高分子材料表面改質對血小板儲存條件之研究

Abstract

本研究是利用乾式法將高分子聚氯乙烯、聚乙烯醇與乙烯-乙烯 醇共聚合物製成薄膜後，用化學改質的方法將薄膜表面接枝胺基，分 別為聚氯乙烯以二胺類改質，聚乙烯醇與乙烯-乙烯醇共聚合物以二 異氰酸鹽將表面之氫氧基接枝改質成胺基，再分別利用Micro-ATR 與Orange II 來分析薄膜改質前後官能基的變化與其接枝量。 同時將左旋-精氨酸作為血小板儲存時的添加劑，來比較血小板 儲存時材料與添加劑對於血小板之影響，所利用的測試方法分別為細 胞活性的MTT 與LDH、血小板凝集能力的凝集測試、血小板活化、 存活與死亡的抗體標定與血小板貼附與型態改變的SEM 照片，利用 這些方法來檢定血小板在儲存時的各種狀況。 血小板在經由儲存測試後可以發現經化學改質後的高分子薄膜 可以在初期利用其較為親水的表面提供一個血小板較不易貼附的環 境，並且能維持血小板較好的活性與功能，只是此項差異性會隨著儲 存時間的延長而逐漸消失，而將左旋-精氨酸作為添加劑則可將材料 間的差異性拉大並延長血小板活性保持的時間，並且將左旋-精氨酸 基接枝在乙烯-乙烯醇共聚合物上並加入左旋-精氨酸作為添加劑可以 得到最好的實驗結果。

We fabricated poly(vinyl chloride) 、poly(vinyl alcohol) and poly(ethylene-co-vinyl alcohol) polymer membranes by the method of dry processing. Diamine was grafted on the PVC membrane surface by the chemical reaction with the chloride groups. Diisocyanate was grafted on EAVL and PVA membrane surfaces by chemical reaction with the hydroxyl groups. Afterwards, amino group were grafted on these membranes. Also, we analyzed the surface properties of these membranes by means of Micro-ATR、Orange II salt and contact angles. To investigate the platelet storage condition and its qualities with diff. polymer membranes and L-Arginine effects, we used the cell viability method；MTT and LDH、platelet aggregation test、platelet surface marker labeling assay with flow cytomerty, clouding P-Selectin、GpIIb-IIIa complex and Survival-Apoptosis ratio, and platelet adhesion and shape change using take SEM picture. Post-storage platelet were shown the less adhesion and better qualities maintain on the modified membranes, we believed that course with the modified membrane were to provide more hydrophilic condition that can inhibit platelet adhesive on the polymer membranes. When we used L-Arginine to regard as the storage addition solution, we can get more better results on each experimental groups. But L-Arginine effect were lost after 3days. In order to solve this problem, we designed the membrane；EVAL grafting the L-Arginine on the membrane surface, the experiment result were shown the most less platelet adhesion number and best qualities maintain. So, we believe the membrane modification and additive L-Arginine solution to regard as addition solution can effectively improve platelet storage condition and its storage qualities.