請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35244
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳世銘 | |
dc.contributor.author | Jin-Nan Chen | en |
dc.contributor.author | 陳金男 | zh_TW |
dc.date.accessioned | 2021-06-13T06:45:17Z | - |
dc.date.available | 2005-08-01 | |
dc.date.copyright | 2005-08-01 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-29 | |
dc.identifier.citation | 方煒、饒瑞佶。2004。高亮度發光二極體在生物產業之應用。中華農學會報5(5):432-446。
田秉才、陳世銘、馮丁樹。1989。檸檬顏色選別裝置之研製。農業工程學報35(4):73-82。 高式寧。2003。不同給光方式之電腦模擬及其對彩色海芋組培苗生長之影響。碩士論文。台北:國立臺灣大學生物產業機電工程學研究所。 康志忠。2000。植物組織培養瓶噴射式自動洗瓶機之研發。碩士論文。台中:國立中興大學農業機械工程學研究所。 張元聰、林棟樑 、王仕賢 、張錦興。2000。嘉南地區星辰花產業簡介。台南區農業專訊31:13-17。網址: http://www.tndais.gov.tw/Magazine/ mag31-5.htm。 許欣正。2002。光質對組培苗生長之研究。碩士論文。台中:國立中興大學農業機械工程學研究所。 陳世銘、馮丁樹、洪慎德、吳春杰、呂昆忠、田秉才、張文宏。1996。柑橘類水果光電選別機之研製。農業機械學刊5(4):35-46。 陳加忠。2000。組織培養苗生產自動化之技術開發。組織培養苗量產工程推廣手冊,1-7。 陳金男、陳世銘、楊宜璋、李進發。2003a。以發光二極體作為組織培養光源之光環境模擬。出自”九十二年農機與生機論文發表會論文摘要集”,125-126。台北:中華農業機械學會。 陳金男、陳世銘、楊宜璋、李進發。2004。以發光二極體作為組培光源之製作與電腦圖控系統之建立。2004生機與農機論文發表會論文摘要集。233-234。台北:台灣生物機電學會。 陳金男。2003。以發光二極體作為瓶苗栽培光源之光環境模擬與控制。學士論文。台北。國立台灣大學生物產業基機電工程學系。 陳南叡、陳世銘、楊蕙綺。2003b。組織培養瓶內光及溫度分佈之探討。出自”九十二年農機與生機論文發表會論文摘要集”,123-124。台北:中華農業機械學會。 馮丁樹。1998。種苗生產自動化技術專輯第三期第98011號。台北。 黃慶文。2002。植物組織培養瓶換氣率與透光量之研究。碩士論文。台中:國立中興大學農業機械工程學研究所。 褚昱均。2004。出瓶光度與培植時期對數種天南星科植物組培苗出瓶後生長之影響。碩士論文。台北。國立台灣大學園藝學研究所。 賴建洲、饒瑞佶、方煒、張森富。2004。交流供電的紅光發光二極體應用於彩色海芋組織培養苗量產栽培之可行性探討。中華農學會報5(5):493-502。 謝廣文、陳世銘。1988。番茄顏色之光電選別室設計。農業工程學報34(4):39-46。 饒瑞佶、方煒、李登華。2001。超高亮度發光二極體做為組培苗栽培人工光源之燈具製作與應用。中國園藝47(3):301-312。 饒瑞佶、方煒。2000。組合式紅、藍光發光二極體燈具之給光環境模擬。農業機械學刊9(3):51-63。 饒瑞佶。2003。發光二極體應用於蝴蝶蘭、彩色海芋與馬鈴薯組織培養之研究。博士論文。台北:國立臺灣大學生物產業機電工程學研究所。 Aksenova, N. P., T. N. Konstantinova, L. I. Sergeeva, I. Machackova, and S. A. Golyanovskaya. 1994. Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones. J. Plant Growth Reaul. 13:143-146. Appelgren, M. 1991. Effects of light quality on stem elongation of Pelargonium in vitro. Scientia Horticulture. 45:345-351. Attridge, T. H. 1990. Light and plant responses. 1st ed. New York : Routledge, Chapman and Hall, Inc. Bukhov, N. G., I. S. Drozdova, and V. V. Bondar. 1995. Light response curves of photosynthesis in leaves of sun-type and shade-type plants grown in blue or red light. Journal of photochemistry and photobiology B:Biology 30:39-41. Bula, R. J., R. C. Morrow, T. W. Tibbitts, and D. J. Barta. 1991. Light-emitting diodes as a radiation source for plants. HortScience 26(2):203-205. Carvalho, L., and S. Amancio. 2002. Effects of ex vitro conditions on growth and acquisition of autotrophic behavior during the acclimatization of chestnut regenerated in vitro. Scientia Horticulturae 95:151-164. Chen, S., J. N. Chen, H. C. Yang, I. C. Yang, C. T. Chen and C. F. Lee. 2004a. Simulation and control of LED lighting system for plantlets in vitro. In “Proceedings of 8th Asia Pacific Orchid Conference”, 180-196. Taipei: Taiwan Orchid Growers Association. Chen, S., J. N. Chen, H. C. Yang. I. C. Yang, C. T. Chen, C. F. Lee. 2004b. Light environment simulation and control using LED as a light source for plantlets in vitro. In “Proceedings of the Second International Symposium on Machinery and Mechatronics for Agriculture and Bio-systems Engineering”, S3-1 ~ 4. Kobe, Japan: Kobe University Debergh, P. C. 1991. Acclimatization techniques of plants from in vitro. Acta Horticulturae 289:291-300. Economou, A. S., and P. E. Read. 1987. Light treatments to improve efficiency of in vitro propagation system. HortScience 22(5):751-754. Gautier, H., C. Varlet-Grancher, and N. Baudry. 1997. Effects of blue light on the vertical colonization of space by White Clover and their consequences for dry matter distribution. Annals of Botany 80:665-671. Genoud, C., A. Coudret, C. Amalric, and H. Sallanon. 1999. Effects of micropropagation conditions of rose shootlets on chlorophyll fluorescence. Photosynthetica 36:243-251. Ghashghaie, J., F. Brenckmann, and B. Saugier. 1992. Water relations and growth of rose plants cultured in vitro under various relative humidities. Plant Cell, Tissue, Organ Culture 30:51-57. Goins, G. D., N. C. Yorio, M. M. Sanwo, and C. S. Brown. 1997. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes(LEDs) with and without supplemental blue lighting. J. Exp. Bot. 48:1407-1413. Hoenecke, M. E., R. J. Bula, and T, W. Tibbitts. 1992. Importance of “Blue” photon levels for Lettuce seedlings grown under red-light-emitting diodes. HortScience 27(5):427-430. Holmes, M. G., and E. Wagner. 1980. A re-evaluation of phytochrome involvement in time measurement in plants. J. Theor. Biol 83:255-265. Jao, R. C., and W. Fang. 2003. An adjustable light source for photo-phyto related research and young plant production. Applied Engineering In Agriculture. 19(5):601-608. Jao, R. C., and W. Fang. 2004a. Effects of frequency and duty ratio on the growth of potato plantlets in vitro using light-emitting diodes. HortScience. 39(2):375-379. Jao, R. C., and W. Fang. 2004b. Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods. HortScience. 39(2):380-382. Jeong, B. R. 1996. Stem elongation and growth of Mentha rotundifolia in vitro as influenced by photoperiod, photosynthetic photon flux, and difference between say and night temperatures. Acta Hort. 440:539-543. Kadlecek, P., I. Ticha, D. Haisel, V. Capkova, and C. Schafer. 2001. Importance of in vitro pretreatment for ex vitro acclimatization and growth. Plant Science 161:695-701. Karim, A., H. Fukamachi, and T. Hidaka. 2003. Photosynthetic performance of Vigna radiata L. leaves developed at different temperature and irradiance levels. Plant Science 164:451-458. Kozai, T., H. Oki, and K. Fujiwara. 1990. Photosynthetic characteristics of Cymbidium plantlet in vitro. Plant Cell, Tissue and Organ Culture 22:205-211. Kozai, T., K. Watanabe, and B. R. 1995. Stem elongation and growth of Solanum tuberosum L. in vitro in response to photosynthetic photon flux, photoperiod and different in photoperiod and dark period temperatures, Scientia Horticulturae 64:1-9. Lian, M. L., H. N. Murthy, and K. Y. Paek. 2002a. Culture method and photosynthetic photon flux affect photosynthesis, growth and survival of Limonium “Misty Blue” in vitro. Scientia Horticulturae 95:239-249. Lian, M. L., H. N. Murthy, and K. Y. Paek. 2002b. Effects of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid “Pesaro”. Scientia Horticulturae 94:365-370. Maas, F. M., L. B. Hofman-Eijer, and K. Hulsteijn. 1995. Flower morphogenesis in Rosa “Mercedes” as studied by cryo-scanning electron and light microscopy. Effects of light and shoot position on a branch. Annals of Botany 75:199-205. Marek, M. V., M. Sprtova, and P. D. Angelis. 2001. Spatial distribution of photosynthetic response to long-term influence of elevated CO2 in a mediterranean macchia mini-ecosystem. Plant Science 160:1125-1136. McCain, D. C., J. Croxdale, and J. I. Markley. 1988. Water is allocated differently in chloroplasts in sun and shade leaves. Plant Physiol. 86:16-18. Meziane, D., and B. Shipley. 2001. Direct and indirect relationships between specific leaf area, leaf nitrogen and leaf gas exchange. Effects if irradiance and nutrient supply. Annals of Botany 88:915-927. Miyashita, Y., Y. Kitaya, and T. Kozai. 1995. Effects of red and far-red light on the growth and morphology plantlets in vitro: using light-emitting diode as a light source for micropropagation. Acta Horticulturae 393: 189-194. Nowak, J., S. Sroka, and B. Matysiak. 2002. Effects of light level, CO2 enrichment, and concentration of nutrient solution on growth, leaf nutrient content, and chlorophyll fluorescence of Boston fern microcuttings. J. Plant Nutr. 25:2161-2171. Okamoto, K., T. Yanagi, and S. Kondo. 1997. Growth and morphogenesis of lettuce seedlings raised under different combinations of red and blue lights. Acta Horticuturae. 435:149-157. Okamoto, K., T. Yanagi, and S. Takita. 1996. Development of plant growth apparatus using blue and red LED as artificial light source. Acta Horticulturae 440:111-116. One, E., J. L. Cuello, and K. A. Jordan. 1997. Evaluation of high intensity light-emitting diodes as light source for plant growth. ASAE N0. 974028. Piqueras, A., J. M. Van Huylenbroeck, B. H. Han, and P. C. Debergh. 1998. Carbohydrate partitioning and metabolism during acclimatization of micropropagated Calathea. Plant Growth Regulation 26:25-31. Sabo, A., T. Krekling, and M. Appelgren. 1995. Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell, Tissue and Organ Culture 41:177-185. Schuerger, A. C., C. S. Brown, and E. C. Stryjewski. 1997. Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Annals of Botany. 79:273-282. Sedra, A. S., and Smith K. C. 1998. Microelectronic Circuits. 4th, 137-155. New York. Oxford University Press, Inc. Shillo, R., M. Ding, D. Pasternak, and M. Zaccai. 2002. Cultivation of cut flower and bulb species with saline water. Scientia Horticulturae 92:41-54. Sinclair, T. R., J. D. Ray, L, M. Premazzi, and P. Mislevy. 2004. Photosynthetic photon flux density influences grass responses to extended photoperiod. Environmental and Experimental Botany 51:69-74. Takita, S., K. Okamoto, and T. Yanagi. 1996. Computer simulation of PPF distribution under blue and red LED light source for plant growth. Acta Horticulturae 440:286-291. Tanaka, M., T. Takamura, H. watanabe, M. Endo, and T. Yanagi. 1998. In vitro growth of Cymbidium plantlets cultured under superbright red and blue light-emitting diodes (LEDs). Journal of horticultural science & biotechnology. 73(1):39-44. Tani, T., H. Kudoh, and N. Kachi. 2001. Responses of photosynthesis and biomass allocation of understorey herb, Pteridophyllum racemosum, to gradual increases in irradiance. Annals of Botany 88:393-402. Ting, K. C., and G. A. Giacomelli. 1987. Availability of solar photosynthetically active radiation. ASAE 30(5):1453-1457. Tripathy, B. C., and C. S. Brown. 1995. Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiology. 107:407-411. Van Huylenbroeck, J. M., A. Piqueras, and P.C. Debergh. 1998. Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micro propagated plants. Plant Science 134:21-30. Van Huylenbroeck, J. M., and J. D. Riek. 1995. Sugar and starch metabolism during ex vitro rooting and acclimatization of micropropagated Spathiphyllum ’Petite’ plantlets. Plant Science 111:19-25. Van Huylenbroeck, J. M., and P. C. Debergh. 1996. Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets. Physiologia Plantarum 96:298-304. Warner, R. M., and J. E. Erwin. 2003. Effect of photoperiod and daily light integral on flowering of five hibiscus sp. Scientia Horticulturae 97:341-351. Yanagi, T., K. Okamoto, and S. Takita. 1996. Effects of blue, red, and blue/red lights of two different PPF levels on growth and morphogenesis of lettuce plants. Acta Horticulturae 440: 117-122. Yang, H. C., S. Chen, M. H. Jean and C. T. Chen. 2002. The effects of light quality and intensity on in vitro growth of phalaenopsis plantlets. In “Proceedings of International Symposium on Automation and Mechatronics of Agricultural and Bioproduction Systems”, 623-628. Chiayi, Taiwan : National Chiayi University. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35244 | - |
dc.description.abstract | 發光二極體( Light-Emitting Diode, LED )近年來逐漸被廣泛的運用,其中之一為提供植物組織培養之人工栽培光源,主要因為其具有較高的光電轉換效率、熱產生較一般日光燈管低與波長特定等優點。本研究使用紅、藍光兩種 LED 開發一套應用於組織培養上之光環境控制系統,並利用 LabVIEW 軟體自行開發光源模擬與控制程式進行操作。陣列式紅、藍光 LED 模組可設定多樣性之顯示來改變光質特性,且透過獨自改變紅、藍光 LED 之光強度可控制整體光源之光質與光度特性,最後以光週期控制程式進行栽培期間內時間之監控,達到具有彈性調控光源之光質、光度與光週等功能。於光源中心正下方 10 cm 處,純紅光與純藍光 LED 模組可提供約 150 μmol m-2 s-1 之光強度,而紅、藍光 LED 混合模組則提供約 80 μmol m-2 s-1 紅光與約 50 μmol m-2 s-1藍光之光強度。
利用上述之光環境控制系統製造出純紅光、純藍光、紅藍比1:2與2:1等四種光質特性之光環境,控制總光度各約為 100μmol m-2 s-1 (離樣本之高度為 5 cm ),於星辰花組培苗栽培試驗及出瓶後馴化,並以傳統日光燈管光源栽培作為差異比較。結果顯示:光質主要影響葉片之型態與功能,純藍光下生長之葉片較細長、葉色較青與葉綠素含量較低等,隨紅光比例的增加,所生長之葉片愈接近於日光燈管下之葉片;五種處理作物均能生長與分化,於乾物累積上無顯著差異。出瓶馴化階段各處理樣本在日光燈管光源下各差異均能恢復至正常範圍,顯示LED人工光源可應用於組織培養上並可探討光質對植物葉片型態之影響。 | zh_TW |
dc.description.abstract | Light-Emitting Diodes (LED) are widely used every walk of life recently such as artificial light for plants. There are many advantages like specified wavelength, higher electric-light transformation performance and lower heat generation. The study is aimed to develop a light environment control system (LECS) using red and blue light LED’s as a light source. A light simulation and control program was developed to integrate and control the LECS system using LabVIEW software. Using the program, the LED-array can be arranged at random to define different aspects of light quality. Individual setting the light intensity of red and blue LED’s can also change the light quality and intensity simultaneously. The program can monitor the control photoperiods when applying to plant cultivation. Therefore, light quality, light intensity and photoperiod can be controlled flexibly when LECS system is used. The red LED module and blue LED module can individually provide a light intensity of 150 mmol m-2 s-1, and the red/blue LED mixed module can provide about 80 mmol m-2 s-1 for red light, and about 50 mmol m-2 s-1 for blue light at 10 cm height.
Using the developed LECS system, four settings of light quality (pure red light, pure blue light, R/B ratio = 1:2 and R/B ratio = 2:1) were applied to tissue culture experiments of Limonium. The results show that: leaves developed under pure blue light become thiner, whiter and lower in chlorophyll content. When the proportion of red light is increasing, leaves are more close to those developed under tube fluorescent light. There is no significant difference in wet and dry weight among all the treatments. All the samples can be recovered to a normal growth when tube fluorescent light is provided during ex vitro acclimatization. The light environment control system developed in this study has potential applications to tissue culture production and the study of light quality effects on morphogenesis and physiology of plants. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T06:45:17Z (GMT). No. of bitstreams: 1 ntu-94-R92631004-1.pdf: 5105503 bytes, checksum: 4a731da50c5b0104aba18b3d59d85db9 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 誌 謝 i
摘 要 ii Abstract iii 目 錄 iv 圖目錄 viii 表目錄 xii 第一章 前 言 1 1-1 前言 1 1-2 研究目的 3 第二章 文獻探討 4 2-1 光與植物 4 2-2 人工光源與LED光源 10 2-2-1 人工光源 10 2-2-2 LED光源 10 2-3 植物組織培養 13 2-4 LED光源在組培上之應用 20 2-5 星辰花 ( Limonium ) 24 第三章 材料與方法 26 3-1 LED光控系統 26 3-1-1 光控系統硬體之設計 27 3-1-1-1 LED模組 28 3-1-1-2 光源驅動電路 33 3-1-1-3 光源控制電路 36 3-1-1-4 訊號接收與儲存電路 39 3-1-1-5 硬體整合設計 42 3-1-2 光控系統軟體之設計 44 3-2 系統開發與測試 48 3-2-1 硬體材料 48 3-2-2 系統製作與開發 51 3-2-3 系統性能測試 52 3-2-3-1 系統光質與光度控制之測試 52 3-2-3-2 驅動電路性能之測試 53 3-2-3-3 LED光質模組 53 3-3 星辰花組培苗之實驗材料與方法 55 3-3-1 實驗材料 55 3-3-2 實驗設備 56 3-3-2-1 光控系統與生長室 56 3-3-2-2 量測設備 57 3-3-3 實驗方法 63 3-3-3-1 瓶苗栽培實驗方法 63 3-3-3-2 出瓶馴化實驗方法 65 第四章 結果與討論 67 4-1 光控系統之實體 67 4-1-1 光控系統之硬體模組 67 4-1-1-1 LED模組 67 4-1-1-2 光源驅動電路 69 4-1-1-3光源控制電路 69 4-1-1-4訊號接收與儲存電路 70 4-1-1-5硬體整合設計 71 4-1-2 光控系統控制軟體 73 4-1-2-1 硬體設定程式 73 4-1-2-2 光控系統自動控制程式 74 4-1-3 光控系統之整合 75 4-2 光控系統之性能測試結果 76 4-2-1 系統之光質與光度測試結果 76 4-2-2 驅動電路性能之測試 79 4-2-3 LED光質模組 81 4-3 星辰花組培苗之實驗結果與分析 83 4-3-1 瓶苗栽培之實驗結果 83 4-3-2 出瓶馴化實驗結果 90 第五章 結論與建議 98 5-1 結論 98 5-2 建議 101 參考文獻 102 附錄A FPGA晶片功能設計之程式碼 109 | |
dc.language.iso | zh-TW | |
dc.title | LED光環境控制系統之建立與其應用於星辰花組培苗生理影響之探討 | zh_TW |
dc.title | Development of LED Light Environment Control System and its Application to the Study of Physiological Responses in vitro and during ex vitro Acclimatization of Limonium | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 邱奕志,葉德銘,江昭皚,黃裕益 | |
dc.subject.keyword | 發光二極體,光環境控制系統,光質,組織培養,星辰花, | zh_TW |
dc.subject.keyword | LED,Light Environment Control System,Light Quality,Tissue Culture,Limonium, | en |
dc.relation.page | 109 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
顯示於系所單位: | 生物機電工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-94-1.pdf 目前未授權公開取用 | 4.99 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。