請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32603
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭石通 | |
dc.contributor.author | Yu-Chi King | en |
dc.contributor.author | 金禹圻 | zh_TW |
dc.date.accessioned | 2021-06-13T04:12:10Z | - |
dc.date.available | 2006-07-27 | |
dc.date.copyright | 2006-07-27 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-24 | |
dc.identifier.citation | 行政院農業委員會台南區農業改良場2000年年報。
國立台灣大學生命科學系普通植物學指導,2004。 王瑞章、孫文章、陳俊仁、胡文若 (2005). 外銷文心蘭產銷班輔導概況。台南區農業專訊 51, 20-22. 周以祥 (2005). 甘藷中SUMO基因表現及其訊息傳遞路徑。 國立台灣大學植物科學研究所碩士論文。 徐偉恩 (2004). 文心蘭切花老化相關基因之篩選。 國立台灣大學植物科學研究所碩士論文。 黃肇家 (1998). 中華農業研究 47, 125-134. Adams, N.D., and Yang, S.F. (1979). Ethylene biosynthesis: indentification of 1-aminocyclopropane- 1-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc. Natl. Acad. Sci. USA 76, 70-174. Bastow, R., Mylne, J.S., Lister, C., Lippman, Z., Martienssen, R.A., and Dean, C. (2004). Vernalization requires epigenetic silencing of FLC by histone methylation. Nature 427, 164-167. Borden, K.L. (2000). RING domains: master builders of molecular scaffolds? J. Mol. Biol. 295, 1103-1112. Dijk, K., Marley, K.E., Jeong, B., Xu, J., Hesson, J., Cerny, R.L., Waterborg, J.H., and Ceruttic, H. (2005). Monomethyl Histone H3 Lysine 4 as an Epigenetic Mark for Silenced Euchromatin in Chlamydomonas. Plant cell 17, 2439-2453. Fang, S.C., and Fernandez, D.E. (2002). Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation. Plant Physiol. 130, 78-89. Freemont, P.S., Hanson, I.M., and Trowsdale, J. (1991). A novel cysteine-rich sequence motif. Cell 64, 483-484. Gagne, J.M., Downes, B.P., Shiu, S.H., Durski, A.M., and Vierstra, R.D. (2002). The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis. Proc. Natl. Acad. Sci. USA 99, 11519-11524. Gan, S., and Amasino, R.M. (1997). Making sense of senescence: molecular gene regulation and manipulation of leaf senescence. Plant Physiol. 133, 313-319. Glickman, M.H., and Ciechanover, A. (2002). The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol. Rev. 82, 373-428. He, Y., and Amasino, R.M. (2005). Role of chromatin modification in flowering-time control. Trends in Plant Sci. 10, 30-35. He, Y., and Gan, S. (2002). A gene encoding an acyl hydrolase is involved in leaf senescence in Arabidopsis. Plant Cell 14, 805-815. Hensel, L.L., Grbic, V., Baumgarten, D.A., and Bleecker, A.D. (1993). Developmental and age-regulated processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell 5, 553-564. Henskens, J., Rouwendal, G., Have, A., and Woltering, E. (1994). Molecular cloning of two different ACC synthase PCR fragments in carnation flowers and organ-specific expression of the corresponding genes. Plant Mol. Biol. 5, 1-6. Hinderhofer, K., and Zentgraf, U. (2001). Identification of a transcription factor specifically expressed at the onset of leaf senescence. Planta 213, 469-473. Hwang, W.W., Venkatasubrahmanyam, S., Ianculescu, A.G., Tong, A., Boone, C., and Madhani, H.D. (2003). A Conserved RING Finger Protein Required for Histone H2B Monoubiquitination and Cell Size Control. Mol. Cell 11, 261-266. Lohman, K.N., Gan, S., John, M.C., and Amasino, R.M. (1994). Molecular analysis of natural leaf senescence in Arabidopsis thaliana. Physiol. Plant 92, 322-328. Lovering, R., Hanson, I.M., Borden, K.L., Martin, S., O’Reilly, N.J., Evan, G.I., Rahman, D., Pappin, D.J., Trowsdale, J., and Freemont, P.S. (1993). Identification and preliminary characterization of a protein motif related to the zinc finger. Proc. Natl. Acad. Sci. USA 90, 2112-2116. Matile, P., and Winkbach, F. (1971). Function of lysosomes and lysosomal enzymes in the senescencing corolla of the morning glory. J. Expt. Bot. 22, 759-771. Mayak, S., Borochov, A., and Triosh, T. (1985). Transient water stress in carnation flowers: effect of 1-aminocyclopropane- 1-carboxylic acid. J. Expt. Bot. 36, 800-806. Moehs, C.P., McElwain, E.F., and Spiker, S. (1988). Chromosomal proteins of Arabidopsis thaliana. Plant Mol. Biol. 11, 507-515. Olszewski, N., Sun, T.P., and Gubler, F. (2002). Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell Supplement s61-s80. Osley, M.A. (2004). H2B ubiquitylation: the end is in sight. Biochimica et Biophysica Acta 1677, 74-78. Risseeuw, E.P., Daskalchuk, T.E., Banks, T.W., Liu, E., Cotelesage, J., Hellmann, H., Estelle, M., Somers, D.E., and Crosby, W.L. (2003). Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis. Plant J. 34, 753-767. Robzyk, K., Recht, J., and Osley, M.A. (2000). Rad6-dependent ubiquitination of histone H2B in yeast. Science 287, 501-504. Schnell, J.D., and Hicke, L. (2003). Non-traditional functions of ubiquitin and ubiquitin-binding proteins. J. Biol. Chem. 278, 35857-35860. Smalle, J., and Vierstra, R.D. (2004). The ubiquitin 26s proteasome proteolytic pathway. Annu. Rev. Plant Biol. 55, 555-590. Stone, S.L., Hauksdo´ttir, H., Troy, A., Herschleb, J., Kraft, E., and Callis, J. (2005). Functional analysis of the RING-Type ubiquitin ligase family of Arabidopsis. Plant Physiol. 137, 13-30. Sugita, M., Yoshida, K., and Sasaki, K. (1979). Germination-induced changes in chromosomal proteins of spring and winter wheat embryos. Plant Physiol. 64, 780-785. Thompson, J.E., Froese, C.D., Madey, E., Smith, M.D., and Hong, Y. (1998). Lipid metabolism during plant senescence. Prog. Lipid Res. 37, 119-141. Vierstra, R.D. (2003). The ubiquitin/26S proteasome pathway, the complex last chapter in the life of many plant proteins. Trends in Plant Sci. 8, 135-142. Watada, A.E., Herner, R.C., Kader, A.A., Romani, R.J., and Staby, G.L. (1984). Terminology for the description of developmental stages of horticulture crops. Hort. Sci. 19, 20-21. Wood, A., Krogan, N.J., Dover, J., Schneider, J., Heidt, J., Boateng, M.A., Dean, K., Golshani, A., Zhang, Y., Greenblatt, J.F., Johnston, M., and Shilatifard1, A. (2003a). Bre1, an E3 ubiquitin ligase required for ecruitment and substrate selection of Rad6 at a promoter. Mol. cell 11, 267-274. Wood, A., Schneider, J., Dover, J., Johnston, M., and Shilatifard, A. (2003b). The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. J. of Biol. Chem. 278, 34739-34742. Xiao, T., Kao, C.F., Krogan, N.J., Sun, Z.W., Greenblatt, J.F., Osley, M.A., and Strahl1, B.D. (2005). Histone H2B ubiquitylation is associated with elongating RNA Polymerase II. Mol. and Cellular Biol. 25, 637-651. Yamagami, T., Tsuchisaka, A., Yamada, K., William F. Haddon, W.F., Harden,L.A., and Theologis, A. (2003). Biochemical diversity among the 1-amino-cyclopropane-1-carboxylate synthase isozymes encoded by the Arabidopsis gene family. J. Biol. Chem. 278, 49102-49112. Yang, S.F., and Hoffman, N.E. (1984). Ethylene biosynthesis and its regulation in higher plants. Annu. Rev. Plant Physiol. 35, 155-189. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32603 | - |
dc.description.abstract | 文心蘭外銷可分為空運與海運,但由於現階段設備不足,切花空運品質不易維持,且空運成本較高,因此目前還是以海運為主。海運需時較久,赴日銷售時的瓶插壽命不長,嚴重影響到文心蘭切花的品質,因此如何延緩文心蘭切花老化為目前文心蘭切花外銷的重要研究方向。本實驗室曾利用抑制性雜合扣除法 (Suppression Subtractive Hybridization)、蛋白質二維電泳、Differential Display等方式,釣取出一些文心蘭老化相關基因OSAGs (Oncidium Senescence-Associated Genes),大約200~300bps的片段,並利用半定量RT-PCR進行進一步的篩選。以Real-time PCR的方法再做一次確認,確定OSAG243為老化相關基因。利用RACE釣取OSAG243 cDNA全長,並將全長經由NCBI blast,比對到了阿拉伯芥的一個C3HC4-type RING finger protein (At243)。
將OSAG243大量表現於阿拉伯芥,轉植株和野生型的葉型及花型並無顯著差異。而At243RNAi轉殖株及at243 T-DNA插入突變株(T-DNA insertion line)雖然在葉型及花型上也與野生型沒有差異,但卻發現有提早開花的現象,大約在8片簇生葉時就會開花,而野生型阿拉伯芥要在11~12片艚生葉時才會開花。以時間上來說,At243RNAi轉殖株及at243 T-DNA插入突變株比野生型提早6天開花。檢測FLC、FT、SOC1、GL1等和開花有關的基因,發現FLC在at243 T-DNA插入突變株中的表現量有減少,而FT及SOC1則增加,GL1的表現量和野生型沒有差異,表示提早開花的現象可能與FLC被抑制表現有關。FLC的表現會因為組蛋白H3-K4-trimethylation的程度變高而增加,且前人研究指出,At243可能是BRE1 orthologue,BRE1和組蛋白H2B ubiquitination有關,並進一步促使組蛋白H3 methylation。西方墨點分析的結果發現,At243RNAi轉殖株及at243突變株中組蛋白H3-K4-trimethylation的程度比野生型少,表示抑制At243表現會使得組蛋白H3-K4-trimethylation的程度變少,而這可能就造成At243RNAi轉殖株及at243突變株中FLC的表現被抑制,因而使植株提早開花。播種後40天的at243 T-DNA插入突變株葉綠素含量也比野生型少,表示at243 T-DNA插入突變株可能有提早老化的現象。 總的來說,大量表現OSAG243於阿拉伯芥並不會影響阿拉伯芥的生理功能,但抑制阿拉伯芥內生At243的表現可能會減少組蛋白H3-K4-trimethylation的程度並抑制FLC的表現,最終影響開花時間。 | zh_TW |
dc.description.abstract | The cutting-flower of Onicidium Gower Ramsey is the most important export flowers in Taiwan. However, the vase life of the onicidium cutting-flower is not long enough for better economical value. Therefore, how to delay the senescence of onicidium cutting-flower is the prior way to study. Our laboratory used several methods, including suppression subtractive hybridization, differential display, and protein 2D-PAGE, to isolate the senescence-associated genes (SAGs). OSAG243 is one of them, and results from real-time PCR revealed that OSAG243 is a senescence-associated gene. The full length cDNA of OSAG243 was isolated using Rapid Amplification of cDNA Ends (RACE). After OSAG243 was compared to database in NCBI, it showed that OSAG243 is a C3HC4-type RING finger protein. In order to understand the functions of OSAG243 in plant, Arabidopsis expressing OSAG243 driven by 35S promoter or AGL5 promoter was constructed. In terms of phenotype and dark-induced senescence, no significant difference between wild-type and transgenic Arabidopsis was observed. Surprisingly, the flowering time of At243RNAi and Arabidopsis with T-DNA insertion in At243 gene is seven days earlier than that of wild-type Arabidopsis. Semi-quantitative RT-PCR indicated that depression of FLC caused the early flowering phenotype. The chlorophyll content of 40-day-old At243 T-DNA insertion line was lower than that of wild-type Arabidopsis. It indicated that At243 T-DNA insertion line might be senescent earliler than wild-type Arabidopsis. Westren blot results indicated that knocking out At243 in Arabidopsis caused lower level of methylation in histone H3, and it would down-regulate the expression of FLC. Conclusively, overexpression of OSAG243, a RING finger protein, may not affect the physiological functions of Arabidopsis. However, knocking out the At243 in Arabidopsis may decrease methylation level of histone H3 and down-regulate the expression of FLC, and finally influence the timing of flowering. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:12:10Z (GMT). No. of bitstreams: 1 ntu-95-R93b42002-1.pdf: 6738663 bytes, checksum: 6a4c3bd115987bc5eb9984221ac66db9 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 中文摘要 i
英文摘要 ii 第一章 前言 一、文心蘭背景說明 1 二、植物之老化 2 三、Ubiquitin/26S proteasome pathway 5 四、阿拉伯芥開花調控路徑 6 五、實驗方向與目的 6 第二章 材料與方法 一、材料 8 二、文心蘭花瓣RNA的萃取 8 三、半定量RT-PCR 10 四、Rapid Amplification of cDNA Ends (RACE) 10 五、質粒構築與挑選 12 六、阿拉伯芥浸潤基因轉殖實驗 15 七、暗處理 17 八、阿拉伯芥histone的萃取 17 九、蛋白質SDS膠體電泳分析 18 十、西方墨點分析 20 十一、葉綠素含量測定 21 十二、Real-Time PCR 22 第三章 結果 一、文心蘭OSAG243基因的釣取及比對 23 二、大量表現OSAG243於阿拉伯芥 24 三、抑制At243在阿拉伯芥的表現 25 四、葉綠素含量測定 27 五、阿拉伯芥開花相關基因的檢測 28 六、At243可能是BRE1 orthologue 29 第四章 討論 一、OSAG243蛋白質序列分析 30 二、大量表現OSAG243於阿拉伯芥 31 三、抑制At243在阿拉伯芥的表現 32 四、at243 T-DNA插入突變株及At243RNAi轉殖株有提早開花的現象 33 五、at243 T-DNA插入突變株提早開花可能與FLC被抑制有關 34 六、at243 T-DNA插入突變株可能會提早老化 35 七、At243可能是BRE1 orthologue且可能與histone的修飾有關 36 八、FLC的表現與histone H3-K4 trimethylation的關係 38 九、結論 40 第五章 參考文獻 41 圖表 46 | |
dc.language.iso | zh-TW | |
dc.title | 文心蘭老化相關基因OSAG243的釣取與分析 | zh_TW |
dc.title | The isolation and analysis of OSAG243, an Oncidium senescence-associated gene | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張孟基,王淑美 | |
dc.subject.keyword | 文心蘭,老化, | zh_TW |
dc.subject.keyword | Oncidium,senescence, | en |
dc.relation.page | 73 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 6.58 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。