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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34187完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林中天 | |
| dc.contributor.author | Wen-Chih Tsai | en |
| dc.contributor.author | 蔡文智 | zh_TW |
| dc.date.accessioned | 2021-06-13T05:57:25Z | - |
| dc.date.available | 2006-07-05 | |
| dc.date.copyright | 2006-07-05 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-06-28 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/34187 | - |
| dc.description.abstract | 本實驗目的為評估注射型藥物lidocaine（LDC）與methylprednisolone（MP）是否對於視網膜缺血性病變有神經保護效果。經高眼內壓誘發視網膜缺血，將SD大鼠分成三組：包括IR（ischemia-reperfusion）無治療之對照組（n=5）、給予LDC（n=7）與MP（n=7）兩組IR治療實驗組。大鼠眼前房以30號針頭穿刺並連接上含生理食鹽水的點滴袋，將眼內壓提高到130mmHg造成眼底視網膜完全缺血的狀態持續45分鐘。LDC治療實驗組在缺血前30分鐘靜脈注射lidocaine 1.5mg/kg的劑量，隨即以2mg/kg/hr的速率持續靜脈注射到缺血結束後60分鐘。MP治療實驗組給予兩次methylprednisolone 30mg/kg的靜脈注射，分別在缺血前2分鐘與缺血後馬上給予。傷害評估利用缺血後三天視網膜電波圖來檢查視網膜功能性的改變，以及缺血後七天在組織病理形態計量上視網膜的厚度改變。視網膜電波圖結果顯示a波在IR無治療組下降35.2%±22.3%， LDC組下降49.7%±21.3%，而MP組無顯著差異；b波在無治療組下降81.0%±4.6%，LDC組下降80.7%±5.1%，而MP組僅稍微下降17.6%±13.4%。組織病理結果顯示內層視網膜傷害均比外層強，而內叢狀層與外核層的比值（IPL/ONL ratio）與正常的百分比分析（% of正常），IR無治療組只剩下48.8%，LDC治療組顯示存留程度為80.1%，而MP治療組剩存的百分比可高達96.2%，幾乎沒有下降。結論為MP對於高眼壓造成的視網膜缺血性傷害確有良好的視網膜神經保護效果，包括在視網膜功能及組織病理方面；而LDC的視網膜保護效果僅表現在病理的型態計量上，在視網膜電波圖檢查並未顯示保護效果。 | zh_TW |
| dc.description.abstract | Purpose: To evaluate the neuroprotective effects of intravenous (IV) lidocaine (LDC) and methylprednisolone (MP) against the retinal ischemia-reperfusion (IR) insult. Methods: SD rats were divided into 3 groups, the IR control (n=5), LDC (n=7) and MP (n=7). Saline was infused into the anterior chamber using 30-gauge needle, which created a high intraocular pressure than blood pressure and lasted for 45 min. LDC bolus (1.5mg/kg) was IV injected 30 min before ischemia and then a constant rate infusion (CRI) with 2mg/kg/hr was given until 60 min after reperfusion. MP bolus (30mg/kg) was IV administered twice at 2 min before and immediately after ischemia, respectively. The damage to retinal function was evaluated by electroretinogram (ERG) 3 days after ischemia and by morphometrical histology analysis 7 days after ischemia. Results: The functional analysis of the retina by ERG revealed a 35.2% reduction of a-wave in the IR control group, 49.7% reduction in the LDC group but no significant change in the MP group. The b-wave reduction showed 81.0% in the IR control, 80.7% in the LDC group and 17.6% in the MP group. In the morphometrical histology, the retinal inner plexiform layer/outer nuclear layer (IPL/ONL) ratio was reduced following IR damage compared with that in the normal control. The IPL/ONL ratio was reduced to 48.8% in the IR control group, 80.1% in the LDC group and 96.2% in MP group. Conclusions: The MP showed significantly good neuroprotective effects on retinal IR injury, and the LDC showed moderate neuroprotective effects demonstrated in histology but not in retinal function. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T05:57:25Z (GMT). No. of bitstreams: 1 ntu-95-R92629029-1.pdf: 1263499 bytes, checksum: 9d2d1320304e929de968a29d2fb2ad12 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 目錄
中文摘要 1 英文摘要 3 第一章 緒言 5 第一節 青光眼與視網膜缺血性病變 5 第二節 視網膜神經保護概念與治療 5 第三節 研究目的 7 第二章 文獻探討 8 第一節 青光眼簡介 8 一、青光眼的定義與分類 8 二、犬貓青光眼發生狀況 10 三、犬貓青光眼治療簡介 10 第二節 青光眼與視網膜缺血再灌流性病變 11 一、視網膜缺血性病變形態與動物模式 11 二、視網膜缺血性病變階段性評估 12 三、閃光式視網膜電波圖評估 14 四、組織病理評估 15 第三節 神經保護理論與治療策略 18 一、視網膜缺血神經傷害機制 18 二、神經保護策略及路徑 21 三、再灌流性病變與Lidocaine治療應用 24 四、Methylprednisolone的治療策略 29 第三章 實驗材料與方法 31 第一節 實驗動物 31 第二節 實驗材料與分組 31 第三節 缺血再灌流模式 31 第四節 神經保護藥物的給予 32 第五節 視網膜電波圖的記錄與分析 33 第六節 組織病理學切片評估 35 第七節 統計與分析方法 36 第四章 實驗結果 37 第一節 視網膜電波圖電生理分析結果 37 一、視網膜電波圖a與b波呈像 37 二、視網膜電波圖b波下降幅度分析比較 43 三、視網膜電波圖a波下降幅度分析比較 50 四、視網膜電波圖a與b波綜合比較 57 第二節 組織病理形態計量學分析報告 59 一、各層視網膜厚度病變比較 59 二、外核層與內核層的細胞數量比較 66 三、內叢狀層與外核層的比值分析（IPL/ONL ratio） 69 第五章 討論 71 第一節 視網膜缺血性病變模式的選擇 71 第二節 神經保護藥物的選擇 74 第三節 視網膜電波圖討論 76 第四節 組織病理形態計量學的討論 85 第五節 視網膜電波圖與組織病理形態之關聯性 90 第六節 與近年有關視網膜神經保護研究的文獻比較 93 第六章 結論 97 第七章 參考文獻 99 | |
| dc.language.iso | zh-TW | |
| dc.subject | 視網膜 | zh_TW |
| dc.subject | 缺血再灌流病變 | zh_TW |
| dc.subject | 利多卡因 | zh_TW |
| dc.subject | 命得生 | zh_TW |
| dc.subject | 大鼠 | zh_TW |
| dc.subject | methylprednisolone | en |
| dc.subject | rat | en |
| dc.subject | retina | en |
| dc.subject | ischemia-reperfusion injury | en |
| dc.subject | lidocaine | en |
| dc.title | Lidocaine與Methylprednisolone對於大鼠視網膜缺血再灌流病變模式的神經保護效力 | zh_TW |
| dc.title | The Neuroprotective Effects of Lidocaine and Methylprednisolone in a Rat Model of Retinal Ischemia-Reperfusion Injury | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 葉力森,劉振軒,詹東榮,張芳嘉 | |
| dc.subject.keyword | 缺血再灌流病變,利多卡因,命得生,大鼠,視網膜, | zh_TW |
| dc.subject.keyword | ischemia-reperfusion injury,lidocaine,methylprednisolone,rat,retina, | en |
| dc.relation.page | 106 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-06-29 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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