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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻 | |
dc.contributor.author | Hsiang-Chun Kuo | en |
dc.contributor.author | 郭香君 | zh_TW |
dc.date.accessioned | 2021-06-13T04:11:38Z | - |
dc.date.available | 2006-07-28 | |
dc.date.copyright | 2006-07-28 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2006-07-26 | |
dc.identifier.citation | 參考文獻
1. Haydon,D.A.,Seaman,G.V.“Electrokinetic studies on the ultrastructure of human erythrocyte Ι.Electrophoresis at high ionic strengh-the cell as a polyanion”, Arch Biochem Biophys.,122, 126-136,1967 2. Webb,K., Hlady,V.,Tresco,P.A.,“Relationships among cell attachment,spreading,cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces”.J Biomed Mater Res.,49, 362-368,2000 3. Krasteva,N.,Harms,N.,Albrecht,W.,Seifert,B.,Hopp,M.,Altankov,G., Groth,T.,“Membranes for biohybrid liver support system-investigations on hepatocyte attachment,morphology and growth”,Biomaterials,23,2467-2478,2002 4. Haydon,D.A.,Seaman,G.V.“Electrokinetic studies on the ultrastructure of human erythrocyte Ι.Electrophoresis at high ionic strengh-the cell as a polyanion”, Arch Biochem Biophys.,122, 126-136,1967 5. I.Sakurda,“Polyvinyl Acohol Fibers”,Marcel Dekker Inc.,New York,1985 6. 許元昱、李旺祚、郭文勵:組織學,合記圖書出版社,台北,1992 7. Dupin E,Le Douarin NM.“Development of melanocyte precursors from the vertebrate neural crest”.Oncogene ,22, 3016-3023,2003 8. Lecoin L.,Lahav R.,Dupin E.,Le Douarin N.,“Development of melanocytes from neural crest progenitors,in:Molecular Basis of Epithelial Appendage Morphogenesis”,Chuong CM,ED.,Austin,Texas,U.S.A.,R.G.Landes Company,pp.131-154,1998 9. Yaar M,Gilchrest BA.“Human melanocyte growth and differentiaion:a decade of new data”,J Invest Dermatol,97,611-617,1991 10. Yu ,H.S.,Wu, C.S.,Yu, C.L.,Kao, Y.H.,Chiou, M.H.,“Helium-neon laser irradiation stimulates migration and proliferation in melanocytes and induces repigmentation in segmental-type vitiligo”,J Invest Dermatol,120,56-64,2003 11. Grompe,M.,Finegold,M.J.,Liver stem cells.In Stem Cell Biology.,Cold Spring Harbor Laboratory Press,New York.pp,2001 12. Bucher,N.R.R.,Swaffield,M.N.,“The rate of incorporation of labeled thymidine into the deoxyribonucleic acid of regeneration rat liver in relation to the amount of liver excise”,Cancer Res.,24, 1611-1625, 1964 13. Fausto,N.,“Liver regeneration”,J Hepatol,32, 19-31,2000 14. Hunter,R.J.,“Foundations of Collid Science,vol.1”,Oxford Univ.Press,Oxford,1989 15. Shaw,D.J.,“Introduction to Collid and Surface Chemistry,4th ed”,Butterworth-Heinemann,Oxford,1993 16. Moyer,L.S.,“A suggested standard method for the investigation of electrophoresis”,J.Bacteriol.,31,531-546,1935 17. Seaman,G.V.,Heard,D.H.,“A microphoresis chamber of small volume for use with biological system”,Blood,18, 599-604,1961 18. Mironov,S.L.,Dolgaya,E.V.,“Surface charge of mammalian neurons as reveraled by micro-electrophoresis”,J.Membrane Biol.,86, 197-202,1985 19. Ruhenstroth-Bauer,G.,Sachetleben,P.,in:Bauer,K.F..(Ed.),Medizinische Grundlagenforschung Ⅱ,Georg Thieme Verlag,Stuttgart, 639-660,1959 20. Bangham,A.D.,Heard,D.H.,Flemans,R.,Seaman,G.V.“An apparatus for microelectrophoresis of small particles”,Nature, 182, 642-644 , 1958 21. Uzgiris,E.E.,Kaplan,J.H.,“Study of lymphocyte and erythrocyte electrophoretic mobility by laser Doppler spectroscopy”,Anal.Biochem.,60, 455-461,1974 22. Wilson,W.W.,Wade,M.M.,Holman,S.C.,Champlin,F.R.,“Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements”, J.Microbiological Methods,43, 153-164,2001 23. Hannig,K.,Krusmann,W.F.,“Application of carrier-free continuous electrophoresis to the separation of white blood cells from human blood”,Hoppe-Seyler’s Z Physiol Chem.,349,161-170,1968 24. Hannig,K.,“New aspects in preparative and analytical continuous free flow electrophoresis”,Electrophoresis,3, 235-243,1982 25. Hymer,W.C.,Barlow,G.H.,Blaisdell,S.J.,Ceveland,C.,Farrington,M., Feldmeier,M.,Grindeland,R.,Hatfield,J.M.,Lanham,J.W.,Lewis,M.L.,Morrison,D.R.,Olack,B.J.,Richman,D.W.,Rose,J.,Scharp,D.W., Snyder,R.S.,Swanson,C.A.,Todd,P.,Wilfinger,W.,“Contineous flow electrophoretic separation of proteins and cells from mammalian tissues”,Cell Biophys.,10,61-85,1987 26. Mehrishi,J.N.,Bauer,J.,“Electrophoresis of cells and the biological relevance of surface charge”,Electrophoresis,23,1984-1994,2002 27. Slivinsky,G.G.,Hymer,W.C.,Bauer,J.,Morrison,D.R.,“Celluar electrophetic mobility data:a first approach to a database”,Electrophoresis,18, 1109-1119,1997 28. Hymer,W.C.,Salada,T.,Cenci,R.,Krishnan,K.,Seaman,G.V.,Snyder,R.,Mastumiya,H.,Nagaoka,S.,“Bioprocessing in microgravity:applications of continuous flow electrophoresis to rat anterior pituitary particles”,J.Biotechnol.,47,353-365,1996 29. Li,Q.F.,Wang,D.Y.,“The differentiation of human gastric adenocarcinoma cell line MGc80-3 induced by dibutyryl camp in vitro”,Acta Biol.Exp.Sinica,23, 167-175,1990 30. Schaeffer,B.E.,Schaeffer,H.E.,Brick,I.,“Cell electrophores of amphibian blastula and gastrula cells;the relationship of surface charge and morphogenetic movement”,Dev.Biol.,34, 66-76,1973 31. Jucker,B.A.,Harms,H.,Zehnder,A.J.,“Adhesion of the positively charged bacterium Stenotrophomonas(Xanthomonas)maltophilia 70401 to glass and Teflon”,J. Bacteriol.,178, 5472-5479,1996 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32560 | - |
dc.description.abstract | 摘要
本實驗主要探討不同的細胞以體外培養的方式種植於不同的高分子薄膜上,利用細胞電泳實驗來觀察不同的細胞在親水性及疏水性高分子薄膜上貼附情形及高分子薄膜親水及疏水性質對細胞膜表面電位之影響。 從實驗的結果我們發現,在親水性聚乙烯醇(PVA)高分子薄膜上,細胞的貼附情形並不好。而在疏水性聚偏二氟乙烯(PVDF)或是組織培養聚苯乙烯(TCPS)上的貼附情形較好。而從細胞電泳的實驗中我們發現,培養的基材不同,其細胞的電泳運動性亦不同。亦即培養基材會造成細胞膜表面的電位不同,因而反應在其電泳運動性上。由實驗的結果顯示,當細胞種植於親水性基材PVA上一小時後,其運動性較高,亦即細胞種植於親水性基材時,其細胞表面帶電量較多。若將細胞種植於疏水性基材PVDF上,則其運動性相對的較低。即細胞培養於疏水性基材時,其表面帶電量較少。另外對同一種基材而言,貼附於基材上的細胞,其電泳運動性較未貼附於基材上者高。顯示細胞貼附於基材上時,其表面帶電量較多。另外藉由細胞電泳實驗亦可探討細胞與培養基材之間作用力的關係。 關鍵字:聚乙烯醇、聚偏二氟乙烯、細胞電泳 | zh_TW |
dc.description.abstract | ABSTRACT
In this study, various cells were cultured on different polymer membranes in vitro. We investigated the adhesion of different cells on hydrophilic and hydrophobic polymer membranes and the influence of that on the surface charges of the plasma membranes by microelectrophoresis technique. The results suggested that cell adhesion on the hydrophilic PVA membrane was not very well, but those on the hydrophobic PVDF or TCPS membrane were better. From the experiment of microelectrophoresis, cells cultured on different substrates have dissimilar electrophoretic mobility. This result indicates that the substrates will affect the surface charges of the plasma membranes and respondes to electrophoretic mobility. When cells were cultured on hydrophilic PVA substrate for one hour, the electrophoretic mobility was higher than that on the hydrophobic PVDF substrate. Moreover, the electrophoetic mobility of the cells adhered on substrate were higher than that of those cells suspended on substrate. Keyword:microelectrophoresis, PVA, PVDF, hydrophilic,hydrophobic | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T04:11:38Z (GMT). No. of bitstreams: 1 ntu-94-R93549033-1.pdf: 9129673 bytes, checksum: 04636d8dd51239d82a869cca96ba4c90 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄
摘要.................................................................................................Ι ABSTRACT.....................................................................................Π 目錄..................................................................................................Ⅲ 第一章 背景....................................................................................1 第二章 文獻回顧............................................................................6 2-1 細胞簡介......................................................................... ..6 2-1-1 黑色素細胞...............................................................6 2-1-2 肝臟細胞..................................................................10 2-1-3 神經幹細胞..............................................................14 2-1-4 角膜細胞和角膜上皮細胞......................................17 2-1-5 牙胚細胞..................................................................19 2-2 膠體溶液.............................................................................22 2-3 膠體粒子表面的電荷來源.................................................23 2-4 膠體電泳理論.....................................................................24 2-5 細胞電泳.............................................................................24 第三章 實驗器材與方法..............................................................28 3-1 實驗藥品...........................................................................28 3-2 實驗儀器...........................................................................29 3-3 試劑配製.........................................................................31 3-4 細胞電泳實驗.................................................................32 3-4-1 薄膜的前置處理....................................................32 3-4-2 電泳前細胞的前置處理........................................33 3-4-3 細胞電泳................................................................36 3-4-4 改變細胞種類並測量各種細胞的電泳運動性....39 3-4-5 改變培養基材並測量細胞的電泳運動性............40 3-4-6 胰蛋白酶和EDTA對細胞運動性的影響............40 第四章 實驗結果與討論...........................................................41 4-1胰蛋白酶和EDTA對細胞運動性的影響......................41 4-2 培養基材對細胞運動性的影響......................................42 第五章 結論.................................................................................48 參考文獻.......................................................................................50 附圖 圖2-1 皮膚結構圖..................................................................................7 圖2-2 表皮結構圖..................................................................................7 圖2-3 黑色素細胞的圖解......................................................................8 圖2-4 黑色素細胞中的黑色素形成過程..............................................9 圖2-5 肝臟結構圖................................................................................10 圖2-6 肝臟的示意圖............................................................................11 圖2-7 神經幹細胞的分化及生長圖....................................................17 圖2-8 角膜結構圖................................................................................18 圖2-9 牙齒分化過程圖........................................................................20 圖2-10 牙齒發育階段圖......................................................................22 圖3-1 細胞電泳實驗設備-電泳槽....................................................37 圖3-2 細胞電泳實驗裝置....................................................................38 圖4-1 不同細胞經胰蛋白酶處理後的電泳運動性............................54 圖4-2 人類肝臟腫瘤細胞種植在不同基材上一小時後的運動...….54 圖4-3 牙胚細胞種植在不同基材上一小時後的運動性…..…...…...55 圖4-4 角膜上皮細胞種植在不同基材上一小時後的運動性……....55 圖4-5 神經幹細胞種植在不同基材上一小時後的運動性………....56 圖4-6 黑色素細胞種植在不同基材上一小時後的運動性………....56 圖4-7 不同細胞種植在不同基材上一小時後的運動性…………...57 圖4-8 不同細胞種植在PVA上一小時後的運動性……………….57 圖4-9 不同細胞種植在PVDF上一小時後的運動性……………..58 圖4-10 不同細胞種植在TCPS上一小時後的運動性……..……...58 圖4-11 人類肝臟腫瘤細胞種植於PVA上一小時後的照片(a)100X (b)200X…………………………………………………..….59 圖4-12 人類肝臟腫瘤細胞種植於PVA上一小時後,將上層未貼附 於基材上的細胞懸浮液吸出後的照片(a)100X(b)200X .…59 圖4-13 人類肝臟腫瘤細胞種植於PVDF上一小時後的照片(a)100X (b)200X…………………………………………………..….60 圖4-14人類肝臟腫瘤細胞種植於PVDF上一小時後,將上層未貼 附於基材上的細胞懸浮液吸出後的照片(a)100X(b)200X.60 圖4-15人類肝臟腫瘤細胞種植於TCPS上一小時後的照片(a)100X (b)200X…………………………………………………..…..61 圖4-16 人類肝臟腫瘤細胞種植於TCPS上一小時後,將上層未貼附 於基材上的細胞懸浮液吸出後的照片(a)100X(b)200X.…..61 圖4-17 牙胚細胞種植於PVA上一小時後的照片(a)100X(b)200X..62 圖4-18 牙胚細胞種植於PVA上一小時後,將上層未貼附於基材上 的細胞懸浮液吸出後的照片(a)100X(b)200X……...….….....62 圖4-19 牙胚細胞種植於PVDF上一小時後的照片(a)100X (b)200X………………………………………………………..63 圖4-20 牙胚細胞種植於PVDF上一小時後,將上層未貼附於基材上 的細胞懸浮液吸出後的照片(a)100X(b)200X……………….63 圖4-21 牙胚細胞種植於TCPS上一小時後的照片(a)100X (b)200X………………………………………………………..64 圖4-22 牙胚細胞種植於TCPS上一小時後,將上層未貼附於基材上 的細胞懸浮液吸出後的照片(a)100X(b)200X……………….64 圖4-23 神經幹細胞種植於PVA上一小時後的照片(a)100X(b)200X (經胰蛋白酶處理,培養基含血清)………………….……65 圖4-24 神經幹細胞種植於PVA上一小時後,將上層未貼附於基材 上的細胞懸浮液吸出後的照片(a)100X(b)200X(經胰蛋白酶 處理,培養基含血清)…………………………………......65 圖4-25 神經幹細胞種植於PVDF上一小時後的照片(a)100X(b)200X (經胰蛋白酶處理,培養基含血清)……………………....66 圖4-26 神經幹細胞種植於PVDF上一小時後,將上層未貼附於基 材上的細胞懸浮液吸出後的照片(a)100X(b)200X(經胰蛋白 酶處理,培養基含血清)…………………………......……66 圖4-27 神經幹細胞種植於TCPS上一小時後的照片(a)100X(b)200X (經胰蛋白酶處理,培養基含血清)……………………….67 圖4-28 神經幹細胞種植於TCPS上一小時後,將上層未貼附於基材 上的細胞懸浮液吸出後的照片(a)100X(b)200X(經胰蛋白酶 處理,培養基含血清)……………………………………...67 圖4-29 黑色素細胞種植於PVA上一小時後的照片(a)100X (b)200X……………………………………………………….68 圖4-30 黑色素細胞種植於PVA上一小時後,將上層未貼附於基材 上的細胞懸浮液吸出後的照片(a)100X(b)200X…………...68 圖4-31 黑色素細胞種植於PVDF上一小時後的照片(a)100X (b)200X……………………………………………….………69 圖4-32 黑色素細胞種植於PVDF上一小時後,將上層未貼附於基 材上的細胞懸浮液吸出後的照片(a)100X(b)200X…….…...69 圖4-33 黑色素細胞種植於TCPS上一小時後的照片(a)100X (b)200X……………………………………………………….70 圖4-34 黑色素細胞種植於TCPS上一小時後,將上層未貼附於基 材上的細胞懸浮液吸出後的照片(a)100X(b)200X…….….70 圖4-35 角膜上皮細胞種植於PVA上一小時後的照片(a)100X (b)200X…………………………………………………...…..71 圖4-36 角膜上皮細胞種植於PVA上一小時後,將上層未貼附於 基材上的細胞懸浮液吸出後的照片(a)100X(b)200X….…...71 圖4-37 角膜上皮細胞種植於PVDF上一小時後的照片(a)100X (b)200X…………………………………………………....…..72 圖4-38角膜上皮細胞種植於PVDF上一小時後,將上層未貼附於 基材上的細胞懸浮液吸出後的照片(a)100X(b)200X…….....72 圖4-39 角膜上皮細胞種植於TCPS上一小時後的照片(a)100X (b)200X………………………………………………………..73 圖4-40 角膜上皮細胞種植於TCPS上一小時後,將上層未貼附於 基材上的細胞懸浮液吸出後的照片(a)100X(b)200X….…...73 圖5-1 細胞貼附於培養基材之示意圖…………………………….....74 圖5-2 培養基材與細胞膜對週邊蛋白吸引競爭力示意圖(基材對培 養基中蛋白質作用力大於對細胞膜蛋白質的作用力)….....75 圖5-3 培養基材與細胞膜對週邊蛋白吸引競爭力示意圖(基材對細 胞膜蛋白質作用力大於對培養基中蛋白質的作用力).........76 圖5-4 三個胚層的來源及其衍生..……………………………………77 圖5-5 中樞神經系統的分化過程……………..………………………78 圖5-6 神經脊的衍生物…………………………..……………………79 附表 表2-1 各種不同細胞的電泳運動性分布圖........................................80 表2-2 各胚層分化之眼睛構造………………………………………81 附錄 圖6-1 人類肝臟腫瘤細胞經胰蛋白酶處理後不同時間的電泳運動 性................................................................................................82 圖6-2 牙胚細胞經胰蛋白酶處理後不同時間的電泳運動性............82 圖6-3 角膜上皮細胞經胰蛋白酶處理後不同時間的電泳運動性....83 圖6-4 角膜細胞經胰蛋白酶處理後不同時間的電泳運動性............83 圖6-5 神經幹細胞經胰蛋白酶處理後不同時間的電泳運動性........84 圖6-6 黑色素細胞經胰蛋白酶處理後不同時間的電泳運動性........84 圖6-7 紅血球細胞經胰蛋白酶處理後不同時間的電泳運動性........85 圖6-8 神經幹細胞種植在不同基材上一小時後的運動性 (經trypsin 處理,無血清)…………………………………85 | |
dc.language.iso | zh-TW | |
dc.title | 不同細胞培養在親水性及疏水性高分子薄膜上電泳行為之研究 | zh_TW |
dc.title | The study of electrophoresis of various types of cells after cultured on PVA and PVDF membranes | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 尹相姝,徐治平,謝銘鈞 | |
dc.subject.keyword | 聚乙烯醇,聚偏二氟乙烯,細胞電泳, | zh_TW |
dc.subject.keyword | microelectrophoresis,PVA,PVDF,hydrophilic,hydrophobic, | en |
dc.relation.page | 85 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
顯示於系所單位: | 高分子科學與工程學研究所 |
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