請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35396完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 龐飛(Victor Fei Pang) | |
| dc.contributor.author | Hue-Ying Chiou | en |
| dc.contributor.author | 邱慧英 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:51:01Z | - |
| dc.date.available | 2006-07-30 | |
| dc.date.copyright | 2005-07-30 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-28 | |
| dc.identifier.citation | 1. 林翰傑。2001。豬場工作人員、豬、鼠類及週遭動物鉤端螺旋體症血清流行病學調查暨利用台灣地區登革熱通報系統檢出陰性血清監測鉤端螺旋體症之發生。國立台灣大學獸醫學研究所碩士論文。
2. 高雅敏。1997。台灣地區豬與軍犬鉤端螺旋體症的血清流行病學調查。國立台灣大學獸醫學研究所碩士論文。 3. 1982, Guidelines for the control of leptospirosis. WHO Offset Publ, 1-171. 4. Adams, L.G., Polzin, D.J., Osborne, C.A., O'Brien, T.D., Hostetter, T.H., 1994, Influence of dietary protein/calorie intake on renal morphology and function in cats with 5/6 nephrectomy. Lab Invest 70, 347-357. 5. Adler, B, and Faine, S., 2002, The genus Leptospira. The prokaryotes: An evolving electronic resource for the microbiological community, 3rd edition, release 3.9, http://141.150.157.117:8080/prokPUB/chaprender /jsp/showchap.jsp?chapnum=311&initsec=01_00 6. Agunloye, C.A., Nash, A.S., 1996, Investigation of possible leptospiral infection in cats in Scotland. J Small Anim Pract 37, 126-129. 7. Alexander, A.D., 1984, Leptospirosis in laboratory mice. Science 224, 1158. 8. Anderson, D.C., Geistfeld, J.G., Maetz, H.M., Patton, C.M., Kaufmann, A.F., 1978, Leptospirosis in zoo workers associated with bears. Am J Trop Med Hyg 27, 210-211. 9. Artois, M., Claro, F., Remond, M., Blancou, J., 1996, [Infectious pathology of Canidae and Felidae in zoological parks]. Rev Sci Tech 15, 115-140. 10. Barkin, R.M., Guckian, J.C., Glosser, J.W., 1974, Infection by leptospira ballum: a laboratory-associated case. South Med J 67, 155 passim. 11. Bolton, L.A., Munson, L., 1999, Glomerulosclerosis in captive cheetahs (Acinonyx jubatus). Vet Pathol 36, 14-22. 12. Carlos, E.R., Kundin, W.D., Watten, R.H., Tsai, C.C., Irving, G.S., Carlos, E.T., Directo, A.C., 1971, Leptospirosis in the Philippines: feline studies. Am J Vet Res 32, 1455-1456. 13. Carmichael, K.P., Bienzle, D., McDonnell, J.J., 2002, Feline leukemia virus-associated myelopathy in cats. Vet Pathol 39, 536-545. 14. Citino, S.B., 1988, Use of a subunit feline leukemia virus vaccine in exotic cats. J Am Vet Med Assoc 192, 957-959. 15. Daniels, M.J., Golder, M.C., Jarrett, O., MacDonald, D.W., 1999, Feline viruses in wildcats from Scotland. J Wildl Dis 35, 121-124. 16. Deem, S.L., Heard, D.J., LaRock, R., 1998, Heartworm (Dirofilaria immitis) disease and glomerulonephritis in a black-footed cat (Felis nigripes). J Zoo Wildl Med 29, 199-202. 17. Dua, N., Reubel, G., Moore, P.F., Higgins, J., Pedersen, N.C., 1994, An experimental study of primary feline immunodeficiency virus infection in cats and a historical comparison to acute simian and human immunodeficiency virus diseases. Vet Immunol Immunopathol 43, 337-355. 18. Dubey, J.P., Lindsay, D.S., Lipscomb, T.P., 1990, Neosporosis in cats. Vet Pathol 27, 335-339. 19. Ellis, J.A., Jackson, M.L., Bartsch, R.C., McGill, L.G., Martin, K.M., Trask, B.R., Haines, D.M., 1996, Use of immunohistochemistry and polymerase chain reaction for detection of oncornaviruses in formalin-fixed, paraffin-embedded fibrosarcomas from cats. J Am Vet Med Assoc 209, 767-771. 20. Endo, Y., Uema, M., Miura, R., Tsukiyama-Kohara, K., Tsujimoto, H., Yoneda, K., Kai, C., 2004, Prevalence of canine distemper virus, feline immunodeficiency virus and feline leukemia virus in captive African lions (Panthera leo) in Japan. J Vet Med Sci 66, 1587-1589. 21. Ettinger, S.J., Feldman, E.C., 1995, Textbook of veterinary internal medicine. Saunders, pp. 1706-1860. 22. Faine, S., Adler, B., Bolin, C., Perolat, P., 1999, Leptospira and Leptospirosis. MediSci® 23. Fischer-Tenhagen, C., Hamblin, C., Quandt, S., Frolich, K., 2000, Serosurvey for selected infectious disease agents in free-ranging black and white rhinoceros in Africa. J Wildl Dis 36, 316-323. 24. Fox, M.J., Pilon, J.E., Jr., 1964, Leptospirosis: Case Report And Review Of Recent Literature. Wis Med J 63, 465-470. 25. Grauer, G.F., 1992, Glomerulonephritis. Semin Vet Med Surg (Small Anim) 7, 187-197. 26. Gravekamp, C., Van de Kemp, H., Franzen, M., Carrington, D., Schoone, G.J., Van Eys, G.J., Everard, C.O., Hartskeerl, R.A., Terpstra, W.J., 1993, Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J Gen Microbiol 139, 1691-1700. 27. Greenlee, J.J., Bolin, C.A., Alt, D.P., Cheville, N.F., Andreasen, C.B., 2004, Clinical and pathologic comparison of acute leptospirosis in dogs caused by two strains of Leptospira kirschneri serovar grippotyphosa. Am J Vet Res 65, 1100-1107. 28. Hamilton, J.M., Naylor, J., Weatherley, A., 1982, Glomerular lesions associated with infestation with Toxocara cati. Vet Rec 111, 583-584. 29. Hardy, W.D., Jr., Hess, P.W., Essex, M., Cotter, S., McClelland, A.J., MacEwen, G., 1975, Horizontal transmission of feline leukemia virus in cats. Bibl Haematol, 67-74. 30. Harkin, K.R., Roshto, Y.M., Sullivan, J.T., 2003, Clinical application of a polymerase chain reaction assay for diagnosis of leptospirosis in dogs. J Am Vet Med Assoc 222, 1224-1229. 31. Hayashi, T., Ishida, T., Fujiwara, K., 1982, Glomerulonephritis associated with feline infectious peritonitis. Nippon Juigaku Zasshi 44, 909-916. 32. Heeney, J.L., Evermann, J.F., McKeirnan, A.J., Marker-Kraus, L., Roelke, M.E., Bush, M., Wildt, D.E., Meltzer, D.G., Colly, L., Lukas, J., et al., 1990, Prevalence and implications of feline coronavirus infections of captive and free-ranging cheetahs (Acinonyx jubatus). J Virol 64, 1964-1972. 33. Hodgin, C., Schillhorn van Veen, T.W., Fayer, R., Richter, N., 1984, Leptospirosis and coccidial infection in a guanaco. J Am Vet Med Assoc 185, 1442-1444. 34. Holzworth, J., 1963, Infectious diseases of cats. Cornell Vet 53, 131-143. 35. Hyman, J.A., Johnson, L.K., Tsai, M.M., O'Leary, T.J., 1995, Specificity of polymerase chain reaction identification of Toxoplasma gondii infection in paraffin-embedded animal tissues. J Vet Diagn Invest 7, 275-278. 36. Ishida, T., Washizu, T., Toriyabe, K., Motoyoshi, S., Tomoda, I., Pedersen, N.C., 1989, Feline immunodeficiency virus infection in cats of Japan. J Am Vet Med Assoc 194, 221-225. 37. Jackson, M.L., Haines, D.M., Meric, S.M., Misra, V., 1993, Feline leukemia virus detection by immunohistochemistry and polymerase chain reaction in formalin-fixed, paraffin-embedded tumor tissue from cats with lymphosarcoma. Can J Vet Res 57, 269-276. 38. Jarrett, O., 1991, Overview of feline leukemia virus research. J Am Vet Med Assoc 199, 1279-1281. 39. Jarrett, W.F., Crawford, E.M., Martin, W.B., Davie, F., 1964, A Virus-Like Particle Associated With Leukemia (Lymphosarcoma). Nature 202, 567-569. 40. Kennedy, M., Citino, S., McNabb, A.H., Moffatt, A.S., Gertz, K., Kania, S., 2002, Detection of feline coronavirus in captive Felidae in the USA. J Vet Diagn Invest 14, 520-522. 41. Kidney, B.A., Ellis, J.A., Haines, D.M., Jackson, M.L., 2000, Evaluation of formalin-fixed paraffin-embedded tissues obtained from vaccine site-associated sarcomas of cats for DNA of feline immunodeficiency virus. Am J Vet Res 61, 1037-1041. 42. Kumar, V., Cotran R. S., Robbins, S. L., 2003, Robbins basic pathology. Saunders, pp.509-538. 43. Lilenbaum, W., Monteiro, R.V., Albuquerque, C.E., Ristow, P., Fraguas, S., Cardoso, V.S., Fedullo, L.P., 2004, Leptospiral antibodies in wild felines from Rio de Janeiro Zoo, Brazil. Vet J 168, 191-193. 44. Lilenbaum, W., Monteiro, R.V., Ristow, P., Fraguas, S., Cardoso, V.S., Fedullo, L.P., 2002, Leptospirosis antibodies in mammals from Rio de Janeiro Zoo, Brazil. Res Vet Sci 73, 319-321. 45. Lin, J.A., Cheng, M.C., Inoshima, Y., Tomonaga, K., Miyazawa, T., Tohya, Y., Toh, K., Lu, Y.S., Mikami, T., 1995, Seroepidemiological survey of feline retrovirus infections in cats in Taiwan in 1993 and 1994. J Vet Med Sci 57, 161-163. 46. Lucke, V.M., 1968, Renal disease in the domestic cat. J Pathol Bacteriol 95, 67-91. 47. Lucke, V.M., 1969, Diseases of the urinary system of the cat. Vet Rec 84, 325-329. 48. Lucke, V.M., 1978, Renal disease in the cat. Vet Rec 102, 301-303. 49. Lutz, H., Hofmann-Lehmann, R., Fehr, D., Leutenegger, C., Hartmann, M., Ossent, P., Grob, M., Elgizoli, M., Weilenmann, P., 1996, [Liberation into the wild of wild felines--danger of the release of virus infections]. Schweiz Arch Tierheilkd 138, 579-585. 50. Lutz, H., Isenbugel, E., Lehmann, R., Sabapara, R.H., Wolfensberger, C., 1992, Retrovirus infections in non-domestic felids: serological studies and attempts to isolate a lentivirus. Vet Immunol Immunopathol 35, 215-224. 51. Marker, L., Munson, L., Basson, P.A., Quackenbush, S., 2003, Multicentric T-cell lymphoma associated with feline leukemia virus infection in a captive namibian cheetah (Acinonyx jubatus). J Wildl Dis 39, 690-695. 52. Mason, R.W., King, S.J., McLachlan, N.M., 1972, Suspected leptospirosis in two cats. Aust Vet J 48, 622-623. 53. McCullagh, K.G., Bishop, K.A., Lucke, V.M., Kelly, D.F., 1983, Experimental pyelonephritis in the cat: 3. Collagen alterations in renal fibrosis. J Comp Pathol 93, 9-25. 54. McPherson, M. J., Quirke, P., Taylor, G. R, 1991, PCR a practical approach. IRL PRESS. 55. Meric, S.M., 1984, Suspected feline leukemia virus infection and pancytopenia in a western cougar. J Am Vet Med Assoc 185, 1390-1391. 56. Michna, S.W., 1970, Leptospirosis. Vet Rec 86, 484-496. 57. Modric, Z., Huber, D., 1993, Serologic survey for leptospirae in European brown bears (Ursus arctos) in Croatia. J Wildl Dis 29, 608-611. 58. Nangaku, M., 2004, Mechanisms of tubulointerstitial injury in the kidney: final common pathways to end-stage renal failure. Intern Med 43, 9-17. 59. Neiffer, D.L., Klein, E.C., Wallace-Switalski, C., 2001, Leptospira infection in two black rhinoceroses (Diceros bicornis michaeli). J Zoo Wildl Med 32, 476-486. 60. Paltrinieri, S., Cammarata Parodi, M., Cammarata, G., Mambretti, M., 1998, Type IV hypersensitivity in the pathogenesis of FIPV-induced lesions. Zentralbl Veterinarmed B 45, 151-159. 61. Pearks Wilkerson, A.J., Teeling, E.C., Troyer, J.L., Bar-Gal, G.K., Roelke, M., Marker, L., Pecon-Slattery, J., O'Brien, S.J., 2004, Coronavirus outbreak in cheetahs: lessons for SARS. Curr Biol 14, R227-228. 62. Pedersen, N.C., Black, J.W., Boyle, J.F., Evermann, J.F., McKeirnan, A.J., Ott, R.L., 1984, Pathogenic differences between various feline coronavirus isolates. Adv Exp Med Biol 173, 365-380. 63. Pedersen, N.C., Ward, J., Mengeling, W.L., 1978, Antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species. Arch Virol 58, 45-53. 64. Pedersen, N.C., Yamamoto, J.K., Ishida, T., Hansen, H., 1989, Feline immunodeficiency virus infection. Vet Immunol Immunopathol 21, 111-129. 65. Poli, A., Abramo, F., Cavicchio, P., Bandecchi, P., Ghelardi, E., Pistello, M., 1995, Lentivirus infection in an African lion: a clinical, pathologic and virologic study. J Wildl Dis 31, 70-74. 66. Reinacher, M., Frese, K., 1991, [Glomerulonephritis in dogs and cats]. Tierarztl Prax 19, 175-180. 67. Riemann, H.P., Fowler, M.E., Schulz, T., Lock, A., Thilsted, J., Pulley, L.T., Hendrickson, R.V., Henness, A.M., Franti, C.E., Behymer, D.E., 1974, Toxoplasmosis in pallas cats. J Wildl Dis 10, 471-477. 68. Roelke, M.E., Forrester, D.J., Jacobson, E.R., Kollias, G.V., Scott, F.W., Barr, M.C., Evermann, J.F., Pirtle, E.C., 1993, Seroprevalence of infectious disease agents in free-ranging Florida panthers (Felis concolor coryi). J Wildl Dis 29, 36-49. 69. Saif, L.J., 1993, Coronavirus immunogens. Vet Microbiol 37, 285-297. 70. Schoeman, T., Lobetti, R.G., Jacobson, L.S., Penzhorn, B.L., 2001, Feline babesiosis: signalment, clinical pathology and concurrent infections. J S Afr Vet Assoc 72, 4-11. 71. Strickland, K.N., 1998, Canine and feline caval syndrome. Clin Tech Small Anim Pract 13, 88-95. 72. Treml, F., Nesnalova, E., 1993, [Occurrence of Leptospira antibodies in the blood of game animals]. Vet Med (Praha) 38, 123-127. 73. Tuch, K., Witte, K.H., Wuller, H., 1974, [Diagnosis of feline infections peritonitis (FIP) in domestic cats and leopards in Germany]. Zentralbl Veterinarmed B 21, 426-441. 74. Ward, J.M., Munn, R.J., Gribble, D.H., Dungworth, D.L., 1968, An observation of feline infectious peritonitis. Vet Rec 83, 416-417. 75. Ward, M.P., Guptill, L.F., Wu, C.C., 2004, Evaluation of environmental risk factors for leptospirosis in dogs: 36 cases (1997-2002). J Am Vet Med Assoc 225, 72-77. 76. Watson, A.D., 1994, Leptospirosis in cats and dogs. Aust Vet J 71, 59-60. 77. Werner, L.L., Gorman, N.T., 1984, Immune-mediated disorders of cats. Vet Clin North Am Small Anim Pract 14, 1039-1064. 78. Whyte, P.B., Ratcliff, R.M., Cargill, C., Dobson, K.J., 1982, Protection of pregnant swine by vaccination against Leptospira infection. Aust Vet J 59, 41-45. 79. Yang, C.W., Wu, M.S., Pan, M.J., Hong, J.J., Yu, C.C., Vandewalle, A., Huang, C.C., 2000, Leptospira outer membrane protein activates NF-kappaB and downstream genes expressed in medullary thick ascending limb cells. J Am Soc Nephrol 11, 2017-2026. 80. Yang, C.W., Wu, M.S., Pan, M.J., Hsieh, W.J., Vandewalle, A., Huang, C.C., 2002, The Leptospira outer membrane protein LipL32 induces tubulointerstitial nephritis-mediated gene expression in mouse proximal tubule cells. J Am Soc Nephrol 13, 2037-2045. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35396 | - |
| dc.description.abstract | 於歷年執行動物園野生動物疾病監控調查研究計畫中，針對死亡動物進行病理學檢查，發現貓科動物多具有慢性腎炎（Chronic nephritis）之病理變化。慢性腎炎為一常見形態學診斷，肇因於絲球體腎炎（Glomerulonephritis）、間質性腎炎（Interstitial nephritis）及腎盂腎炎（Pyelonephritis）等各種不同腎臟疾病之末期病變。可導致貓腎臟病變之非感染性因子包括先天缺陷、腎動脈血液供應不良、腎毒性化學物質、重金屬、高血壓及食因性因子等。感染性因子則包括貓傳染性腹膜炎病毒（Feline infectious peritonitis virus, FIPV）、貓白血病病毒（Feline leukemia virus, FeLV）、貓免疫缺陷病毒（Feline immunodeficiency virus, FIV）、鉤端螺旋體（Leptospira spp.）、大腸桿菌（Escherichia coli）、Proteus spp.、Pseudomonas spp.、Klebsiella spp.、鏈球菌（Streptococcus spp.）、Bartonella spp.、弓蟲（Toxoplasma gondii）、犬新胞蟲（Neospora caninum）、心絲蟲（Dirofilaria immitis）等。FIP為貓科動物重要傳染性疾病，易感染所有貓科動物，尤其是獵豹。動物園貓科動物感染FeLV及FIV之病例相當罕見，FeLV雖曾被記載發生於美洲山獅、獵豹與雲豹，而感染FIV亦曾發表於非洲獅、老虎及花豹，但是，目前FeLV與FIV感染症在動物園貓科動物的重要性仍不清楚。至於可引起間質性腎炎之鉤端螺旋體，於1978年曾有兩名動物園管理員經由飼養的熊感染發病，而成為近年來再度受到重視的重現性重要人畜共通傳染病。
本研究利用組織化學染色、免疫組織化學染色、聚合酶鏈鎖反應等方法，回溯性探討動物園內死亡貓科動物引發慢性腎炎之可能病因。收集自1989年至2004年台大獸醫學系及台灣動物科技研究所於北、中、南部各公私立動物園及動物展示場剖檢之貓科動物腎臟組織蠟塊，重新製作組織切片，依據H&E染色進行組織病理變化形態學分析；選擇具有腎炎病變之檢體，利用免疫組織化學染色（Immunohistochemistry）、免疫螢光抗體染色法 (Immunofluorescence)及聚合酶鏈鎖反應 (PCR)，分別偵測腎臟組織內是否有鉤端螺旋體、貓傳染性腹膜炎病毒、貓白血病病毒及貓免疫缺陷病毒等病原的存在。 由歷年收集之177例動物園貓科動物石蠟包埋腎臟檢體，經重新製作組織切片，進行組織病理學分析，結果發現65.5%（116/177）具有腎炎之病理變化，其中以間質性腎炎（Interstitial nephritis）發生頻次最高，佔97.4%（113/116）。利用Leptospira shermani外膜蛋白多源抗體進行免疫組織化學染色，結果發現其陽性率為24.8%（28/113）。另外，利用TGEV棘蛋白多源抗體進行免疫螢光抗體染色，結果於46例具有膜性及/或增殖性絲球體腎炎病例中，發現6例 (6/46, 13%) 於絲球體中出現陽性反應。由177個石蠟包埋腎臟檢體中成功自70個病例萃取出核酸，分別進行鉤端螺旋體、FeLV及FIV之PCR檢測，結果鉤端螺旋體的陽性率為24.3% (17/70)，FeLV的陽性率為2.9% (2/70)，而FIV則完全未發現陽性病例 (0/70)。此結果顯示，鉤端螺旋體可能為國內動物園貓科動物慢性腎炎的重要致病因子，至於FIPV、FeLV及FIV等病毒之感染則所佔比率極低。 | zh_TW |
| dc.description.abstract | During routing health monitoring and disease surveillance for zoo animals, it was revealed that chronic interstitial nephritis was a common pathological finding in zoo felids. Chronic interstitial nephritis may represent the end result of several different renal diseases, including glomerulonephritis, interstitial nephritis, and chronic pyelonephritis. The non-infectious causes of chronic renal diseases in the cat include congenital disorders, decreased blood supply to renal arteries, toxic chemicals, heavy metals, hypertension, and dietary; the infectious causes include feline infectious peritonitis virus (FIPV), feline leukemia virus (FeLV), and feline immunodeficiency virus (FIV), Leptospira spp., Escherichia coli, Proteus spp., Pseudomonas spp., Klebsiella spp., Streptococcus spp., Bartonella spp., Toxoplasma gondii, Neospora caninum, and Dirofilaria immitis etc. All felid species are susceptible to FIPV but the disease has a higher prevalence in certain species such as the cheetah. Infections of FeLV and FIV are only rarely reported in exotic feline species, such as African lions, tigers, and panthers. The clinical significance of FIV and FeLV in zoo felids is still unknown. Leptospirosis is a zoonotic emerging infectious diseases causing interstitial nephritis in man and animals and has been reported in zoo workers acquired from bear cubs. This observation suggests that zoonotic leptospiral infection should be considered as a possible pathogen for zoo felids as well.
One hundred and seventy-seven paraffin embedded renal tissues of the zoo felids collected during 1989 and 2004 were subjected to histopathological examination. Immunohistochemistry (IHC) was used to detect Leptospira spp. and immunofluorsecence antibody test (IFA) was used for feline coronavirus. Polymerase chain reaction (PCR) was used to detect Leptospira spp., FeLV, and FIV. In the 177 cases, 65.5%（116/177）showed evidence of nephritis. Within the 116 cases of nephritis, 97.4%（113/116） were classified as interstitial nephritis. By applying the polyclonal antibody against the outer membrane protein of Leptospira shermani by IHC, a 24.8% (28/113) positive rate was revealed. In addition, the analysis of FIPV antigens in the kidneys of 46 felids with membrano- and/or proliferative glomerulonephritis using IFA by a polyclonal antibody against the spike protein of TGEV revealed that 6 out of 46 (6/46, 13%) cases showing positive signals in the glomeruli. Nucleic acid was successfully extracted from the tissue block of 70 out of 177 cases. Seventeen out of the 70 cases (17/70, 24.3%) were positive for Leptospira, 2 out of the 70 cases (2/70, 2.9%) were positive for FeLV, but none of the cases (0/70) was positive for FIV by PCR. The results suggest that leptospira should be considered as a possible important pathogen in association with the common observed chronic nephritis in zoo felids. The role of FIPV, FeLV, and FIV in chronic nephritis of zoo felids is minor. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:51:01Z (GMT). No. of bitstreams: 1 ntu-94-R92629022-1.pdf: 2545703 bytes, checksum: 8647cb7121d1572c7596fbc68ee2bc19 (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 目錄 I
表次 IV 圖次 V 中文摘要 VII 英文摘要 IX 第一章 緒言……………………………………………………………………….. 1 第二章 文獻探討………………………………………………………………….. 3 第一節 潛在可能引發動物園貓科動物腎臟病變之病因………………… 3 第二節 腎臟病變之致病機制…………………………………………….. 4 2.1 腎臟的功能…………………………………………………... 4 2.2 腎臟病變之致病機制………………………………….......... 5 2.3 鉤端螺旋體病的致病機制…………………………………... 9 2.4 病毒性疾病引發腎臟病變之致病機制……………………... 10 第三節 鉤端螺旋體在動物間的致病性………………………………….. 10 3.1 鉤端螺旋體的發現…………………………………………... 11 3.2 鉤端螺旋體之分類與特性…………………………………... 11 3.3 鉤端螺旋體在動物間扮演的致病角色……………………... 13 3.4 鉤端螺旋體與動物園圈飼及野生動物之關係……………... 15 第四節 貓傳染性腹膜炎病毒與貓科動物之關係……………………….. 16 4.1 FIP的歷史背景與概況………………………………………. 16 4.2 FIP的病毒特性………………………………………………. 17 4.3 FIPV與動物園貓科動物之關係…………………………….. 20 第五節 貓白血病病毒與動物園貓科動物之關係……………………….. 20 5.1 FeLV的病毒特性……………………………………………. 20 5.2 FeLV與動物園圈飼及野外貓科動物之關係……………….. 21 第六節 貓免疫缺陷病毒與貓科動物之關係…………………………….. 22 6.1 FIV的病毒特性………………………………………………. 22 6.2 FIV與動物園圈飼及野外貓科動物之關係…………………. 23 第三章 材料與方法……………………………………………………………….. 24 第一節 實驗設計………………………………………………………….. 24 第二節 實驗材料………………………………………………………….. 25 2.1 動物園貓科動物腎臟組織蠟塊樣本之收集……………….. 25 2.2 組織化學染色……………………………………………….. 25 2.3 免疫染色……………………………………………………... 26 2.3.1 應用於鉤端螺旋體之免疫組織化學染色檢測試劑…………………………………………………….. 26 2.3.2 應用於貓傳染性腹膜炎病毒之免疫螢光染色檢測試劑…………………………………………………….. 26 2.3.2.1 直接免疫螢光抗體染色法……………… 26 2.3.2.2 間接免疫螢光抗體染色法……………... 27 2.4聚合酶鏈鎖反應……………………………………………… 27 2.4.1 應用於萃取蠟塊組織中DNA之試劑………………. 27 2.4.2 應用於PCR反應與電泳之試劑……………………. 27 第三節 實驗方法………………………………………………………….. 30 3.1 蠟塊組織之DNA萃取………………………………………. 30 3.2 組織化學染色法與腎臟病變之型別分析…………………… 30 3.2.1 組織化學染色法-蘇木紫與伊紅染色法……………... 30 3.2.2腎臟組織病變之型別分析…………………………….. 31 3.3 免疫染色……………………………………………………… 31 3.3.1 應用免疫組織化學染色法於鉤端螺旋體之檢測………………………………………………………. 31 3.3.2 應用免疫螢光染色法於貓傳染性腹膜炎病原之檢測. 32 3.3.2.1 直接免疫螢光抗體染色法………………. 32 3.3.2.2 間接免疫螢光抗體染色法………………. 33 3.4 聚合酶鏈鎖反應……………………………………………… 33 3.4.1 16s DNA之檢測………………………………………. 33 3.4.2 鉤端螺旋體之檢測……………………………………. 34 3.4.3 貓白血病病毒之檢測…………………………………. 34 3.4.4 貓免疫缺陷病毒之檢測………………………………. 35 3.4.5 洋菜膠電泳……………………………………………. 35 第四章 結果……………………………………………………………………….. 37 第一節 動物園貓科動物背景資料、腎臟病變之型別與年齡分析………………………………………………………………… 37 1.1 背景資料……………………………………………………… 37 1.2 年齡分析……………………………………………………… 39 1.3 貓科動物解剖例之腎炎年發生率…………………………… 41 1.3.1 五月齡以下貓科動物腎臟組織病理學變化與發生頻次…………………………………………………….. 44 1.3.2 五月齡至三歲………………………………………… 44 1.3.3 三歲至十歲…………………………………………… 44 1.3.4 十歲以上……………………………………………… 45 1.3.5 年齡不詳……………………………………………… 45 第二節 動物園貓科動物鉤端螺旋體病之陽性率………………………… 48 2.1 應用免疫組織化學染色檢測腎臟組織中Leptospira的抗原及其檢出陽性率…………………………………………….. 48 2.2 應用聚合酶鏈鎖反應檢測腎臟組織中Leptospira核酸及其檢出陽性率………………………………………………….. 51 2.3 應用聚合酶鏈鎖反應與免疫組織化學染色檢測Leptospira陽性病例腎臟之病理學特徵……………………………….. 53 第三節 應用免疫組織化學染色檢測腎臟組織中貓傳染性腹膜炎 (Feline infectious peritonitis) 病毒抗原及其檢出陽性率……….................................................................................. 57 3.1 直接免疫螢光抗體染色法……………………………… 57 3.2 間接免疫螢光抗體染色法……………………………… 57 第四節 應用聚合酶鏈鎖反應檢測腎臟組織中貓白血病病毒 (Feline leukemia virus) 核酸及其檢出陽性率………………………… 61 第五節 應用聚合酶鏈鎖反應檢測腎臟組織中貓免疫缺陷病毒 (Feline immunodeficiency virus) 核酸及其檢出陽性率…………….. 63 第五章 討論………………………………………………………………………… 64 參考文獻…………………………………………………………………………….. 74 附錄 81 | |
| dc.language.iso | zh-TW | |
| dc.title | 動物園貓科動物慢性腎炎之微生物性病因探討 | zh_TW |
| dc.title | Microbial etiological survey of felid chronic nephritis in zoo | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 鄭謙仁(Chian-Ren Jeng) | |
| dc.contributor.oralexamcommittee | 鄭益謙 | |
| dc.subject.keyword | 貓科動物,慢性腎炎,鉤端螺旋體,貓傳染性腹膜炎, | zh_TW |
| dc.subject.keyword | Felid,Chronic Nephritis,Leptospira,Feline Infectious Peritonitis, | en |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2005-07-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 目前未授權公開取用 | 2.49 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。