請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32861
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王金和(Ching-Ho Wang) | |
dc.contributor.author | Yi-Cheng Chen | en |
dc.contributor.author | 陳奕成 | zh_TW |
dc.date.accessioned | 2021-06-13T04:17:26Z | - |
dc.date.available | 2006-07-27 | |
dc.date.copyright | 2006-07-27 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-25 | |
dc.identifier.citation | 李敏旭。應用反轉錄聚合酶反應診斷家禽流行性感冒病毒及區分血清亞型。國立中興大學碩士論文,台中,1999。
吳靜如。應用大腸桿菌表現家禽流行性感冒病毒血球凝集素以區分病毒血清亞型。國立中興大學碩士論文,台中,2001。 邱鴻文。建立以競爭型酵素連結免疫吸附法快速診斷H5亞型高病原性家禽流行性感冒之技術。國立中興大學碩士論文,台中,2002。 林仕鈺。1998-2003年台灣家禽流行性感冒監測。行政院農委會家畜衛生試驗所禽流感資訊,2004。 許捷甯。台灣家禽流行性感冒病毒H6N1亞型病原性與基因序列的關聯及垂直傳播試驗。國立台灣大學碩士論文,台北,2004。 陳志豪、謝快樂。台灣地區家禽流行性感冒之研究 (II) 病原性及病理學之研究。台灣省畜牧獸醫學會會報,54:93-106,1989。 黃元品、王金和。屠宰場色雞血清之家禽流行性感冒流行病學調查分析。台灣獸醫學雜誌,32:24-28,2006。 傅琳芳。以合成胜肽ELISA及阻斷型ELISA快速診斷家禽流行性感冒病毒H5及H7亞型抗體。國立中興大學碩士論文,台中,2004。 鄭明珠、李敏旭、郭舒亭、丁履紉、蕭終融、祁偉廉、姚中慧、林士鈺。野鳥家禽流行性感冒帶毒監測。家畜衛生試驗所研究報告,36:47-52,2000。 鄭明珠。家禽流行性感冒血清學鑑定。畜牧半月刊,60:30-33,1998。 謝快樂、黃文正、沈瑞鴻、邱新育、李龍湖、呂榮修。台灣地區家禽流行性感冒之研究 (III) 雞群病毒株之分離同定與病原性試驗。台灣省畜牧獸醫學會會報,59:45-55,1992。 Alexander DJ. Avian influenza:recent developments. Vet Bull 52:341-359, 1982. Alexander DJ. A review of avian influenza in different bird species. Vet Microbiol 74:3-13, 2000. Alexander DJ. Avian influenza-historical aspects. Proc 2nd Int Symp, Avian influenza, Athens, Georgia, USA:4-13, 1986. Alexander DJ. Avian influenza viruses and human health. Dev Biol 124:77-84, 2006. Allan WH. Diagnostic procedures-response. Proc 1st Int Symp, Avian Influenza, Beltville, Maryland, USA:167-171, 1981. Allan WH, Alexander DJ, Pomeroy BS, Parsons G. Use of virulence index tests for avian influenza viruses. Avian Dis 21:359-362, 1977. Alonso A, Darsie GC, Teixeira AC, Reis JL, Mesquita JA. Application of monoclonal antibodies to quality control of foot-and-mouth disease vaccines. Vaccine 12:682-686, 1994. Austin FJ, Webster RG. Antigenic mapping of an avian H1 influenza virus hemagglutinin and interrelationships of H1 viruses from human, pigs and birds. J Gen Virol 68:983-992, 1986. Banks J, Speidel E, Alexander DJ. Characterization of an avian influenza A virus isolated from a human is an intermediate host necessary for the emergence of pandemic influenza viruses? Arch Virol 143:781-787, 1998. Beard CW. Avian influenza antibody detection by immunodiffusion. Avian Dis 14:337-341, 1969. Beare AS. Basic and applied influenza research. CPC press. 1982. Braun S, Hepp F, Kentenich CR, Janni W, Pantel K, Riethmuller G, Willgeroth F, Sommer HL. Monoclonal antibody therapy with edrecolomab in breast cancer patients: monitoring of elimination of disseminated cytokeratin-positive tumor cells in bone marrow. Clin Cancer Res 5:3999-4004, 1999. Brown EG. Influenza virus genetics. Biomed Pharmacother 54:196-209, 2000. Brown IH, Harris PA, McCauley JW, Alexander DJ. Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in the emergence of an H1N2 virus of novel genotype. J Gen Virol 79:2947-2955, 1998. Capua I, Marangon S, Selli L, Alexander DJ, Swayne DE, Pozza MD, Parenti E, Cancellotti FM. Outbreaks of highly pathogenic avian influenza (H5N1) in Italy during October 1997 to January 1998. Avian Pathol 28:455-460, 1999. Capua I, Mutinelli F, Hablovarid MH. Avian embryo susceptibility to Italian H7N1 avian influenza viruses belonging to different genetic lineages. Arch Virol 147:1611-1621, 2002. Chang TY. Avian influenza in Taipei China. Disease information 17:118, 2004. Choi KS, Nah JJ, Choi CU, Ko YJ, Sohn HJ, Lobeau G, Kang SY, Joo YS. Monoclonal antibody-based competitive ELISA for simultaneous detection of ruminants virus antibodies. Vet Microbiol 96:1-16, 2003. Christ O, Seiter S, Matzku S, Burger C, Zoller M. Efficacy of local versus systemic application of antibody-cytokine fusion proteins in tumor therapy. Clin Cancer Res 7: 985-998, 2001. Class ECJ, Osterhaus ADME, Beek RV, Jong JCD, Rimmelzwaan GFR, Senne DA, Krauss S, Shortridge KF, Webster RG. Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus. Lancet 351:472-477, 1998. Cooper LA, Subbarao K. A simple restriction fragment length polymorphism-based strategy that can distinguish the internal genes of human H1N1, H3N2, and H5N1 influenza A viruses. J Clin Microbiol 38:2579-2583, 2000. Crowther JR. Systems in ELISA. In: Walker JM, ed. Methods in molecular biology vol.149, The ELISA guidebook. Humana Press. Totowa, New Jersey. 9- 44, 2001. Davis WC. Monoclonal Antibody Protocols. In vivo and in vitro production of monoclonal antibody. 17:169-176, 1995. Davison S, Ziegler AF, Eckroade RJ. Comparison of an antigen-capture enzyme immunoassay with virus isolation for avian influenza from field samples. Avian Dis 42:791-795, 1998. De St Groth DR, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J Immuno Meth 35:1-21, 1980. Easterday BC, Hinshaw VS, Halvorson DA. Influenza. In: Calnek BW, Barnes HJ, Beard CW, McDougald LR and Saif YM(ed.). Diseases of poultry, 10th ed. Iowa State University Press, Ames, Iowa, USA. 583-605, 1997. Ehrlich PA, Moyle WR, Moustafa ZA. Further characterization of cooperative interactions of monoclonal antibodies. J Immuno Methods 131:1906-1912, 1983. Englund L, Hard af Segerstad C. Two avian H10 influenza A virus strains with different pathogenicity for mink (Mustela vison). Arch Virol 143:653-666, 1998. Fichtnet GJ. The Pennsylvania/Virginia experience in eradication of avian influenza (H5N2). In Proceedings of the Second International Symposium on Avian Influenza. United States Animal Health Association, Athens, GA. 33-38, 1987. Fouchier RAM, Osterhaus ADME, Brown IH. Animal influenza virus surveillance. Vaccine 21:1754-1757, 2003. Fouchier RA, Munster V, Wallensten A, Bestebroer TM, Herfst S, Smith D, Rimmelzwaan GF, Olsen B, Osterhaus AD. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. J Virol 79:2814-2822, 2005. Geisler B, Seidel W, Herrmann B, Dohner L. Differences of nucleoprotein of human and avian influenza A virus strains shown by polyacrylamide gel electrophresis and by the peptide mapping technique. Arch Virol 90:289-299, 1986. Gibbs JS, Malide D, Hornung F, Bennink JR, Yewdell JW. The influenza A virus PB1-F2 protein targets the inner mitochondria membrane via a predicted basic amphipathic helix that disrupts mitochondria function. J Virol 77:7214-7224, 2003. Gould DH, Voss JL, Miller MW, Bachand AM, Cummings BA, Frank AA. Survey of cattle in northeast Colorado for evidence of chronic wasting disease: geographical and high-risk targeted sample. J Vet Diagn Invest 15:274-277, 2003. Guan Y, Shortridge KF, Krauss S, Chin PS, Dyrting KC, Ellis TM, Webster RG, Peiris M. H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China. J Virol 74:9372-9380, 2000. Guan Y, Shortridge KF, Krauss S, Li PH, Kawaoka Y, Webster RG. Emergence of avian H1N1 influenza viruses in pigs in china. J Virol 70:8041-8046, 1996. Guan Y, Shortridge KF, Krauss S, Webster RG. Molecular characterization of H9N2 influenza viruses: were they the donors of the “internal” genes of H5N1 viruses on Hong Kong? Proc Natl Acad Sci USA 96:9363-9367, 1999. Gubareva LV, Varich NL, Markushin SG, Kaverin NV. Studies on the regulation of influenza virus RNA replication: a differential inhibition of the synthesis of vRNA segments in shift-up experiments with ts mutants. Arch Virol 121:9-17, 1991. Guo YJ, Jin FG, Wang P, Wang M, Zhu JM. Isolation of influenza C virus from pigs and experimental infection of pigs with influenza C virus. J Gen Virol 64:177-182, 1983. Gut M, Jacobs L, Tyborowska J, Szewczyk B, Bienokowska-Szeqczyk K. A highly specific and sensitive competitive enzyme-linked immunosorbent assay (ELISA) based on baculovirus expressed pseudorabies virus glycoprotein gE and gI complex. Vet Microbiol 69:239-249, 1999. Halvorson D.A, Kelleher CJ, Newman JA. Avian influenza in caged laying chickens. Avian Dis 24:288-294, 1980. Halvorson D.A, Kelleher CJ, Senne DA. Epizootiology of avian influenza: effect of season on incidence in sentinel ducks and domestic turkeys in Minnesota. Appl Environ Microbiol 49:914-919, 1985. Harlow E, Lane D. Monoclonal antibodies. In Antibodies A Laboratory Manual. 1st ed., Cold spring Harbor Laboratory Printed, USA 139-244,1988. Herrmann LM, Cheever WP, Marshall KL, McGuire TC, Hutton MM, Lewis GS, Knowles DP. Detection of serum antibodies to ovine progressive pneumonia virus in sheep by using a caprine arthritis-encephalitis virus competitive-inhibition enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol 10:862-865, 2003. Hinshaw VS, Webster RG. Characterization of a new avian influenza virus subtype and proposed designation of this haemagglutinin as Hav 10. J Gen Virol 45:751-754, 1979. Hofstad MS, Barnes HJ, Calnek BW, Reid WM, Yoder HW. Avian influenza. Enghth etifion of poultry. 482-495, 1984. Horimoto T, Kawaoka Y. Direct reverse transcriptase PCR to determine virulence potential of influenza A viruses in birds. J Clin Microbiol 33:738-752, 1995. Huang RT, Rott R, Klenk HD. Influenza viruses cause hemolysis and fusion of cells. Virology 110:243-247, 1981. Ito T, Couceiro JN, Kelm S, Baum LG, Krauss S, Castrucci MR, Donatelli I, Kida H, Paulson JC, Webster RG, Kawaoka Y. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J Virol 72:7367-7373, 1998. Ito T, Kida H, Yanagawa R. Antigenic analysis of H4 influenza isolates using monoclonal antibodies to defined antigenic sites on the hemagglutinin of A/Budgerigar/Hokkaido/1/77 strain. Arch Virol 84:251-159, 1985. Johnson DC, Maxfield BG. An occurrence of avian influenza virus infection in laying chickens. Avian Dis 20:422-424, 1976. Karsten U, Stolley P, Walther I, Papsdorf G, Weber S, Conrad K, Pasternak L, Kopp J. Direct comparison of electric field-mediated and PEG-mediated cell fusion for the generation of antibody producing hybridomas. Hybridoma 7:627-633, 1988. Karunakaran D, Halvorson DA, Sivanandan V, Newman JA. Pathogenicity of avian influenza viruses isolated from wild mallard ducks and domestic turkeys. Avian Dis 32:319-323, 1988. Kawaoka Y, Yamnilova S, Chambers TM, Lvov DK, Webster RG. Molecular characterization of a new hemagglutinin, subtype H14, of influenza A virus. Virology 179:759-767, 1990. Kida H, Ito T, Yasuda J, Shimizu Y, Itakura C, Shortridge KF, Kawaoka Y, Webster RG. Potential for transmission of avian influenza viruses to pigs. J Gen Virol 75:2183-2188, 1994. Kida H, Kawaoka Y, Naeve CW, Webster RG. Antigenic and genetic conservation of H3 influenza virus in wild ducks. Virology 159:109-119, 1987. Kilbourne ED. Influenza. Plenum medical book company. New York and London. 1987. Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495-497, 1975. Kuszewski K, Brydak L. The epidemiology and history of influenza. Biomed Pharmacother 54:188-195, 2000. Lamb RA, Krug RM. Orthomyxoviruiae: The viruses and their replication. In: D.M. Kinpe, et al. Fundamental Virology, 4th ed. Lippin-Raven Publisher, Philadelphia, PA. 725-769, 2001. Lu YS, Sugimura T, Shieh HK, Lee YL, Jong MH. Isolation and identification of an influenza A virus in duck in Taiwan. Provincial Research Institue for Animal Health 21:91-104, 1985. Mcgeoch DJ, Davison AJ, Neil JC. Review Article-Some highlights of animal virus research in 1987. J Gen Virol 69:2419-2440, 1988. Moyle WR, Alderson DM, Ehrilich PA. A circular antibody-antigen complex is responsible for increased affinity shown by mixtures of monoclonal antibodies to human chorionic gonadotropin. J Immunol 131:1900-1905, 1983. Munch M, Nielsen LP, Handberg KL, Jorgensen PH. Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR-ELISA. Arch Virol 146:87-97, 2001. Murphy BR, Webster RG. Orthomyxoviruses. In: Fields BN, et al. (ed).Virology, 3rd ed. Raven Press, New York, 1397-1445, 1996. Nagata S, Yamamoto K, Ueno Y, Kurata T, Chiba J. Preferential generation of monoclonal IgG-producing hybridomas by use of vesicular stomatitis virus-mediated cell fusion. Hybridoma 10:369-378, 1991. Nelson PN, Reynold GM, Waldron EE, Ward E, Giannopoulos K, Murray PG. Monoclonal antibodies. Mol Pathol 53:111-117, 2000. Nicholson KG, Wood JM, Zambon M. Influenza. Lancet 362: 1733-1745, 2003. Ohkohchi N, Itagaki H, Doi H, Taguchi Y, Satomi S, Satoh S. New technique for producing hybridoma by laser radiation. Lasers Surg Med 27:262-268, 2000. O’neill RE, Talon J, Palese P. The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins. EMBO J 17:288-296, 1998. Osterhaus ADME, Rimmelzwaan GF, Martina BEE, Besterbroer TM, Fouchier RAM. Influenza B virus in seals. Science 288:1051-1053, 2000. Patterson S, Gross J, Oxford JS. The intracellular distribution of influenza virus matrix protein and nucleoprotein in infected cells and their relationship to haemagglutinin in the plasma membrane. J Gen Virol 69:1859-1872, 1988. Peiris M, Yuen KY, Leung CW, Chan KH, Ip PLS, Lai RWM, Orr WK, Shortridge KF. Human infection with influenza H9N2. Lancet 354:916-917, 1999. Perkins S, Zimmermann U, Foung SK. Parameters to enhance human hybridoma formation with hypoosmolar electrofusion. Hum Antibodies Hybridomas 2:155-159, 1991. Pinto LH, Holsinger LJ, Lamb RA. Influenza virus M2 protein has ion channel activity. Cell 69:517-528, 1992. Renukaradhya GJ, Suresh KB, Rajasekhar M, Shaila MS. Competitive enzyme-linked immunosorbent assay based on monoclonal antibody and recombinant hemagglutinin for serosurveillance of rinderpest virus. J Clin Microbiol 41:943-947, 2003. Rogers GN, Paulson JC, Daniels RS, Skehel JJ, Wilson IA, Wiley DC. Single amino acid substitution in influenza haemagglutinin change receptor binding specificity. Nature 304:76-78, 1983. Rohm C, Zhou N, Suss J, Mackenzie J, Webster RG. Characterization of a novel influenza hemagglutinin, H15:criteria for determination of influenza A subtypes. Virology 217:508-516, 1996. Rott R. The pathogenic determinant of influenza virus. Vet Microbiol 34:398, 1993. Samadieh B, Bankowski RA. Effect of avian influenza A viruses upon egg production and fertility of turkeys. Avian Dis 14:715-722, 1970. Sala G, Cordioli P, Moreno-Martin A, Tollis M, Brocchi E, Piccirillo A, Lavazza A. ELISA test for the detection of influenza H7 antibodies in avian sera. Avian Dis 47:1057-1059, 2003. Schafer W. Vergleichende sero-immunologische Untersuchungen uber die viren der influenza and klassichen Gefuegelpest Z Naturforsch 10:81-91, 1955. Scholtissek C, Ludwig S, Fitch WM. Analysis of influenza A virus nucleoproteins for the assessment of molecular genetic mechanism leading to new phylogenetic virus lineage. Arch Virol 131:237-250, 1993. Scholtissek C. Molecular evolution of influenza viruses. Virus Genes 11:209-215, 1996. Schultz-Cherry S, Dybdahl-Sissoko N, McGregor M, Hinshaw VS. Mink lung epithelial cells: unique cell line that supports influenza A and B virus replication. J Clin Microbiol 36:3718-3720, 1998. Schweiger B, Lange I, Heckler R, Willers H, Schreier E. Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay. Arch Virol 139:439-444, 1994. Shafer AL, Katz JB, Ernisse KA. Development and validation of a competitive enzyme-linked immunosorbent assay for detection of type A influenza antibodies in avian sera. Avian Dis 42:28-34, 1998. Shirahata S, Katakura Y, Teruya K. Cell hybridization, hybridomas, and human hybridomas. Methods Cell Biol 57:111-145, 1998. Shortridge KF, Zhou NN, Guan Y, Gao P, Ito T, Kawaoka Y, Kodihalli S, Krauss S, Markwell D, Murti KG, Norwood M, Senne D, Sims L, Takada A, Webster RG. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology 252:331-342, 1998. Singh RP, Sreenivasa BP, Dhar P, Shah LC, Bandyopadhyay SK. Development of a monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petitis ruminants (PPR) virus. Vet Microbiol 98:3-15, 2004. Sominina AA, Lisok TP, Rumel NB. Improved methods of influenza virus propagation. II. Characteristics of cell culture and allantoic virus preparations. Acta Virol 21:241-245, 1977. Spackman E, Senne DA, Myers TJ, Bulaga LL, Garber LP, Perdue ML, Lohman K, Daum LT, Suarez DL. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol 40:3256-3260, 2002. Stallknecht DE, Kearney MT, Shane SM, Zwank PJ. Effect of pH, temperature, and salinity on persistence of avian influenza viruses in water. Avian Dis 34:412-418, 1990. Steenbakkers PG, van Meel FC, Olijve W. A new approach to the generation of human or murine antibody producing hybridomas. J Immunol Methods 152:69-77, 1992. Steuler H, Rohde W, Soholtissek C. Sequence of neuramindase gene of an avian influenza A virus (A/parrot/ulster/73, H7N1). Virology 135:118-124, 1984. Suarez DL. Evolution of avian influenza viruses. Vet Microbiol 74:15-27, 2000. Subbarao K, Kimo A, Katz J, Regnery H, Lim W, Hall H, Perdue M, Swayne D, Bender C, Hung J, Hemphill M, Rowe T, Shaw M, Xu X, Fukuda K, Cox N. Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science 279:393-396, 1998. Suss J, Schafer J, Sinnecker H, Webster RG. Influenza virus subtypes in aquatic birds of eastern Germany. Arch Virol 135:101-114, 1994. Taylor HP, Armstrong SZJ, Dimmock NJ. Quantitative relationships between an influenza virus and neutralizing antibody. Virology 159:288-298, 1987. Turek R, Gresikova M, Tumova B. Isolation of influenza A virus and paramyxoviruses from sentinel domestic ducks. Acta Virol 28:156-158, 1984. Turpin EA, Lauer DC, Swayne DE. Development and evolution of a blocking enzyme-linked immunosorbent assay for detection of avian metapneumovirus type C-specific antibodies in multiple domestic avian species. J Clin Microbiol 41:3579-3583, 2003. Vey M, Orlich M, Adler S, Klenk HD, Rott R, Garten W. Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R. Virology 188:408-413, 1992. Walker JA, Kawaoka Y. Importance of conserved amino acids at the cleavage site of the hemagglutinin of a virulent avian influenza A virus. J Gen Virol 74:311-314,1993. Webster RG, Bean WJ, Gorman OT, Chamber TM, Kawaoka Y. Evolution and ecology of influenza A viruses. Microbiol Rev 56:152-179, 1992. Webster RG, Compbell CH. An inhibition test for identifying the neuraminidase antigen on influenza viruses. Avian Dis 16:1057-1066, 1972. Webster RG, Rott R. Influenza virus A pathogenicity: the pivotal role of hemagglutinin. Cell 50:665-666, 1987. Webster RG, Walker EJ. Influenza. American Sci 71:122-129, 2003. Weir E, Wong T, Gemmill I. Avian influenza outbreak: update. C`MAJ 170:785-786, 2004. Weis W, Brown JH, Cusack S, Paulson JC, Skehel JJ, Wiley DC. Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid. Nature 333:426-431, 1988. Wiley DC, Skehel JJ. The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annu Rev Biochem 56:365-394, 1987. Wojchowski DM, Sytkowski AJ. Hybridoma production by simplified avidin- mediated electrofusion. J Immuno Methods 90:173-177, 1986. Wood GW, McCauley JW, Bashiruddin JB, Alexander DJ. Deduced amino acid sequences at the hemagglutinin cleavage site of avian influenza A viruses of H5 and H7 subtypes. Arch Virol 130:209-217, 1993. Yuen KY, Chan PK, Peiris M, Tsang DNC, Que TL, Shortridge KF, Cheung PT, To WK, Ho ETF, Sung R, Cheng AF. Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet 351:467-471, 1998. Zambon MC. Epidemiology and pathogenesis of influenza. J Antimicrob Chemother. 44:3-9, 1999. Zambon MC. The pathogenesis of influenza in humans. Rev Med Virol 11:227-241, 2001. Zhironv OP. Isolation of matrix protein M1 from influenza viruses by acid-dependent extraction with nonionic detergent. Virology 186:324-330, 1992. Zhou EM, Chan M, Heckert RA, Riva J, Cantin MF. Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis 42:517-522, 1998. Zhou NN, He S, Zhang T, Zou W, Shu L, Sharp GB, Webster RG. Influenza infection in humans and pigs in southeastern China. Arch Virol 141:649-661, 1996. Zhou NN, Shortridge KF, Claas ECJ, Krauss SL, Webster RG. Rapid evolution of H5N1 influenza viruses in chickens in Hong Kong. J Virol 73:3366-3374, 1999. Ziegler AF, Darison S, Acland H, Eckroade RJ. Characteristics of H7N2 (nonpathogenic) avian influenza virus infections in commercial layers, in Pennsylvenia, 1997-1998. Avian Dis 43:142-149, 1998. Ziegler T, Hall H, Sanchez-Fauquier A, Gamble WC, Cox NJ. Type- and subtype- specific detection of influenza viruses in clinical specimen by rapid culture assay. J Clin Microbiol 33:318-312, 1995. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32861 | - |
dc.description.abstract | 本研究目的以sandwich ELISA快速診斷H5亞型抗原,以blocking ELISA快速診斷H5亞型抗體。其方法為將3233/04之濃縮純化病毒與其HA重組蛋白免疫小鼠進行單源抗體的製備,並將製備好的單源抗體以免疫墨點法 (immunodot blot assay)、西方轉漬法 (western blot assay) 及血球凝集抑制試驗 (hemagglutination inhibition test) 分析其特性,接著進行單源抗體純化與過氧化氫酶標示後成為追蹤子 (tracer),最後以blocking ELISA的架構進行野外H5亞型陽性血清檢測,以sandwich ELISA的架構進行H5亞型病毒檢測。在sandwich ELISA結果方面,只能偵測到3233/04 (H5N2) 病毒株,且病毒力價於106 EID50/0.1 mL以上時皆可被檢測到。在blocking ELISA結果方面,以228個H5陰性的血清樣本進行檢測,其特異性為97.36% (222/228);以234個H5陽性的血清樣本進行檢測,其敏感性為98.29% (230/234)。
家禽流行性感冒病毒是否會經由母雞垂直傳染給小雞的證據目前仍缺乏,因此本實驗為將宜蘭雞場分離到的H6N1亞型病毒株2838K/00,一方面進行母雞攻毒試驗,視其病毒是否會經由母雞垂直傳染給小雞,而另一方面則進行無特定病原雞胚胎蛋攻毒試驗,視其小雞是否會將病毒直接帶毒出來。其方法為取108.65 EID50/0.1 mL的病毒量,以鼻內接種法攻毒於產蛋母雞,另將病毒液稀釋成1EID50/0.1 mL的劑量,直接將病毒液經由尿囊腔接種於無特定病原的雞胚胎蛋中,接著收取攻毒後母雞所生小雞與直接攻毒於SPF雞胚胎蛋出生小雞之氣管、腎臟與卵黃囊,以SPF雞胚胎蛋分離兩代後,抽取尿囊液以RT-PCR的方式進行AIV NP基因的檢測。其結果可從卵黃卵白中檢測到病毒核酸,但出生小雞檢體其病毒分離結果為陰性,依此結果推論家禽流行性感冒病毒不會有垂直傳播的情形發生。 | zh_TW |
dc.description.abstract | In this study, we used sandwich ELISA to detect H5 virus and blocking ELISA to detect H5 antibody. The concentrated purified virus (3233/04) and hemagglutinin (HA) recombinant proteins were used for the production of monoclonal antibodies. These monoclonal antibodies were confirmed to be specific to the H5 HA by immunodot blot assay, western blot assay and hemagglutination inhibition test. These monoclonal antibodies were purified and then labeled with horseradish peroxidase to become tracer. The tracer was used for the detection of H5 antibody in serum by blocking ELISA and for the H5 subtype virus by sandwich ELISA. The results showed that sandwich ELISA detected the H5 virus above the titer of 106 EID50/0.1 mL. The specificity and sensitivity for blocking ELISA were 97.36% (222/228) and 98.29% (230/234), respectively.
Little information about AIV vertical transmission is available. This study aimed to prove the existence of vertical transmission of AIV from hens to embryos. We used a H6N1 low pathogenetic AIV Taiwanese isolate (Yilan, Taiwan), 2838K/00, in the vertical transmission test. After challenging the hens with 108.65 EID50/0.1 mL of 2838K/00, and inoculating the specific-pathogen-free embryonated eggs with 1EID50/0.1 mL of 2838K/00, the embryos were hatched. The tracheas, kidneys, and yolk sacs were collected from the hatched chicks for the virus isolation by the allantoic sac route. After two passages, we harvested the allantoic fluid and tested for the detection of NP gene of AIV by RT-PCR. The results showed that we detected the nucleic acid of AIV in the yolk and albumen mixture from unfertilized eggs. But we didn’t detect the AIV in the hatched chicks. According to above results, AIV seems to be no vertical transmission in chickens. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:17:26Z (GMT). No. of bitstreams: 1 ntu-95-R93629002-1.pdf: 2705755 bytes, checksum: 0fc506cb4073f8e26f3ff83da6a5612e (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 目錄------------------------------------------------------------------------------------------------I
表次---------------------------------------------------------------------------------------------VII 圖次--------------------------------------------------------------------------------------------VIII 中文摘要-----------------------------------------------------------------------------------------X 英文摘要----------------------------------------------------------------------------------------XI 第一章 緒言-------------------------------------------------------------------------------------1 第二章 文獻回顧-------------------------------------------------------------------------------2 2-1 歷史背景------------------------------------------------------------------------------------2 2-2 病毒特徵------------------------------------------------------------------------------------5 2-3 病毒型態、結構與功能------------------------------------------------------------------6 2-3.1 血球凝集素-------------------------------------------------------------------------------6 2-3.2 神經胺酸酶-------------------------------------------------------------------------------7 2-3.3 核蛋白-------------------------------------------------------------------------------------8 2-3.4 基質蛋白----------------------------------------------------------------------------------8 2-3.5 非結構蛋白-------------------------------------------------------------------------------9 2-3.6 聚合酶蛋白-------------------------------------------------------------------------------9 2-4 病毒複製-----------------------------------------------------------------------------------10 2-5 病毒抗原變異性--------------------------------------------------------------------------11 2-6 病毒物理化學特性-----------------------------------------------------------------------11 2-7 宿主範圍-----------------------------------------------------------------------------------12 2-8 臨床症狀與病理變化--------------------------------------------------------------------13 2-9 病原性之評估-----------------------------------------------------------------------------13 2-10 病毒之命名------------------------------------------------------------------------------15 2-11 診斷方法----------------------------------------------------------------------------------15 2-11.1 偵測病毒抗原-------------------------------------------------------------------------15 2-11.2 偵測病毒抗體-------------------------------------------------------------------------17 2-12 酵素連結免疫吸附法之應用原理---------------------------------------------------19 2-13 單株抗體之歷史背景------------------------------------------------------------------20 2-13.1 細胞融合原理-------------------------------------------------------------------------20 2-13.2 細胞融合方式-------------------------------------------------------------------------20 2-13.3 HAT篩選原理-------------------------------------------------------------------------21 2-13.4 單株抗體之特性與應用-------------------------------------------------------------21 第三章 材料與方法---------------------------------------------------------------------------23 第一節 野鳥家禽流行性感冒帶毒之監測------------------------------------------------23 3-1.1 採樣時間與地點------------------------------------------------------------------------23 3-1.2 樣本數大小------------------------------------------------------------------------------23 3-1.3 病毒分離與鑑定------------------------------------------------------------------------23 3-1.3.1 樣本採集------------------------------------------------------------------------------23 3-1.3.2 樣本處理------------------------------------------------------------------------------24 3-1.3.3 病毒增殖------------------------------------------------------------------------------24 3-1.3.4 RNA之萃取---------------------------------------------------------------------------24 3-1.3.5 反轉錄聚合酶鏈反應---------------------------------------------------------------25 3-1.3.6 瓊膠醣凝膠電泳---------------------------------------------------------------------26 3-1.3.7 聚合酶鏈反應產物之純化---------------------------------------------------------27 3-1.3.8 聚合酶鏈反應產物之選殖---------------------------------------------------------27 3-1.3.9 Colony PCR---------------------------------------------------------------------------28 3-1.3.10 核酸序列之定序-------------------------------------------------------------------29 3-1.3.11 序列之分析-------------------------------------------------------------------------29 第二節 ELISA診斷試驗之開發-----------------------------------------------------------29 3-2.1 病毒來源--------------------------------------------------------------------------------29 3-2.2 屠宰場血清樣本採集-----------------------------------------------------------------30 3-2.3 血清樣本抗體檢測--------------------------------------------------------------------30 3-2.4 紅血球凝集試驗及紅血球凝集抑制試驗-----------------------------------------30 3-2.4.1 製備紅血球溶液---------------------------------------------------------------------30 3-2.4.2 紅血球凝集試驗---------------------------------------------------------------------31 3-2.4.3 紅血球凝集抑制試驗---------------------------------------------------------------31 3-2.5 病毒濃縮與純化-----------------------------------------------------------------------31 3-2.6 單株抗體--------------------------------------------------------------------------------32 3-2.6.1 單株抗體製備------------------------------------------------------------------------32 3-2.6.2 HA基因之選殖-----------------------------------------------------------------------32 3-2.6.3 建構表現載體------------------------------------------------------------------------33 3-2.6.4 免疫計畫------------------------------------------------------------------------------33 3-2.6.5 單株抗體篩選------------------------------------------------------------------------34 3-2.6.6 單株抗體純化------------------------------------------------------------------------34 3-2.6.7 蛋白質定量---------------------------------------------------------------------------34 3-2.6.8 單株抗體分析------------------------------------------------------------------------35 3-2.6.8.1 單株抗體亞型分析----------------------------------------------------------------35 3-2.6.8.2 免疫墨點法分析-------------------------------------------------------------------35 3-2.6.8.3 聚丙烯醯胺膠片電泳及西方墨點法分析-------------------------------------36 3-2.6.8.4 病毒中和試驗----------------------------------------------------------------------37 3-2.6.8.5 胜肽矩陣----------------------------------------------------------------------------38 3-2.6.9 以過氧化氫酶標示單株抗體------------------------------------------------------38 3-2.6.10 阻斷型ELISA之開發-------------------------------------------------------------38 3-2.6.10.1 阻斷型ELISA最佳化-----------------------------------------------------------38 3-2.6.10.2 單株抗體與15種禽流感病毒亞型高免疫血清之阻斷型ELISA試驗--39 3-2.6.10.3 阻斷型ELISA之敏感性與特異性分析--------------------------------------40 3-2.6.11 三明治型ELISA之開發-----------------------------------------------------------40 3-2.6.11.1 三明治型ELISA最佳化--------------------------------------------------------40 3-2.6.11.2 三明治型ELISA之敏感性與特異性分析-----------------------------------40 第三節 攻毒至SPF雞胚胎蛋探討孵出小雞之AIV帶毒情形-----------------------41 3-3.1 病毒株之挑選---------------------------------------------------------------------------41 3-3.2 病毒株之力價測定---------------------------------------------------------------------41 3-3.3 實驗設計---------------------------------------------------------------------------------41 3-3.3.1 SPF雞胚胎蛋攻毒試驗組-----------------------------------------------------------41 3-3.3.2 SPF雞胚胎蛋空白對照組-----------------------------------------------------------42 3-3.4 以SPF雞胚胎蛋繼代進行病毒增殖與鑑定---------------------------------------42 第四節 攻毒至產蛋母雞探討所生小雞之AIV帶毒情形------------------------------43 3-4.1 病毒株之挑選---------------------------------------------------------------------------43 3-4.2 病毒株之力價測定---------------------------------------------------------------------43 3-4.3 實驗動物---------------------------------------------------------------------------------43 3-4.4 飼料與飲水------------------------------------------------------------------------------43 3-4.5 飼養環境---------------------------------------------------------------------------------43 3-4.6 實驗設計---------------------------------------------------------------------------------44 3-4.7 攻毒母雞抗體監測---------------------------------------------------------------------45 第四章 結果------------------------------------------------------------------------------------46 第一節 野鳥家禽流行性感冒帶毒之監測------------------------------------------------46 4-1.1 家禽流行性感冒病毒之分離---------------------------------------------------------46 4-1.2 家禽流行性感冒病毒之鑑定---------------------------------------------------------46 4-1.3 家禽流行性感冒病毒之序列分析---------------------------------------------------46 第二節 ELISA診斷試劑之開發------------------------------------------------------------47 4-2.1 屠宰場血清樣本採集------------------------------------------------------------------47 4-2.2 血清樣本抗體檢測---------------------------------------------------------------------47 4-2.3 病毒濃縮與純化------------------------------------------------------------------------47 4-2.4 單株抗體---------------------------------------------------------------------------------47 4-2.4.1 單株抗體篩選------------------------------------------------------------------------47 4-2.4.2 蛋白質定量---------------------------------------------------------------------------47 4-2.4.3 單株抗體亞型分析------------------------------------------------------------------48 4-2.4.4 免疫墨點法---------------------------------------------------------------------------48 4-2.4.5 SDS-PAGE及Western blot assay--------------------------------------------------48 4-2.4.6 單株抗體HI分析--------------------------------------------------------------------49 4-2.4.7 病毒中和試驗------------------------------------------------------------------------49 4-2.4.8 胜肽矩陣------------------------------------------------------------------------------49 4-2.4.9 以過氧化氫酶標示單株抗體------------------------------------------------------49 4-2.4.10 阻斷型ELISA之開發--------------------------------------------------------------50 4-2.4.10.1 阻斷型ELISA最佳化-----------------------------------------------------------50 4-2.4.10.2 單株抗體與15種禽流感病毒亞型高免疫血清之阻斷型ELISA試驗--50 4-2.4.10.3 阻斷型ELISA之敏感性與特異性分析--------------------------------------50 4-2.4.11 三明治型ELISA之開發----------------------------------------------------------51 4-2.4.11.1 三明治型ELISA最佳化--------------------------------------------------------51 4-2.4.11.2 三明治型ELISA之特異性與敏感性分析-----------------------------------52 第三節 攻毒至SPF雞胚胎蛋探討孵出小雞之AIV帶毒情形------------------------52 4-3.1 病毒株之力價測定---------------------------------------------------------------------52 4-3.2 SPF雞胚胎蛋攻毒試驗組結果-------------------------------------------------------52 4-3.3 SPF雞胚胎蛋空白對照組-------------------------------------------------------------52 第四節 攻毒至產蛋母雞探討所生小雞之AIV帶毒情形------------------------------53 4-4.1 母雞產蛋之檢測結果------------------------------------------------------------------53 4-4.2 母雞抗體檢測之結果------------------------------------------------------------------53 第五章 討論與結論---------------------------------------------------------------------------54 第六章 參考文獻------------------------------------------------------------------------------61 | |
dc.language.iso | zh-TW | |
dc.title | 家禽流行性感冒病毒H5亞型ELISA診斷試劑之開發及垂直傳播試驗 | zh_TW |
dc.title | Development of ELISA Kit for Detecting H5 Subtype Avian Influenza Viruses and Vertical Transmission Test | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林茂勇,蔡向榮,連一洋 | |
dc.subject.keyword | 家禽流行性感冒病毒,酵素連結免疫吸附法, | zh_TW |
dc.subject.keyword | Avian influenza virus,ELISA, | en |
dc.relation.page | 107 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-25 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 2.64 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。