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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 許圳塗(Chou-Tou Shii) | |
dc.contributor.author | Yu-Hsing Chien | en |
dc.contributor.author | 簡宇馨 | zh_TW |
dc.date.accessioned | 2021-06-13T07:07:49Z | - |
dc.date.available | 2005-07-28 | |
dc.date.copyright | 2005-07-28 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-27 | |
dc.identifier.citation | 江發讚、唐進. 1980. 百合科. 中國植物誌. 14篇. 科學出版社. 金石文. 1994. 百合屬之種源保存、遺傳歧異性分析及其種源利用與評估. 國立台灣大學園藝學研究所博士論文. 李俊賢. 1999. 百合LA及LO雜交種之組織培養及四倍體誘變. 國立台灣大學園藝學研究所碩士論文. 吳淑華. 2001. 基因型、滲透壓劑、ABA及Fluridone對百合微體繁殖小鱗莖葉片型態發育之影響. 國立台灣大學園藝學研究所碩士論文. 許圳塗. 1978. 台灣原生百合生長習性及栽培利用之研究. 國立台灣大學園藝系. 許圳塗、金石文、阮明淑. 2002. 百合. 實用花卉栽培技術專輯5. 陳孝銘. 2003. 外加Abscisic acid、Osmotica及Fluridone對百合組培苗小鱗莖葉片發育型之影響. 國立台灣大學園藝學研究所碩士論文. 曾美貞. 2000. 豔紅鹿子百合鱗片與癒傷組織培養之植株再生. 國立台灣大學園藝學研究所碩士論文. 黃學林、李筱菊. 2000. 植物體細胞胚胎發生. 科學農業. 48: 120-127. 董立. 1978. 球根花卉. 自然科學文化事業公司. 台北. 蔡淑華. 1988. 植物組織切片技術綱要. 茂昌股份有限公司. 台北. 劉蓁蓁. 1989. 亞洲型、鐵炮型和東方型百合群間雜交親和性及其遺傳標誌. 國立台灣大學園藝學研究所碩士論文. 62
簡瑋廷. 2001. 新鐵砲百合(Lilium × formolongi hort.)之體胚發生與大量繁殖. 大同大學生物工程研究所碩士論文. Ammirto, P. V. 1984. Induction, maintenance, and manipulation of development in embryogenic cell suspension cultures. In: Vasil, I. K. (ed). Cell Culture and Somatic Cell Genetics of Plants. Vol. I. Academic Press, Inc. London. p. 139-151. Arnold, S. V., I. Sabala, P. Bozhkov, J. Dyachok and L. Filonova. 2002. Development pathways of somatic embryogenesis. Plant Cell Tiss Org Cult. 69: 233-249. Arzate-Fernández, A. -M., T. Nakazaki, Y. Okumoto and T. Tanisaka. 1997. Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb.). Plant Cell Rep. 16: 836-840. Bacchetta, L., P. C. Remotti, C. Bernardini and F. Saccardo. 2003. Adventitious shoot regeneration from leaf explants and stem nodes of Lilium. Plant Cell Tiss Org Cult. 74: 37-44. Bong Hee, H., Y. Byeing Woo, G. Dae Hoe and K. Jae Young. 1999. The formation and growth of bulblets from bulblet sections with swollen proximal plate in Lilium oriental hybrid ‘Casa Blanca’. J. Kor. Soc. Hort. Sci. 40: 747-750. (Abs.) Dong-Sheng, H., N. Yoshiji and N. Masaru. 2000. Formation of calli from isolated microspore cultures of Asiatic hybrid lily ‘Connecticut King’. J. Jap. Soc. Hort. Sci. 69: 52-56. (Abs.) Eui, Young K., C. Jeong Doo, P. Kyeong II, B. Mi Soon and K. Kiu Weon. 1999. Enhancement of proliferation rate through multiple shoot induction from culture of microscale section in Lilium. J. Kor. Soc. Hort. Sci. 40: 459-462. (Abs.) Famelaer, I., E. Ennik, W. Eikelboom, J. M. Van Tuyl and J. Creemers-Molenaar. 1996. The initiation of callus and regeneration from callus culture of Tulipa gesneriana. Plant Cell Tiss Org Cult. 47: 51-58. Fernandez, A. M., T. Nakazaki and T. Tanisaka. 1996. Development of diploid and triploid interspecific hybrids between Lilium longiflorum and L. concolor by ovary slice culture. Plant Breed. 115: 167-171. Godo, T., K. Kobayashi, T. Tagami, K. Matsui and T. Kida. 1998. In vitro propagation utilizing suspension cultures of meristematic nodular cell clumps and chromosome stability of Lilium × foromolongi hort. Sci. Hortic. 72: 193-202. Haensch, K. -T. 1996. Plant regeneration through somatic embryogenesis in different genotypes of Lilium-hybrids. Gartenbauwissenschaft. 61: 214-218. Han, D., Y. Niimi and M. Nakano. 2000. Long term maintenance of anther-derived haploid callus line of the Asiatic hybrid lily ‘Connecticut King’. Plant Cell Tiss Org Cult. 61: 215-219. Hussey, G. 1975. Totipotency in tissue explants and callus of some members of the Lilaceae, Iridaceae, and Amaryllidaceae. J. Exp. Bot. 26: 253-262. Ikeda, N., Y. Niimi and D. -S. Han. 2003. Production of seedings from ovules excised at the zygote stages in Lilium spp. Plant Cell Tiss Org Cult. 73: 159-166. Jiménez, V. M. and F. Bangerth. 2001. Hormonal status of maize initial explants and of the embryogenic and non-embryogenic callus cultures derived from them as related to morphogenesis in vitro. Plant Sci. 160: 247-257. Kahane, R., B. Teyssendier and M. Rancillac. 1992. Bulbing in long-day Onion (Allium cepa L.) cultured in vitro: Compassion between sugar feeding and light induction. Ann. Bota. 69: 551-555. Kim, Y. -S., E. -J. Hahn and K. -Y. Paek. 2001. A large scale production of Lilium bulblets through bioreactor culture. Acta Hortic. 560: 383-386. Kyeung, II P., C. Jeong Doo, E. Sun Jung and K. Kiu Weon. 2002. Effects of MS medium strength, nitrogen concentration, culture temperature and daylength on callus proliferation and plant regeneration from calli of Lilium Oriental Hybrids ‘Casa Blanca’. J. Kor. Soc. Hort. Sci. 43: 628-632. (Abs.) Kyeung, II P., C. Jeong Doo, B. Mi Soon, C. Jong Jin, K. Kyeong Hak and K. Kiu Weon. 2000. Induction and proliferation of callus in Lilium Oriental Hybrids in vitro. J. Kor. Soc. Hort. Sci. 41: 641-646. (Abs.) Mann, J. D. 1983. Translocation of photosynthate in bulbing onions. Aust. J. Physiol. 10: 515-521. Mielke, K. A. and W. C. Anderson. 1989. In vitro bulblet formation in Dutch iris. Hort. Sci. 24: 1028-1031. Mii, M., Y. Yuzawa, H. Suetomi, T. Motegi and T. Godo. 1994. Fertile plant regeneration from protoplasts of a seed-propagated cultivar of Lilium × formolongi by utilizing meristematic nodular cell clumps. Plant Sci. 100: 221-226. Nakano, M., T. Sakakibara, S. Suzuki and H. Saito. 2000. Decrease in the regeneration potential of long-term cell suspension cultures of Lilium formosanum Wallace and its restoration by the auxin transport inhibitor, 2,3,5-triiodobenzoic acid. Plant Sci. 158: 129-137. Nhut, D. T., B. V. Le, N. T. Minh, J. T. de Silva, S. Fukai, M. Tanaka and K. T. T. Van. 2002. Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum. Plant Grow Reg. 37: 193-198. Nhut, D. T., B. V. Le, S. Fukai, M. Tanaka and T. T. Van. 2001. Effects of activated charcoal, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Grow Reg. 33: 69-65. Nishiwaki, M., K. Fujino, Y. Koda, K. Masuda and Y. Kikata. 2000. Somatic embryogenesis induced by the simple application of abscisic acids to carrots (Daucus carota L.) seedlings in culture. Planta. 211: 756-759. Okazaki, K. and M. Koizumi. 1995. Callus formation and regeneration of some species of Lilium. Acta Hortic. 392: 97-106. Okazaki, K., M. Koizumi, K. Masaaki and M. Kinji. 1995. Introduction of the characteristics of Lilium concolor into L. times ‘Asiatic Hybrids’ by crossing through style-cutting pollination and embryo culture. J. Jap. Soc. Hort. Sci. 63: 825-833. (Abs.) Pelkonen, V. and A. Kauppi. 1999. The effect of light and auxins on the regeneration of lily (Lilium regale Wil.) cells by somatic embryogenesis and organogenesis. Int. J. Plant Sci. 160: 483-490. Priyadarshi, S. and S. Sen. 1992. A revised scheme for mass propagation of Easter lily. Plant Cell Tiss Org Cult. 30: 193-197. Ramsay, J. L. and D. S. Galitz. 2003. Basal medium and sucrose concentration influence regeneration of Easter lily in ovary culture. HortSci. 38: 404-406. Sheridan, W. F. 1968. Tissue culture of the monocot Lilium. Planta. 82: 189-192. Shigeru, M. and O. Masanori. 1994. Effects of naphthaleneacetic acid and benzyladenine on growth of and formation of bulblets regenerated from white callus of mother scale of Lilium japonicum Thunb. J. Japn. Soc. Hort. Sci. 63: 429-437. (Abs.) Shigeru, M., O. Masanori and I. Tetsuro. 1994. Effects of naphthaleneacetic acid and benzyladenine on the growth of white callus and formation of bulblet from mother-scale of Lilium japonicum Thunb. J. Kor. Soc. Hort. Sci. 63: 131-137. (Abs.) Simmonds, J. A. and B. G. Cumming. 1976. Propagation of Lilium hybrids. II. Production of plantlets from bulb-scale callus cultures for increased propagations rates. Sci. Hortic. 5: 161-170. Tribulato, A., P. C. Remotti, H. J. M. Löffler and J. M. van Tuyl. 1997a. Occurrence of embryo like structures and plant regeneration from a cell suspension of Lilium longiflorum. Acta Hortic. 447: 205-206. Tribulato, A., P. C. Remotti, H. J. M. Löffler and J. M. van Tuyl. 1997b. Somatic embryogenesis and plant regeneration in Lilium longiflorum Thunb. Plant Cell Rep. 17: 113-118. Wickremesinhe, E. R. M., E. J. Holcomb and R. N. Arteca. 1994. A practical method for the production of flowing Easter lilies from callus cultures. Sci Hort. 60: 143-152. Williams, E. G. and G. Maheswaran. 1986. Somatic embryogenesis: Factors influencing coordinated behaviour of cells as an embryogenic group. Ann. Bot. 57: 443-462. Young Seok, J., K. Cheul Woo, K. Yong Beom, H. Dong Yun, C. In Hoo, J. Byeong Chun, P. Jong Hwan and P. Sun Hee. 2002. Regeneration of haploid plants through anther culture in Lilium longiflorum Thunb. ‘Georgia’. J. Kor. Soc. Hort. Sci. 43: 231-234. (Abs.) Zimmerman, J. L. 1993. Somatic embryogenesis: A model for early development in higher plants. Plant cell. 5: 1411-1423. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35743 | - |
dc.description.abstract | 試驗結果顯示將培植體鱗片進行分切處理,’Gel × PS’之鱗片基端癒合組織再生頻率最高,可達40%。完整鱗片之再生部位亦為靠近基盤位置。’Gel × PS’癒合組織再生位置多位於培植體基端,而’LD × CA’培植體頂端之形態發育多為不定芽及不定根之再生。將鱗片培植體四分切,由於培植體過小,誘導反應多為培植體稍微膨大,生長緩慢以及切口處褐化等現象,無法進行逆分化或誘導再生。
單一種類生長素之處理結果:Picloram對於癒合組織之誘導效果最佳,且隨著施用濃度增加而提高,’Gel × PS’、 ’LD × CA’、 ’Casa Blanca’最佳再生頻率分別為54%、70%、98%。2,4-D處理隨濃度提高,培植體褐化與畸形情形越嚴重,’Gel × PS’、 ’LD × CA’以2,4-D 5.0 mg.L-1處理分別產生88%、42%之褐化率。施用NAA容易誘導培植體分化多量不定根。處理Dicamba 1.0 – 2.0 mg.L-1對於癒合組織再生頻率較高,但培植體隨培養時間朝定向分化或褐化,導致癒合組織發生頻率降低。 處理組合以Picloram 0.5 mg.L-1、2,4-D 2.0 mg.L-1、以及kinetin 1.0 mg.L-1三種組合對於 ‘LD × CA’與’Casa Blanca’誘導癒合組織頻率較高,分別達38%與22%之誘導再生率。另以1.0 mg.L-1之Picloram與2,4-D組合zeatin 0.1 – 1.0 mg.L-1培養基進行誘導,三雜交品種對於2,4-D與zeatin之組合誘導不定芽再生頻率高,皆可達80%。2,4-D 1.0 mg.L-1組合zeatin 0.1 mg.L-1易誘導培植體形成不抽葉之白化叢生芽體,提高zeatin濃度會減少小鱗莖再生數目,但會抽葉。培植體對於Picloram與zeatin組合處理,較易朝向癒合組織之形成,但褐化與畸形情形也較嚴重。 添加不同濃度ABA於2,4-D 1.0 mg.L-1培養基,對‘LD × CA’誘導癒合組織再生頻率皆很低,培植體大多朝向不定芽與不定根之器官發生。低濃度ABA可促進鱗片葉之再生,而高濃度ABA處理誘導之小鱗莖較為膨大,但無抽葉現象。單獨施用ABA 2.5 mg.L-1之培植體之發根數與不定芽再生數皆降低,且生長緩慢。 在切片組織形態觀察方面,’Gel × PS’以Picloram組合2,4-D繼代培養,癒合組織表面呈現細胞小且細胞質稠密,相似於分生組織之構造,靠近中心之癒合組織則為液胞化之空心構造。 | zh_TW |
dc.description.abstract | Explants of ‘Gel × PS’ from proximal ends showed the highest rate of callus regeneration, and the frequency was 40%. Regeneration positions of intact mini-scale cultures were also approach to the basal plates. Oppositely, explants of ‘LD × CA’ from distal ends formed adventitious roots or shoots. As a result of explants cut into four were too small to induce responses towards swelling slightly, growth slowly, and browning in cut sites. Explants couldn’t be induced to dedifferentiation or regeneration neither.
Picloram was more effective for inducing callus than other kinds of auxin. The callus inducing efficiency went along with the concentrations. The highest frequency of callus formation respectively in ‘Gel × PS’, ‘LD × CA’, ‘Casa Blanca’ were 54%, 70%, and 98%. Explants with 2,4-D treatment showed an increased portion of brownish and malformed structures, and the browning frequency in ‘Gel × PS’ and ‘LD × CA’ were 88% and 42% in medium containing 5.0 mg.L-1 2,4-D. Multiple adventitious roots were stimulated by NAA. Explants treated with 2.0 mg.L-1 Dicamba were suitable for callus formation, but the frequency declined when cultured time passed. The explants could result in determinated differentiation or browning. More calli were formed well on MS medium supplemented with 0.5 mg.L-1 Picloram, 2.0 mg.L-1 2,4-D and 1 mg.L-1 kinetin for explants of ‘LD × CA’ and ‘Casa Blanca’, and the regeneration rates were 38% and 22%. Shoots formation efficiencies were high on MS medium supplemented with 2,4-D (1.0 mg.L-1) and zeatin (0.1 – 1.0 mg.L-1)for three hybrid lilies. The frequencies could be above 80%. Multiple albino shoots without leaves were induced on MS medium supplemented with 1.0 mg.L-1 2,4-D and 0.1 mg.L-1 zeatin. Raising the concentrations of zeatin would decline regeneration numbers of bulblets with leaves emergence. Explants cultured in Picloram and zeatin combination developed towards callus formation, but the brownish and malformed cases were as well as more serious. Explants of ‘LD × CA’ cultured on MS medium containing ABA (0.1 – 2.5 mg.L-1) and 2,4-D (1.0 mg.L-1) induced towards organogenesis, but callus induction frequency was low. Low concentration of ABA increased scale number, while high concentration of ABA promoted non-leaving bulbing. Addition 2.5 mg.L-1 ABA to medium showed the decline in number of roots and shoots regeneration and tardy growth for explants. Morphological and anatomic observations in ‘Gel × PS’ sub-cultured with Picloram and 2.4-D showed the surface of the callus cells were small and dense, and they resembled meristemic structures. Cells inside the callus observed vacuolated and hollow structures. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T07:07:49Z (GMT). No. of bitstreams: 1 ntu-94-R92628124-1.pdf: 1012317 bytes, checksum: b9dabdec0e46aae9208be8b7ac9bc011 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 前言(Introduction)………………………….…………………………...1
前人研究(Literature Review)…………………………………………...3 一、百合相關研究………………...………………………...………...3 二、影響百合癒合組織發生因子…...……………………..…………4 三、百合之遺傳穩定性………………...……………………………..9 四、癒合組織之形態……………………………………….………....9 材料與方法(Materials and Methods)……………….…………………11 一、參試材料………………………………….…..………...……….11 二、試驗方法…………………………...…...……………...………..11 (一)培植體培養………………………………………...…………11 (二)癒合組織誘導與器官發生.…………..……….…...…………11 1. 培植體處理...……………….………………………...….………11 2. 生長調節物質…………..………………………………..……...12 (1) 單一生長素……………………..………………………...…….12 (2) 複合生長素……………..………………………………...…….12 (3) 生長素組合細胞分裂素…………...……………………...……12 (4) ABA處理……………………………………………...……...….12 (三)小植株建立…………..…………………………………….…13 1. 培植體存活率調查………………………...…………………….13 2. 促進抽葉……………………………………….………………...13 3. 移植出瓶……………………………...…….……………………13 (四)石蠟切片分析……………..……………………...…………..13 結果(Results)……………………………………..……………………16 一、癒合組織誘導與器官發生………………...…...……..………...16 (一)培植體再生位置………………………...…………………....16 (二)生長調節物質…………………………...……………………16 1. 單一生長素…………………………………...………………….16 2. 複合生長素………………………………………...…………….17 3. 生長素組合細胞分裂素……………………………...………….17 4. ABA處理………………………………………………...…….…18 二、小植株建立再生………………………………………...………19 1. 培植體存活率調查………………...……………………...……..19 2. 促進抽葉………………………….………………………...……19 3. 移植出瓶…………………….………………………………...…19 三、 石蠟切片觀察…………………………………………………19 討論(Discussion)……………………………………………………….52 一、 癒合組織誘導與器官發生……………………………………52 1. 培植體位置…………………………………………………....52 2. 生長調節物質………………………………………………....53 (1) 單一生長素…………………………………………………......53 (2) 生長素組合細胞分裂素……………………………………......54 (3) ABA處理………………………………………………………...56 二、 小植株建立……………………………………………………57 摘要(Summary)………………………………………………………...58 參考文獻(References)…………………………………………………62 | |
dc.language.iso | zh-TW | |
dc.title | LA與LO型雜交種百合組織培養之癒合組織形成與植株再生 | zh_TW |
dc.title | Callus Formation and Regeneration in Tissue Culture of LA and LO Hybrid Lilies | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張唯勤(Wei-Chin Chang),李哖(Nean Lee) | |
dc.subject.keyword | 癒合組織,百合,小鱗片培養, | zh_TW |
dc.subject.keyword | callus,lily,mini-scale culture, | en |
dc.relation.page | 68 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-27 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝學研究所 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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