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請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36352
完整後設資料紀錄
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dc.contributor.advisor施秀惠(Hsiu-Hui Shih)
dc.contributor.authorYi-Ying Chouen
dc.contributor.author周宜瑩zh_TW
dc.date.accessioned2021-06-13T07:57:57Z-
dc.date.available2008-07-28
dc.date.copyright2005-07-28
dc.date.issued2005
dc.date.submitted2005-07-23
dc.identifier.citation朱祥海 編著(1997),魚類學。水產出版社。
沈世傑 主編(1993),臺灣魚類誌。國立臺灣大學動物學系印行。
吳宗穆(1996),臺灣近海鯨類之內寄生蟲相與生態關係之研究。國立臺灣大學獸醫研究所 碩士論文。
吳孟才(1997),臺灣經濟性海水魚類和鯨目動物內寄生蟲蟲相調查。國立臺灣大學獸醫學研究所 碩士論文。
查詩婷(2004),安尼線蟲科圓蟲與白帶魚之寄生關係。國立臺灣大學動物學研究所 碩士論文。
能勢幸雄、石井丈夫、清水誠 著;黃士宗、郭慶老 譯(1988),水產資源學。東京大學出版會。
施秀惠(2001),概說海獸胃線蟲。臺大漁推,第13期,1~13頁。臺灣大學漁業推廣委員會出版。
施秀惠(2004),海魚線蟲之迷思與剖析。臺大漁推,第15期,15~34頁。臺灣大學漁業推廣委員會出版。
曾長權(2001),聞魚色變-談環境風險認知。人本教育電子報。
劉明德(1998),市售海魚之海獸胃線蟲幼蟲之調查及其生物學之研究。國立臺灣大學醫學院微生物學研究所 寄生蟲學組 碩士論文。
潘炯華、張劍英、黎振昌(1990),魚類寄生蟲學。科學出版社。
戴昌鳳 編著(2004),台灣的海洋。遠足文化企業股份有限公司。
魏良佑(2003),臺灣東北部海域花腹鯖生殖生態學之初步研究。國立臺灣海洋大學環境生物與漁業科學學系 碩士論文。
Abollo E, Paggi L, Pascual S and Amelio SD’ (2003). Occurrence of recombinant genotypes of Anisakis simplex s. s. and Anisakis pegreffii (Nematoda:Anisakidae) in an area of sympatry. Infection, Genetics and Evolution 3, 175-181.
Aguado FG, Picazo A, Caballero ML, Moneo I, Asturias JA, Corcuera MT, Casado I and Alonso MJ (2003). Ultrastructural localization of Ani s 1, a major allergen from the fish parasite Anisakis simplex. Parasitology Research 89, 379-380.
Akao N, Ohyama TA and Kondo K (1990). Immunoblot analysis of serum IgG, IgA and IgE responses against larval excretory-secretory antigens of Anisakis simplex in patients with gastric anisakiasis. Journal of Helminthology 64, 310-318.
Amelio SD’, Mathiopoulos KD, Santos CP, Pugachev ON, Webb SC, Picanco M and Paggi L (2000). Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda:Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. International Journal of Parasitology 30, 223-226.
Audicana MT, Ansotegui IJ, de Corres LF and Kennedy MW (2002). Anisakis simplex:dangerous-dead and alive? Trends in Parasitology 18, 20-25.
Bouree P, Paugam A and Petithory JC (1995). Anisakidosis:Report of 25 cases and review of the literature. Comparative Immunology, Microbiology and Infectious Diseases 18, 75-84.
Bush AO, Lafferty KD, Lotz JM and Shostak AW (1997). Parasitology meets ecology on its own terms:Margolis et al. revisited. The Journal for Parasitology 83(4), 575-583.
Burton MB (1978). Population genetics of Anisakis simplex (Nematoda:Ascaridoidea) in Atlantic salmon (Salmo salar) and their use as biological indicators of host stocks. Environmental Biology of Fishes 3(4), 369-377.
Chang KH and Chen CP (1976). The stock discrimination and recruitmental age of spotted mackerel, Scomber australasicus in Taiwan. Bulletin of the Institute of Zoology, Academia Sinica 15(2), 57-64.
Chang KH and Wu WL (1977). Tagging experiments on the spotted mackerel (Scomber australasicus) in Taiwan. Bulletin of the Institute of Zoology, Academia Sinica 16(2), 137-139.
Cremonte F and Sardella NH (1997). The parasito fauna of Scomber japonicus Houttuyn, 1782 (Pisces:Scombridae) in two zones of the Argentine sea. Fisheries Research 31, 1-9.
Gasser RB and Monti JR (1997). Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA. Molecular and Cellular Probes 11, 201-209.
Grabda J (1974). The dynamics of the nematode larvae, Anisakis simplex (Rud.) invasion in the South-Western Baltic herring (Clupea harengas L.). Acta Ichthyologica Et Piscatoria 4, 3-21.
Hays R, Measures LN and Huot J (1998). Capelin (Mallotus villosus) and herring (Clupea harengus) as paratenic hosts of Anisakis simplex, a parasite of beluga (Delphinapterus leucas) in the St. Lawrence estuary. Canadian Journal of Zoology 76, 1411-1417.
Hayward CJ, Perera KML and Rohde K (1998). Assemblages of ectoparasites of a pelagic fish, slimy mackerel (Scomber australasicus), from South- Eastern Australia. International Journal for Parasitology 28, 263-273.
Højgaard DP (1999). No significant development of Anisakis simplex (Nematoda, Anisakidae) eggs in the intestine of long-finned pilot whales, Globicephala melas (Traill, 1809). Sarsia 84, 479-482.
Iglesias L, Valero A, Benítez R and Adroher FJ (2001). In vitro cultivation of Anisakis simplex:pepsin increases survival and moulting from fourth larval to adult stage. Parasitology 123, 285-291.
Iglesias R, Leiro J, Ubeira FM, Santamarina MT, Navarette I and Sanmartin ML (1996). Antigenic cross-reactivity in mice between third-stage larvae of Anisakis simplex and other nematodes. Parasitology Research 82, 378-381.
Kenzie KM and Abaunza P (1998). Parasites as biological tags for stock discrimination of marine fish:a guide to procedures and methods. Fisheries Research 38, 45-56.
Kijewska A, Slomiñska M, Wegrzyn G and Rokicki J (2000). A PCR-RFLP assay for identification of Anisakis simplex from different geographical regions. Molecular and Cellular Probes 14, 349-354.
Kijewska A, Rokicki J, Sitko J and Wegrzyn G (2002). Ascaridoidea:a simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds. Experimental Parasitology 101, 35-39.
Klimpel S, Palm HW, Rückert S and Piatkowski U (2004). The life cycle of Anisakis simplex in the Norwegian Deep (northern North Sea). Parasitology Research 94, 1-9.
Ku JF and Tzeng WN (1985). Age and growth of spotted mackerel, Scomber australasicus (Cuvier), in the shelf waters of northeastern and southwestern Taiwan. Journal of the Fisheries Society of Taiwan 12(2), 12-26.
Ku JF and Tzeng WN (1994). Age structure of spotted mackerel, Scomber australasicus, in the coastal waters of Taiwan as estimated from polymodal length-frequency analysis. Journal of the Fisheries Society of Taiwan 21(2), 121-137.
Ma HW, Jiang TJ, Quan FS, Chen XG, Wang HD, Zhang YS, Cui MS, Zhi WY and Jiang DC (1997). The infection status of anisakid larvae in marine fish and cephalopods from the Bohai Sea, China and their taxonomical consideration. The Korean Journal of Parasitology 35(1), 19-24.
Margolis L, Esch GW, Holmes JC, Kuris AM and Schad GA (1982). The use of ecological terms in parasitology (report of an ad hoc committee of the American society of parasitologists). The Journal for Parasitology 68(1), 131-133.
Mattiucci S, Nascetti G, Bullini L, Orecchia P and Paggi L (1986). Genetic structure of Anisakis physeteris, and its differentiation from the Anisakis simplex complex (Ascaridia:Anisakidae). Parasitology 93, 383-387.
Mattiucci S, Nascetti G, Cianchi R, Paggi L, Arduino P, Margolis L, Brattey J, Webb S, Amelio SD’, Orecchia P and Bullini L (1997). Genetic and ecological data on the Anisakis simplex complex with evidence for a new species (Nematoda Ascaridoidea, Anisakidae). The Journal of Parasitology 83, 401-416.
Mattiucci S, Paggi L, Nascetti G, Abollo E, Webb SC, Pascual S, Cianchi R and Bullini L (2001). Genetic divergence and reproductive isolation between Anisakis brevipiculata and Anisakis physeteris (Nematoda:Anisakidae)s. International Journal for Parasitology 31, 9-14.
Mattiucci S, Paggi L, Nascetti G, Santos CP, Costa G, Beneditto APD, Ramos R, Argyrou M, Cianchi R and Bullini L (2002). Genetic markers in the study of Anisakis typica (Diesing, 1860):larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda:Anisakidae). Systematic Parasitology 51, 159-170.
Moser M and Hsieh J (1992). Biological tags for stock separation in Pacific herring Clupea harengus pallasi in California. The Journal of Parasitology 78(1), 54-60.
Myers BJ (1976). Research then and now on the Anisakis nematodes. Trans actions of the American Microscopical Society 95, 137-142.
Nascetti G, Paggi L, Orecchia P, Smith JW, Mattiucci S and Bullini L (1986). Electrophoretic studies on the Anisakis simplex complex (Ascaridida:Anisakidae) from the Mediterranean and North-East Atlantic. International Journal for Parasitology 16, 633-640.
Nadler SA, Amelio SD’, Fagerholm H-P, Berland B and Paggi L (2000). Phylogenetic relationships among species of Contracaecum Railliet Henry, 1912 and Phocascaris Høst, 1932 (Nematoda:Ascaridoidea) based on nuclear rDNA sequence data. Parasitology 121, 455-463.
Orecchia P, Paggi L, Mattiucci S, Smith JW, Nascetti G and Bullini L (1986). Electrophoretic identification of larvae and adults of Anisakis (Ascaridia:Anisakidae). Journal of Helminthology 60, 331-339.
Paggi L, Nascetti G, Webb SC, Mattiucci S, Cianchi R and Bullini L (1998). A new species of Anisakis Dujardin, 1845 (Nematoda, Anisakidae) from beaked whales (Ziphiidae):allozyme and morphological evidence. Systematic Parasitology 40, 161-174.
Pampillón JAC, Búa MS, Domínguez HR, García JM, Fernández CA and Estévez JMG (2002). Selecting parasites for use in biological tagging of the Atlantic swordfish (Xiphias gladius). Fisheries Research 59, 259-262.
Pozdnyakov SE and Vasilenko AV (1994). Distribution, migration, and the helminth fauna of the Japanese mackerel, Scomber japonicus, in the Northwestern Pacific Ocean. Journal of Ichthyology 34(4), 74-91.
Rodero M, Jimenez A, Chivato T, Laguna R and Cuellar C (2001). Purification of Anisakis simplex antigen by affinity chromatography. Parasitology Research 87, 736-740.
Shih HH (2004). Parasitic helminth fauna of the cutlass fish, Trichiurus lepturus L., and the differentiation of four anisakid nematode third-stage larvae by nuclear ribosomal DNA sequences. Parasitology Research 93, 188-195.
Smith JW (1983). Anisakis simplex (Rudolphi, 1809, det. Krabbe, 1878) (Nematoda:Ascaridoidea):Morphology and morphometry of larvae from euphausiids and fish, and a review of the life-history and ecology. Journal of Helminthology 53, 205-224.
Smith JW (1984). The abundance of Aniskis simplex L3 in the body-cavity and flesh of marine teleosts. International Journal of Parasitology 14, 491-495.
Smith JW and Wootten R (1975). Experimental studies on the Migration of Anisakis sp. Larvae (Nematoda:Ascaridida) into the flesh of Herring, Clupea harengus L. International Journal for Parasitology 5, 133-136.
Smith JW and Wootten R (1978). Anisakis and Anisakiasis. Advances in Parasitology 16, 93-163.
Strømnes E and Andersen K (2000). “Spring rise” of whaleworm (Anisakis simplex;Nematoda, Ascaridoidea) third-stage larvae in some fish species from Norwegian waters. Parasitology Research 86, 619-624.
Strømnes E and Andersen K (2003). Growth of whaleworm (Anisakis simplex, Nematodes, Ascaridoidea, Anisakidae) third-stage larvae in paratenic fish hosts. Parasitology Research 89, 335-341.
Tzeng TD and Yeh SY (2002). Stock structure of spotted mackerel (Scomber australasicus) in Taiwan inferred from morphometric variation. Journal of the Fisheries Society of Taiwan 29(2), 117-128.
Tzeng TD (2004). Morphological variation between populations of spotted mackerel (Scomber australasicus) off Taiwan. Fisheries Research 68, 45-55.
Ugland KI, Strømnes E, Berland B and Aspholm PE (2004). Growth, fecundity and sex ratio of adult whaleworm (Anisakis simplex;Nematoda, Ascaridoidea, Anisakidae) in three whale species from the North-East Atlantic. Parasitology Research 92, 484-489.
Wang SB and Chen CT (1989). Reproductive periodictity and gonadal development of the Japenese butter fish, Psenopsis anomala. Bulletin of the Institute of Zoology, Academia Sinica 28(3), 225-235.
Wootten R and Waddell IF (1977). Studies on the biology of larval nematodes from the musculature of cod and whiting in Scottish waters. Journal Du Conseil/ Conseil International Pour l’exploration De La Mer 37(3), 266-273.
Zhu X, Gasser RB, Podolska M and Chilton NB (1998). Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology 28, 1911-1921.
Zhu X, Amelio SD’, Paggi L and Gasser RB (2000). Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda:Ascaridoidea:Annisakidae). Parasitology Research 86, 677-683.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/36352-
dc.description.abstract經濟性海水魚類感染海獸胃線蟲(Anisakis simplex)常有所聞,人們因攝食含有其第三期幼蟲之漁產品,而成為海獸胃線蟲的偶發性中寄主,以致罹患海獸胃線蟲症(anisakidosis)。此外,近年來研究指出,即使攝入為死蟲,亦可能引起胃過敏性海獸胃線蟲症(gastroallergic anisakidosis),導致發生胃腸不適與過敏症狀。本研究旨在查明花腹鯖之內寄生蟲相,統計其主要蟲種之感染情況,並選擇理想蟲種作為生物性標籤,藉以區分分佈於臺灣周圍海域之花腹鯖系群。
從南方澳漁港,連續採集一年之花腹鯖樣本(2004年4月至2005年3月),查明其內寄生蟲相發現主要以安尼線蟲科(Anisakisdae)線蟲為主,包括海獸胃線蟲(A. simplex)、有鉤宮脂線蟲(Hysterothylacium aduncum)、帶魚針蛔線蟲(Raphidascaris trichiuri)和前盲囊線蟲(Porrocaecum decipiens),在數量上以海獸胃線蟲第三期幼蟲為優勢蟲種( <0.01)。魚體內之海獸胃線蟲皆為第三期幼蟲,鑽孔齒和一個很大的胃是其特徵,分佈位置多位於體腔、肝臟和腸繫膜表面,多為自由活動之狀態,且具有很強的活動力;根據採樣月份、魚體體長和魚齡分組後,分析花腹鯖體內海獸胃線蟲第三期幼蟲的各項感染參數,包括:盛行率(prevalence)、感染強度(intensity)、平均感染強度(mean intensity)和豐富度(abundance)。結果發現在春季(3~5月)或體長大於280 mm或2歲以上的花腹鯖體內,才有高於100條幼蟲的感染,在生殖季節時花腹鯖體內幼蟲的感染強度亦高於非生殖季節的( <0.05)。
  Anisakis屬下共有7種,分別為A. pegreffii、A. simplex sensu stricto、A. simplex C、A. physeteris、A. schuppakovi、A. typica和A. ziphidarum等。在臺灣東北和西南海域中,至今已確認至少存有2個系群的花腹鯖,本研究利用PCR-RFLP方法分析採集自此二海域花腹鯖體內Anisakis屬線蟲第三期幼蟲,發現東北海域中存在A. pegreffii和重組基因型(A. pegreffii與A. simplex s. s.二種之的重組型),而西南海域中存有三種,除與前述東北海域相同二種外,另有A. typica存在。因此本研究發現Anisakis屬線蟲第三期幼蟲可作為生物性標籤,藉以區分臺灣周圍海域中花腹鯖之系群。
zh_TW
dc.description.abstractThe most principal nematode infected by economic fish was Anisakis simplex. People might become an accidental host of A. simplex third-stage larvae by ingestion of parasitized raw or undercooked fish. Whether dead or alive, these larvae might cause Anisakidosis or Gastroallergic anisakiasis. And they also induced digestive diseases or hypersensitivity reactions. The aim of this study is to investigate the parasitical fauna of spotted mackerel, Scomber australasicus, and then select an available parasite as the biological tag in stock identification of spotted mackerel around Taiwanese waters.
Spotted mackerel was sampled from Nan Fang Ao fishing port between April 2004 to March 2005. The main parasite fauna of spotted mackerel consists of nematodes belonging to the Family Anisakidae containing A. simplex, Hysterothylacium aduncum, Raphidascaris trichiuri, and Porrocaecum decipiens. And there was significant difference in numbers among A. simplex and the others ( <0.01). All A. simplex in fish were in the third-stage larval stage, a boring tooth and a big ventriculus were its characterization. These larvae were active and free in the body cavity which was their major location inside fish. In addition, encapsulated larvae were observed on livers and mesenteries.
All fish samples examined were grouped according to the sampling month, the body length (fork length) and age. An intensity more than 100 A. simplex third-stage larvae appeared in spring samples, the fork length of fish great than 280 mm, and the age more than 2 years old. The intensity of larvae infection was also higher in samples collected in reproduction season than that from non-reproduction season.
Two stocks of spotted mackerel of Taiwan named the Eastern-Northern group and the Western-Southern group was previously reported. Analysing the Anisakis populations inside spotted mackerel with PCR-RFLP, A. pegreffii and a recombinant genotype of A. simplex s. s. and A. pegreffii were found in the Eastern-Northern group. Besides these two species, a third species of A. typica was appeared in the Western-Southern group. It seem that the Anisakis larvae were useful biological tags in stock identification of spotted mackerel from Taiwanese waters.
en
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en
dc.description.tableofcontents目錄
頁碼
表次---------------------------------------------------------------------------------------- Ⅲ
圖次---------------------------------------------------------------------------------------- Ⅳ
中文摘要---------------------------------------------------------------------------------- Ⅴ
英文摘要---------------------------------------------------------------------------------- Ⅵ
第一章 前言---------------------------------------------------------------------------- 01
    1.寄生關係與線蟲感染魚類之歷史-------------------------------------- 01
    2.概述安尼線蟲科-Anisakis屬------------------------------------------ 02
    3.海獸胃線蟲(A. simplex)之生活史--------------------------------------- 04
    4.海獸胃線蟲對人體之影響----------------------------------------------- 06
    5.寄生蟲的鑑定-------------------------------------------------------------- 08
    6.臺灣漁業概述與魚類之重要性----------------------------------------- 09
    7.鯖魚(mackerel)的簡介---------------------------------------------------- 10
    8.花腹鯖系群(stock)的區分------------------------------------------------ 13
    9.研究目的-------------------------------------------------------------------- 15
第二章 材料與方法------------------------------------------------------------------- 16
    1.樣品的採集----------------------------------------------------------------- 16
    2.樣品的處理----------------------------------------------------------------- 16
    3.安尼線蟲科線蟲的分類-------------------------------------------------- 17
    4.花腹鯖年齡及生殖腺指數的估算-------------------------------------- 21
    5.感染參數的分析----------------------------------------------------------- 22
    6.資料分析-------------------------------------------------------------------- 23
第三章 結果---------------------------------------------------------------------------- 24
    1.花腹鯖之寄生蟲相-------------------------------------------------------- 24
2.海獸胃線蟲第三期幼蟲感染花腹鯖各項感染參數之分析-------- 25
3.以分子生物學方法鑑別海獸胃線蟲第三期幼蟲-------------------- 27
4.花腹鯖系群之區分-------------------------------------------------------- 27
    頁碼
第四章 討論---------------------------------------------------------------------------- 29
    1.海獸胃線蟲的魚類中間寄主及其第三期幼蟲在魚體內之分布-- 29
    2.海獸胃線蟲第三期幼蟲之感染參數在採樣月份間之變化
與意義----------------------------------------------------------------------- 31
    3.海獸胃線蟲第三期幼蟲之感染參數在體長分組與魚齡分組間
之變化與意義------------------------------------------------------------- 33
    4.花腹鯖的生殖特性與海獸胃線蟲第三期幼蟲之感染關係------- - 34
    5.利用Anisakis屬線蟲進行臺灣周圍海域花腹鯖系群之區分----- 35
參考文獻---------------------------------------------------------------------------------- 37
附錄---------------------------------------------------------------------------------------- 61
dc.language.isozh-TW
dc.subject系群zh_TW
dc.subject海獸胃線蟲zh_TW
dc.subject花腹鯖zh_TW
dc.subject生物性標籤zh_TW
dc.subjectScomber australasicusen
dc.subjectstocken
dc.subjectbiological tagsen
dc.subjectAnisakis simplexen
dc.title海獸胃線蟲與花腹鯖之寄生關係及系群區分之應用zh_TW
dc.titleThe Parasitism of Anisakis simplex on Spotted Mackerel, Scomber australasicus, and Its Application on Stock Identificationen
dc.typeThesis
dc.date.schoolyear93-2
dc.description.degree碩士
dc.contributor.oralexamcommittee蘇霩靄(Guo-Ai Su),許建宗(Chien-Chung Hsu)
dc.subject.keyword海獸胃線蟲,花腹鯖,生物性標籤,系群,zh_TW
dc.subject.keywordAnisakis simplex,Scomber australasicus,biological tags,stock,en
dc.relation.page61
dc.rights.note有償授權
dc.date.accepted2005-07-24
dc.contributor.author-college生命科學院zh_TW
dc.contributor.author-dept動物學研究研究所zh_TW
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