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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 林文澧(Win-Li Lin) | |
dc.contributor.author | Li-Wei Sung | en |
dc.contributor.author | 宋立偉 | zh_TW |
dc.date.accessioned | 2021-06-13T04:15:14Z | - |
dc.date.available | 2008-07-28 | |
dc.date.copyright | 2006-07-28 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-25 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32772 | - |
dc.description.abstract | 震波已用於治療骨骼肌肉疾病,但是對於其機制尚無法確定。目前有多篇論文指出其主要治癒機制與該區域的血液供給有關。本研究主要之目的在探討,肌腱炎模型(tendinopathy model)經過震波治療後,肌腱內之血管情形有何改變。實驗分別針對兩種不同型的肌腱炎做探討:慢性肌腱炎(tendinosis)、急性肌腱炎(tendonitis)。
治療慢性肌腱炎的目標,主要是想要改善肌腱部份缺血、或是血流不足的情形,以正常肌腱作為實驗模型。除了震波治療(0.49mJ/mm2, 4Hz, 1000 impulses)外,亦比較使用超音波顯影劑(0.06毫升, DEFINITY® )加上震波,是否有更強的效果。在震波治療後的第28天,使用RT-PCR來分析血管內皮細胞成長因子(VEGF)有無表現,以及用病理切片的方法來統計血管是否增生。病理切片之結果顯示震波與震波-超音波顯影劑治療後28天,肌腱內血管比未治療來得多,但震波與震波-超音波顯影劑兩者並無差異。而RT-PCR的結果顯示震波治療前後VEGF的表現無變化。 急性肌腱炎模型建立是注射膠原分解酶,然後在發炎最嚴重的時期(注射後第三天)做震波治療,用能量都卜勒超音波影像紀錄血管在震波治療後之變化。且用3D影像分析血管之變化情形。實驗結果發現在震波治療後,6個實驗組中有4組,有立即(隔天)血流增加的情形(66%),其中有2組發炎時間有縮短的情形(33%)。 從兩實驗的結果顯示震波對於血流是有影響的,在慢性肌腱炎上是有新生血管的幫助。在急性肌腱炎模型中有立即增加血流,不過在此增加血流是否是有助於治療,還是會讓患者更加疼痛,這部份還有許多議題可討論。 | zh_TW |
dc.description.abstract | Shock wave therapy is a new modality and an alternative method for the promotion of bone growth and tendon repair. However, mechanisms by which shock wave therapy promotes tendon repair is not fully understood. There are several research reports indicating that its leading clinical mechanism relates to the blood supply. The purpose of this study was to investigate the effect of vessels varies after the tendon treated by shock wave. The study focuses on two different types of tendinopathy:tendinosis and tendonitis.
To impove the blood supply in tendon is the clinical purpose of tendinosis therapy. In addition to shock wave treatment (0.49mJ/mm2, 4Hz, 1000 impulses), we also combined ultrasound contrast agent (0.06ml, DEFINITY® ) to see if neovascularization could be enhanced. On the 28th day after shock wave treatment, RT-PCR method was used to analyze if VEGF expression enhanced and HE & IHC stain to calculate if number of vessels in tendon increased. The result of HE stain demonstrates that after 28 days of the shock wave treatment and shock wave contrast agent combination, the number of vessels increased compared with the untreated group. However no VEGF expression increased was found on change on the 28th day after the shock wave treatment. The model of tendonitis is induced by collagenase injection. Shock wave was given on the 3rd day after the injection and using 2-D and 3-D power Doppler sonography was used to monitor the variation of vessels after the treatment. The results show that after the shock wave treatment, there are 4 among 6 experiment groups (66%) having blood flow increase at the following day. The results also show that shock wave has effect on blood flow, increasing neo-vessels for health tendons. For acute tendonitis, blood flow enhanced. However, it was not been confirmed that whether the rise of blood flow will help the therapy or will make patients more painful. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:15:14Z (GMT). No. of bitstreams: 1 ntu-95-R93548036-1.pdf: 2508386 bytes, checksum: 0c8c5ea400f95af94f73904aec1d3b47 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 摘要 I
ABSTRACT II 目錄 IV 圖目錄 VI 表目錄 VII 第一章 緒論 1 1-1 超音波與震波的應用 1 1-2 研究動機和目的 4 第二章 基礎理論 5 2-1 肌腱與肌腱炎 5 2-1-1 肌腱(Tendon) 5 2-1-2 肌腱炎(Tendinitis) 7 2-2 VEGF 8 2-3 RT-PCR 10 2-3-1 PCR 10 2-3-2 RT 11 2-4 免疫染色 IHC 12 2-5 能量都卜勒超音波 (POWER DOPPLER ULTRASOUND) 13 第三章 實驗設備與材料 13 3-1 兔子、麻醉、安樂死 13 3-2 體外震波治療儀 13 3-3 診斷式超音波儀 13 3-3-1 Titan Sonosite 13 3-3-2 Xario Toshiba 13 3-4 超音波顯影劑(CONTRAST AGENT) 13 3-5 膠原分解酶(COLLAGENASE) 13 第四章 實驗方法 13 4-1 實驗流程示意圖 13 4-2正常肌腱模型之動物實驗 13 4-3 RT-PCR 13 4-3-1 抽取RNA 13 4-3-2 DNase;測OD值 13 4-3-3 RT 13 4-3-4 PCR 13 4-4 HE STAIN & IHC STAIN 13 4-4-1 HE stain 13 4-4-2 IHC stain 13 4-5 切片染色 & PCR結果之統計分析 13 4-6 急性肌腱炎模型之動物實驗 13 4-6-1 注射膠原分解酶(collagenase) 13 4-6-2 B-mode超音波影像、厚度變化紀錄 13 4-6-3 急性肌腱炎模型之震波照射 13 4-6-4 能量都卜勒超音波之血管分析方法 13 第五章 實驗結果 13 5-1 PCR實驗結果 13 5-1-1 Beta-actin引子之測試結果 13 5-1-2 VEGF引子之測試結果 13 5-1-3 各組PCR結果 13 5-2 切片染色之結果 13 5-2-1 HE stain統計 13 5-2-2 IHC stain統計 13 5-3 急性肌腱炎之實驗結果 13 5-3-1 注射collagenase VS. 注射生理食鹽水 13 5-3-2震波VS. 對照組 13 5-3-3 解剖、切片結果 13 5-3-4 血管之3D影像結果 13 第六章 討論 13 6-1 RT-PCR 13 6-2 病理切片 13 6-3 超音波顯影 13 6-4 急性肌腱炎MODEL實驗之結果 13 6-5 3D影像 13 第七章 結論 13 第八章 未來展望 13 參考文獻 13 | |
dc.language.iso | zh-TW | |
dc.title | 體外震波治療對於肌腱內血管之影響及其機轉探討 | zh_TW |
dc.title | The Effects and Mechanism of Shock Wave Therapy on the Vessels in Tendon | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳文翔(Wen-Shiang Chen) | |
dc.contributor.oralexamcommittee | 江惠華(Hui-Hua Chiang),葉秩光(Jr-Guang Ye) | |
dc.subject.keyword | 體外震波,血管內皮生長因子,新生血管,膠原分解酶, | zh_TW |
dc.subject.keyword | Shock waves,VEGF,Neovascularization,Collagenase, | en |
dc.relation.page | 66 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-25 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
Appears in Collections: | 醫學工程學研究所 |
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