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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林宗賢 | |
| dc.contributor.author | Kwei-Duan Chang | en |
| dc.contributor.author | 張桂端 | zh_TW |
| dc.date.accessioned | 2021-06-13T06:11:44Z | - |
| dc.date.available | 2007-02-24 | |
| dc.date.copyright | 2006-02-24 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-02-20 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34498 | - |
| dc.description.abstract | 紅龍果(Hylocereus spp.)與黃龍果(Selenicereus megalanthus)是可供果實食用的攀附性仙人掌。近二十年來台灣大量引進紅龍果，種類繁多，包含H. undatus 、H. polyrhizus 、H. costaricensis，果肉顏色分別為白色、紫紅色、紅色，由於種間、屬間無雜交障礙，導致現有品系繁雜。本研究以RAPD分析現有攀附性仙人掌品系，並輔以形態性狀來探討品系間遺傳歧異度與親緣關係。自188個逢機引子組中，篩選出39個引子，對78個攀附性仙人掌品系進行PCR反應，共增幅出398個條帶，其中181個是多型性條帶，占總條帶數的45.5%。經群集分析建立聚類樹狀圖，遺傳相似度在0.58時，Hylocereus 屬大致可區分為三群，H. undatus、H. polyrhizus、H. costaricensis、以及一個形態很特殊的品系，分群結果與形態分類結果相符合。群內有些品系兼具其他種的形態特徵及專一性條帶，推測是種間雜交後代。然而，S. megalanthus與Hylocereus 屬的遺傳相似度達0.40且與Hylocereus spp.具有217個共有條帶，有可能是屬間雜交親合的分子標誌佐證。此外，RAPD分析結果篩選出具種與品系的專一性條帶，可供雜交親緣追溯或品系鑑定之用。 | zh_TW |
| dc.description.abstract | Abstract
In the last two decades, a great number of lines in Hylocereus were introduced into Taiwan including lines in H. undatus, H. polyrhizus, and H. costaricensis. The most distinct character among H. undatus, H. polyrhizus, and H. costaricensis are their flesh color, white, purple and dark red, respectively. Due to interspecific and intergeneric hybridization, the Hylocereus collection in Taiwan is very diverse. The objective of this study was to analyze the genetic relationship among 76 Hylocereus, 1 Selenicereus, and 1 intergeneric line between Hylocereus and Selenicereus through RAPD and morphological characters. In the RAPD analysis, 39 selected primers generated 181 (45.5%) reproducible polymorphic and 217 (54.5%) monomorphic bands. The dendrogram generated by cluster analysis based on 181 polymorphic bands resembled the morphological classification. At similarity coefficient 0.58, the Hylocereus genera were diverted into three main groups and one individual line. The morphological characters of the three groups resembled H. undatus, H. polyrhizus, H. costaricensis, respectively. However, some of the lines within each group were observed with some specific morphological characters and species-specific PCR products that belonged to another group. These lines were highly suspected to be the offspring of interspecific hybrids. The similarity coefficient between S. megalanthus and Hylocereus was 0.40. Such high similarity might be the molecular indication of low incongruity while doing intergeneric cross. Moreover, generic specific PCR products were identified and could be used to trace for the genetic background of some special lines in the collection. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T06:11:44Z (GMT). No. of bitstreams: 1 ntu-95-R92628134-1.pdf: 1086622 bytes, checksum: 994259901e5d4c20180ba5e5d8732a8a (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 中文摘要…………………………………………………………………1 Abstract……….…………………………………………………………..2
前言……………………………..………………………………………..3 前人研究………………………………..………………………………..5 一、攀附性仙人掌學名與分類………………………………………5 二、分子標誌的原理…………………………..……………………..8 三、群集分析與主成份分析原理…………………………………....9 四、RAPD分子標誌的應用……………………………………….....10 (一) 親緣地理的應用…………………………………………..10 (二)親緣關係分析的應用……………………………………....10 (三)品種鑑定的應用……………………………………………11 (四)雜交種檢測的應用…………………………………………11 五、RAPD分子標誌在攀附性仙人掌的應用……………………11 材料與方法…………………………………..………………………...13 一、植物材料………………………………………………………13 二、DNA抽取……………………………………………………..13 三、DNA品質檢定與定量………………………………………..14 四、引子篩選及PCR反應…………………………………………14 五、統計資料分析…………………………………………………15 結果……………………………………………………..………………17 一、不同部位對DNA產量之影響……………………………….17 二、攀附性仙人掌種類與品系間的親緣關係……………………17 (一) 引子篩選與屬、種及品系共有或專一性條帶…………17 (二) 遺傳相似度與群聚關係…………………….………….18 (三) 主成份分析品系間親緣關係………………….……….19 (四) 遠緣雜交的證據………………………………………..20 1. 屬間雜交的形態與分子證據……………………….20 2. 種間雜交的形態與分子證據……………………….20 3. 其他………………………………………………….22 討論………………………………………………………………..23 一、DNA產量之差異…………………………………………….23 二、攀附性仙人掌種類與品系間的親緣關係……………………23 (一) S. megalanthus在植物學分類的定位………..…….....24 (二) 以形態佐證分群結果……………………………….….24 (三) 遠緣雜交鑑定的分子與形態佐證……………………..25 1. 屬間雜交的鑑定…………………………………….25 2. 種間雜交的鑑定…………………………………….25 3. 其他………………………………………………….26 參考文獻…………………………….………………………………….28 表目次 表一、78個攀附性仙人掌參試品系之來源………………..…………32 表二、不同部位的攀附性仙人掌DNA含量及品質比較…………….33 表三、78個攀附性仙人掌以RAPD分析具多型性的39個引子序列及 條帶數..……..……………………………………………………..34 表四、攀附性仙人掌種類及品系的共有條帶與專一性條帶一覽表…36 表五、S. megalanthus (編號36)、屬間雜交品系(編號78)、編號2品 系與三個Hylocereus spp.間的平均遺傳相似度…………..……..37 表六、攀附性仙人掌品系與S. megalanthus、Hylocereus spp.間共有專 一性條帶數目..................................................................................38 表七、部分攀附性仙人掌品系與Hylocereus spp.間的共有專一性條帶 數目………………………………………………………………..39 表八、部分參試品系具有不同屬與種之形態特徵……………….…..41 表九、8個疑似種間雜交後代與Hylocereus spp.的相似度………….43 圖目次 圖一、78個攀附性仙人掌品系以181個多型性條帶經群集分析之聚 類樹狀圖…………………………………………………………..44 圖二、78個攀附性仙人掌品系以181個多型性條帶的主成份分析圖 ……………………………………………………………………..45 圖三、78個攀附性仙人掌參試品系以OPM-18所增幅出的RAPD圖譜 ……………………………………………………………………..46 圖四、編號78品系的形態特徵………………………………..………47 圖五、編號2品系的形態特徵………………………………………...47 | |
| dc.language.iso | zh-TW | |
| dc.subject | Selenicereus | zh_TW |
| dc.subject | 攀附性仙人掌 | zh_TW |
| dc.subject | RAPD | zh_TW |
| dc.subject | Hylocereus | zh_TW |
| dc.subject | RAPD | en |
| dc.subject | Selenicereus | en |
| dc.subject | Hylocereus | en |
| dc.subject | calimbing cacti | en |
| dc.title | 攀附性仙人掌品系之親源分析 | zh_TW |
| dc.title | Genetics relationships among Hylocereus and Selenicereus lines based on RAPD markers | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 顏昌瑞,楊雯如 | |
| dc.contributor.oralexamcommittee | 柳建安 | |
| dc.subject.keyword | 攀附性仙人掌,RAPD,Hylocereus,Selenicereus, | zh_TW |
| dc.subject.keyword | calimbing cacti,RAPD,Hylocereus,Selenicereus, | en |
| dc.relation.page | 47 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-02-20 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
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