請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32431
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張鑫 | |
dc.contributor.author | Chiao-Ling Chang | en |
dc.contributor.author | 張巧伶 | zh_TW |
dc.date.accessioned | 2021-06-13T03:48:57Z | - |
dc.date.available | 2006-08-03 | |
dc.date.copyright | 2006-08-03 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-25 | |
dc.identifier.citation | Asabe, S.I., Tanji, Y., Satoh, S., Kaneko, T., Kimura, K. and Shimotohno, K. (1997) The N-terminal region of hepatitis C virus-encoded NS5A is important for NS4A-dependent phosphorylation. J Virol, 71, 790-796.
Bartenschlager, R., Ahlborn-Laake, L., Mous, J. and Jacobsen, H. (1993) Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions. J Virol, 67, 3835-3844. Bartenschlager, R., Ahlborn-Laake, L., Mous, J. and Jacobsen, H. (1994) Kinetic and structural analyses of hepatitis C virus polyprotein processing. J Virol, 68, 5045-5055. Bradley, D.W., McCaustland, K.A., Cook, E.H., Schable, C.A., Ebert, J.W. and Maynard, J.E. (1985) Posttransfusion non-A, non-B hepatitis in chimpanzees. Physicochemical evidence that the tubule-forming agent is a small, enveloped virus. Gastroenterology, 88, 773-779. Bretner, M. (2005) Existing and future therapeutic options for hepatitis C virus infection. Acta Biochim Pol, 52, 57-70. Brown, E.A., Zhang, H., Ping, L.H. and Lemon, S.M. (1992) Secondary structure of the 5' nontranslated regions of hepatitis C virus and pestivirus genomic RNAs. Nucleic Acids Res, 20, 5041-5045. Brown, K.J. and Parish, C.R. (1994) Histidine-rich glycoprotein and platelet factor 4 mask heparan sulfate proteoglycans recognized by acidic and basic fibroblast growth factor. Biochemistry, 33, 13918-13927. Bukh, J., Purcell, R.H. and Miller, R.H. (1994) Sequence analysis of the core gene of 14 hepatitis C virus genotypes. Proc Natl Acad Sci U S A, 91, 8239-8243. Carrere-Kremer, S., Montpellier-Pala, C., Cocquerel, L., Wychowski, C., Penin, F. and Dubuisson, J. (2002) Subcellular localization and topology of the p7 polypeptide of hepatitis C virus. J Virol, 76, 3720-3730. Chang, J., Yang, S.H., Cho, Y.G., Hwang, S.B., Hahn, Y.S. and Sung, Y.C. (1998) Hepatitis C virus core from two different genotypes has an oncogenic potential but is not sufficient for transforming primary rat embryo fibroblasts in cooperation with the H-ras oncogene. J Virol, 72, 3060-3065. Chang, K.S. and Luo, G. (2006) The polypyrimidine tract-binding protein (PTB) is required for efficient replication of hepatitis C virus (HCV) RNA. Virus Res, 115, 1-8. Chang, S.C., Cheng, J.C., Kou, Y.H., Kao, C.H., Chiu, C.H., Wu, H.Y. and Chang, M.F. (2000) Roles of the AX(4)GKS and arginine-rich motifs of hepatitis C virus RNA helicase in ATP- and viral RNA-binding activity. J Virol, 74, 9732-9737. Chang, S.C., Yen, J.H., Kang, H.Y., Jang, M.H. and Chang, M.F. (1994) Nuclear localization signals in the core protein of hepatitis C virus. Biochem Biophys Res Commun, 205, 1284-1290. Chen, C.M., You, L.R., Hwang, L.H. and Lee, Y.H. (1997) Direct interaction of hepatitis C virus core protein with the cellular lymphotoxin-beta receptor modulates the signal pathway of the lymphotoxin-beta receptor. J Virol, 71, 9417-9426. Cheng, J.C., Chang, M.F. and Chang, S.C. (1999) Specific interaction between the hepatitis C virus NS5B RNA polymerase and the 3' end of the viral RNA. J Virol, 73, 7044-7049. Choo, Q.L., Kuo, G., Weiner, A.J., Overby, L.R., Bradley, D.W. and Houghton, M. (1989) Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science, 244, 359-362. Choo, Q.L., Richman, K.H., Han, J.H., Berger, K., Lee, C., Dong, C., Gallegos, C., Coit, D., Medina-Selby, R., Barr, P.J. and et al. (1991) Genetic organization and diversity of the hepatitis C virus. Proc Natl Acad Sci U S A, 88, 2451-2455. Chou, A.H., Tsai, H.F., Wu, Y.Y., Hu, C.Y., Hwang, L.H., Hsu, P.I. and Hsu, P.N. (2005) Hepatitis C virus core protein modulates TRAIL-mediated apoptosis by enhancing Bid cleavage and activation of mitochondria apoptosis signaling pathway. J Immunol, 174, 2160-2166. Chung, R.T. and Kaplan, L.M. (1999) Heterogeneous nuclear ribonucleoprotein I (hnRNP-I/PTB) selectively binds the conserved 3' terminus of hepatitis C viral RNA. Biochem Biophys Res Commun, 254, 351-362. Corrigan, J.J., Jr., Jeter, M.A., Bruck, D. and Feinberg, W.M. (1990) Histidine-rich glycoprotein levels in children: the effect of age. Thromb Res, 59, 681-686. Deleersnyder, V., Pillez, A., Wychowski, C., Blight, K., Xu, J., Hahn, Y.S., Rice, C.M. and Dubuisson, J. (1997) Formation of native hepatitis C virus glycoprotein complexes. J Virol, 71, 697-704. Donate, F., Juarez, J.C., Guan, X., Shipulina, N.V., Plunkett, M.L., Tel-Tsur, Z., Shaw, D.E., Morgan, W.T. and Mazar, A.P. (2004) Peptides derived from the histidine-proline domain of the histidine-proline-rich glycoprotein bind to tropomyosin and have antiangiogenic and antitumor activities. Cancer Res, 64, 5812-5817. Drasin, T. and Sahud, M. (1996) Blood-type and age affect human plasma levels of histidine-rich glycoprotein in a large population. Thromb Res, 84, 179-188. Dubuisson, J., Penin, F. and Moradpour, D. (2002) Interaction of hepatitis C virus proteins with host cell membranes and lipids. Trends Cell Biol, 12, 517-523. Egger, D., Wolk, B., Gosert, R., Bianchi, L., Blum, H.E., Moradpour, D. and Bienz, K. (2002) Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex. J Virol, 76, 5974-5984. Einav, S., Elazar, M., Danieli, T. and Glenn, J.S. (2004) A nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication. J Virol, 78, 11288-11295. El-Hage, N. and Luo, G. (2003) Replication of hepatitis C virus RNA occurs in a membrane-bound replication complex containing nonstructural viral proteins and RNA. J Gen Virol, 84, 2761-2769. Elazar, M., Liu, P., Rice, C.M. and Glenn, J.S. (2004) An N-terminal amphipathic helix in hepatitis C virus (HCV) NS4B mediates membrane association, correct localization of replication complex proteins, and HCV RNA replication. J Virol, 78, 11393-11400. Failla, C., Tomei, L. and De Francesco, R. (1994) Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. J Virol, 68, 3753-3760. Failla, C., Tomei, L. and De Francesco, R. (1995) An amino-terminal domain of the hepatitis C virus NS3 protease is essential for interaction with NS4A. J Virol, 69, 1769-1777. Farci, P., Alter, H.J., Govindarajan, S., Wong, D.C., Engle, R., Lesniewski, R.R., Mushahwar, I.K., Desai, S.M., Miller, R.H., Ogata, N. and et al. (1992) Lack of protective immunity against reinfection with hepatitis C virus. Science, 258, 135-140. Florese, R.H., Nagano-Fujii, M., Iwanaga, Y., Hidajat, R. and Hotta, H. (2002) Inhibition of protein synthesis by the nonstructural proteins NS4A and NS4B of hepatitis C virus. Virus Res, 90, 119-131. Friebe, P. and Bartenschlager, R. (2002) Genetic analysis of sequences in the 3' nontranslated region of hepatitis C virus that are important for RNA replication. J Virol, 76, 5326-5338. Gale, M., Jr., Blakely, C.M., Kwieciszewski, B., Tan, S.L., Dossett, M., Tang, N.M., Korth, M.J., Polyak, S.J., Gretch, D.R. and Katze, M.G. (1998) Control of PKR protein kinase by hepatitis C virus nonstructural 5A protein: molecular mechanisms of kinase regulation. Mol Cell Biol, 18, 5208-5218. Gale, M.J., Jr., Korth, M.J., Tang, N.M., Tan, S.L., Hopkins, D.A., Dever, T.E., Polyak, S.J., Gretch, D.R. and Katze, M.G. (1997) Evidence that hepatitis C virus resistance to interferon is mediated through repression of the PKR protein kinase by the nonstructural 5A protein. Virology, 230, 217-227. Gao, L., Aizaki, H., He, J.W. and Lai, M.M. (2004) Interactions between viral nonstructural proteins and host protein hVAP-33 mediate the formation of hepatitis C virus RNA replication complex on lipid raft. J Virol, 78, 3480-3488. Glue, P., Rouzier-Panis, R., Raffanel, C., Sabo, R., Gupta, S.K., Salfi, M., Jacobs, S. and Clement, R.P. (2000) A dose-ranging study of pegylated interferon alfa-2b and ribavirin in chronic hepatitis C. The Hepatitis C Intervention Therapy Group. Hepatology, 32, 647-653. Gontarek, R.R., Gutshall, L.L., Herold, K.M., Tsai, J., Sathe, G.M., Mao, J., Prescott, C. and Del Vecchio, A.M. (1999) hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3'NTR of the HCV RNA genome. Nucleic Acids Res, 27, 1457-1463. Grakoui, A., McCourt, D.W., Wychowski, C., Feinstone, S.M. and Rice, C.M. (1993a) Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. J Virol, 67, 2832-2843. Grakoui, A., McCourt, D.W., Wychowski, C., Feinstone, S.M. and Rice, C.M. (1993b) A second hepatitis C virus-encoded proteinase. Proc Natl Acad Sci U S A, 90, 10583-10587. Griffin, S.D., Beales, L.P., Clarke, D.S., Worsfold, O., Evans, S.D., Jaeger, J., Harris, M.P. and Rowlands, D.J. (2003) The p7 protein of hepatitis C virus forms an ion channel that is blocked by the antiviral drug, Amantadine. FEBS Lett, 535, 34-38. Han, J.H., Shyamala, V., Richman, K.H., Brauer, M.J., Irvine, B., Urdea, M.S., Tekamp-Olson, P., Kuo, G., Choo, Q.L. and Houghton, M. (1991) Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5' untranslated region and poly(A) tails at the 3' end. Proc Natl Acad Sci U S A, 88, 1711-1715. Hayashi, J., Aoki, H., Arakawa, Y. and Hino, O. (1999) Hepatitis C virus and hepatocarcinogenesis. Intervirology, 42, 205-210. Hijikata, M., Mizushima, H., Akagi, T., Mori, S., Kakiuchi, N., Kato, N., Tanaka, T., Kimura, K. and Shimotohno, K. (1993) Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. J Virol, 67, 4665-4675. Hu, K.Q., Vierling, J.M. and Redeker, A.G. (2001) Viral, host and interferon-related factors modulating the effect of interferon therapy for hepatitis C virus infection. J Viral Hepat, 8, 1-18. Hugle, T., Fehrmann, F., Bieck, E., Kohara, M., Krausslich, H.G., Rice, C.M., Blum, H.E. and Moradpour, D. (2001) The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein. Virology, 284, 70-81. Ishido, S., Fujita, T. and Hotta, H. (1998) Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus. Biochem Biophys Res Commun, 244, 35-40. Jones, A.L., Hulett, M.D. and Parish, C.R. (2004) Histidine-rich glycoprotein binds to cell-surface heparan sulfate via its N-terminal domain following Zn2+ chelation. J Biol Chem, 279, 30114-30122. Jones, A.L., Hulett, M.D. and Parish, C.R. (2005) Histidine-rich glycoprotein: A novel adaptor protein in plasma that modulates the immune, vascular and coagulation systems. Immunol Cell Biol, 83, 106-118. Kaito, M., Watanabe, S., Tsukiyama-Kohara, K., Yamaguchi, K., Kobayashi, Y., Konishi, M., Yokoi, M., Ishida, S., Suzuki, S. and Kohara, M. (1994) Hepatitis C virus particle detected by immunoelectron microscopic study. J Gen Virol, 75 ( Pt 7), 1755-1760. Kaneko, T., Tanji, Y., Satoh, S., Hijikata, M., Asabe, S., Kimura, K. and Shimotohno, K. (1994) Production of two phosphoproteins from the NS5A region of the hepatitis C viral genome. Biochem Biophys Res Commun, 205, 320-326. Kato, J., Kato, N., Yoshida, H., Ono-Nita, S.K., Shiratori, Y. and Omata, M. (2002) Hepatitis C virus NS4A and NS4B proteins suppress translation in vivo. J Med Virol, 66, 187-199. Kato, N., Yoshida, H., Kioko Ono-Nita, S., Kato, J., Goto, T., Otsuka, M., Lan, K., Matsushima, K., Shiratori, Y. and Omata, M. (2000) Activation of intracellular signaling by hepatitis B and C viruses: C-viral core is the most potent signal inducer. Hepatology, 32, 405-412. Koide, T., Foster, D., Yoshitake, S. and Davie, E.W. (1986) Amino acid sequence of human histidine-rich glycoprotein derived from the nucleotide sequence of its cDNA. Biochemistry, 25, 2220-2225. Kolykhalov, A.A., Feinstone, S.M. and Rice, C.M. (1996) Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA. J Virol, 70, 3363-3371. Kolykhalov, A.A., Mihalik, K., Feinstone, S.M. and Rice, C.M. (2000) Hepatitis C virus-encoded enzymatic activities and conserved RNA elements in the 3' nontranslated region are essential for virus replication in vivo. J Virol, 74, 2046-2051. Kuo, G., Choo, Q.L., Alter, H.J., Gitnick, G.L., Redeker, A.G., Purcell, R.H., Miyamura, T., Dienstag, J.L., Alter, M.J., Stevens, C.E. and et al. (1989) An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science, 244, 362-364. Lanford, R.E. and Bigger, C. (2002) Advances in model systems for hepatitis C virus research. Virology, 293, 1-9. Le, S.Y., Liu, W.M. and Maizel, J.V., Jr. (1998) Phylogenetic evidence for the improved RNA higher-order structure in internal ribosome entry sequences of HCV and pestiviruses. Virus Genes, 17, 279-295. Leebeek, F.W., Kluft, C., Knot, E.A. and De Maat, M.P. (1989) Histidine-rich glycoprotein is elevated in mild liver cirrhosis and decreased in moderate and severe liver cirrhosis. J Lab Clin Med, 113, 493-497. Leung, L.L., Harpel, P.C., Nachman, R.L. and Rabellino, E.M. (1983) Histidine-rich glycoprotein is present in human platelets and is released following thrombin stimulation. Blood, 62, 1016-1021. Li, W., Kedersha, N., Chen, S., Gilks, N., Lee, G. and Anderson, P. (2004a) FAST is a BCL-X(L)-associated mitochondrial protein. Biochem Biophys Res Commun, 318, 95-102. Li, W., Simarro, M., Kedersha, N. and Anderson, P. (2004b) FAST is a survival protein that senses mitochondrial stress and modulates TIA-1-regulated changes in protein expression. Mol Cell Biol, 24, 10718-10732. Lin, C., Wu, J.W., Hsiao, K. and Su, M.S. (1997) The hepatitis C virus NS4A protein: interactions with the NS4B and NS5A proteins. J Virol, 71, 6465-6471. Lindenbach, B.D., Evans, M.J., Syder, A.J., Wolk, B., Tellinghuisen, T.L., Liu, C.C., Maruyama, T., Hynes, R.O., Burton, D.R., McKeating, J.A. and Rice, C.M. (2005) Complete replication of hepatitis C virus in cell culture. Science, 309, 623-626. Lohmann, V., Korner, F., Koch, J., Herian, U., Theilmann, L. and Bartenschlager, R. (1999) Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science, 285, 110-113. Lukavsky, P.J., Otto, G.A., Lancaster, A.M., Sarnow, P. and Puglisi, J.D. (2000) Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function. Nat Struct Biol, 7, 1105-1110. Lundin, M., Monne, M., Widell, A., Von Heijne, G. and Persson, M.A. (2003) Topology of the membrane-associated hepatitis C virus protein NS4B. J Virol, 77, 5428-5438. Luo, G. (1999) Cellular proteins bind to the poly(U) tract of the 3' untranslated region of hepatitis C virus RNA genome. Virology, 256, 105-118. Luo, S., Hall, A.E., Hall, S.E. and Preuss, D. (2004) Whole-genome fractionation rapidly purifies DNA from centromeric regions. Nat Methods, 1, 67-71. Macdonald, A., Crowder, K., Street, A., McCormick, C., Saksela, K. and Harris, M. (2003) The hepatitis C virus non-structural NS5A protein inhibits activating protein-1 function by perturbing ras-ERK pathway signaling. J Biol Chem, 278, 17775-17784. Macdonald, A. and Harris, M. (2004) Hepatitis C virus NS5A: tales of a promiscuous protein. J Gen Virol, 85, 2485-2502. MacFarlane, M. and Williams, A.C. (2004) Apoptosis and disease: a life or death decision. EMBO Rep, 5, 674-678. Matsumoto, M., Hsieh, T.Y., Zhu, N., VanArsdale, T., Hwang, S.B., Jeng, K.S., Gorbalenya, A.E., Lo, S.Y., Ou, J.H., Ware, C.F. and Lai, M.M. (1997) Hepatitis C virus core protein interacts with the cytoplasmic tail of lymphotoxin-beta receptor. J Virol, 71, 1301-1309. McLauchlan, J. (2000) Properties of the hepatitis C virus core protein: a structural protein that modulates cellular processes. J Viral Hepat, 7, 2-14. Mercer, D.F., Schiller, D.E., Elliott, J.F., Douglas, D.N., Hao, C., Rinfret, A., Addison, W.R., Fischer, K.P., Churchill, T.A., Lakey, J.R., Tyrrell, D.L. and Kneteman, N.M. (2001) Hepatitis C virus replication in mice with chimeric human livers. Nat Med, 7, 927-933. Meunier, J.C., Fournillier, A., Choukhi, A., Cahour, A., Cocquerel, L., Dubuisson, J. and Wychowski, C. (1999) Analysis of the glycosylation sites of hepatitis C virus (HCV) glycoprotein E1 and the influence of E1 glycans on the formation of the HCV glycoprotein complex. J Gen Virol, 80 ( Pt 4), 887-896. Moradpour, D. and Blum, H.E. (2004) A primer on the molecular virology of hepatitis C. Liver Int, 24, 519-525. Moradpour, D., Gosert, R., Egger, D., Penin, F., Blum, H.E. and Bienz, K. (2003) Membrane association of hepatitis C virus nonstructural proteins and identification of the membrane alteration that harbors the viral replication complex. Antiviral Res, 60, 103-109. Morgan, W.T. (1986) Serum histidine-rich glycoprotein levels are decreased in acquired immune deficiency syndrome and by steroid therapy. Biochem Med Metab Biol, 36, 210-213. Mori, S., Shinohata, R., Renbutsu, M., Takahashi, H.K., Fang, Y.I., Yamaoka, K., Okamoto, M., Yamamoto, I. and Nishibori, M. (2003) Histidine-rich glycoprotein plus zinc reverses growth inhibition of vascular smooth muscle cells by heparin. Cell Tissue Res, 312, 353-359. Nasoff, M.S., Zebedee, S.L., Inchauspe, G. and Prince, A.M. (1991) Identification of an immunodominant epitope within the capsid protein of hepatitis C virus. Proc Natl Acad Sci U S A, 88, 5462-5466. Neddermann, P., Tomei, L., Steinkuhler, C., Gallinari, P., Tramontano, A. and De Francesco, R. (1997) The nonstructural proteins of the hepatitis C virus: structure and functions. Biol Chem, 378, 469-476. Neumann, A.U., Lam, N.P., Dahari, H., Gretch, D.R., Wiley, T.E., Layden, T.J. and Perelson, A.S. (1998) Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-alpha therapy. Science, 282, 103-107. Nomura-Takigawa, Y., Nagano-Fujii, M., Deng, L., Kitazawa, S., Ishido, S., Sada, K. and Hotta, H. (2006) Non-structural protein 4A of Hepatitis C virus accumulates on mitochondria and renders the cells prone to undergoing mitochondria-mediated apoptosis. J Gen Virol, 87, 1935-1945. Park, J.S., Yang, J.M. and Min, M.K. (2000) Hepatitis C virus nonstructural protein NS4B transforms NIH3T3 cells in cooperation with the Ha-ras oncogene. Biochem Biophys Res Commun, 267, 581-587. Pavlovic, D., Neville, D.C., Argaud, O., Blumberg, B., Dwek, R.A., Fischer, W.B. and Zitzmann, N. (2003) The hepatitis C virus p7 protein forms an ion channel that is inhibited by long-alkyl-chain iminosugar derivatives. Proc Natl Acad Sci U S A, 100, 6104-6108. Pawlotsky, J.M. (1998) Hepatitis C virus infection: virus/host interactions. J Viral Hepat, 5 Suppl 1, 3-8. Pawlotsky, J.M. (2004) Pathophysiology of hepatitis C virus infection and related liver disease. Trends Microbiol, 12, 96-102. Penin, F., Dubuisson, J., Rey, F.A., Moradpour, D. and Pawlotsky, J.M. (2004) Structural biology of hepatitis C virus. Hepatology, 39, 5-19. Pflugheber, J., Fredericksen, B., Sumpter, R., Jr., Wang, C., Ware, F., Sodora, D.L. and Gale, M., Jr. (2002) Regulation of PKR and IRF-1 during hepatitis C virus RNA replication. Proc Natl Acad Sci U S A, 99, 4650-4655. Piccininni, S., Varaklioti, A., Nardelli, M., Dave, B., Raney, K.D. and McCarthy, J.E. (2002) Modulation of the hepatitis C virus RNA-dependent RNA polymerase activity by the non-structural (NS) 3 helicase and the NS4B membrane protein. J Biol Chem, 277, 45670-45679. Ray, R.B., Ghosh, A.K., Meyer, K. and Ray, R. (1999) Functional analysis of a transrepressor domain in the hepatitis C virus core protein. Virus Res, 59, 211-217. Ray, R.B., Lagging, L.M., Meyer, K., Steele, R. and Ray, R. (1995) Transcriptional regulation of cellular and viral promoters by the hepatitis C virus core protein. Virus Res, 37, 209-220. Reddy, K.R., Wright, T.L., Pockros, P.J., Shiffman, M., Everson, G., Reindollar, R., Fried, M.W., Purdum, P.P., 3rd, Jensen, D., Smith, C., Lee, W.M., Boyer, T.D., Lin, A., Pedder, S. and DePamphilis, J. (2001) Efficacy and safety of pegylated (40-kd) interferon alpha-2a compared with interferon alpha-2a in noncirrhotic patients with chronic hepatitis C. Hepatology, 33, 433-438. Saito, H., Goodnough, L.T., Boyle, J.M. and Heimburger, N. (1982) Reduced histidine-rich glycoprotein levels in plasma of patients with advanced liver cirrhosis. Possible implications for enhanced fibrinolysis. Am J Med, 73, 179-182. Saito, I., Miyamura, T., Ohbayashi, A., Harada, H., Katayama, T., Kikuchi, S., Watanabe, Y., Koi, S., Onji, M., Ohta, Y. and et al. (1990) Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci U S A, 87, 6547-6549. Santolini, E., Migliaccio, G. and La Monica, N. (1994) Biosynthesis and biochemical properties of the hepatitis C virus core protein. J Virol, 68, 3631-3641. Santolini, E., Pacini, L., Fipaldini, C., Migliaccio, G. and Monica, N. (1995) The NS2 protein of hepatitis C virus is a transmembrane polypeptide. J Virol, 69, 7461-7471. Shatsky, M., Saigo, K., Burdach, S., Leung, L.L. and Levitt, L.J. (1989) Histidine-rich glycoprotein blocks T cell rosette formation and modulates both T cell activation and immunoregulation. J Biol Chem, 264, 8254-8259. Shi, S.T., Lee, K.J., Aizaki, H., Hwang, S.B. and Lai, M.M. (2003) Hepatitis C virus RNA replication occurs on a detergent-resistant membrane that cofractionates with caveolin-2. J Virol, 77, 4160-4168. Siavoshian, S., Abraham, J.D., Thumann, C., Kieny, M.P. and Schuster, C. (2005) Hepatitis C virus core, NS3, NS5A, NS5B proteins induce apoptosis in mature dendritic cells. J Med Virol, 75, 402-411. Simantov, R., Febbraio, M., Crombie, R., Asch, A.S., Nachman, R.L. and Silverstein, R.L. (2001) Histidine-rich glycoprotein inhibits the antiangiogenic effect of thrombospondin-1. J Clin Invest, 107, 45-52. Simmonds, P. (2001) Reconstructing the origins of human hepatitis viruses. Philos Trans R Soc Lond B Biol Sci, 356, 1013-1026. Sizova, D.V., Kolupaeva, V.G., Pestova, T.V., Shatsky, I.N. and Hellen, C.U. (1998) Specific interaction of eukaryotic translation initiation factor 3 with the 5' nontranslated regions of hepatitis C virus and classical swine fever virus RNAs. J Virol, 72, 4775-4782. Smith, D.B., Mellor, J., Jarvis, L.M., Davidson, F., Kolberg, J., Urdea, M., Yap, P.L. and Simmonds, P. (1995) Variation of the hepatitis C virus 5' non-coding region: implications for secondary structure, virus detection and typing. The International HCV Collaborative Study Group. J Gen Virol, 76 ( Pt 7), 1749-1761. Spahn, C.M., Kieft, J.S., Grassucci, R.A., Penczek, P.A., Zhou, K., Doudna, J.A. and Frank, J. (2001) Hepatitis C virus IRES RNA-induced changes in the conformation of the 40s ribosomal subunit. Science, 291, 1959-1962. Street, A., Macdonald, A., Crowder, K. and Harris, M. (2004) The Hepatitis C virus NS5A protein activates a phosphoinositide 3-kinase-dependent survival signaling cascade. J Biol Chem, 279, 12232-12241. Tai, C.L., Chi, W.K., Chen, D.S. and Hwang, L.H. (1996) The helicase activity associated with hepatitis C virus nonstructural protein 3 (NS3). J Virol, 70, 8477-8484. Takahashi, K., Kishimoto, S., Yoshizawa, H., Okamoto, H., Yoshikawa, A. and Mishiro, S. (1992) p26 protein and 33-nm particle associated with nucleocapsid of hepatitis C virus recovered from the circulation of infected hosts. Virology, 191, 431-434. Tan, S.L. and Katze, M.G. (2001) How hepatitis C virus counteracts the interferon response: the jury is still out on NS5A. Virology, 284, 1-12. Tan, S.L., Pause, A., Shi, Y. and Sonenberg, N. (2002) Hepatitis C therapeutics: current status and emerging strategies. Nat Rev Drug Discov, 1, 867-881. Tanaka, T., Kato, N., Cho, M.J., Sugiyama, K. and Shimotohno, K. (1996) Structure of the 3' terminus of the hepatitis C virus genome. J Virol, 70, 3307-3312. Tanji, Y., Kaneko, T., Satoh, S. and Shimotohno, K. (1995) Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A. J Virol, 69, 3980-3986. Tardif, K.D., Mori, K. and Siddiqui, A. (2002) Hepatitis C virus subgenomic replicons induce endoplasmic reticulum stress activating an intracellular signaling pathway. J Virol, 76, 7453-7459. Taylor, D.R., Shi, S.T., Romano, P.R., Barber, G.N. and Lai, M.M. (1999) Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Science, 285, 107-110. Thomson, B.J. and Finch, R.G. (2005) Hepatitis C virus infection. Clin Microbiol Infect, 11, 86-94. Tian, Q., Taupin, J., Elledge, S., Robertson, M. and Anderson, P. (1995) Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis. J Exp Med, 182, 865-874. Togawa, A., Inoue, N., Shimoda, M., Mannoji, M., Yawata, Y., Miyamoto, K. and Namba, M. (1982) [Establishment and characterization of a human myeloma cell line (KMM-1)]. Nippon Ketsueki Gakkai Zasshi, 45, 607-614. Tong, M.J., Hwang, S.J., Lefkowitz, M., Lee, S.D., Co, R.L., Conrad, A., Schmid, P. and Lo, K.J. (1994) Correlation of serum HCV RNA and alanine aminotransferase levels in chronic hepatitis C patients during treatment with ribavirin. J Gastroenterol Hepatol, 9, 587-591. Tong, W.Y., Nagano-Fujii, M., Hidajat, R., Deng, L., Takigawa, Y. and Hotta, H. (2002) Physical interaction between hepatitis C virus NS4B protein and CREB-RP/ATF6beta. Biochem Biophys Res Commun, 299, 366-372. Tsukiyama-Kohara, K., Iizuka, N., Kohara, M. and Nomoto, A. (1992) Internal ribosome entry site within hepatitis C virus RNA. J Virol, 66, 1476-1483. Varaklioti, A., Vassilaki, N., Georgopoulou, U. and Mavromara, P. (2002) Alternate translation occurs within the core coding region of the hepatitis C viral genome. J Biol Chem, 277, 17713-17721. Wakabayashi, S., Takahashi, K. and Koide, T. (1999) Structural characterization of the gene for human histidine-rich glycoprotein, reinvestigation of the 5'-terminal region of cDNA and a search for the liver specific promoter in the gene. J Biochem (Tokyo), 125, 522-530. Walewski, J.L., Keller, T.R., Stump, D.D. and Branch, A.D. (2001) Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame. Rna, 7, 710-721. Wang, C., Sarnow, P. and Siddiqui, A. (1993) Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism. J Virol, 67, 3338-3344. Wang, Y.S., Youngster, S., Grace, M., Bausch, J., Bordens, R. and Wyss, D.F. (2002) Structural and biological characterization of pegylated recombinant interferon alpha-2b and its therapeutic implications. Adv Drug Deliv Rev, 54, 547-570. Weiner, A.J., Brauer, M.J., Rosenblatt, J., Richman, K.H., Tung, J., Crawford, K., Bonino, F., Saracco, G., Choo, Q.L., Houghton, M. and et al. (1991) Variable and hypervariable domains are found in the regions of HCV corresponding to the flavivirus envelope and NS1 proteins and the pestivirus envelope glycoproteins. Virology, 180, 842-848. Xu, Z., Choi, J., Yen, T.S., Lu, W., Strohecker, A., Govindarajan, S., Chien, D., Selby, M.J. and Ou, J. (2001) Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. EMBO J, 20, 3840-3848. Yamada, N., Tanihara, K., Takada, A., Yorihuzi, T., Tsutsumi, M., Shimomura, H., Tsuji, T. and Date, T. (1996) Genetic organization and diversity of the 3' noncoding region of the hepatitis C virus genome. Virology, 223, 255-261. Yasui, K., Wakita, T., Tsukiyama-Kohara, K., Funahashi, S.I., Ichikawa, M., Kajita, T., Moradpour, D., Wands, J.R. and Kohara, M. (1998) The native form and maturation process of hepatitis C virus core protein. J Virol, 72, 6048-6055. You, S., Stump, D.D., Branch, A.D. and Rice, C.M. (2004) A cis-acting replication element in the sequence encoding the NS5B RNA-dependent RNA polymerase is required for hepatitis C virus RNA replication. J Virol, 78, 1352-1366. 郭逸楨,C 型肝炎病毒非結構性蛋白質4B之核苷酸水解酶活性,2005年,國立臺灣大學醫學院生物化學暨分子生物學研究所碩士論文。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32431 | - |
dc.description.abstract | C型肝炎病毒 (Hepatitis C Virus,HCV) 是造成非A非B型肝炎的主要致病因子,屬於黃病毒科 (Flaviviridae),具外套膜,為一單股正向RNA病毒。根據本實驗室對HCV subgenomic replicon細胞株和其母細胞株Huh7進行比較之cDNA microarray分析,以及其他實驗室比較肝癌病人癌細胞和正常細胞基因表現差異的研究結果,顯示在HCV感染時一些細胞基因的表現可能會受到調控,例如:SAA、DUSP1、GRB14、VEGF-A、integrin、FASTK、H-ras、HRG、SULT2A1等。為確認此調節作用並釐清造成此現象的病毒蛋白質,本研究利用DNA轉染的方式,分別將HCV非結構性蛋白質之表現質體送至培養細胞中表現,並以即時定量聚合酵素鏈鎖反應 (real-time quantitative PCR) 分析不同病毒蛋白質對於細胞基因表現的影響。結果發現NS4B會造成FASTK與HRG表現量的下降。NS4B為病毒非結構性蛋白質之一,分子量 27 kD,具疏水性,其在病毒生活史的功能目前尚不清楚,但有研究指出NS4B對於NS5A的磷酸化,和病毒RNA複製是重要的;另外NS4B具有GTPase活性,有抑制宿主細胞轉譯的能力,也會與Ha-ras基因共同造成細胞轉型,可能在HCV的致病機轉中扮演重要角色。已知FASTK為一survival protein,因此嘗試分析NS4B對細胞存活的影響,以西方墨點法偵測細胞中caspase3的含量,發現在受到anti-Fas抗體刺激誘導細胞凋亡的293細胞中,NS4B會使細胞凋亡的情形更為嚴重。不過在nucleosome ELISA assay和flow cytometry的分析上,NS4B對細胞凋亡的影響則不明顯。細胞凋亡為幫助病毒顆粒散播的方式之一,NS4B是否藉由細胞凋亡調節C型肝炎病毒的傳播過程,則需進ㄧ步的釐清。另一方面,HRG為一含量豐富的血漿蛋白質,主要在肝臟細胞中表現,本研究發現在表現NS4B的Huh7細胞中,HRG的mRNA量和蛋白質量均有下降的情形。將NS4B表現質體與帶有長度約450 bp的HRG promoter之luciferase reporter進行共同轉染時,發現NS4B並不會影響luciferase的活性,顯示NS4B不會藉由此段HRG promoter調控HRG mRNA的合成。其調控機制仍有待進一步的研究。 | zh_TW |
dc.description.abstract | Hepatitis C virus (HCV) is the major infectious agent leading to non-A, non-B hepatitis. HCV belongs to the family Flaviviridae and is an enveloped, single-stranded RNA virus. Previously, a cDNA microarray analysis with HCV subgenomic replicon and patients parental Huh7 cells was performed in our lab to identify differentially expressed genes. According to the results and data from RT-PCR analysis of hepatocytes of HCC patients by another group, it is likely that HCV proteins are involved in the regulation of expression of several genes including SAA, DUSP1, GRB14, VEGF-A, integrin, FASTK, H-ras, HRG, and SULT2A1. In this study, cultured cells were transfected with expression plasmids of HCV nonstructural proteins. RT-PCR was applied to analyze the effects of the viral proteins on the expression of the cellular genes. NS4B down-regulated the expression of both FASTK and HRG mRNA. Nonstructural protein 4B (NS4B) is a relatively hydrophobic 27 kDa protein of unknown function. Nevertheless, NS4B was demonstrated to be important for the NS5A hyperphosphorylation and replication of HCV. In addition, NS4B has a GTPase activity. It inhibits translation of host cells, and causes malignant transformation in cooperation with the Ha-ras gene. Therefore, NS4B may play an important role in the pathogenicity of HCV. FASTK was shown to be a survival protein. To understand possible effects of the HCV NS4B protein on cellular survivability, western blot analysis was performed to detect the level of caspase3 in both anti-Fas antibody-treated and untreated 293 cells. Although NS4B seems to increase the severity of cell apoptosis, only limited effect was detected by nucleosome ELISA assay and flow cytometry. Apoptosis is one of gateways for viral particle spreads, the role of NS4B involved in the apoptosis and in the process of viral diffusion need to be further elucidated. On the other hand, HRG is an abundant plasma protein. It expresses mainly in the liver tissue. In this study, NS4B was found to down-regulate HRG both at the RNA and protein levels. When NS4B expression plasmid was cotransfected with a luciferase reporter carrying a 450 bp HRG promoter, no effect on the luciferase activity was detected. These results suggested that the regulatory effect of NS4B on HRG is not through a direct binding of the NS4B protein to the 450 bp promoter sequences. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:48:57Z (GMT). No. of bitstreams: 1 ntu-95-R93445120-1.pdf: 1484319 bytes, checksum: 212b941ca8b3161e8600250481cbbd59 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 目錄………………………………………………………1
中文摘要.……………………………………………….2 英文摘要…………………………………………………3 緒論………………………………………………………5 研究方向………………………………………………..18 材料與方法……………………………………………..19 實驗結果………………………………………………..32 討論……………………………………………………..38 圖表……………………………………………………..42 參考文獻………………………………………………..62 | |
dc.language.iso | zh-TW | |
dc.title | C型肝炎病毒非結構性蛋白質NS4B對細胞基因FASTK與HRG表現之調控 | zh_TW |
dc.title | Regulation of the gene expression of FASTK and HRG by the nonstructural protein 4B of hepatitis C virus | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王萬波,許金玉 | |
dc.subject.keyword | C型肝炎病毒,非結構性蛋白質NS4B, | zh_TW |
dc.subject.keyword | HCV,NS4B,FASTK,HRG, | en |
dc.relation.page | 73 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 1.45 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。