請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35919完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 賈景山 | |
| dc.contributor.author | Shu-Hui Wu | en |
| dc.contributor.author | 吳淑惠 | zh_TW |
| dc.date.accessioned | 2021-06-13T07:48:13Z | - |
| dc.date.available | 2010-08-12 | |
| dc.date.copyright | 2005-08-12 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-26 | |
| dc.identifier.citation | 1. Aoki, H., T. Shiroza, M. Hayakawa, S. Sato, and H. K. Kuramitsu. 1986. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect. Immun. 53:587-594.
2. Baorto, D. M., Z. Gao, R. Malaviya, M. L. Dustin, M. A. van der, D. M. Lublin, and S. N. Abraham. 1997. Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic. Nature 389:636-639. 3. Bockmann, R., C. Dickneite, B. Middendorf, W. Goebel, and Z. Sokolovic. 1996. Specific binding of the Listeria monocytogenes transcriptional regulator PrfA to target sequences requires additional factor(s) and is influenced by iron. Mol. Microbiol. 22:643-653. 4. Burnette-Curley, D., V. Wells, H. Viscount, C. L. Munro, J. C. Fenno, P. Fives-Taylor, and F. L. Macrina. 1995. FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis. Infect. Immun. 63:4669-4674. 5. Chang, S. C., K. T. Luh, L. J. Deng, and W. C. Hsieh. 1987. Bacteriology of viridans streptococcal bacteremia. Zhonghua Min Guo. Wei Sheng Wu Ji. Mian. Yi. Xue. Za Zhi. 20:311-318. 6. Chia, J. S., C. Y. Yeh, and J. Y. Chen. 2000. Identification of a fibronectin binding protein from Streptococcus mutans. Infect. Immun. 68:1864-1870. 7. Clarke J.K. 1924. On the bacterial factor in the aetiology of dental caries. Br. J. Exp. Pathol. 5:141-47. 8. Deonarine, B., J. Lazar, M. V. Gill, and B. A. Cunha. 1997. Quadri-valvular endocarditis caused by Streptococcus mutans. Clin. Microbiol. Infect. 3:139-141. 9. Dhawan, V. K. 2002. Infective endocarditis in elderly patients. Clin. Infect. Dis. 34:806-812. 10. Douglas, C. W., J. Heath, K. K. Hampton, and F. E. Preston. 1993. Identity of viridans streptococci isolated from cases of infective endocarditis. J. Med. Microbiol. 39:179-182. 11. Durack, D. T. 1995. Prevention of infective endocarditis. N. Engl. J. Med. 332:38-44. 12. Gahring, L. C., F. Heffron, B. B. Finlay, and S. Falkow. 1990. Invasion and replication of Salmonella typhimurium in animal cells. Infect. Immun. 58:443-448. 13. Germaine, G. R., S. K. Harlander, W. L. Leung, and C. F. Schachtele. 1977. Streptococcus mutans dextransucrase: functioning of primer dextran and endogenous dextranase in water-soluble and water-insoluble glucan synthesis. Infect. Immun. 16:637-648. 14. Gerondakis, S., M. Grossmann, Y. Nakamura, T. Pohl, and R. Grumont. 1999. Genetic approaches in mice to understand Rel/NF-kappaB and IkappaB function: transgenics and knockouts. Oncogene 18:6888-6895. 15. Gold, O. G., H. V. Jordan, and H. J. Van. 1973. A selective medium for Streptococcus mutans. Arch. Oral Biol. 18:1357-1364. 16. Gould, K., C. H. Ramirez-Ronda, R. K. Holmes, and J. P. Sanford. 1975. Adherence of bacteria to heart valves in vitro. J. Clin. Invest 56:1364-1370. 17. Hamill, R. J. 1987. Role of fibronectin in infective endocarditis. Rev. Infect. Dis. 9 Suppl 4:S360-S371. 18. Hanada, N. and H. K. Kuramitsu. 1989. Isolation and characterization of the Streptococcus mutans gtfD gene, coding for primer-dependent soluble glucan synthesis. Infect. Immun. 57:2079-2085. 19. Heithoff, D. M., C. P. Conner, P. C. Hanna, S. M. Julio, U. Hentschel, and M. J. Mahan. 1997. Bacterial infection as assessed by in vivo gene expression. Proc. Natl. Acad. Sci. U. S. A 94:934-939. 20. Herzberg, M. C. and M. W. Meyer. 1996. Effects of oral flora on platelets: possible consequences in cardiovascular disease. J. Periodontol. 67:1138-1142. 21. Kili, A. O., M. C. Herzberg, M. W. Meyer, X. Zhao, and L. Tao. 1999. Streptococcal reporter gene-fusion vector for identification of in vivo expressed genes. Plasmid 42:67-72. 22. Kuramitsu, H. K. and L. Wondrack. 1983. Insoluble glucan synthesis by Streptococcus mutans serotype c strains. Infect. Immun. 42:763-770. 23. Lawrence, T., D. W. Gilroy, P. R. Colville-Nash, and D. A. Willoughby. 2001. Possible new role for NF-kappaB in the resolution of inflammation. Nat. Med. 7:1291-1297. 24. Loesche, W. J. 1986. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50:353-380. 25. Lowe, A. M., D. T. Beattie, and R. L. Deresiewicz. 1998. Identification of novel staphylococcal virulence genes by in vivo expression technology. Mol. Microbiol. 27:967-976. 26. Lowrance, J. H., L. M. Baddour, and W. A. Simpson. 1990. The role of fibronectin binding in the rat model of experimental endocarditis caused by Streptococcus sanguis. J. Clin. Invest 86:7-13. 27. Mahan, M. J., J. M. Slauch, P. C. Hanna, A. Camilli, J. W. Tobias, M. K. Waldor, and J. J. Mekalanos. 1993. Selection for bacterial genes that are specifically induced in host tissues: the hunt for virulence factors. Infect. Agents Dis. 2:263-268. 28. Maurelli, A. T. 1989. Temperature regulation of virulence genes in pathogenic bacteria: a general strategy for human pathogens? Microb. Pathog. 7:1-10. 29. McNab, R., A. R. Holmes, J. M. Clarke, G. W. Tannock, and H. F. Jenkinson. 1996. Cell surface polypeptide CshA mediates binding of Streptococcus gordonii to other oral bacteria and to immobilized fibronectin. Infect. Immun. 64:4204-4210. 30. Miagkov, A. V., D. V. Kovalenko, C. E. Brown, J. R. Didsbury, J. P. Cogswell, S. A. Stimpson, A. S. Baldwin, and S. S. Makarov. 1998. NF-kappaB activation provides the potential link between inflammation and hyperplasia in the arthritic joint. Proc. Natl. Acad. Sci. U. S. A 95:13859-13864. 31. Moreillon, P. and Y. A. Que. 2004. Infective endocarditis. Lancet 363:139-149. 32. Olson, E. R. 1993. Influence of pH on bacterial gene expression. Mol. Microbiol. 8:5-14. 33. Pahl, H. L. 1999. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene 18:6853-6866. 34. Ross, R. 1999. Atherosclerosis--an inflammatory disease. N. Engl. J. Med. 340:115-126. 35. Scheld, W. M., J. A. Valone, and M. A. Sande. 1978. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J. Clin. Invest 61:1394-1404. 36. Shun, C. T., S. Y. Lu, C. Y. Yeh, C. P. Chiang, J. S. Chia, and J. Y. Chen. 2005. Glucosyltransferases of viridans streptococci are modulins of interleukin-6 induction in infective endocarditis. Infect. Immun. 73:3261-3270. 37. Teng, L. J., P. R. Hsueh, Y. C. Chen, S. W. Ho, and K. T. Luh. 1998. Antimicrobial susceptibility of viridans group streptococci in Taiwan with an emphasis on the high rates of resistance to penicillin and macrolides in Streptococcus oralis. J. Antimicrob. Chemother. 41:621-627. 38. Tompkins, D. C., V. B. Hatcher, D. Patel, G. A. Orr, L. L. Higgins, and F. D. Lowy. 1990. A human endothelial cell membrane protein that binds Staphylococcus aureus in vitro. J. Clin. Invest 85:1248-1254. 39. Ullman, R. F., S. J. Miller, M. J. Strampfer, and B. A. Cunha. 1988. Streptococcus mutans endocarditis: report of three cases and review of the literature. Heart Lung 17:209-212. 40. Valen, G., Z. Q. Yan, and G. K. Hansson. 2001. Nuclear factor kappa-B and the heart. J. Am. Coll. Cardiol. 38:307-314 41. Valen, G., G. Paulsson, and J. Vaage. 2001. Induction of inflammatory mediators during reperfusion of the human heart. Ann. Thorac. Surg. 71:226-232. 42. van Den Brink, G. R., F. J. ten Kate, C. Y. Ponsioen, M. M. Rive, G. N. Tytgat, S. J. van Deventer, and M. P. Peppelenbosch. 2000. Expression and activation of NF-kappa B in the antrum of the human stomach. J. Immunol. 164:3353-3359. 43. Viscount, H. B., C. L. Munro, D. Burnette-Curley, D. L. Peterson, and F. L. Macrina. 1997. Immunization with FimA protects against Streptococcus parasanguis endocarditis in rats. Infect. Immun. 65:994-1002. 44. Wells, V. D., C. L. Munro, M. C. Sulavik, D. B. Clewell, and F. L. Macrina. 1993. Infectivity of a glucan synthesis-defective mutant of Streptococcus gordonii (Challis) in a rat endocarditis model. FEMS Microbiol. Lett. 112:301-305. 45. Wilson, W. R., R. L. Thompson, C. J. Wilkowske, J. A. Washington, E. R. Giuliani, and J. E. Geraci. 1981. Short-term therapy for streptococcal infective endocarditis. Combined intramuscular administration of penicillin and streptomycin. JAMA 245:360-363. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35919 | - |
| dc.description.abstract | 轉糖鏈球菌(Streptococcus mutans)屬於草綠色鏈球菌，存在於人體口腔中並可引起齲齒。當轉糖鏈球菌進入血流中形成菌血症時，便有機會導致心臟瓣膜缺損的病人產生感染性心內膜炎（infective endocarditis, IE）。本實驗的第一部分中，為了找尋轉糖鏈球菌的新的毒性因子，我們建立一個適用於in vivo expression technology的表現載體，利用pDLDlacZ帶有兩個不具啟動子的報導基因：km-lac，而去篩選體內表現的基因。
本實驗第二部分是利用先前已建立好之實驗性心內膜炎之動物模型，探討S. mutans所引起心內膜炎的免疫反應。同時收集臨床人類心內膜炎檢體，觀察人類心內膜炎的慢性發炎的免疫反應特徵，並與實驗性心內膜炎相比較。實驗發現，造成感染的轉糖鏈球菌會存在於vegetation當中、纖維化的區域形成一小菌落，而避免受到白血球的吞噬作用。利用免疫染色發現，感染24小時後的巨噬細胞與纖維母細胞皆有NF-κB的活化。而在人類的組織切片中則可發現巨噬細胞、內皮細胞與纖維母細胞皆有細胞激素：IL-6、TNF-α與IL-1β的大量表現，並且伴隨著NF-κB的活化。因此我們推測纖維母細胞中的NF-κB活化在導致感染性心內膜炎持續性發炎中扮演一個重要的角色。 | zh_TW |
| dc.description.abstract | Streptococcus mutans, a member of the viridans streptococci, can cause dental caries in the oral cavity and infective endocarditis (IE) when entering into blood circulation. Major processes in the pathogenesis of IE are the formation of vegetations at the damaged surface on valves and inflammation of the endocardium. In the first part of this study, we developed a novel plasmid, pDLDlacZ for in vivo expression technology (IVET) in order to identify novel virulent factors of S. mutans. A Streptococcus-E. coli shuttle vector which carries dual (Km-lac) promoterless reporter genes has been constructed.
In the secondary part of the study, we used an experimental endocarditis rat model established previously in our laboratory to investigate the immune response induced by S. mutans. Human biopsies of infective endocarditis at chronic inflammatory stage was also analyzed and compared with the immune response identified on experimental endocarditis. Nests of S. mutans, with positive staining for secretory proteins, was walled-off in the fibrosis region of VG to avoid phagocytosis. Immunohistochemistry staining of the tissue sections showed that NF-κB was activated in both macrophage and fibroblast 24 hours after infection. In human biopsies, IL-6, TNF-α, and IL-1β secretion was found in macrophages, fibroblasts, and endothelium in addition to activated NF-κB. Therefore, NF-κB activation in the fibroblasts in endocardium might play an impartment role in the persistence of inflammation in IE | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T07:48:13Z (GMT). No. of bitstreams: 1 ntu-94-R92450012-1.pdf: 1908356 bytes, checksum: 69f3e6e135bad47e81623838bae73edd (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………1
英文摘要………………………………………………………………2 壹、緒論………………………………………………………………3 一、轉糖鏈球菌 …………………………………………………3 二、細菌的毒性因子………………………………………………5 三、感染性心內膜炎………………………………………………8 1.感染性心內膜炎的產生與臨床症狀 2.致病機轉 四、發炎反應………………………………………………………10 1.NF-κB與發炎反應的關係 2.感染性心內膜炎的發炎反應 五、實驗目的與設計………………………………………………14 貳、材料方法…………………………………………………………15 參、結果………………………………………………………………21 肆、討論………………………………………………………………28 圖表………………………………………………………………32 伍、參考文獻…………………………………………………………60 | |
| dc.language.iso | zh-TW | |
| dc.title | 篩選體內表現基因載體的建立與心內膜炎免疫反應之特徵 | zh_TW |
| dc.title | Construction of a plasmid for screening in vivo gene expression and characteristic of immune response in infective endocarditis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 江俊斌,郭彥彬,張百恩 | |
| dc.subject.keyword | 轉糖鏈球菌, | zh_TW |
| dc.subject.keyword | S. mutans,IVET,NF-kappa B, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-26 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 目前未授權公開取用 | 1.86 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。