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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 潘建源 | |
dc.contributor.author | Ming-Ling Chen | en |
dc.contributor.author | 陳明靈 | zh_TW |
dc.date.accessioned | 2021-06-13T04:12:38Z | - |
dc.date.available | 2011-07-29 | |
dc.date.copyright | 2006-07-29 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-24 | |
dc.identifier.citation | Burgoyne, R. D., and Weiss, J. L. (2001). The neuronal calcium sensor family of Ca2+-binding proteins. Biochem J 353, 1-12.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32634 | - |
dc.description.abstract | 鈣調素(calmodulin)是細胞中常見的鈣離子結合蛋白,調控細胞內許多重要的生理機制。而近幾年發現有一鈣離子結合蛋白家族,在結構上與鈣調素類似,且主要表現在神經細胞中。CaBP1因為alternative splicing,而有L-CaBP1和S-CaBP1之分。在本實驗中我們以電生理的技術,探討鈣離子結合蛋白-1 (CaBP1)在興奮性細胞中所扮演的功能。首先由大鼠E14.5胚胎大腦中選殖出CaBP1在其C端接上EYFP,再轉殖入初級培養的牛嗜鉻細胞及胎鼠大腦神經細胞中。結果顯示,在嗜鉻細胞中CaBP1主要分布在細胞膜上,且會抑制鈣離子電流及胞吐作用。而無法進行荳蔻酸化(myristoylation)修飾的G2A變異型,則主要分布在細胞質中,且無此抑制作用。而在神經細胞中,CaBP1對鈣離子電流,並無顯著的抑制作用。但當以高鉀溶液刺激這兩種細胞,鈣離子濃度上升都會因CaBP1大量表現而被抑制,但G2A變異型則無此作用。這些結果顯示,CaBP1需要在膜上才能發揮其對鈣離子通道及胞吐作用的抑制功能。 | zh_TW |
dc.description.abstract | Calmodulin is a calcium binding protein ubiquitously expressed in cells and modulates many physiological activities. Recently, a new family of calcium binding proteins was found to have similar structure to calmodulin. In this report, the functions of a member of this family, calcium binding protein-1 (CaBP1), in modulating the stimulus-secretion coupling in excitable cells was characterized. Alternative splicing L- and S-CaBP1 were cloned from rat E14.5 embryonic cortical neurons. When expressed in primary cultured bovine chromaffin and embryonic neurons, both L- and S-CaBP1-EYFP fusion proteins were found to be localized on the plasma membrane but the G2A mutants, deficient in myristoylation, was in cytosol. The changes in calcium currents and capacitance evoked by membrane depolarization were inhibited by the expression of wild type L- and S-CaBP1 but not G2A mutants. The Ca2+ imaging recorded form cells stimulated by high K+ buffer also showed comparable results. These suggest that overexpression of CaBP1s in chromaffin cells and neurons inhibit calcium currents and exocytosis. These suggest the importance of CaBP1 in modulating the stimulus-secretion coupling in excitable cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:12:38Z (GMT). No. of bitstreams: 1 ntu-95-R93b41017-1.pdf: 552077 bytes, checksum: df5aa654c5713866109691d94dcacbcb (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | Ⅰ. ABSTRACTS
Ⅰ.1 中文摘要……………………………………………………………1 Ⅰ.2 英文摘要…………………………………………………………….2 Ⅱ. INTRODUCTION Ⅱ.1胞內鈣離子調控途徑......……………………………………………….3 Ⅱ.2 鈣調素的功能……………………………………………………………4 Ⅱ.3神經鈣離子感應蛋白.........................................................................5 Ⅱ.4鈣離子結合蛋白………………………………………………................6 Ⅱ.5 我們的研究結果……………………………………………………..…..7 Ⅲ. METHODS AND MATERIALS Ⅲ.1初級細胞培養……………………………………...………....................9 Ⅲ.2轉殖………………………………………………………………………...9 Ⅲ.3電生理..............................................................................................10 Ⅲ.4螢光影像..……………………………………………………………….10 Ⅲ.5鈣離子影像……………………………………………………………...11 Ⅲ.6 PCR……………………………………………………………………...11 Ⅲ.7 Primer…………………………………………………………………....12 Ⅲ.8 Construct…………………………………………………………….…..13 Ⅲ.9 Statistical analysis…………………………………………………..….13 Ⅳ. RESULTS Ⅳ.1 CaBP1在牛腎上腺嗜鉻細胞中表現位置………………………….....14 Ⅳ.2 CaBP1在嗜鉻細胞中抑制高鉀刺激的鈣離子濃度變化……………15 Ⅳ.3 CaBP1抑制鈣離子通道………………………………………….…….15 Ⅳ.4胞吐作用的影響……………………………………………………...…..16 Ⅳ.5神經細胞中CaBP1的表現位置………………………………..……...18 Ⅳ.6 CaBP1在神經細胞中抑制鈣離子濃度上升……………………..…...18 Ⅳ.7神經細胞的鈣離子電流…...19 Ⅳ.8 CaBP1在神經細胞中的影響……………………………...……………20 Ⅴ.DISCUSSION…………………………………………………..…………...21 Ⅵ.REFERENCE……………………………………………..………………...24 Ⅶ.Figures………………………………………………..…………………….27 | |
dc.language.iso | zh-TW | |
dc.title | 鈣離子結合蛋-1對調控興奮性細胞刺激-分泌耦合機轉之研究 | zh_TW |
dc.title | Modulation of Stimulus-secretion Coupling by Calcium Binding protein-1 in Excitable Cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 閔明源,林崇智,陳建璋 | |
dc.subject.keyword | 鈣離子通道,胞吐作用,鈣離子結合蛋白1,膜片箝制, | zh_TW |
dc.subject.keyword | calcium channel,exocytosis,CaBP1,patch clamp, | en |
dc.relation.page | 35 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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