14-3-3基因家族在斑馬魚胚胎發育之表現及其過度表現所引發之神經纖維生長

Yu-Hua Lin; 林玉華

請用此 Handle URI 來引用此文件： http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34337

完整後設資料紀錄

DC 欄位	值	語言
dc.contributor.advisor	黃銓珍(Chang-Jen Huang)
dc.contributor.author	Yu-Hua Lin	en
dc.contributor.author	林玉華	zh_TW
dc.date.accessioned	2021-06-13T06:03:43Z	-
dc.date.available	2008-07-03
dc.date.copyright	2006-07-03
dc.date.issued	2006
dc.date.submitted	2006-06-19
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dc.identifier.uri	http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34337	-
dc.description.abstract	14-3-3是一種酸性蛋白，大小約25-30kDa，主要存在於腦部和神經性統中。它的功能廣泛，包含代謝，細胞週期的控制，訊息傳導，細胞凋亡，蛋白運送，轉錄，壓力反應及惡性轉型等。目前已知它和超過兩百種的受質結合，涵蓋細胞內幾乎所有的反應。本實驗根據其它物種比對所得之14-3-3序列來選殖斑馬魚14-3-3基因，並且收集資料庫中之其它已知的斑馬魚14-3-3序列，最後得到九種斑馬魚14-3-3蛋白家族成員，包括ζ，θ，β1，β2，β3，η，γ，ε1，以及ε2。經過胺基酸排序分析，親緣演化樹分析，基因結構分析，以及成對比較之結果，推論ζ，θ，β1，β2，及β3親緣關係較接近，屬於第一群；η和γ為第二群；ε1及ε2則為第三群。利用全胚胎原位雜交法分析斑馬魚14-3-3家族之mRNA在受精後六小時到一百二十小時的胚胎表現分佈位置，結果發現它們在腦部之表現量最多，其次則是視網膜，鰓，以及腸道。將斑馬魚14-3-3的九個基因以顯微注射方式表現在斑馬魚早期胚胎中，結果在受精後48小時，只有14-3-3 β3可觀察到明顯的神經纖維生長現象。將14-3-3 β3作R56A及R60A雙突變以破壞14-3-3和受質的結合，或是將14-3-3 β3與蛋白質去磷酸酶PP2A一同表現以使受質去磷酸化，均可有效抑制神經纖維生長的情形。而隨著MEK (MAPKK) 抑制劑PD98059的增加，神經纖維生長的抑制越明顯。將斑馬魚的Rho GTPase家族中的Rac-1及Cdc42均作T17N突變，和14-3-3 β3一同注射到斑馬魚胚胎中，也是隨著注射劑量增加，抑制神經纖維生長的效果越明顯。故由以上結果，推測斑馬魚14-3-3 β3和受質結合後，是經由MEK及MAPK路徑而使神經纖維生長，然而Rac-1及Cdc42在路徑中作用的位置則尚未明瞭。	zh_TW
dc.description.abstract	14-3-3 family proteins are adaptor proteins that specifically bind to a discrete phosphoserine or phosphothreonine motif in the substrate proteins. They play important roles in metabolism, cell-cycle control, signal transduction, apoptosis, protein trafficking, transcription, stress responses, and malignant transformation. They are found only in eukaryotes. In this study, I cloned and characterized 9 genes encoding nine members of zebrafish 14-3-3 family, designated theta (θ), zeta (ζ), beta1 (β1), beta2 (β2), beta3 (β3), eta (η), gamma (γ), epsilon1 (ε1), epsilon2 (ε2). Amino acid sequence alignment and phylogenetic tree analysis indicated that all zebrafish 14-3-3 members can be divided into three group. The ζ, θ, β1, β2 and β3 belong to Group I, while η and γ Group II. In addition, ε1 and ε2 are the third. Using whole-mount in situ hybridization to analyze the mRNA expression profile of zebrafish 14-3-3 family from 6 hpf to 120 hpf, the signal was found primarily in the brain while moderate in retina, gill and gut. Expression of GFP-fusion protein of each isoform of zebrafish 14-3-3 family by a neuron-specific HuC promoter in zebrafish embryos indicated that only 14-3-3-beta3 isoform could promote neurite outgrowth. A double mutant of 14-3-3-beta3 (R56A and R60A) abolished this effect. Coinjection of pHuC-14-3-3-beta3-HuC-GFP with protein phosphatase 2A (PP2A), Cdc42 dominant negative form (Cdc42-T17N), Rac1 dominant negative form (Rac1-T17N) and MAPKK inhibitor PD98059, respectively, reduced the neurite outgrowth induced by 14-3-3-beta3.	en
dc.description.provenance	Made available in DSpace on 2021-06-13T06:03:43Z (GMT). No. of bitstreams: 1 ntu-95-R93b46003-1.pdf: 2304936 bytes, checksum: c0b576bb3a8c39053ef2c7b47b8fdd88 (MD5) Previous issue date: 2006	en
dc.description.tableofcontents	目錄中文摘要…………………………………………………………...…....................... i 英文摘要 (Abstract)…………………………………………….…......................... ii 縮寫表………………………………………………………….......…....................... iii 目錄…………………………………………………………...........…....................... iv 表目錄……………………………………………………………………………….. vi 圖目錄……………………………………………………………………………….. vii 壹、序言…………………………………………………………….......................... -1- 一、14-3-3的基本背景…………………………………………………………. -1- 二、14-3-3的蛋白質結構………………………………………………………. -2- 三、14-3-3蛋白的作用機制……………………………………………………. -4- 四、14-3-3目標結合的調控……………………………………………………. -7- 五、不同物種間的14-3-3蛋白家族…………………………………………… -11- 六、14-3-3蛋白家族在不同組織的表現分布…………………………………. -11- 七、14-3-3對於人類神經性及腫瘤相關疾病的影響…………………………. -12- 八、Rho GTPase 家族……………………………………………………...…... -14- 九、研究目的及策略…………………………………………………………… -15- 貳、實驗材料與方法……………………………………………………………….. -17- I. 實驗材料……………………………………………………………....……… -17- II. 實驗方法…………………………………………………………………….. -17- 一、斑馬魚14-3-3基因之選殖………………………………………………… -18- 二、斑馬魚胚胎之顯微注射……………………………………………………. -23- 三、斑馬魚14-3-3 mRNA在早期胚胎發育之表現：RNA全胚胎原位雜交 (RNA whole-mount in situ hybridization) …………………………………. -25- 參、結果………………………………………………………….............................. -32- I. 斑馬魚14-3-3基因家族序列………………………………………………... -32- 一、選殖及分析斑馬魚14-3-3基因家族……………………………………… -32- 二、斑馬魚14-3-3基因家族成員的親緣演化關係…………………………… -33- II. 斑馬魚14-3-3在不同胚胎發育時期的表現位置及表現量……………….. -34- 一、斑馬魚14-3-3 ζ mRNA的表現分布………………………………………. -34- 二、斑馬魚14-3-3 θ mRNA的表現分布………………………………………. -35- 三、斑馬魚14-3-3 β1 mRNA的表現分布…………………………………….. -36- 四、斑馬魚14-3-3 β2 mRNA的表現分布…………………………………….. -36- 五、斑馬魚14-3-3 β3 mRNA的表現分布…………………………………….. -37- 六、斑馬魚14-3-3 η mRNA的表現分布……………………………................ -38- 七、斑馬魚14-3-3 γ mRNA的表現分布……………………………................. -39- 八、斑馬魚14-3-3 ε1 mRNA的表現分布…………………………………….. -40- v 九、斑馬魚14-3-3 ε2 mRNA的表現分布…………………………………..… -40- 十、綜合斑馬魚14-3-3各isoform的表現分布……………………………….. -41- III. 斑馬魚14-3-3基因家族影響神經纖維生長及其相關基因………………. -42- 一、過度表現斑馬魚14-3-3基因家族…………………………….…………… -42- 二、斑馬魚14-3-3基因家族過度表現所導致神經纖維生長之比例………… -43- 三、抑制斑馬魚14-3-3 β3 和受質的結合會減少神經纖維的生長………….. -44- 四、MEK抑制劑可突變減少斑馬魚14-3-3 β3所導致之神經纖維生長的情形…………………………………………………………………………… -45- 五、Cdc42及Rac-1的突變會抑制斑馬魚14-3-3 β3所導致之神經纖維生長的情形…………………………………………………………………….... -45- 肆、討論…………………………………………………………………………….. -47-
dc.language.iso	zh-TW
dc.title	14-3-3基因家族在斑馬魚胚胎發育之表現及其過度表現所引發之神經纖維生長	zh_TW
dc.title	Gene expression of 14-3-3 family during zebrafish development and their overexpressions lead to the promotion of neurite outgrowth	en
dc.type	Thesis
dc.date.schoolyear	94-2
dc.description.degree	碩士
dc.contributor.oralexamcommittee	黃鵬鵬(Pung-Pung Hwang),黃聲蘋(Sheng-Ping L. Hwang)
dc.subject.keyword	14-3-3,斑馬魚,神經纖維,	zh_TW
dc.subject.keyword	14-3-3,zebrafish,neurite outgrowth,	en
dc.relation.page	102
dc.rights.note	有償授權
dc.date.accepted	2006-06-20
dc.contributor.author-college	生命科學院	zh_TW
dc.contributor.author-dept	生化科學研究所	zh_TW
顯示於系所單位：	生化科學研究所

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