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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 盧國賢 | |
dc.contributor.author | Mei-Jun Yang | en |
dc.contributor.author | 楊梅君 | zh_TW |
dc.date.accessioned | 2021-06-13T04:19:56Z | - |
dc.date.available | 2006-08-03 | |
dc.date.copyright | 2006-08-03 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-24 | |
dc.identifier.citation | Arenas P. 1987. Medicine and magic among the Maka Indians of the Paraguayan Chaco. J Ethnopharmacol 21:279-295.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32950 | - |
dc.description.abstract | 本實驗是以纖維母細胞株 (3T3 cells) 當作實驗細胞,來探討β-Lapachone對於纖維母細胞增生與移行能力的影響,實驗結果如下:1. 經由MTT assay顯示,纖維母細胞株在0.1及1µM β-Lapachone處理後24小時,細胞增生比例分別為126%及133%,與控制組相比,達到顯著差異,由此證明β-Lapachone具有促使纖維母細胞增生的效用;2. 3T3細胞經propidium iodide染色後,藉由流式細胞儀分析細胞週期,結果顯示,細胞經由1µM β-Lapachone處理後3∼24小時,細胞族群在S phase所佔的比例與控制組相比皆有增加,尤其在1µM β-Lapachone處理後9小時與控制組相比達到顯著差異;3. 經由細胞移行測定 (migration assay) 顯示,與控制組比較,1µM β-Lapachone處理後12小時可發現,纖維母細胞移行的能力較佳;4. Western blot分析顯示,3T3細胞經1µM β-Lapachone處理後會活化ERK1/2及p38;5. 使用ERK抑制劑或p38抑制劑 (SB203580) 與1µM β-Lapachone共同處理細胞後顯示,經1µM β-Lapachone處理後所導致的細胞增生與加快移行受到抑制;6. 動物實驗初步結果顯示:經內含100µM β-Lapachone的凡士林軟膏處理後,實驗動物傷口癒合的速度較控制組快。
綜合以上結果顯示,β-Lapachone在傷口癒合的過程中扮演著重要的角色,其主要機制是藉由開啟ERK1/2及p38訊息傳遞路徑而促使纖維母細胞增生及加快移行,進而達到促進傷口癒合的目的。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:19:56Z (GMT). No. of bitstreams: 1 ntu-95-R93446004-1.pdf: 2035429 bytes, checksum: 5ce4a3773120d42c8fe54d3b5c48519f (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 中文摘要 (3)
英文摘要 (4) 縮寫 (6) 一、 緒言 (Introduction) (7) 二、 目的 (Purpose) (14) 三、 實驗材料與方法 (Materials and Methods) (15) 四、 結果 (Results) (21) 五、 討論 (Discussion) (25) 六、 結論 (Conclusion) (32) 七、 未來擬繼續進行之研究工作 (Further work) (33) 八、 參考文獻 (Reference) (34) 九、 圖表 (Figures ) (39) | |
dc.language.iso | zh-TW | |
dc.title | β-Lapachone對於纖維母細胞株增生與移行能力的影響 | zh_TW |
dc.title | Effects of β-Lapachone on the proliferation and migration in 3T3 cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 錢宗良,周逸鵬 | |
dc.subject.keyword | β-Lapachone,纖維母細胞,增生,移行,傷口癒合, | zh_TW |
dc.subject.keyword | β-Lapachone,fibroblast,proliferation,migration,wound healing, | en |
dc.relation.page | 57 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-24 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 解剖學研究所 | zh_TW |
顯示於系所單位: | 解剖學暨細胞生物學科所 |
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