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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡偉博(Wei-bor Tsai) | |
dc.contributor.author | Yen-Chung Ting | en |
dc.contributor.author | 丁彥中 | zh_TW |
dc.date.accessioned | 2021-06-13T05:44:03Z | - |
dc.date.available | 2006-07-17 | |
dc.date.copyright | 2006-07-17 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-14 | |
dc.identifier.citation | References
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33634 | - |
dc.description.abstract | 本研究的目的在於探討材料表面的奈米結構對於細胞之貼附、定
向的影響。本實驗將骨母細胞培養在具有奈米級直溝圖案的矽晶片表面上(溝寬為90、150、250、340、500 nm),經過不同的培養時間,再利用電子顯微鏡觀察骨母細胞在各種奈米表面上貼附的情況,並使用軟體分析細胞形態。 我們發現直到四小時後,才有明顯差別。大體而言,細胞在溝寬 大於150 nm 的表面上延伸度比在溝寬90 nm 的表面高;同時細胞沿 著溝向貼附的比例,亦是在溝寬大於150 nm 的表面上較高,顯示細 胞對於寬溝的表面反應較快。不過在一天後,各種溝寬大小的表面上的細胞均已有類似的延伸度,且幾乎都沿溝向排列,彼此之間無明顯差異。此外透過共軛焦螢光顯微鏡,亦觀察到在直溝地形的表面上,細胞的F-actin 會沿溝向平行排列,同時細胞核也會沿溝排列並且形態亦稍有拉長。 另外也使用預吸附fibronectin(Fn)的直溝矽晶片培養骨母細胞。相較於無預吸附Fn 的表面,預吸附Fn 的直溝表面上細胞較不沿溝排列,形態也較不拉長,貼附面積較大,細胞核也較不沿溝排列。雖然短時間內細胞形態會受Fn 所影響,但長時間後細胞形態仍受直溝地形強烈控制。 | zh_TW |
dc.description.abstract | Cells are known to respond to topographic cues in their natural nanometrical environment. The aim of this study is to investigate the influences of surface nanotopography on cell adhesion and orientation. Osteoblast-like cells (MG63) were cultured on a series of nanogrooved silicon wafer (90, 150, 250, 340 and 500 nm in width). At specific time point, the images of cells on different surfaces were captured by scanning electron microscopy, and cell morphology was analyzed by image software.
Initially, cells adhering on surfaces with wider grooves showed more elongated shape. The proportion of cells which aligned parallel to grooves was higher on surfaces with wider grooves. Surfaces with wider grooves promote cells to appear quicker respond of elongation and alignment. F-actin and nuclei aligned with grooves on patterned surfaces. The elongation of nuclei on patterned surfaces was also observed. Besides, osteoblast-like cells were cultured on grooved surfaces pre-adsorbed with Fibronectin (Fn). Compared to surfaces without Fn preadsorption, cells displayed larger spreading area, less elongated appearance and less isotropic alignment on grooved surfaces with Fn pre-adsorption. The proportion of aligned nuclei also decreased on surfaces with Fn preadsorption. Cell morphology could be affected enormously by nano-grooves even if grooves were coated densely with adhesion-associated protein, Fn. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T05:44:03Z (GMT). No. of bitstreams: 1 ntu-95-R93524020-1.pdf: 8876174 bytes, checksum: 2a621c72a7738a71636abc3885755aa4 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 第一章 緒論 1
1.1. 研究背景 1 1.2. 表面地形對細胞形態之影響 2 1.3. 表面地形對細胞移動與功能之影響 3 1.4. 骨母細胞(Osteoblast)簡介 6 1.5. 纖維黏接蛋白(Fibronectin)簡介 8 1.6. 研究動機 9 1.7. 研究架構 10 1.8. 研究目的 10 第二章 實驗藥品、儀器與方法 11 2.1 實驗藥品 11 2.1-1 矽晶片表面清潔 11 2.1-2 細胞培養 11 2.1-3 掃瞄式電子顯微鏡攝影之前處理 12 2.1-4 細胞骨骼與細胞核之螢光染色 12 2.1-5 純化Fibronectin 12 2.1-6 Fibronectin電泳 13 2.2 實驗儀器與耗材 13 2.2-1. 實驗儀器 13 2.2-2. 實驗耗材 14 2.3 溶液配製 15 2.4 實驗方法 18 2.4-1. 矽晶片表面清潔 18 2.4-2. 細胞培養 19 2.4-3. 細胞植佈 19 2.4-4. 掃瞄式電子顯微鏡攝影之前處理 19 2.4-5. 細胞貼附形態分析 20 2.4-6. 細胞骨骼染色 21 2.4-7. 分離與純化Fibronectin 21 2.4-8. 細胞於預吸附Fibronectin之直溝矽晶片表面之貼附 22 2.4-9. Fibronectin電泳 23 2.4-10. 統計分析 23 第三章 奈米級直溝地形表面對於骨母細胞的貼附形態之影響 29 3.1. 實驗結果 29 3.1-1. 細胞貼附形態 29 3.1-2. 細胞貼附面積 30 3.1-3. 細胞延伸度(Elongation) 30 3.1-4. 細胞寬度 31 3.1-5. 細胞排列(Alignment) 32 3.1-6. F-actin、vinculin之排列與分佈 32 3.1-7. 細胞核之延伸與排列 33 3.2. 討論 33 第四章 骨母細胞在預吸附Fibronectin之奈米級直溝地形表面之貼附行為 55 4.1. 實驗結果 55 4.1-1. Fibronectin之分離純化與電泳 55 4.1-2. 細胞貼附形態 56 4.1-3. 細胞貼附面積 57 4.1-4. 細胞延伸度(Elongation) 57 4.1-5. 細胞寬度 58 4.1-6. 細胞排列(Alignment) 59 4.1-7. F-actin之排列與分佈 59 4.1-8. 細胞核之延伸與排列 60 4.2. 討論 60 第五章 結論 83 | |
dc.language.iso | zh-TW | |
dc.title | 奈米級直溝地形對骨母細胞貼附形態之影響 | zh_TW |
dc.title | Influence of Nanoscale Grooved Surface on Osteoblast-like Cell Morphology | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡曉雯(Shiao-Wen Tsai),孫一明(Yi-Ming Sun) | |
dc.subject.keyword | 骨母細胞,奈米,直溝,地形,形態, | zh_TW |
dc.subject.keyword | osteoblast,nanogroove,morphology,topography, | en |
dc.relation.page | 88 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-17 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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