahari V, Saarialho-Kere U. Differential patterns of stromelysin-2 (MMP-10) and MT1-MMP (MMP-14) expression in epithelial skin cancers. Br. J. Cancer 84(5): 659-669, 2001. 41. Kinoshita S, Finnegan M, Bucholz RW, Mizuno K. Three-dimensional collagen gel culture promotes osteoblastic phenotype in bone marrow derived cells. Kobe J. Med. Sci. 45(5): 201-211, 1999. 42. Klein-Nulend J, Roelofsen J, Semeins CM, Bronckers AL, Burger EH. Mechanical stimulation of osteopontin mRNA expression and synthesis in bone cell cultures. J. Cell. Physiol. 170(2): 174-181, 1997. 43. Klein-Nulend J, Roelofsen J, Sterck JGH, Semeins CM, Burger EH. Mechanical loading stimulates the release of transforming growth factor-β activity by cultured mouse calvariae and periosteal cells. J. Cell. Physiol. 163: 115-119, 1995. 44. Klein-Nulend J, Veldhuijzen JP, Burger EH. Increased calcification of growth plate cartilage as a result of compressive force in vitro. Arthritis Rheum. 29(8): 1002-1009, 1986. 45. Kniss DA. Cyclooxygenases in reproductive medicine and biology. Journal of the Society for Gynecologic Investigation. J. Soc. Gynecol. Investig. 6(6): 285-292, 1999. 46. Konturek PC, Rembiasz K, Konturek SJ, Stachura J, Bielanski W, Galuschka K, Karcz D, Hahn EG. Gene expression of ornithine decarboxylase, cyclooxygenase-2, and gastrin in atrophic gastric mucosa infacted with Helicobacter pylori before and after eradication therapy. Dig. Dis. Sci. 48(1): 36-46, 2003. 47. Kubota T, Yamauchi M, Onozaki J, Sato S, Susuki Y, Sodek J. Influence of an intermittent compressive force on matrix protein expression by ROS 17/2.8 cells, with selective stimulation of osteopontin. Archs. Aral. Bio. 38(1): 23-30, 1993. 48. Lambert GL, Barker S, Lees DM, Corder R. Endothelin-2 synthesis is stimulated by the type-1 tumour necrosis factor receptor and cAMP: comparison with endothelin-converting enzyme-1 expression. J. Mol. Endocrinol. 24: 273-283, 2000. 49. Lapp CA, Lohse JE, Lewis JB, Dickinson DP, Billman M, Hanes PJ, Lapp DF. The effects of progesterone on matrix metalloproteinases in cultured human gingival fibroblasts. J. Periodontol. 74(3): 277-288, 2003. 50. Lin PM, Chen CT, Torzilli PA. Increased stromelysin-1 (MMP-3), proteoglycan degradation (3B3- and 7D4) and collagen damage in cyclically load-injured articular cartilage. Osteoarthritis cartilage 12(6): 485-496, 2004. 51. Long P, Liu F, Piesco NP, Kapur R, Agarwal S. Signaling by mechanical strain involves transcriptional regulation of proinflammatory genesin human periodontal ligament cells in vitro. Bone 30(4): 547-552, 2002. 52. Lozupone E, Palumbo C, Favia A, Ferrrtti M, Cantatore FP. Intermittent compressive load stimulates osteogenesis and improves osteocyte viability in bones cultured “in vitro”. Clin. Rheumatol. 15(6): 563-572, 1996. 53. Martignetti JA, Aqeel AA, Sewairi WA, Boumah CE, Kambouris M, Mayouf SA, Sheth KV, Eid WA, Dowling O, Harris J, Glucksman MJ, Bahabri S, Meyer BF, Desnick RJ. Mutation of the matrix metalloproteinase 2 gene (MMP-2) causes a multicentric osteolysis and arthritis syndrome. Nat. Genet. 28(3): 261-265, 2001. 54. Masi L, Franchi A, Santucci M, Danielli D, Arganini L, Giannone V. Adhesion, growth, and matrix production by osteoblasts on collagen substrata. Calcif. Tissue Int. 51(3): 202-212, 1992. 55. McAllister TN, Frangos JA. Steady and transient fluid shear stress stimulate NO release in osteoblasts through distinct biochemical pathways. J. Bone Miner. Res. 14(6): 930-936, 1999. 56. Mohammad KS, Guise TA. Mechanisms of osteoblastic metastases: role of endothelin-1. Clin. Orthop. Relat. Res. 415(Suppl): S67-S74, 2003. 57. Muller D, Breathnach R, Engelmann A, Millon R, Bronner G, Flesch H, Dumont P, Eber M, Abecassis J. Expression of collagenase-related metalloproteinase genes in human lung or head and neck tumours. Int. J. Cancer 48(4): 550-556, 1991. 58. Nagatomi J, Arulanandam BP, Metzger DW, Meunier A, Bizios R. Cyclic pressure affects osteoblast functions pertinent to osteogenesis. Ann. Biomed. Eng. 31(8): 917-923, 2003. 59. Nakamura H, Fujii Y, Ohuchi E, Yamamoto E, Okada Y. Activation of the precursor of human stromelysin 2 and its interactions with other matrix metalloproteinases. Eur. J. Biochem. 253(1): 67-75, 1998. 60. Ohba Y, Ohba T, Terai K, Moriyama K. Expression of cathepsin K mRNA during experimental tooth movement in rat as revealed by in situ hybridization. Arch. Oral Biol. 45(1): 63-69, 2000. 61. Ohzeki K, Yamaguchi M, Shimizu N, Abiko Y. Effect of cellular aging on the induction of cyclooxygenase-2 by mechanical stress in human periodontal ligament cells. Mech. Ageing Dev. 108(2): 151-163, 1999. 62. Origuchi T, Migita K, Nakashima T, Honda S, Yamasaki S, Hida A, Kawakami A, Aoyagi T, Kawabe Y, Eguchi K. Regulation of cyclooxygenase-2 expression in human osteoblastic cells by N-acetylcysteine. J. Lab. Clin. Med. 136(5): 390-394, 2000. 63. Ozawa H, Imamura K, Abe E, Takahashi N, Hiraide T, Shibasaki Y, Fukuhara T, Suda T. Effect of a continuously applied compressive pressure on mouse osteoblast-like cells (MC3T3-E1) in vitro. J. Cell. Physiol. 142: 177-185, 1990. 64. Patwari P, Fay J, Cook MN, Badger AM, Kerin AJ, Lark MW. In vitro models for investigation of the effects of acute mechanical injury on cartilage. Clin. Orthop. 391: S61-S71, 2001. 65. Pavalko FM, Chen NX, Turner CH, Burr DB, Atkinson S, Hsieh YF, Qiu J, Duncan RL. Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions. Am. J. Physiol. 275(6 Pt 1): C1591-C1601, 1998. 66. Pioletti DP, Müller J, Rakotomanana LR, Corbeil J, Wild E. Effect of micromechanical stimulation on osteoblasts development of a device simulating the mechanical situation at the bone-implant interface. J. Biomech. 36: 131-135, 2003. 67. Rechardt O, Elomaa O, Vaalamo M, Paakkonen K, Jahkola T, Hook-Nikanne J, Hembry RM, Hakkinen L, Kere J, Saarialho-Kere U. Stromelysin-2 is upregulated during normal wound repair and is induced by cytokines. J. Invest. Dermatol. 115(5): 778-787, 2000. 68. Rifas L, Fausto A, Scott MJ, Avioli LV, Welgus HG. Expression of metalloproteinases and tissue inhibitors of metalloproteinases in human osteoblast-like cells: Differentiation is associated with repression of metalloproteinase biosynthesis. Endocrinology 134: 213-221, 1994. 69. Roelofsen J, Klein-Nulend J, Burger EH. Mechanical stimulation by intermittent hydrostatic compression promotes bone-specific gene expression in vitro. J. Biomech. 28(12): 1493-1503, 1995. 70. Rubany GM, Polokoff MA. Endothelins: Molecular biology, biochemistry, pharmacology, physiology, and pathophysiology. Pharmacol. Rev. 46: 325-415, 1994. 71. Saghizadeh M, Brown DJ, Castellon R, Chwa M, Huang GH, Ljubimova JY, Rosenberg S, Spirin KS, Stolitenko RB, Adachi W, Kinoshita S, Murphy G, Windsor LJ, Kenney MC, Ljubimov AV. Overexpression of matrix metalloproteinase-10 and matrix metalloproteinase-3 in human diabetic corneas: a possible mechanism of basement membrane and integrin alterations. Am. J. Pathol. 158(2): 723-734, 2001. 72. Sakai K, Mohtai M, Iwamoto Y. Fluid shear stress increases transforming growth factor beta 1 expression in human osteoblast-like cells: Modulation by cation channel blockades. Calcif. Tissue Int. 63: 515-520, 1998. 73. Sakoda S, Shin H, Yamaji K, Takasaki I, Furuzono T, Kishida A. Mechanical stretching of human osteoblast-like cells stimulates bone morphogenic proteins and macrophage colony-stimulating factor productions. Pathophysiology 6(1): 63-69, 1999. 74. Shimizu N, Ozawa Y, Yamaguchi M, Goseki T, Ohzeki K, Abiko Y. Induction of COX-2 expression by mechanical tension force in human periodontal ligament cells. J. Periodontol. 69(6): 670-677, 1998. 75. Shioide M, Noda M. Endothelin modulates osteopontin and osteocalcin messenger ribonucleic acid expression in rat osteoblastic osteosarcoma cells. J. Cell. Biochem. 53(2): 176-180, 1993. 76. Smalt R, Mitchell F, Howard R, Chambers T. Induction of NO and prostaglandin E2 in osteoblasts by wall-shear stress but not mechanical strain. Am. J. Physiol. 273: E751-E758, 1997. 77. Smeets RL, Van de Loo FA, Joosten LA, Arntz OJ, Bennink MB, Loesberg WA, Dmitriev IP, Curiel DT, Martin MU, Van den Berg WB. Effectiveness of the soluble form of the interleukin-1 receptor accessory protein as an inhibitor of interleukin-1 in collagen-induced arthritis. Arthritis Rheum. 48(10): 2949-2958, 2003. 78. Stanford CM, Morcuende JA, Brand RA. Proliferative and phenotypic responses of bone-like cells to mechanical deformation. J. Orthop. Res. 13(5): 664-670, 1995. 79. Sternlicht MD, Werb Z. How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell Dev. Biol. 17: 463-516, 2001. 80. Suzuki A, Shinoda J, Watanabe-Tomita Y, Ozaki N, Oiso Y, Kozawa O. ETA receptor mediates the signaling of endothelin-1 in osteoblast-like cells. Bone 21: 143-146, 1997. 81. Takai E, Mauck RL, Hung CT, Guo XE. Osteocyte viability and regulation of osteoblast function in a 3D trabecular bone explant under dynamic hydrostatic pressure. J. Bone Miner. Res. 19: 1403-1410, 2004. 82. Takuwa Y, Masaki T, Yamashita K. The effects of the endothelin family peptides on cultured osteoblastic cells from rat calvariae. Biochem. Biophys. Res. Commun. 170(3): 998-1005, 1990. 83. Tanabe N, Maeno M, Suzuki N, Fujisaki K, Tanaka H, Ogiso B, Ito K. IL-1alpha stimulates the formation of osteoclast-like cells by increasing M-CSF and PGE(2) production and decreasing OPG production by osteoblasts. Life Sci. 77(6): 615-626, 2005. 84. Tanaka SM, Li J, Duncan RL, Yokato H, Burr DB, Turner CH. Effects of broad frequency vibration on cultured osteoblasts. J. Biomech. 36(1): 73-80, 2003. 85. Tasevski V, Sorbetti JM, Chiu SS, Shrive NG, Hart DA. Influence of mechanical and biological signals on gene expression in human MG-63 cells: evidence for a complex interplay between hydrostatic compression and vitamin D3 or TGF-β1 on MMP-1 and MMP-3 mRNA levels. Biochem. Cell Biol. 83(1): 96-107, 2005. 86. Terai K, Takano-Yamamoto T, Ohba Y, Hiura K, Sugimoto M, Sato M, Kawahata H, Inaguma N, Kitamura Y, Nomura S. Role of osteopontin in bone remodeling caused by mechanical stress. J. Bone Miner. Res. 14(6): 839-849, 1999. 87. Tjandrawinata R, Vincent V, Hughes-Fulford M. Vibrational force alters mRNA expression in osteoblasts. FASEB J. 11: 493-497, 1997. 88. Tsuji K, Uno K, Zhang GX, Tamura M. Periodontal ligament cells under intermittent tensile stress regulate mRNA expression of osteoprotegerin and tissue inhibitor of matrix metalloprotease-1 and -2. J. Bone Miner. Metab. 22(2): 94-103, 2004. 89. Uchida M, Shima M, Shimoaka T, Fujieda A, Obara K, Suzuki H, Nagai Y, Ikeda T, Yamato H, Kawaguchi H. Regulation of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) by bone resorptive factors in osteoblastic cells. J. Cell. Physiol. 185(2): 207-214, 2000. 90. Wang L, Zhu H, Liang T. The changes of transforming growth factor beta 1 in periodontal tissue during orthodontic tooth movement. Chung-Hua Kou Chiang Hsueh Tsa Chih Chinese Journal of Stomatology 34(4): 242-244, 1999. 91. Westbroek I, Ajubi NE, Albas MJ, Semeins CM, Klein-Nulend J, Burger EH, Nijweide PJ. Differential stim ulation of prostaglandin G/H synthase-2 in osteocytes and other osteogenic cells by pulsating fluid flow. Biochem. Biophys. Res. Commun. 268: 414-419, 2000. 92. Willard MF, Daniel JR, Kent EV. Fundamentals of DNA hybridization arrays for gene expression analysis. Bio. Techniques 29: 1042-1055, 2000. 93. Woessner JF. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J. 5: 2145-2154, 1991. 94. Wong M, Siegrist M, Goodwin K. Cyclic tensile strain and cyclic hydrostatic pressure differentially regulate expression of hypertrophic markers in primary chondrocytes. Bone 33(4): 685-693, 2003. 95. Yamashiro T, Fukunaga T, Kobashi N, Kamioka H, Nakanishi T, Takigawa M, Takana-Yamamoto T. Mechanical stimulation induces CTGF expression in rat osteocytes. J. Dent. Res. 80(2): 461-465, 2001c. 96. Yanagisawa M, Inoue A, Takuwa Y, Mitsui Y, Kobayashi M, Masaki T. The human preproendothelin-1 gene: possible regulation by endothelial phosphoinositide turnover signaling. J. Cardiovasc. Pharmacol. 13: S13-S17, 1989b. 97. Yanagisawa M, Kimura S, Kasuya Y, Sawamura Y, Shinmi O, Sugita Y, Goto K, Madaki T. Concersion of big endothelin-1 to 21-residue endothelin-1 is essential for expression of full vasoconstrictor activity: structure-activity relationships of big endothelin-1. J. Cardiovasc. Pharmacol. 13: S5-S7, 1989a. 98. Yang CM, Chien CS, Yao CC, Hsiao LD, Huang YC, Wu CB. Mechanical strain induces collagenase-3 (MMP-13) expression in MC3T3-E1 osteoblastic cells. J. Biol. Chem. 279(21): 22158-22165, 2004. 99. Yoshihara Y, Nakamura H, Obata K, Yamada H, Hayakawa T, Fujikawa K, Okada Y. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in synovial fluids from patients with rheumatoid arthritis or osteoarthritis. Ann. Rheum. Dis. 59(6): 455-461, 2000. 100. Zerath E, Holy X, Roberts SG, Andre C, Renault S, Hott M, Marie PJ. Spaceflight inhibits bone formation independent of corticosteroid status in growing rats. J. Bone Miner. Res. 15: 1310-1320, 2000. 101. 許文婷:利用基因微陣列方法探討牙周韌帶細胞接受機械力量刺激後其基因表現之改變。國立陽明大學臨床牙醫學研究所碩士論文,2004。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36476 | - |
dc.description.abstract | 機械力量刺激對生長發育過程中骨骼重塑扮演重要的角色,但對力量刺激對細胞內所造成的基因變化影響,目前仍瞭解不多。為了篩選更多受壓力刺激影響之細胞基因,本研究利用類成骨母細胞株 (MG63 cell) 作為實驗對象,培養於3D膠原蛋白凝集體中,施予週期性壓力24小時。在帶有7680個基因的微陣列進行生物微陣列分析,經標準化後,共有43個基因在細胞受壓後有差異性表現,包括許多細胞凋亡、細胞週期調控、代謝、細胞增生、發炎反應等功能相關的基因,以及一些功能未知的基因。在進一步以半定量反轉錄-聚合酶鏈鎖反應 (RT-PCR) 分析,COX-2、ODC、MMP-3、MMP-10的表現上升,而ET-2、mIL-1RAcP的表現下降,符合微陣列之結果。以即時定量聚合酶連鎖反應 (Real-time PCR),發現COX-2 mRNA表現因壓力刺激提升2.27倍,而MMP-3 mRNA表現提升2.3倍。以西方點墨法分析細胞內MMP-3蛋白質的表現量,在壓力刺激下有微量上升的情形,但MMP-10蛋白質的表現量受機械壓力刺激後並無差異。以酵素活性分析法分析,MMP-3與MMP-10分解gelatin的活性微量受機械壓力刺激誘導增加表現。我們的結果支持著週期性壓力具有促進MG63細胞COX-2和MMP-3 mRNA表現,以及受壓後MMP-3蛋白質分泌也有增加。 | zh_TW |
dc.description.abstract | It is widely accepted that mechanical loading is necessary to construct the architecture of bone and to maintain bone mass. Mechanical strain plays an important role in bone remodeling during growth and development. Several studies indicate that osteblastic cells respond to physical loading by transducing signals that alter gene expression patterns. However, the cellular gene expression that controls the tissue response to mechanical force remains unclear. To identify genes that may be affected by the compression force, we used a cyclic compression force applied to MG63 osteoblast-like cell in 3D collagen gel. We then performed a cDNA microarray analysis on an array contain 7680 gene-specific DNA fragments. After normalization, 43 genes had significant expression, including many cellular genes that involved in apoptosis, cell cycle regulator, cell proliferation, metabolism, inflammation and other cellular function were observed. Some selected genes were chosen for further study by RT-PCR analysis. Our results indicate that the expression of Cyclooxygenase-2 (COX-2), Ornithine Decarboxylase (ODC), Matrix metalloproteinase-3 and -10 (MMP-3 and MMP-10) was up regulated by compression force, whereas the expression of Endothelin-2 (ET-2), Membrane bound IL-1 receptor accessory protein (mIL-1RAcP) was down regulated, which were consistent with microarray data. We examined the protein expression of MMP-3 and MMP-10 by western blotting, and it showed that MMP-3 proteins were slightly elevated by compression force stimulation, whereas the expression of MMP-10 in compression group was similar to that of the control group. The protein expression of MMP-3 and MMP-10 was also detected by zymogram, and it showed that both proteins were slightly elevated by compression force stimulation. Our results showered that compression force induces COX-2 and MMP-3 mRNA expression, and MMP-3 protein level also increased. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T08:02:16Z (GMT). No. of bitstreams: 1 ntu-94-R91422020-1.pdf: 1087735 bytes, checksum: dae7499fa6e01892c0f7bd8a5386699e (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄……………………………………………………………………I
表次目錄………………………………………………………………II 圖次目錄………………………………………………………………III 中文摘要………………………………………………………………IV 英文摘要………………………………………………………………V 第一章 引言……………………………………………………………1 第二章 實驗目的………………………………………………………15 第三章 實驗材料與方法………………………………………………16 第四章 結果……………………………………………………………24 第五章 討論……………………………………………………………28 第六章 結論……………………………………………………………40 表列……………………………………………………………………42 圖列……………………………………………………………………57 參考文獻………………………………………………………………68 表次目錄 表 1、半定量反轉錄-聚合酶鏈鎖反應各基因之正反譯引子、產物大小及黏合溫度…………………………………………………………42 表 2、過去文獻關於成骨母細胞受到壓力刺激的研究…………………………43 表 3、基因微陣列實驗的信賴度統計分析………………………………………46 表 4、未經標準化,基因微陣列分析顯示有顯著表現差異的84個基因及其表現數值………………………………………………………47 表 5、經標準化後30個受到活化的上調基因列表…………………52 表 6、經標準化後13個受到抑制之下調基因列表…………………54 表 7、以即時定量聚合酶連鎖反應檢測,壓力組與控制組COX-2與MMP-3mRNA的變化………………………………………………………55 表 8、以即時定量聚合酶連鎖反應檢測,壓力組與控制組MMP-10 mRNA的變化……………………………………………………………56 表 9、以即時定量聚合酶連鎖反應檢測,張力組與控制組COX-2,MMP-3與MMP-10 mRNA的變化…………………………………………57 圖次目錄 圖一、在不同血清濃度下,以週期性壓力刺激24小時後,對MG63細胞凋亡的影響………………………………………………………58 圖二、細胞壓力培養系統示意圖……………………………………59 圖三、未經標準化時,有顯著表現差異的84個基因依基因功能分類示意圖………………………60 圖四、經標準化後,30個上調基因與13個下調基因依基因功能分類示意圖………………………61 圖五、以半定量反轉錄-聚合酶連鎖反應檢測COX-2與ODC mRNA的變化……………………………62 圖六、以半定量反轉錄-聚合酶連鎖反應檢測MMP-3與MMP-10 mRNA的變化………………………………63 圖七、以半定量反轉錄-聚合酶連鎖反應檢測ET-2,sIL-1RAcP與mIL-1RAcP mRNA的變化………………………………………64 圖八、以半定量反轉錄-聚合酶連鎖反應分析COX-2,ODC,MMP-3,MMP-10,ET-2,sIL-1RAcP與mIL-1RAcP mRNA,壓力組與控制組比較示意圖……………………………………………………………65 圖九、週期性壓力刺激MG63細胞24小時後,以西方點墨法檢測細胞MMP-3蛋白質的表現量………………………………………………………….......66 圖十、週期性壓力刺激MG63細胞24小時後,以西方點墨法檢測細胞內MMP-10蛋白質的表現量………………………………………………………67 圖十一、週期性壓力刺激MG63細胞24小時後,以酵素活性分析法分析MMP-3與MMP-10分解gelatin的活性…………………………………………68 | |
dc.language.iso | zh-TW | |
dc.title | 週期性壓力刺激對類成骨母細胞基因表現的影響 | zh_TW |
dc.title | Changes of gene expression in MG63 cell under cyclic compression force stimulation | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林思洸,游偉絢 | |
dc.subject.keyword | 基因微陣列,成骨母細胞,壓力,間質分解酵素-3, | zh_TW |
dc.subject.keyword | microarray,osteoblast,compression force,matrix metalloproteinase-3, | en |
dc.relation.page | 80 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-22 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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