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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曾四恭 | |
dc.contributor.author | Shu-Yu Huang | en |
dc.contributor.author | 黃抒毓 | zh_TW |
dc.date.accessioned | 2021-06-13T04:19:25Z | - |
dc.date.available | 2006-07-27 | |
dc.date.copyright | 2006-07-27 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-23 | |
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New concepts of microbial treatment processes for the nitrogen removal in wastewater. FEMS Microbiology Reviews, 27, 481-492. Schmidt, I., Sliekers, O., Schmid, M., Cirpus, I., Strous, M., Bock, E., Kuenen, J. G., Jetten, M. S. M., 2002. Aerobic and anaerobic ammonia oxidizing bacteria – competitors or natural partners. FEMS Microbiology Ecology, 39, 175-181. Stroo, H. F., T. M. Klein, and M. Alexander. 1986. Heterotrophic nitrification in acid forest soil and by a acid-tolerent fungus. Appl. Environ. Microbiol. 52: 1107-1111. Stouthamer, A. H. 1976. 'Biochemistry and genetics of nitrate reductase in bacteria.' Adv. Microbi. Physiol. 14: 315-375. van Loosdrecht, M. C. M., and Jetten M. S. M. 1998. Microbiological conversions in nitrogen removal. Water science and technology 38(1):1-7. van Niel, E. W. J., K. J. Braber, L. A. Robertson, and J. G. Kuenen. 1992. Heterotrophic nitrification and aerobic denitrification in Alcaligenes faecalis strain TUD. Antonie van Leeuwenhoek 62:231-237. 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Bioresourse, 86, 245-252 石秉鑫。2001。『以固定之好氧脫硝菌應用於同槽硝化脫硝反應之可行性研究』。國立台灣大學環境工程研究所碩士論文,台北。 曹明浙。2003。『同槽硝化脫硝反應去除廢水氨氮之研究』。國立台灣大學環境工程研究所碩士論文,台北。 陳旻谷。2003。『菌株Achromobacter oxylosoxidans同時硝化脫硝之特性研究』。國立台灣大學環境工程研究所碩士論文,台北。 莊隆光。2004。『固定特殊氮細菌進行單槽碳氮去除之研究』。國立台灣大學環境工程研究所碩士論文,台北。 林昊勳。2001。『多段式生物濾床併同去除碳、氮、磷之研究』。國立中央大學環境工程研究所碩士論文,中壢。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32931 | - |
dc.description.abstract | 本研究目的為了解異營硝化脫硝菌Achromobacter oxylosoxidans和Pseudomonas stutzeri對於模擬家庭污水是否具有同時硝化脫硝,及同時碳氮去除之效果。首先利用密閉血清瓶批次試驗確認菌株進行同時硝化脫硝之特性現象,再改以模擬家庭污水連續進流,評估其用於實際廢水處理之可能性,同時找出最適操作條件供實際廢水處理時參考。最後利用分子生物技術來追蹤固定化擔體之菌相變化,確認其長時間應用之可行性。
異營硝化脫硝菌Achromobacter oxylosoxidans和Pseudomonas stutzeri經由實驗證實在模擬家庭污水環境下,具有同時硝化脫硝現象存在,亦可有效利用廢水中之有機碳源,達到除碳之效果。經過固定化後之異營硝化脫硝菌,除有長時間存留於反應槽的能力外,更可提升其在好氧環境下之脫硝能力。 於本實驗負荷範圍內,以混合菌株於總氮濃度= 40 mg/L,COD濃度= 765 mg/L,COD/TN = 19,COD負荷 = 1.147 kg/m3/day時,硝化率為39 % ,總氮去除率為71 % 為較佳之操作條件。 異營硝化脫硝菌於一般家庭污水水質條件下,於總氮濃度= 30 ~ 40 mg/L,COD濃度= 300 ~500 mg/L,總氮去除率雖然可達53 ~ 67 %,然而氨氮殘餘量過高,較難符合氨氮放流水標準10 mg/L,且污泥增殖量較多,造成廢棄污泥處理上之問題。因此,可考慮於系統中添加自營硝化菌,以提升整體硝化效率。 以分子生物技術–DGGE追蹤連續流反應槽內固定化菌株之菌相變化情形,發現經過長時間操作後,異營硝化脫硝菌仍存在於固定化擔體中,顯示固定化作用確實可保有菌株不易流失之優點。 | zh_TW |
dc.description.abstract | This study was to investigate the simultaneous removal of carbon and nitrogen with Achromobacter oxylosoxidans and Pseudomonas stutzeri from synthetically-made domestic wastewater, and to verify the simultaneous nitrification and denitrification(SND). First of all, using a batch experiments to analyze the SND reaction of suspended cells and immobilized cells. Furthermore, continuous-flow test was performed with synthetically-made domestic wastewater use to evaluate the availability of removing nitrogen from wastewater, and to find the optimum operation as a guideline for the treating wastewater as the same time. At the last, DGGE-based investigation was also performed by using immobilized cells to verify the feasibility in the continuous-flow system.
Experiment testified that Achromobacter oxylosoxidans and Pseudomonas stutzeri have the ability to simultaneously remove carbon and nitrogen from synthetically-made domestic wastewater, and also have the appearance of SND. In our researches, in addition to prove the immobilized cells ability of feasibility in the continuous-flow system, nitrification and denitrification are demonstrated efficiently under high DO level. Furthermore, especially for mixed-culture groups which provide 39 % nitrification efficiency, and 71 % total nitrogen removal as T-N= 40 mg/L, COD= 765 mg/L, C/N= 19 for the better operation. Although the immobilized cells can treat synthetically-made domestic wastewater with 53~67 % total nitrogen removal rate, the system produces too much sludge and ammonia residue and achieves the effluent-standard which limits its feasibility in real operations. Hence, autotrophic nitrifiers were suggested to be applied for enhancing the nitrification efficiency. Tracing the strain in the immobilized cells with DGGE method after the continuous-flow test shows that the strain can be conserved in the immobilized cells well and it appears that immobilized technique can successfully maintains the strains from being washed out after long-term operations. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:19:25Z (GMT). No. of bitstreams: 1 ntu-95-R93541106-1.pdf: 1729989 bytes, checksum: 087a2ab0ddade0b8b35eeb7cb2ec86da (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 摘要 I
Abstract III 目錄 IV 表目錄 VIII 圖目錄 X 第一章 前言 1 1-1 研究緣起 1 1-2 研究目的及內容 2 第二章 文獻回顧 4 2-1 自然界中之氮循環 4 2-2 硝化作用(Nitrification) 4 2-2-1 硝化反應 5 2-2-2 影響硝化作用之因素 8 2-3 脫硝作用(Denitrification) 10 2-3-1 脫硝菌 10 2-3-2 生化反應 11 2-3-3 影響脫硝作用之因素 13 2-4 環境中較特殊之氮循環途徑 15 2-4-1 同時硝化脫硝 15 2-4-2 好氧脫硝(Aerobic denitrification) 17 2-4-3 異營硝化(Heterotrophic nitrification) 18 2-5 同時硝化脫硝反應槽 21 2-6 固定化細胞擔體之應用 25 2-7 利用16S rDNA分析菌相結構之相關研究 28 2-7-1 16S rDNA序列比對 28 2-7-2 以變性梯度凝膠電泳(Denaturing gradient gel electrophoresis – DGGE)分析16S rDNA 32 第三章 材料與方法 34 3-1 研究內容 34 3-2 菌種活化與增殖 35 3-2-1 菌種來源 35 3-2-2 菌種活化與增殖 35 3-3 固定化菌株製作與活化 37 3-3-1 固定化菌株製作 37 3-3-2 固定化菌株活化 38 3-4 血清瓶批次試驗 38 3-4-1 血清瓶批次試驗流程 39 3-4-2 懸浮與固定化菌株硝化脫硝功能測試批次試驗 39 3-4-3 菌株於不同基質成份之硝化脫硝功能批次試驗 40 3-4-4 固定化菌株不同碳氮比之批次試驗 41 3-5 固定化菌株之硝化脫硝功能連續流試驗 43 3-5-1 不同固定化方式之連續流試驗 44 3-5-2 不同碳氮比之連續流試驗 44 3-5-3 不同溶氧之連續流試驗 45 3-6 固定化菌株之追蹤鑑定試驗 45 3-7 實驗設備及水質分析 55 3-7-1 實驗設備 55 3-7-2 水質分析 56 3-7-3 氮氣(nitrogen gas)分析 58 第四章 結果與討論 60 4-1 血清瓶批次試驗 60 4-1-1 氮源為氨氮廢水之硝化脫硝反應試驗 60 4-1-1-1 不同氧氣量對懸浮菌株硝化脫硝效率之影響 61 4-1-1-2 不同氧氣量對固定化菌株硝化脫硝效率之影響 67 4-1-1-3 小結 73 4-1-2 不同基質成份對異營硝化脫硝菌硝化脫硝效率之影響 75 4-1-3 相同總氮濃度條件下不同COD濃度之影響 81 4-1-4 相同COD濃度條件下不同總氮濃度之影響 89 4-1-5 不同COD/TN對異營硝化脫硝菌除氮效率之影響 96 4-1-6 批次試驗總結 99 4-2 固定化菌株硝化脫硝反應連續流試驗 100 4-2-1 相同總氮負荷條件下不同COD負荷之連續流試驗 101 4-2-2 相同COD負荷條件下不同總氮負荷之連續流試驗 107 4-2-3 不同溶氧之連續流試驗 112 4-2-4 連續流試驗總結 116 4-3 以分子生物技術鑑定反應槽菌相 117 4-4 應用固定異營硝化脫硝菌株處理家庭污水之可行性評估 119 第五章 結論與建議 121 5-1 結論 121 5-2 建議 123 參考文獻 124 | |
dc.language.iso | zh-TW | |
dc.title | 利用固定化特殊氮細菌同時去除家庭污水中碳氮之研究 | zh_TW |
dc.title | Study on simultaneously carbon and nitrogen removing from domestic wastewater with immobilized nitrogen bacteria | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李志源,徐錠基,何俊明 | |
dc.subject.keyword | 家庭污水處理,氮的去除,好氧脫硝,異營硝化,單槽硝化脫硝, | zh_TW |
dc.subject.keyword | heterotrophic nitrification,SND,immobilized cells, | en |
dc.relation.page | 127 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-24 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
顯示於系所單位: | 環境工程學研究所 |
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