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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳益群(Yi-Chun Wu) | |
| dc.contributor.author | Ju-Ling Liu | en |
| dc.contributor.author | 劉如玲 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:56:35Z | - |
| dc.date.available | 2007-08-01 | |
| dc.date.copyright | 2005-08-01 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-27 | |
| dc.identifier.citation | References
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35520 | - |
| dc.description.abstract | 多細胞生物體中，TLK(tousled-like kinase)是一種保守的絲氨酸/蘇氨酸激酶家族，之前研究顯示，人類的TLK在細胞週期中S期作用於染色質的組成(chromatin assembly)，以及果蠅的TLK在M期TLKs作用於染色體的濃縮。最近研究顯示，線蟲TLK-1在轉錄上扮演著重要角色。我們則發現tlk-1 (RNAi)突變體停留在約五十五個細胞時期，並具有大小不一致分怖的細胞核，由於非整倍體表型(aneuploidy)所造成。我們利用GFP 標示tubulin和組蛋白(histone) 的轉殖基因(transgenes)，結果顯示tlk-1 (RNAi)突變體在有絲分裂時具有DNA濃縮和分離的缺失。我們另外發現著絲點蛋白(holocentric proteins) HCP-1/CENP-F和HCP-3/CENP-A在tlk-1 (RNAi)突變體的mitotic DNA有著不正常分佈。此外，在yeast two-hybrids實驗中，TLK-1可與HCP-3和HCP-4 /CENP-C結合。我們推論線蟲TLK-1控制HCP-1和HCP-3正確地排列在mitotic染色體的外側 (poleward face)，是藉由與HCP-3和HCP-4的結合，進而影響在有絲分裂中染色體的濃縮與分裂。 | zh_TW |
| dc.description.abstract | TLK, tousled-like kinase, is a family of conserved serine/ threonine kinase in multicellular organisms. Previous studies showed that human TLK functions in chromatin assembly at S phase and that Drosophila TLK acts in chromosome condensation at M phase during the cell cycle. Recent studies showed that C.elegans TLK-1 plays an essential role in transcription. We found that tlk-1 (RNAi) mutants arrested at about the 55-cell stage with uneven distribution of small and large nuclei due to aneuploidy. Using GFP to label tubulin and histone, we found that tlk-1 (RNAi) embryos have defects in DNA condensation and segregation during mitosis. We also found that holocentric proteins HCP-1/CENP-F and HCP-3/CENP-A are not properly localized to mitotic DNA in tlk-1 (RNAi) embryos. Furthermore, in the yeast-2 hybrid system TLK-1 interacts with HCP-3 and HCP-4/CENP-C. We propose that TLK-1 controls the proper localization of HCP-3 and HCP-1 to the poleward face of mitotic DNA, likely through direct interaction of TKL-1 with HCP-3 and HCP-4, and thereby functions in chromosome condensation and segregation during mitosis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:56:35Z (GMT). No. of bitstreams: 1 ntu-94-R92b43004-1.pdf: 2431951 bytes, checksum: 5ac44c8a0a10f3f819355f1568a06afd (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | Contents
Abstract…………………..……………………………………………4 中文摘要…………………………………………………………………5 Introduction………………..…………………………………………6 Materials and Methods…………………...…………….…………10 Caenorhabditis elegans Strains……………………..………...10 RNA mediated interference (RNAi)……………………………...10 DAPI staining …………………………………………….………..11 Time-lapse and z-series stacks microscopy……….………...11 Immunostaining……………………………………………....…….12 TOPOCloning………. …………………………………….....…….13 Gateway System…………………………………………….........14 Yeast two-hybrid assay……………………………….…....……14 Results………………………………………………..………………16 tlk-1 (RNAi) mutant causes the early embryonic lethality with aneuploidy phenotype…….………………….……………..16 tlk-1 (RNAi) mutant exhibited defects in chromosome condensation and segregation……….......….……………….17 C. elegans TLK-1 affects centromeric protein assembling on chromosome…………………….....………….………………....18 C. elegans holocentric protein does not affect the TLK-1 localization............................................19 C. elegans TLK-1 physically interacts with HCP-3 and HCP-4 in yeast two-hybrid system……………………..……………..20 Discussion……………………......………………………………21 Chromosome condensation and segregation defects in tlk-1 (RNAi)mutants……………….………………………………………21 C. elegans TLK-1 affects centromeric protein assembling on chromosome………………………………………………………....22 The possible phosphorylation site of HCP-3 and the function of the phosphorylation…….………………………..23 Figures and Tables………….…………………………………...24 References……………….………………………………………...36 | |
| dc.language.iso | en | |
| dc.title | 線蟲TLK-1影響染色體的著絲點組成 | zh_TW |
| dc.title | C. elegans TLK-1 Affects Holocentric Protein Assembly on Chromosome | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳蕾惠(Rey-Huei Chen),黃奇英(Chi-Ying Huang),廖國楨(Gwo-Jen Liaw) | |
| dc.subject.keyword | 著絲點, | zh_TW |
| dc.subject.keyword | TLK-1, | en |
| dc.relation.page | 39 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-28 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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