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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊恩誠 | |
| dc.contributor.author | Chung-Wei Chiu | en |
| dc.contributor.author | 邱崇瑋 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:36:12Z | - |
| dc.date.available | 2013-07-27 | |
| dc.date.copyright | 2011-07-27 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-25 | |
| dc.identifier.citation | Alagarmalai J, Nestel D. 2009. Identification of host attractants for the Ethiopian fruit fly, Dacus ciliatus Loew. J Chem Ecol 35: 542-551.
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Steiner LF, Lee RKS. 1955. Large-area tests of a male-annihilation method for oriental fruit fly control. J Econ Entomol 48: 311-317. Steiner LF, Mitchell WC, Harris EJ, Kozuma TT, Fujimoto MS. 1965. Oriental fruit fly eradication by male annihilation. J Econ Entomol 58: 961-964. Steiner LF, Hart WG, Harris EJ, Cunningham RT, Ohinata K, Kamakahi DC. 1970. Eradication of the oriental fruit fly from the Mariana Islands by the methods of male annihilation and sterile insect release. J Econ Entomol 63: 131-135. Subchev M, Toshova T, Koshio C, Franke S, Troger A, Twele R, Francke W, Pickett JA, Wadhams LJ, Woodcock CM. 2009. Identification and biological activity of sex pheromone components from females of the plum moth Illiberis rotundata Jordan (Lepidoptera: Zygaenidae: Procridinae). Chemoecology 19: 47-54. Tan KH, Nishida R. 1996. Sex pheromone and mating competition after methyl eugenol consumption in the Bactrocera dorsalis complex. pp. 147-153. In: McPheron BA, Steck GJ (eds). Fruit Fly Pests: a world assessment of their biology and management. St. Lucie Press, Florida. Tan KH, Kirton LG, Serit M. 1986. Age response of Dacus dorsalis (Hendel) to methyl eugenol in a) a wind tunnel and b) traps set in a village, and its implication in population estimation. pp. 425-432. In: Economopoulos AP (eds). Fruit flies: proceedings of the second international symposium. Elsevier science publishers, New York, U.S.A. Wakamura S, Yasui H, Akino T, Yasuda T, Fukaya M, Tanaka S, Maeda T, Arakaki N, Nagayama A, Sadoyama Y, Kishita M, Oyafuso A, Hokama Y, Kobayashi A, Tarora K, Uesato T, Miyagi A, Osuga J. 2009. Identification of (R)-2-butanol as a sex attractant pheromone of the white grub sugarcane pest in the Miyako Islands of Japan. Appl Entomol Zool 44: 231-239. Webb JC, Sharp JL, Chambers DL, McDow JJ, Benner JC. 1976. Analysis and identification of sounds produced by the male Caribbean fruit fly, Anastrepha suspensa. Ann Entomol Soc Am 69: 415-420. Wee SL, Tan KH. 2007. Temporal accumulation of phenylpropanoids in male fruit flies, Bactrocera dorsalis and B. carambolae (Diptera: Tephritidae) following methyl eugenol consumption. Chemoecology 17: 81-85. Wicker-Thomas C. 2007. Pheromonal communication involved in courtship behavior in Diptera. J. Insect Physiol. 53: 1089-1100. Wong TTY, McInnis DO, Nishimoto JI. 1989. Relationship of sexual maturation rate to response of oriental fruit fly strains (Diptera: Tephritidae) to methyl eugenol. J Chem Ecol 15: 1399-1405. Wu WY, Chen YP, Yang EC. 2007. Chromatic cues to trap the oriental fruit fly, Bactrocera dorsalis. J Insect Physiol 53: 509-516. Wyatt TD. 2003. Pheromones and animal behaviour - Communication by small and taste. Cambridge University Press, UK. 391p. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34885 | - |
| dc.description.abstract | 東方果實蠅 (Bactrocera dorsalis (Hendel) ) 是台灣重要的果樹害蟲,對於危害的雌蟲,沒有有效的防治策略,所以研究雌蟲誘引物質顯得相當重要,本研究將分析與鑑定東方果實蠅雄蟲釋放的性費洛蒙。觀察雄蟲的求偶行為,僅在黃昏時產生振翅行為,幫助費洛蒙擴散,以誘引雌蟲。在Y型飛行通道中試驗活雄蟲氣味對雌蟲的誘引效力,有32 ± 1%的雌蟲可被誘引,且與對照組 (1%) 有顯著差異。使用全身萃取法萃取雄蟲釋放之氣味,以氣相層析質譜儀 (GC-MS) 分析,methyl eugenol-fed (ME-fed) 雄蟲萃取液有37種化合物,其中16種為已知化合物。已知化合物中有7種與methyl eugenol (ME) 有密切相關,包括4-vinyl-2-methoxy-phenol (4V2MP)、eugenol、isoeugenol、1-methyoxy-2-(1-methyl-2-propenyl) benzene、methyl isoeugenol、2-allyl-4,5- dimethoxyphenol (DMP) 和coniferyl alcohol (CA),而且這些化合物在non-ME-fed雄蟲萃取液中未被發現。以氣相層析儀-觸角電圖 (GC-EAD) 分析雄蟲萃取液中的活性成分,由結果得知N-(3-methylbutyl) acetamide (NMBA)、4V2MP、Eugenol、ME及DMP能使雌蟲產生電生理反應。除了DMP以外,其餘的活性化合物皆已獲得,且進行雌蟲的誘引效力試驗。在小帳棚內以透明塑膠陷阱進行雌蟲誘引試驗結果中,ME (雌蟲誘引率為45.52 ± 5.21%) 和混合物 (雌蟲誘引率為53.68 ± 6.29%) 的誘引效力較佳,與其餘的活性化合物間有顯著差異。在網室內以黏蟲紙進行誘引試驗結果中,ME (雌蟲誘引率為23.17 ± 1.83%) 和混合物 (雌蟲誘引率為32.17 ± 2.24%) 的誘引效果較佳,除了Eugenol與ME間沒有顯著差異外,ME和混合物與其餘活性化合物間有顯著差異。混合物有最高的誘引比率,而次要化合物單品的誘引比率低,可能對主要化合物有協力作用,但是混合物與ME的誘引效果間沒有顯著差異,可能是混合物中缺少DMP的緣故。活性化合物在田間的誘引效力尚待評估。 | zh_TW |
| dc.description.abstract | Oriental fruit fly (OFF), Bactrocera dorsalis (Hendel), is one of most important pests in Taiwan. However, the female attractant, such as sex pheromone, used for controlling OFF has not been available. In this study, male-released sex pheromone was identified and analyzed. Male performs wing vibration for dispersing sex pheromone at dusk. Y tunnel experiment confirms the behavior that male attracts female by odor cue. The result shows male-released odor attracted 32 ± 1% females, and it differs significantly from control. Basing on this phenomenon, blends were extracted from male flies by whole-body extraction method. The extractions were analyzed by gas chromatography-mass spectrometry (GC-MS). The methyl eugenol-fed (ME-fed) male extraction contains 37 compounds. And 7 compounds, including 4-vinyl-2-methoxy- phenol (4V2MP), eugenol, isoeugenol, 1-methyoxy-2-(1-methyl-2-propenyl) benzene, methyl isoeugenol, 2-allyl-4,5-dimethoxyphenol (DMP), and coniferyl alcohol (CA) are ME-derived among those identifiable compounds. Further analysis by gas chromatography-electroantennographic detection (GC-EAD) is adopted for screening out the active compounds. Five active compounds, including N-(3-methylbutyl) acetamide (NMBA), 4V2MP, Eugenol, ME, and DMP, have been identified, and were further tested by behavioral experiment to determine the attractiveness to the females excluding DMP. In the insect rearing tent, ME (45.52 ± 5.21%) and blend (53.68 ± 6.29%) attracted significantly more females than other active compounds. In the greenhouse, ME (23.17 ± 1.83%) and blend (32.17 ± 2.24%) attracted significantly more females than 4V2MP and NMBA. The blend attracted more female flies than the minor compounds. The minor compounds might be synergistic to major compounds, however further study is needed to clarify the effectiveness of the five active compounds. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:36:12Z (GMT). No. of bitstreams: 1 ntu-100-R97632010-1.pdf: 9632418 bytes, checksum: 2896682ea8c25f06956678fccfb4dc03 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員審定書 i
誌謝 ii 中文摘要 iii 英文摘要 iv 目錄 vi 表目錄 viii 圖目錄 ix 附錄 x 前言 1 前人研究 4 一、果實蠅之求偶及交配行為 4 二、果實蠅之性費洛蒙 5 三、甲基丁香油 7 四、利用氣相層析儀-觸角電圖 (GC-EAD) 技術鑑定化合物的生物活性 8 材料方法 10 一、試驗昆蟲 10 二、雄蟲氣味誘引雌蟲試驗 10 三、雄蟲氣味收集及化學分析 11 四、使用氣相層析儀-觸角電圖 (GC-EAD) 技術分析活性成分 11 I.電極和觸角之準備 11 II.氣相層析儀-觸角電圖 (GC-EAD) 之試驗流程 12 III.具生物活性之化合物的準備 13 五、具活性之化合物對雌蟲誘引效力試驗 13 I.小帳棚內以透明塑膠陷阱進行誘引試驗 14 II.網室內以黏蟲紙進行誘引試驗 14 六、統計分析方法 14 結果 15 一、雄蟲氣味誘引雌蟲試驗 15 二、雄蟲氣味之化學分析 15 三、使用氣相層析儀-觸角電圖 (GC-EAD) 技術分析活性成分 16 四、具活性之化合物對雌蟲誘引效力試驗 16 I.小帳棚內以透明塑膠陷阱進行誘引試驗 17 II.網室內以黏蟲紙進行誘引試驗 17 討論 18 一、雄蟲氣味誘引雌蟲試驗 18 二、雄蟲氣味之化學分析及活性成分鑑定 18 三、具活性之化合物對雌蟲誘引效力試驗 19 參考文獻 21 表 29 圖 30 附錄 39 表 1.東方果實蠅ME-fed雄蟲萃取液之已知化合物組成 29 圖 1.未交配之東方果實蠅雌蟲被雄蟲氣味誘引比率 30 2.東方果實蠅雄蟲萃取液之氣相層析圖 31 3.東方果實蠅雌蟲對ME-fed雄蟲萃取液的GC-EAD圖譜 32 4.東方果實蠅雌蟲對N-(3-methylbutyl) acetamide (NMBA) 的GC-EAD圖譜 33 5.東方果實蠅雌蟲對 4-vinyl-2-methoxy-phenol (4V2MP) 的GC-EAD圖譜 34 6.東方果實蠅雌蟲對eugenol的GC-EAD圖譜 35 7.東方果實蠅雌蟲對methyl eugenol (ME) 的GC-EAD圖譜 36 8.各活性化合物在小帳棚內對東方果實蠅雌蟲之平均誘引率 37 9.各活性化合物在溫室內對東方果實蠅雌蟲之平均誘引率 38 附錄 1.供試已商品化有效誘引成分的種類、純度及來源 39 2.雄蟲氣味誘引雌蟲試驗之Y型飛行通道裝置圖 40 3.氣相層析儀-觸角電圖 (GC-EAD) 之蟲體固定與電極相關位置圖 41 4.具活性之化合物誘引試驗中使用之白色黏紙 42 5.東方果實蠅雄蟲氣味誘引雌蟲試驗期間之環境參數 43 6.N-(3-methylbutyl) acetamide (NMBA) 的質譜圖 44 7.4-vinyl-2-methoxy- phenol (4V2MP) 的質譜圖 45 8.eugenol的質譜圖 46 9.methyl eugenol (ME) 的質譜圖 47 | |
| dc.language.iso | zh-TW | |
| dc.subject | 氣相層析儀-觸角電圖 | zh_TW |
| dc.subject | 東方果實蠅 | zh_TW |
| dc.subject | 性費洛蒙 | zh_TW |
| dc.subject | 甲基丁香油 | zh_TW |
| dc.subject | sex pheromone | en |
| dc.subject | methyl eugenol | en |
| dc.subject | Oriental fruit fly | en |
| dc.subject | GC-EAD | en |
| dc.title | 東方果實蠅雄蟲萃取物之誘雌成分鑑定與生物檢測 | zh_TW |
| dc.title | Identification and bioassay of female attractants extracted from the male oriental fruit fly (Bactrocera dorsalis (Hendel)) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李後晶,賀孝雍,陳健忠 | |
| dc.subject.keyword | 東方果實蠅,甲基丁香油,性費洛蒙,氣相層析儀-觸角電圖, | zh_TW |
| dc.subject.keyword | Oriental fruit fly,methyl eugenol,sex pheromone,GC-EAD, | en |
| dc.relation.page | 47 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-07-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
| 顯示於系所單位: | 昆蟲學系 | |
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