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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蕭寧馨 | |
dc.contributor.author | Chia-Ling Hsieh | en |
dc.contributor.author | 謝佳玲 | zh_TW |
dc.date.accessioned | 2021-06-13T03:52:06Z | - |
dc.date.available | 2012-05-30 | |
dc.date.copyright | 2006-07-29 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-26 | |
dc.identifier.citation | 李慧玲 (2004) 以 Caco-2 細胞模式探討 Hepcidin 對鐵吸收相關分子表現之影響。國立台灣大學微生物與生化學研究所碩士論文。
陳翠英 (2005) 以 Caco-2 細胞株探討維生素 C 影響鐵吸收之新作用機制並評估某些市售食品之鐵生物利用率。 國立台灣大學微生物與生化學研究所碩士論文。 王麗琳 (2005) 以 Caco-2 細胞模式探討 ferric reductase 的電子來源與影響攝鐵能力之成分。 國立台灣大學微生物與生化學研究所碩士論文。 陳采雲 (2005) 利用FRAP方法分析台灣水果之抗氧化力。 國立台灣大學園藝研學研究所碩士論文。 Andrews, N. C. (2005). Understanding heme transport. N Engl J Med 353, 2508-2509. Atanasova, B., Mudway, I. S., Laftah, A. H., Latunde-Dada, G. O., McKie, A. T., Peters, T. J., Tzatchev, K. N., and Simpson, R. J. (2004). Duodenal ascorbate levels are changed in mice with altered iron metabolism. J Nutr 134, 501-505. Atanasova, B. D., Li, A. C., Bjarnason, I., Tzatchev, K. N., Simpson, R. J., Latunde-Dada, G. O., Van der Westhuizen, J., Vulpe, C. D., Anderson, G. J., Simpson, R. J., and McKie, A. T. (2005). Duodenal ascorbate and ferric reductase in human iron deficiency. Am J Clin Nutr. 2005 Jan;81(1):130-3 Bedrine-Ferran, H., Le Meur, N., Gicquel, I., Le Cunff, M., Soriano, N., Guisle, I., Mottier, S., Monnier, A., Teusan, R., Fergelot, P., et al. (2004). 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C., and Wood, R. J. (1999). Reciprocal regulation of HFE and NNamp2 gene expression by iron in human intestinal cells. J Nutr 129, 98-104. Latunde-Dada, G. O., Van der Westhuizen, J., Vulpe, C. D., Anderson, G. J., Simpson, R. J., and McKie, A. T. (2002). Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. Blood Cells Mol Dis 29, 356-360. Jorgensen, R. (1990). Altered gene expression in plants due to trans interactions between homologous genes. Trends Biotechnol 8, 340-344. Latunde-Dada, G. O., Van der Westhuizen, J., Vulpe, C. D., Anderson, G. J., Simpson, R. J., and McKie, A. T. (2002). Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. Blood Cells Mol Dis 29, 356-360. Kuo, S. M., MacLean, M. E., McCormick, K., and Wilson, J. X. (2004). Gender and sodium-ascorbate transporter isoforms determine ascorbate concentrations in mice. J Nutr 134, 2216-2221. Lee, R. C., Feinbaum, R. L., and Ambros, V. (1993). The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75, 843-854. Martini, L. A., Tchack, L., and Wood, R. J. (2002). Iron treatment downregulates DMT1 and IREG1 mRNA expression in Caco-2 cells. J Nutr 132, 693-696. Maulen, N. P., Henriquez, E. A., Kempe, S., Carcamo, J. G., Schmid-Kotsas, A., Bachem, M., Grunert, A., Bustamante, M. E., Nualart, F., and Vera, J. C. (2003). Up-regulation and polarized expression of the sodium-ascorbic acid transporter SVCT1 in post-confluent differentiated CaCo-2 cells. J Biol Chem 278, 9035-9041. McKie, A. T. (2005). A ferrireductase fills the gap in the transferrin cycle. Nat Genet 37, 1159-1160. McKie, A. T., Barrow, D., Latunde-Dada, G. O., Rolfs, A., Sager, G., Mudaly, E., Mudaly, M., Richardson, C., Barlow, D., Bomford, A., et al. (2001). An iron-regulated ferric reductase associated with the absorption of dietary iron. Science 291, 1755-1759. McKie, A. T., Latunde-Dada, G. O., Miret, S., McGregor, J. A., Anderson, G. J., Vulpe, C. D., Wrigglesworth, J. M., and Simpson, R. J. (2002). Molecular evidence for the role of a ferric reductase in iron transport. Biochem Soc Trans 30, 722-724. Millard, K. N., Frazer, D. M., Wilkins, S. J., and Anderson, G. J. (2004). Changes in the expression of intestinal iron transport and hepatic regulatory molecules explain the enhanced iron absorption associated with pregnancy in the rat. Gut 53, 655-660. Morris, K. V. (2005). siRNA-mediated transcriptional gene silencing: the potential mechanism and a possible role in the histone code. Cell Mol Life Sci 62, 3057-3066. Pinto, M., Robine-Leon, S., Appay, M. D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, J. & Zweibaum, A. (1983). Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47: 323-330 Pountney, D. J., Raja, K. B., Simpson, R. J., and Wrigglesworth, J. M. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32483 | - |
dc.description.abstract | Duodenal cytochrome b是小腸細胞膜上之三價鐵還原酵素(ferric reductase),其還原所需之電子可由維生素C提供。缺乏Dcytb的小鼠其體內鐵營養狀態與正常小鼠無顯著差別,反映Dcytb對於小鼠吸收三價鐵並無必需性。不同於小鼠,人類無法自行合成維生素C,因此Dcytb對於人類吸收三價鐵上的必需性需進一步探討。所以本研究利用人類大腸癌Caco-2細胞,探討Dcytb與鐵蛋白含量間之相關性,包括三個實驗:第一部份為比較2% FBS+DMEM及MEM中營養成分及攝鐵時間長短,兩項培養條件對ferric reductase活性及鐵蛋白量的影響;第二部分為比較還原力由高至低為桑椹、葡萄、奇異果、柳橙、蘋果之果汁對ferric reductase活性及鐵蛋白量的影響;第三部份為建立以siRNA抑制Caco-2細胞Dcytb基因表現之模式,及探討Dcytb 對Caco-2細胞於鐵吸收的機制上之必需性。此外,也探討還原力是否可作為評估鐵生物利用率之依據。第一部分結果顯示,以MEM為培養基進行攝鐵實驗,發現6及24小時後添加ascorbic acid (AA)組的酵素活性顯著增加,但6小時之鐵蛋白量與未添加組無差異,但24小時後其含量顯著上升。以2% FBS + DMEM為培養基,無論6及24小時,添加AA皆顯著增加酵素活性及鐵蛋白量;第二部分結果顯示,細胞ferric reductase活性由高至低為奇異果>桑椹>葡萄>柳橙、蘋果汁,鐵蛋白量由高至低為奇異果>桑椹>柳橙>葡萄>蘋果汁,且發現桑椹及柳橙汁並不能促進酵素活性,但鐵蛋白量皆顯著較只添加鐵源組高。第三部分結果顯示,保留30%之細胞Dcytb mRNA量,可能足以表現細胞70%之ferric reductase活性,而添加維生素C後,可能改善其ferric reductase活性降低之現象,使細胞可表現90%之ferric reductase活性。綜合上述,本研究發現duodenal cytochrome b可能對於人類小腸細胞攝鐵不具有必需性,但其缺乏表現時,仍可能影響鐵之生物利用率,不過可利用補充維生素C替代其降低之ferric reductase活性。此外,也發現並不能完全只用果汁的還原力作為評估鐵生物利用率之依據,可能還需依其組成主要成份來進行判斷。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:52:06Z (GMT). No. of bitstreams: 1 ntu-95-R93b47305-1.pdf: 1622012 bytes, checksum: 7b7552a8db975c19f8688e0c08c19ee9 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 第一章
文獻回顧 1 第一節 Duodenal cytochrome b 1 第二節 小腸細胞鐵吸收之分子機制 4 第三節 RNA interference 8 第四節 Caco-2 細胞模式 11 第五節 研究目的與假說 15 第二章 Caco-2細胞培養條件對ferric reductase 活性及鐵蛋白合成量之影響 第一節 材料與方法 17 第二節 結果 28 一、 培養液對鐵分佈型式之影響 二、 培養液對細胞存活率之影響 三、 培養液對細胞攝鐵實驗時間之影響 四、 血清及營養素含量對細胞存活率之影響 五、 血清及營養素含量對細胞ferric reductase 活性及鐵蛋白合成量之影響 第三節 討論 31 第四節 結論 33 第三章 果汁抗氧化力對ferric reductase 活性及鐵蛋白合成量之影響 第一節 材料與方法 41 第二節 結果 45 一、 柳橙汁中維生素C對細胞 ferric reductase 活性及鐵蛋白合成量之影響 二、 果汁之抗氧化力對細胞 ferric reductase 活性及鐵蛋白合成量之影響 第三節 討論 46 第四節 結論 48 第四章 利用siRNA抑制細胞Dcytb之基因表現對鐵吸收之影響 第一節 材料與方法 53 第二節 結果 64 一、 Dcytb 及T7-Dcytb cDNA選殖 二、 Dcytb-siRNA Library之構築 三、 轉染Dcytb-siRNA對細胞Dcytb mRNA表現之影響 四、 抑制細胞Dcytb mRNA表現對ferric reductase 活性及鐵蛋白合成量之影響 第三節 討論 67 第四節 結論 69 第五章 討論與總結 79 第六章 參考文獻 84 | |
dc.language.iso | zh-TW | |
dc.title | 以 Caco-2 細胞模式探討
ferric reductase 的活性與小腸攝鐵之必要性 | zh_TW |
dc.title | The essential role of ferric reductase on intestinal iron absorption by a Caco-2 cell model | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王果行,何素珍,楊雯如,沈立言 | |
dc.subject.keyword | Caco-2 細胞,ferric reductase,鐵蛋白, | zh_TW |
dc.subject.keyword | Caco-2 cell,ferric reductase,ferritin, | en |
dc.relation.page | 90 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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